##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132655_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4496433
Sequences flagged as poor quality	0
Sequence length	100
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.450588277419012	31.0	30.0	34.0	16.0	34.0
2	31.045411996575954	33.0	31.0	34.0	25.0	34.0
3	31.82886701525409	34.0	31.0	34.0	28.0	34.0
4	35.902871231485044	37.0	35.0	37.0	35.0	37.0
5	35.64838461954176	37.0	35.0	37.0	35.0	37.0
6	36.08799953207353	37.0	36.0	37.0	35.0	37.0
7	36.13528101052545	37.0	35.0	37.0	35.0	37.0
8	36.28806189261577	37.0	37.0	37.0	35.0	37.0
9	38.195051944508016	39.0	39.0	39.0	37.0	39.0
10-11	38.24307011802466	39.0	39.0	39.0	37.0	39.0
12-13	38.211364541626665	39.0	39.0	39.0	37.0	39.0
14-15	39.73713063221447	41.0	40.0	41.0	37.5	41.0
16-17	39.59210133899471	41.0	39.5	41.0	37.0	41.0
18-19	39.3594416952282	41.0	39.0	41.0	35.5	41.0
20-21	39.2495770536334	41.0	39.0	41.0	35.0	41.0
22-23	38.73471727478203	40.5	39.0	41.0	35.0	41.0
24-25	38.55866338940222	41.0	39.0	41.0	35.0	41.0
26-27	38.53520056898435	41.0	38.5	41.0	35.0	41.0
28-29	38.41073780038533	41.0	39.0	41.0	35.0	41.0
30-31	38.21931417637047	41.0	38.0	41.0	34.5	41.0
32-33	38.08511769217955	40.0	38.0	41.0	34.0	41.0
34-35	37.977330030270664	40.0	38.0	41.0	34.0	41.0
36-37	37.758999411311144	40.0	38.0	41.0	34.0	41.0
38-39	37.35037728795247	40.0	36.0	41.0	33.0	41.0
40-41	37.20572573860213	40.0	35.0	41.0	33.0	41.0
42-43	37.092743737091155	40.0	35.0	41.0	33.0	41.0
44-45	36.922164413436164	40.0	35.0	41.0	33.0	41.0
46-47	36.79391964697351	40.0	35.0	41.0	33.0	41.0
48-49	36.62708973980041	40.0	35.0	41.0	32.5	41.0
50-51	36.35408378152193	40.0	35.0	41.0	31.0	41.0
52-53	35.78960822945655	39.0	35.0	41.0	29.0	41.0
54-55	34.77369038969334	39.0	35.0	41.0	22.5	41.0
56-57	34.23852028930487	39.0	35.0	41.0	13.5	41.0
58-59	33.94000299793191	39.0	35.0	41.0	7.0	41.0
60-61	33.68451069992592	38.5	35.0	41.0	2.0	41.0
62-63	33.471730702981674	38.0	35.0	40.0	2.0	41.0
64-65	33.16174042846852	37.5	34.0	40.0	2.0	41.0
66-67	32.744131070117135	36.5	34.0	40.0	2.0	41.0
68-69	32.463981115697706	36.0	34.0	39.5	2.0	41.0
70-71	32.171857447892585	36.0	33.0	39.0	2.0	41.0
72-73	31.864696304826516	35.0	33.0	39.0	2.0	41.0
74-75	31.709261986112104	35.0	33.0	38.5	2.0	40.0
76-77	31.401780255593714	35.0	33.0	37.5	2.0	40.0
78-79	31.04367728819711	35.0	33.0	37.0	2.0	39.0
80-81	30.708227721840846	35.0	33.0	36.5	2.0	39.0
82-83	30.309112467593756	35.0	32.5	36.0	2.0	38.5
84-85	29.928628203733936	35.0	31.5	36.0	2.0	37.0
86-87	29.76983377712956	35.0	32.0	35.0	2.0	37.0
88-89	29.525351317366457	35.0	31.5	35.0	2.0	36.5
90-91	29.29702477497163	35.0	31.0	35.0	2.0	36.0
92-93	29.169825726303493	35.0	31.5	35.0	2.0	36.0
94-95	28.91858012784801	35.0	31.0	35.0	2.0	36.0
96-97	28.599113897616178	35.0	30.5	35.0	2.0	35.5
98-99	28.451617426524535	35.0	31.0	35.0	2.0	35.0
100	28.394882343404205	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.3412425190371167
1101	2	2.055144382850127
1101	3	1.324344434518661
1101	4	0.6192305246782297
1101	5	0.8713216472695322
1101	6	0.42590834441264036
1101	7	0.27591976879107705
1101	8	0.17387558634555944
1101	9	0.21818416489836778
1101	10-11	0.15905146318614527
1101	12-13	0.1795648953444413
1101	14-15	0.25049780768267027
1101	16-17	0.23208703303539835
1101	18-19	0.1528601269506069
1101	20-21	0.20253451882597773
1101	22-23	-0.007978050770432787
1101	24-25	-0.01065364173157235
1101	26-27	0.06588054171245972
1101	28-29	0.05843264064007769
1101	30-31	0.09366979334010495
1101	32-33	0.11161392365870881
1101	34-35	0.09062922993718558
1101	36-37	0.12290301801395032
1101	38-39	0.0635786631740558
1101	40-41	0.07441314104703878
