##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132654_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5090666
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.438818221427216	31.0	30.0	34.0	16.0	34.0
2	31.03959678360356	33.0	31.0	34.0	25.0	34.0
3	31.82007973023569	34.0	31.0	34.0	28.0	34.0
4	35.90430328762484	37.0	35.0	37.0	35.0	37.0
5	35.65453970069928	37.0	35.0	37.0	35.0	37.0
6	36.10328943207038	37.0	36.0	37.0	35.0	37.0
7	36.1531577597116	37.0	35.0	37.0	35.0	37.0
8	36.297927815338895	37.0	37.0	37.0	35.0	37.0
9	38.2061814701652	39.0	39.0	39.0	37.0	39.0
10-11	38.252407445312656	39.0	39.0	39.0	37.0	39.0
12-13	38.218483298648934	39.0	39.0	39.0	37.0	39.0
14-15	39.74010875983614	41.0	40.0	41.0	37.5	41.0
16-17	39.646188239417	41.0	40.0	41.0	37.0	41.0
18-19	39.53540047608702	41.0	39.5	41.0	36.5	41.0
20-21	39.478604665872794	41.0	39.5	41.0	36.0	41.0
22-23	39.198464798122686	40.5	39.0	41.0	36.0	41.0
24-25	39.158618734758875	41.0	39.0	41.0	35.0	41.0
26-27	39.08054938194728	41.0	39.0	41.0	35.0	41.0
28-29	38.94402647905009	41.0	39.0	41.0	35.0	41.0
30-31	38.80306633748904	41.0	39.0	41.0	35.0	41.0
32-33	38.677217872867715	41.0	39.0	41.0	35.0	41.0
34-35	38.59865722873981	41.0	39.0	41.0	35.0	41.0
36-37	38.45007932164475	40.5	38.0	41.0	35.0	41.0
38-39	38.03549044466874	40.0	37.0	41.0	34.5	41.0
40-41	37.84438195316683	40.0	36.0	41.0	34.0	41.0
42-43	37.71610757806542	40.0	36.0	41.0	34.0	41.0
44-45	37.54983856729159	40.0	35.0	41.0	33.0	41.0
46-47	37.438841990419334	40.0	35.0	41.0	33.0	41.0
48-49	37.2897019761265	40.0	35.0	41.0	33.0	41.0
50-51	37.051215008016634	40.0	35.0	41.0	33.0	41.0
52-53	36.558340991139474	40.0	35.0	41.0	31.5	41.0
54-55	35.67938684250744	39.5	35.0	41.0	28.0	41.0
56-57	35.02514779009269	39.0	35.0	41.0	23.5	41.0
58-59	34.666019141699735	39.0	35.0	41.0	20.5	41.0
60-61	34.41381167022154	39.0	35.0	41.0	20.0	41.0
62-63	34.13823869018317	38.0	35.0	40.5	18.0	41.0
64-65	33.84412471766956	37.5	35.0	40.0	17.0	41.0
66-67	33.51057445135862	37.0	34.0	40.0	13.5	41.0
68-69	33.20588131297555	36.5	34.0	39.5	10.5	41.0
70-71	32.87817979022784	36.0	34.0	39.0	7.5	41.0
72-73	32.53438312393702	35.5	34.0	39.0	6.0	41.0
74-75	32.346469204618806	35.0	34.0	38.5	2.0	40.0
76-77	32.042290733668246	35.0	34.0	37.0	2.0	39.5
78-79	31.707710346740484	35.0	34.0	37.0	2.0	39.0
80-81	31.40320627988558	35.0	34.0	36.5	2.0	39.0
82-83	31.098394984074776	35.0	33.0	36.0	2.0	38.0
84-85	30.793914784430957	35.0	33.0	36.0	2.0	37.0
86-87	30.58078677721147	35.0	33.0	35.0	2.0	37.0
88-89	30.371029350580063	35.0	33.0	35.0	2.0	36.5
90-91	30.189396240099036	35.0	33.0	35.0	2.0	36.0
92-93	30.03541088729844	35.0	33.0	35.0	2.0	36.0
94-95	29.861534816858935	35.0	33.0	35.0	2.0	36.0
96-97	29.67624688007424	35.0	33.0	35.0	2.0	35.5
98-99	29.54562654473894	35.0	33.0	35.0	2.0	35.0
100	29.45767390750051	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.434058315636058
1101	2	2.082036173743756
1101	3	1.3700128721364706
1101	4	0.6353168904494382
1101	5	0.9133252599289676
1101	6	0.4419485883547196
