FastQCFastQC Report
Thu 26 May 2016
SRR2132654_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132654_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5090666
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3642517.155272021381879No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1101782.1643140602820927No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG360680.7085124028958097No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT206390.40542828777216966No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94110.1848677560067779No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74580.1465034241099298No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA70450.13839053671955692No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC67940.13345994414090415No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA67320.13224202884259154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT50700.087.29611
AACGAGT1098250.071.101681
ACGAGTG1171100.070.530172
GTGGAAT1204100.070.153716
CGAGTGG1193350.069.5689243
TGGAATC1215600.069.474567
GAATCAC1220400.069.1859059
GGAATCA1222800.069.100088
AGTGGAA1212200.068.831995
GAGTGGA1224500.068.156124
AACGTGT19150.049.9648321
CGTGTGG21800.045.9216163
ATCGAGT8400.044.889821
AGTACTT546750.041.67344320-21
GAGTACT538250.041.6417320-21
ACGTGTG24200.040.9793852
GTACTTT547100.040.26291722-23
TACTTTT587000.038.28674722-23
CATGGGG252500.038.18375424-25
ACTTTTT597300.036.4180124-25
Produced by FastQC (version 0.11.5)