1101	42-43	0.10139215145360936
1101	44-45	0.15482199462947932
1101	46-47	0.19204106266803933
1101	48-49	0.20628209959019728
1101	50-51	0.17281010195566182
1101	52-53	0.20091161099628607
1101	54-55	0.23625097544253038
1101	56-57	0.11428259022203235
1101	58-59	0.08061448232747637
1101	60-61	0.0909592458508044
1101	62-63	0.14347277269899195
1101	64-65	0.2236985206460318
1101	66-67	0.24082915469913502
1101	68-69	0.14580700749774422
1101	70-71	0.17926835665704743
1101	72-73	0.1856006665442429
1101	74-75	0.19127381489038342
1101	76-77	0.1790272709131031
1101	78-79	0.09248333944434961
1101	80-81	0.19048791408583554
1101	82-83	0.2037487804490965
1101	84-85	0.22388628463555094
1101	86-87	0.14569366390371385
1101	88-89	0.20346865195237385
1101	90-91	0.1914323803330511
1101	92-93	0.19379631947266418
1101	94-95	0.22536539335060368
1101	96-97	0.19025600802256548
1101	98-99	0.2584553180317002
1101	100	0.2817070754356017
1102	1	3.3565420759267255
1102	2	2.0515210483901534
1102	3	1.3099069118437505
1102	4	0.6240197759177875
1102	5	0.8853653629470841
1102	6	0.4227687413824981
1102	7	0.2523425128506389
1102	8	0.22241617482344367
1102	9	0.21482595247205438
1102	10-11	0.22178383257091383
1102	12-13	0.16218234750618876
1102	14-15	0.20317042383247497
1102	16-17	0.24217407368434607
1102	18-19	0.18274294611256892
1102	20-21	0.16239101183513327
1102	22-23	0.21231200203691003
1102	24-25	0.22701430765027197
1102	26-27	0.27597657480464477
1102	28-29	0.30496977477942266
1102	30-31	0.35032962781713195
1102	32-33	0.4220989574423868
1102	34-35	0.42586131249401404
1102	36-37	0.41576673908696193
1102	38-39	0.44669085475650405
1102	40-41	0.41827099222520303
1102	42-43	0.35243946100762
1102	44-45	0.4197982886885754
1102	46-47	0.3795381556281967
1102	48-49	0.42066737306900137
1102	50-51	0.47191504376836235
1102	52-53	0.4402609922231733
1102	54-55	0.30091827054661024
1102	56-57	0.15188883283814647
1102	58-59	0.11319736009536285
1102	60-61	0.14615114148885056
1102	62-63	0.18363100869171944
1102	64-65	0.1573769260687783
1102	66-67	0.13446483051226465
1102	68-69	0.20254389791087135
1102	70-71	0.21148998627381843
1102	72-73	0.243261608022042
1102	74-75	0.25352942974094006
1102	76-77	0.28330937936196676
1102	78-79	0.30880276343184576
1102	80-81	0.3091680094677507
1102	82-83	0.25593363407382697
1102	84-85	0.18355595188414142
1102	86-87	0.23325913044097035
1102	88-89	0.3031002718635065
1102	90-91	0.35701892019593373
1102	92-93	0.28450371467193847
1102	94-95	0.313767312151942
1102	96-97	0.27809977517409834
1102	98-99	0.36374364987145924
1102	100	0.3024703168082823
1103	1	3.386042414567747
1103	2	2.0520401951073417
1103	3	1.3280708758104929
1103	4	0.6034027858579449
1103	5	0.900388990679545
1103	6	0.46614950502957697
1103	7	0.33075989446899
1103	8	0.2464157181594686
1103	9	0.2412476662270464
1103	10-11	0.18320961156097582
1103	12-13	0.21529884773221397
1103	14-15	0.2403781241745122
1103	16-17	0.2981690226104945
1103	18-19	0.21085439372195225
1103	20-21	0.2085588142477377
1103	22-23	0.08489388142516674
1103	24-25	0.1170377425347695
1103	26-27	0.16581988969142714
1103	28-29	0.19673655525210876
1103	30-31	0.2660450397635188
1103	32-33	0.2469729505831708
1103	34-35	0.3217110700455592
1103	36-37	0.30708495519702694
1103	38-39	0.3124883427960796
1103	40-41	0.30245923343635184
1103	42-43	0.28712185583991356
1103	44-45	0.3562417711216668
1103	46-47	0.3158832856000089
1103	48-49	0.368826369272206
1103	50-51	0.38560940905942687
1103	52-53	0.2952817503779386
1103	54-55	0.2385736095810458
1103	56-57	0.3293887981255068
1103	58-59	0.3277769746771355
1103	60-61	0.3086000946329648
1103	62-63	0.4372496078749677
1103	64-65	0.366157216698376