1101	7	0.2898350261052727
1101	8	0.21368751505704608
1101	9	0.24368146186959194
1101	10-11	0.17553036527345967
1101	12-13	0.2420141624284753
1101	14-15	0.3178956572885454
1101	16-17	0.3110971029287555
1101	18-19	0.22852493297926202
1101	20-21	0.2782473470627167
1101	22-23	0.23263939717951843
1101	24-25	0.27417585608163364
1101	26-27	0.2836593147849129
1101	28-29	0.32033942364775925
1101	30-31	0.34078452970766193
1101	32-33	0.3027603111914061
1101	34-35	0.3004981558920363
1101	36-37	0.3364451925481191
1101	38-39	0.3398707273653656
1101	40-41	0.34802953631621847
1101	42-43	0.33582092683061404
1101	44-45	0.3332539027329773
1101	46-47	0.25090813905126197
1101	48-49	0.29977885143678407
1101	50-51	0.23545499252428925
1101	52-53	0.2778334935820439
1101	54-55	0.31041384050476495
1101	56-57	0.1912241757966484
1101	58-59	0.19230612852290108
1101	60-61	0.22084946316307708
1101	62-63	0.2691363350318028
1101	64-65	0.26480081435360603
1101	66-67	0.28533292530272547
1101	68-69	0.27032028716892853
1101	70-71	0.24733243285614748
1101	72-73	0.27420050581145716
1101	74-75	0.29170354618489114
1101	76-77	0.3070933217011387
1101	78-79	0.1700057145167726
1101	80-81	0.2105904189010559
1101	82-83	0.18262043013524476
1101	84-85	0.21983767322397796
1101	86-87	0.178464714200274
1101	88-89	0.16986453468993545
1101	90-91	0.1971023958107878
1101	92-93	0.17147229512121953
1101	94-95	0.19679957354289002
1101	96-97	0.12383110182753043
1101	98-99	0.17803052678495845
1101	100	0.16951942561200894
1102	1	3.369727160378382
1102	2	2.0508932098208312
1102	3	1.3391033572216813
1102	4	0.6485876807073367
1102	5	0.898645491520675
1102	6	0.4343030624126172
1102	7	0.27501845654037993
1102	8	0.21368787441304704
1102	9	0.21814677796291448
1102	10-11	0.18913424071991614
1102	12-13	0.18848446373524297
1102	14-15	0.20307193843052573
1102	16-17	0.27354232950839474
1102	18-19	0.163727409282572
1102	20-21	0.16998983784618105
1102	22-23	0.16716325803989918
1102	24-25	0.15292623718812592
1102	26-27	0.18814684853600738
1102	28-29	0.25388378406594114
1102	30-31	0.271366349773956
1102	32-33	0.2508542302069401
1102	34-35	0.2479865978681488
1102	36-37	0.2441816071889562
1102	38-39	0.23408287337132094
1102	40-41	0.28411812778442425
1102	42-43	0.24829350229348535
1102	44-45	0.2610932123457843
1102	46-47	0.236907752081585
1102	48-49	0.2791969821954723
1102	50-51	0.24431585101443432
1102	52-53	0.22257993576847213
1102	54-55	0.14166123517373563
1102	56-57	0.04719279531731502
1102	58-59	0.04133645363038596
1102	60-61	0.09271697579160332
1102	62-63	0.07761549124877831
1102	64-65	0.05710529064329961
1102	66-67	0.04368763086154814
1102	68-69	0.0963921515608348
1102	70-71	0.12212341059952792
1102	72-73	0.06332996778365185
1102	74-75	0.1034241597531178
1102	76-77	0.12045788496254062
1102	78-79	0.10283277198120544
1102	80-81	0.16656160119744
1102	82-83	0.15598106695947322
1102	84-85	0.1361204814814485
1102	86-87	0.1595669952408798
1102	88-89	0.1293339720292188
1102	90-91	0.16051072485075935
1102	92-93	0.13666094945781637