1103	66-67	0.39176104035552584
1103	68-69	0.36155552895722565
1103	70-71	0.3526859367751456
1103	72-73	0.3324591907885299
1103	74-75	0.39100225746291173
1103	76-77	0.41649133990064513
1103	78-79	0.38905952892409346
1103	80-81	0.41640921907188755
1103	82-83	0.44166203625902867
1103	84-85	0.4192601323651495
1103	86-87	0.42726418151482903
1103	88-89	0.4034625946169399
1103	90-91	0.4105026029440637
1103	92-93	0.3125495939737988
1103	94-95	0.3829426479773872
1103	96-97	0.39421160539383493
1103	98-99	0.419422713833729
1103	100	0.4198206166482983
1104	1	2.5793654905815515
1104	2	1.7818860398001668
1104	3	1.2425029362307676
1104	4	0.5743050213217913
1104	5	0.8784059157007817
1104	6	0.3794129276277687
1104	7	0.24753549602664293
1104	8	0.2072158078300248
1104	9	0.21932061067782627
1104	10-11	0.19523237769926283
1104	12-13	0.1896688363551604
1104	14-15	0.19839281976552314
1104	16-17	0.22666752232087362
1104	18-19	0.18822995670981157
1104	20-21	0.17625266083778257
1104	22-23	0.12831879390436995
1104	24-25	0.11301131211897797
1104	26-27	0.19947358048874975
1104	28-29	0.18772717067795952
1104	30-31	0.21590360488762883
1104	32-33	0.1981683104871621
1104	34-35	0.19418925219212468
1104	36-37	0.21195193852928185
1104	38-39	0.2792522693224626
1104	40-41	0.33221942587677944
1104	42-43	0.2948076673049087
1104	44-45	0.2923080369513542
1104	46-47	0.3212663063008563
1104	48-49	0.2830284318232472
1104	50-51	0.33986044260680615
1104	52-53	0.3722485614683393
1104	54-55	0.29481543946422306
1104	56-57	0.31881186854970167
1104	58-59	0.2817909431749257
1104	60-61	0.27301548489450767
1104	62-63	0.22499309937187917
1104	64-65	0.36989216600930064
1104	66-67	0.33254373020873373
1104	68-69	0.3139799862261441
1104	70-71	0.25090274040368854
1104	72-73	0.2840700096278148
1104	74-75	0.25642286717630824
1104	76-77	0.3097447875376247
1104	78-79	0.38787704041522986
1104	80-81	0.36088730422665094
1104	82-83	0.3581556536208197
1104	84-85	0.32739437307346364
1104	86-87	0.3427557269717454
1104	88-89	0.3822507303184466
1104	90-91	0.45098463299149216
1104	92-93	0.42346895248793714
1104	94-95	0.4909889695087841
1104	96-97	0.5328047224387262
1104	98-99	0.5148308571890041
1104	100	0.46021171070849576
1105	1	3.2892505859002696
1105	2	1.999597092435959
1105	3	1.2840504013419043
1105	4	0.5732709685856179
1105	5	0.8764827517461811
1105	6	0.3856509535401287
1105	7	0.22420535919774665
1105	8	0.17193076611126656
1105	9	0.1777758460511194
1105	10-11	0.13012928530935852
1105	12-13	0.10963169258863559
1105	14-15	0.16968639091779636
1105	16-17	0.12247689661490568
1105	18-19	0.1077792197848666
1105	20-21	0.0763314777350601
1105	22-23	0.10818526239893345
1105	24-25	0.10559790035860317
1105	26-27	0.13218826387929994
1105	28-29	0.1631247332020891
1105	30-31	0.1378965037310138
1105	32-33	0.12938357317817406
1105	34-35	0.16821643526839836
1105	36-37	0.16873150003392823
1105	38-39	0.18442196497041863
1105	40-41	0.18694477077985994
1105	42-43	0.2001115723730038
1105	44-45	0.13835707458250823
1105	46-47	0.1849086740707051
1105	48-49	0.14292839377434774
1105	50-51	0.17494979698057023
1105	52-53	0.11517589962690522
1105	54-55	0.09992452786075745
1105	56-57	0.09292312049987572
1105	58-59	0.1438354855238373
1105	60-61	0.08026168079658902
1105	62-63	0.08554310982926694
1105	64-65	0.09018644152241251
1105	66-67	0.11952097462324218
1105	68-69	0.09606974958362713
1105	70-71	0.15648565672945125
1105	72-73	0.20115575578190104
1105	74-75	0.17746342621756028
1105	76-77	0.15900493395602666
1105	78-79	0.11538379767911522
1105	80-81	0.14950229932760095
1105	82-83	0.134000034573873
1105	84-85	0.18232996000140034
1105	86-87	0.19500160812771128
1105	88-89	0.17559418053557607
1105	90-91	0.24466794848611073
1105	92-93	0.20864397274622348
1105	94-95	0.21236525463672962
1105	96-97	0.21853012004780936
1105	98-99	0.2750944207445336
1105	100	0.22410551685878488
1106	1	1.8270706750055545
1106	2	1.409471666944956
1106	3	1.053605889880341
1106	4	0.4796164732869812
1106	5	0.6820796069051696
1106	6	0.29706860936614987
1106	7	0.18224313308627416
1106	8	0.14593757178469247
1106	9	0.182924755626388
1106	10-11	0.13958753616980601
1106	12-13	0.08092171293507988
1106	14-15	0.1244234604838681
1106	16-17	0.09498645777119918
1106	18-19	0.09883640555813855
1106	20-21	0.12403258032836817
1106	22-23	-0.014577919955499397
1106	24-25	-0.0507528792125882
1106	26-27	-0.052631079104742184
1106	28-29	0.03373899725042406
1106	30-31	0.0804089715224805
1106	32-33	0.06557915698439842
1106	34-35	0.07072292608666686
1106	36-37	0.04608722961572198
1106	38-39	0.01835964984096705
1106	40-41	0.0224946559513981
1106	42-43	0.045626232327613536
1106	44-45	0.17179256456153524
1106	46-47	0.14097278265590063
1106	48-49	0.11026899175541871
1106	50-51	0.23662334765552373
1106	52-53	0.16742362170165848
1106	54-55	0.18172798220930986
1106	56-57	0.1592710698305666
1106	58-59	0.18173124172734845
1106	60-61	0.17345562539153292
1106	62-63	0.15521677364107944
1106	64-65	0.195064450153545
1106	66-67	0.2007119529224255
1106	68-69	0.20880031141547306
1106	70-71	0.18258816818592294
1106	72-73	0.17306057130151586
1106	74-75	0.15724353144575431
1106	76-77	0.16037326247346684
1106	78-79	0.1596194472138528
1106	80-81	0.17907535682040532
1106	82-83	0.18110471309238108
1106	84-85	0.21401405677816498
1106	86-87	0.19034130610685196
1106	88-89	0.18872446836521561
1106	90-91	0.15308497642072894
1106	92-93	0.12284175781019258
1106	94-95	0.21823322901032327
1106	96-97	0.23557927237622778
1106	98-99	0.16394668364549858
1106	100	0.22520951073557072
1107	1	2.852519426065875
1107	2	1.8401618328718818
1107	3	1.2073073885937724
1107	4	0.5208356801718779
1107	5	0.8360463791464099
1107	6	0.4004149207876111
1107	7	0.21441234630493256
1107	8	0.1781271514504823
1107	9	0.07065931438010864
1107	10-11	0.12441336958298876
1107	12-13	0.12672366960325832
1107	14-15	0.14969640411761276
1107	16-17	0.18976839007765278
1107	18-19	0.12170610843140395
1107	20-21	0.13812168510799694
1107	22-23	-0.00319881585046744
1107	24-25	-0.021523448504204623
1107	26-27	0.029546980997153582
1107	28-29	0.030265864250836216
1107	30-31	0.07123650215437038
1107	32-33	0.04846060413947839
1107	34-35	0.0573745815663429
1107	36-37	0.06595729168308395
1107	38-39	0.08712977306527847
1107	40-41	0.08393806617135624
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2216	88-89	-0.6257021938845
2216	90-91	-0.6658876812941266
2216	92-93	-0.6394622898557571
2216	94-95	-0.7061670089169425
2216	96-97	-0.8009442747988089
2216	98-99	-0.8985906763139084
2216	100	-0.9502067361979449
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	695.0
3	177.0
4	316.0
5	899.0
6	2867.0
7	5949.0
8	22645.0
9	32183.0
10	5349.0
11	12287.0
12	9342.0
13	13545.0
14	12497.0
15	13585.0
16	16188.0
17	19643.0
18	25646.0
19	41566.0
20	89879.0
21	117928.0
22	52712.0
23	28963.0
24	26259.0
25	26744.0
26	29283.0
27	32424.0
28	38104.0
29	45130.0
30	52041.0
31	63853.0
32	82038.0
33	111653.0
34	165887.0
35	399322.0
36	472400.0
37	836174.0
38	1115698.0
39	468156.0
40	6406.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.056565020284964	45.90800968253387	24.961678508111753	16.073746789069407
2	15.088856358857546	44.561376118182764	23.468994239337704	16.880773283621988
3	16.336377904283385	24.91262491329211	20.231566497695166	38.519430684729336
4	35.21249741453509	26.30026088174918	21.706228913170637	16.781012790545095
5	15.320112916272002	43.38754153573869	22.553230574751655	18.73911497323765
6	38.043911696226765	23.240955664189812	23.633533514232283	15.081599125351138
7	17.324221221577194	23.303694283891254	42.946508932747356	16.425575561784196
8	37.58781683169748	23.75111560652633	22.73842399075	15.922643571026187
9	37.09493725359635	25.420661221906343	22.87935792660538	14.605043597891928
10-11	14.885032202192273	46.37440388859347	23.037638946249174	15.702924962965087
12-13	14.647288194886926	24.69688751061119	33.63424963743483	27.02157465706706
14-15	15.026344274104106	35.18869332355964	22.891407236374338	26.893555165961917
16-17	26.33889199937951	24.083570697436247	33.53647586169702	16.041061441487223
18-19	15.625741560032141	24.47613474947809	33.72525510777098	26.172868582718788
20-21	26.443558258740723	35.343797648976164	23.18714827605226	15.025495816230855
22-23	25.79124706426589	24.699465340545157	33.639287239342394	15.870000355846559
24-25	15.119273432963418	35.31116776342492	22.7056424503601	26.863916353251565
26-27	15.616033865065932	30.452560952203665	38.10053435690023	15.830870825830162
28-29	28.000150341392832	24.369227785669217	32.00515831104344	15.625463561894506
30-31	23.132603110065244	27.47620169142963	32.721148074484816	16.670047124020307
32-33	17.489318755555793	28.85315982691169	33.68045292790974	19.977068489622773
34-35	19.19960145207556	28.7483493454929	33.700941158024136	18.3511080444074
36-37	19.649555313882345	27.298156537519763	35.486163467461964	17.56612468113592
38-39	21.50925062997291	27.161731985432425	34.01978261166903	17.309234772925638
40-41	17.166222017813233	31.49091323965313	33.71572171001363	17.627143032520006
42-43	17.057716440125457	27.469215804342227	35.71971939914454	19.75334835638777
44-45	17.192579283880498	29.568038224710065	33.300692104510794	19.938690386898642
46-47	19.58081383911926	27.183148663400836	35.15667299883206	18.07936449864785
48-49	17.603142024623825	27.674140936738162	35.04811083423138	19.67460620440663
50-51	19.6036515458933	29.860452713583786	32.93177848842084	17.60411725210207
52-53	19.545784505512394	27.9176485606746	34.19291870940652	18.343648224406483
54-55	18.06142643051877	30.48799161199261	30.693966610544233	20.756615346944386
56-57	18.58534457384008	31.154765133523547	30.90999243508692	19.34989785754945
58-59	22.789671269618516	29.717962632244976	28.40538743302961	19.086978665106898
60-61	20.55049234146009	31.753897436804163	28.198212293807796	19.49739792792796
62-63	18.381311099956566	31.72356115071914	28.037798045246188	21.857329704078108
64-65	20.29573950388417	31.650427526351976	28.321690102692394	19.73214286707146
66-67	20.478395720688265	29.67217949196962	30.309130099830444	19.540294687511675
68-69	22.331616634652317	30.00676538073281	28.605795587201467	19.05582239741341
70-71	17.908971933496556	33.717290190674035	28.672262997992696	19.70147487783671
72-73	17.859341826079405	30.085097595856976	30.439846051046548	21.615714527017072
74-75	18.148411855503475	31.948648554579957	28.092541933021703	21.81039765689487
76-77	20.404096498591702	29.8118051279736	29.76167587389535	20.022422499539353
78-79	18.925575896892614	30.20438908953708	29.262224141782777	21.607810871787528
80-81	20.775473561900075	32.06913381566073	27.69999756467701	19.455395057762193
82-83	20.34842182693222	30.583803661470494	29.215903549881656	19.85187096171563
84-85	18.845162960488537	32.4569997193289	27.16906274978505	21.528774570397516
86-87	18.836653631020255	32.63819153036621	28.817419324561794	19.70773551405174
88-89	22.883755449463717	30.490630770004053	27.261819348025963	19.36379443250627
90-91	20.487360927499925	32.60202579235517	27.19120923016154	19.719404049983364
92-93	18.604269693834645	32.71377188613157	26.84695468845274	21.83500373158105
94-95	20.115068497416093	33.0110272215941	26.6228206694488	20.251083611541013
96-97	20.533782145804004	30.907722109907905	28.371720366255886	20.18677537803221
98-99	22.454214837067145	31.15717381042532	26.50876282899181	19.879848523515715
100	18.757396410223677	34.59770756598415	26.552526105533275	20.092369918258896
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	190924.0
1	105670.0
2	13711.5
3	5627.5
4	3895.0
5	3428.5
6	3442.5
7	3837.0
8	3719.5
9	3372.0
10	3482.0
11	4391.0
12	27789.0
13	30899.5
14	10354.0
15	9869.5
16	11469.0
17	13386.5
18	15458.0
19	17842.0
20	20689.0
21	23936.0
22	27703.5
23	32471.5
24	37108.5
25	42141.5
26	49431.0
27	56386.0
28	61497.0
29	65771.5
30	70119.0
31	75741.5
32	82437.5
33	89671.5
34	97473.0
35	104714.5
36	111168.5
37	117269.5
38	121159.5
39	122130.5
40	122397.0
41	122391.5
42	120046.5
43	117532.5
44	116844.5
45	117559.5
46	121456.0
47	133036.5
48	139154.5
49	172820.5
50	325464.0
51	322882.0
52	151725.0
53	93003.0
54	82475.5
55	80832.0
56	75234.5
57	62472.5
58	53967.0
59	42485.0
60	31264.5
61	24978.0
62	18786.5
63	13351.5
64	9522.5
65	6922.5
66	5194.5
67	4048.5
68	3029.0
69	2192.0
70	1606.0
71	1356.5
72	1226.0
73	912.5
74	647.5
75	498.0
76	356.0
77	237.0
78	166.5
79	102.0
80	59.0
81	31.5
82	17.0
83	8.5
84	4.0
85	2.0
86	2.0
87	1.5
88	1.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	1.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.29599017710260556
2	0.0016679888258092582
3	3.1135791415106155E-4
4	0.0031802986945429854
5	8.895940404316043E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.2239851010790108E-4
16-17	1.1119925505395054E-5
18-19	0.0
20-21	2.0015865909711096E-4
22-23	0.0025131031642192823
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0024575035366923067
36-37	6.671955303237033E-5
38-39	2.1127858460250604E-4
40-41	2.223985101079011E-5
42-43	2.3351843561329614E-4
44-45	0.0016568689003038633
46-47	0.0017235884533362334
48-49	0.0021461456225412455
50-51	8.006346363884438E-4
52-53	4.6703687122659227E-4
54-55	3.0023798864566644E-4
56-57	3.669575416780368E-4
58-59	9.563135934639747E-4
60-61	0.0025019832387138874
62-63	2.2239851010790108E-4
64-65	9.674335189693696E-4
66-67	2.0015865909711096E-4
68-69	2.668782121294813E-4
70-71	0.0021016659205196652
72-73	1.6679888258092583E-4
74-75	1.4455903157013572E-4
76-77	0.0012565515821096412
78-79	7.450350088614686E-4
80-81	0.002435263685681517
82-83	3.447176906672467E-4
84-85	0.0014900700177229373
86-87	0.006671955303237033
88-89	0.002646542270284023
90-91	0.004826047669341454
92-93	0.0077728279282711435
94-95	0.0053709240191058116
96-97	0.0027355016743271835
98-99	5.671162007751477E-4
100	0.003647335565769578
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4496433.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.47511007393718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.15803959153621	41.63728272398017
2	10.898824224952392	10.784410564120254
3	2.491650930837713	3.6982411220707165
4	0.750568785585276	1.4853789313957155
5	0.3347750718392173	0.8281516764627752
6	0.2183749408136904	0.648247454048811
7	0.14250627310744962	0.49353594837523374
8	0.12186399463691938	0.4823387638969021
9	0.09464094319423762	0.42141339738325284
>10	0.6372160428036607	6.276200215316435
>50	0.10279437467561642	3.3174551805724715
>100	0.04081302934914225	4.1035167091430225
>500	0.00333495997517789	1.0453484243376139
>1k	0.0025237534786025136	3.147773457914812
>5k	0.001757614029741036	6.326227534055873
>10k+	3.154691848253142E-4	15.304477896925953
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	312450	6.948841448321369	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	177580	3.9493527424961075	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	136170	3.028400512139289	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18333	0.4077231885808151	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17916	0.39844917070931557	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	13802	0.3069544236509251	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	10142	0.2255565689514333	No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA	9392	0.2088766806933407	No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT	9291	0.2066304557412509	No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC	9255	0.20582982110486245	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG	9199	0.2045843894482582	No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG	9072	0.20175992836988788	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	8907	0.19809035295310748	No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT	8593	0.1911070397357194	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8435	0.18759314327601456	No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT	8381	0.1863921913214319	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	8371	0.186169792811324	No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA	8358	0.18588067474818373	No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG	8337	0.18541363787695714	No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT	8329	0.18523571906887082	No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC	8311	0.1848354017506766	No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC	8122	0.18063206990963726	No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT	7901	0.17571706283625266	No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA	7852	0.17462731013672392	No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG	7618	0.16942318500019904	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7484	0.16644304496475318	No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA	7475	0.16624288630565606	No Hit
TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA	7216	0.16048276489386143	No Hit
CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT	7117	0.15828101964379318	No Hit
ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG	6877	0.15294345540120358	No Hit
GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT	6858	0.15252089823199858	No Hit
GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC	6616	0.14713885428738735	No Hit
TACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	6352	0.14126753362053876	No Hit
CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC	6339	0.14097841555739848	No Hit
GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT	6259	0.1391992274765353	No Hit
GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT	6168	0.1371754010345534	No Hit
CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG	6117	0.13604116863300308	No Hit
ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG	5945	0.1322159142591472	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	5706	0.12690058986756836	No Hit
TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG	5606	0.12467660476648935	No Hit
ACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATC	5470	0.1216519850290219	No Hit
CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT	5382	0.11969487814007236	No Hit
TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA	5375	0.11953919918299682	No Hit
ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC	5350	0.11898320290772708	No Hit
ACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAAC	5175	0.1150912289808388	No Hit
CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG	5115	0.11375683792019141	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4984	0.11084341743777791	No Hit
ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC	4915	0.10930886771803339	No Hit
GAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATG	4811	0.10699592321291121	No Hit
GGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGC	4657	0.10357098615724954	No Hit
TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC	4612	0.10257019286176397	No Hit
TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG	4600	0.1023033146496345	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.001868147484906369	0.0	0.0	0.0	0.0
2	0.001868147484906369	0.0	0.0	0.0	0.0
3	0.001868147484906369	0.0	0.0	0.0	0.0
4	0.001868147484906369	0.0	0.0	0.0	0.0
5	0.001868147484906369	0.0	0.0	0.0	0.0
6	0.0018903873359171592	0.0	0.0	0.0	0.0
7	0.0018903873359171592	0.0	0.0	0.0	0.0
8	0.0018903873359171592	0.0	0.0	0.0	0.0
9	0.0019126271869279493	0.0	0.0	0.0	0.0
10-11	0.0019459869634441346	0.0	0.0	0.0	0.0
12-13	0.00204606629299269	0.0	0.0	0.0	0.0
14-15	0.0021683854735520354	0.0	0.0	0.0	0.0
16-17	0.0023240644306275664	0.0	0.0	0.0	0.0
18-19	0.002468623462197702	0.0	0.0	0.0	0.0
20-21	0.0026131824937678377	0.0	0.0	0.0	0.0
22-23	0.0029356603334242943	0.0	0.0	0.0	0.0
24-25	0.003280378024091541	0.0	0.0	0.0	0.0
26-27	0.0035806160127372076	0.0	0.0	0.0	0.0
28-29	0.003903093852393664	0.0	0.0	0.0	0.0
30-31	0.004314531096093281	0.0	0.0	0.0	0.0
32-33	0.004948366849900799	0.0	0.0	0.0	0.0
34-35	0.005593322529213712	0.0	0.0	0.0	0.0
36-37	0.006382837240096761	0.0	0.0	0.0	0.0
38-39	0.007772827928271143	0.0	0.0	0.0	0.0
40-41	0.009863373923285412	0.0	0.0	0.0	0.0
42-43	0.0121429586518914	0.0	0.0	0.0	0.0
44-45	0.014800620847680818	0.0	0.0	0.0	0.0
46-47	0.017369323639427073	0.0	0.0	0.0	0.0
48-49	0.020204904643302813	0.0	0.0	0.0	0.0
50-51	0.023907839836599366	0.0	0.0	0.0	0.0
52-53	0.028678287878413845	0.0	0.0	0.0	0.0
54-55	0.034683047651327174	0.0	0.0	0.0	0.0
56-57	0.041555161613661316	0.0	0.0	0.0	0.0
58-59	0.04893879214924363	0.0	0.0	0.0	0.0
60-61	0.05853528786039956	0.0	0.0	0.0	0.0
62-63	0.07107856383048519	0.0	0.0	0.0	0.0
64-65	0.08622390236883325	0.0	0.0	0.0	0.0
66-67	0.10420482191105705	0.0	0.0	0.0	0.0
68-69	0.12579971724253425	0.0	0.0	0.0	0.0
70-71	0.1562349533508005	0.0	0.0	0.0	0.0
72-73	0.19228575183929128	0.0	0.0	0.0	0.0
74-75	0.23513082481157843	0.0	0.0	0.0	0.0
76-77	0.28438097487497316	1.1119925505395054E-5	0.0	0.0	0.0
78-79	0.345851923068797	2.223985101079011E-5	0.0	0.0	0.0
80-81	0.4255706690169741	2.223985101079011E-5	0.0	0.0	0.0
82-83	0.525127362066776	2.223985101079011E-5	0.0	1.1119925505395054E-5	0.0
84-85	0.6460787917889581	2.223985101079011E-5	0.0	2.223985101079011E-5	0.0
86-87	0.787913441610272	2.223985101079011E-5	0.0	4.447970202158022E-5	0.0
88	0.9095209469372723	2.223985101079011E-5	0.0	6.671955303237033E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	5590	0.0	87.89205	1
AACGTGT	1555	0.0	64.58668	1
CGTGTGG	1775	0.0	59.310406	3
ACGTGTG	2085	0.0	49.142883	2
ATCGAGT	1015	0.0	46.919037	1
GTGGAAT	197235	0.0	46.58322	6
TGGAATC	199540	0.0	46.063953	7
GAATCAC	200860	0.0	45.761234	9
GGAATCA	200905	0.0	45.732265	8
AGTGGAA	198930	0.0	45.713806	5
TCGAGTG	1170	0.0	44.59101	2
ACGAGTG	205660	0.0	43.630383	2
ATCTGCG	2680	0.0	43.492805	94
CGAGTGG	208425	0.0	43.355373	3
AACGAGT	196510	0.0	43.101063	1
GAGTGGA	211240	0.0	43.049053	4
AGTACTT	49520	0.0	40.279644	20-21
GAGTACT	49030	0.0	39.627754	20-21
GTACTTT	49205	0.0	38.683395	22-23
TACTTTT	52495	0.0	37.360203	22-23
>>END_MODULE