1102	94-95	0.1287094504615709
1102	96-97	0.1766657965527152
1102	98-99	0.195018363151636
1102	100	0.1970210540621089
1103	1	3.3758560425159345
1103	2	2.0718224349884657
1103	3	1.3680524471090294
1103	4	0.6182431286513506
1103	5	0.8904887220913267
1103	6	0.4493430435635233
1103	7	0.2922770110725068
1103	8	0.25248210319538344
1103	9	0.24583511523290014
1103	10-11	0.18565630498829222
1103	12-13	0.18744640993337214
1103	14-15	0.2495133672152008
1103	16-17	0.3117787319658021
1103	18-19	0.2531352579981103
1103	20-21	0.23707095041097404
1103	22-23	0.2679848326416234
1103	24-25	0.2680561832457684
1103	26-27	0.2695612841900612
1103	28-29	0.27537500635938983
1103	30-31	0.30734623919650517
1103	32-33	0.25129348139451935
1103	34-35	0.33247275519953234
1103	36-37	0.3411111294347009
1103	38-39	0.3007195761263972
1103	40-41	0.30915339666205455
1103	42-43	0.31407497135199947
1103	44-45	0.41302590293675223
1103	46-47	0.29623860195909657
1103	48-49	0.3383219628048977
1103	50-51	0.34393649106407054
1103	52-53	0.34086706833554814
1103	54-55	0.31969271228116014
1103	56-57	0.321699250546331
1103	58-59	0.33094091536291614
1103	60-61	0.2961780088038495
1103	62-63	0.33068073720895086
1103	64-65	0.3203869133628885
1103	66-67	0.27774843967495855
1103	68-69	0.2895539405856269
1103	70-71	0.3329411875772905
1103	72-73	0.35444360088780513
1103	74-75	0.4222416059936904
1103	76-77	0.4533393083617554
1103	78-79	0.49492888479478125
1103	80-81	0.4484343859167943
1103	82-83	0.4238869005196406
1103	84-85	0.4369862885241034
1103	86-87	0.4259548036240446
1103	88-89	0.3711107990595188
1103	90-91	0.39492927130470434
1103	92-93	0.3622399590609007
1103	94-95	0.3977582663931507
1103	96-97	0.42438941733471935
1103	98-99	0.4116630782347137
1103	100	0.4433094803091784
1104	1	2.438584510305237
1104	2	1.7130720215074398
1104	3	1.159275361650181
1104	4	0.5163470212347576
1104	5	0.8249374699828849
1104	6	0.3133276147831552
1104	7	0.21244826085949597
1104	8	0.15173593427779508
1104	9	0.15141664061725635
1104	10-11	0.11697919441188276
1104	12-13	0.11493567468021126
1104	14-15	0.11246370925135096
1104	16-17	0.11108390224735132
1104	18-19	0.1160092413287046
1104	20-21	0.07614104701263358
1104	22-23	0.05477836117142232
1104	24-25	0.0415402532036353
1104	26-27	0.08895901756586966
1104	28-29	0.1268723415586024
1104	30-31	0.1448239344645117
1104	32-33	0.0828159966259463
1104	34-35	0.1374887031827825
1104	36-37	0.1481942101759799
1104	38-39	0.1860641923164934
1104	40-41	0.17741746929002744
1104	42-43	0.11377395643675214
1104	44-45	0.12377289772685174
1104	46-47	0.20024265272940767
1104	48-49	0.14608818611530694
1104	50-51	0.18932828948899783
1104	52-53	0.2352027087368569
1104	54-55	0.19813074257012886
1104	56-57	0.2505531842780897
1104	58-59	0.2457786230604171
1104	60-61	0.21698956401695568
1104	62-63	0.11912009092775833
1104	64-65	0.24372437097427024
1104	66-67	0.21641024509765572
1104	68-69	0.19815010040190373
1104	70-71	0.08831938271244866
1104	72-73	0.11870163251618493
1104	74-75	0.16465018873398662
1104	76-77	0.2713933827460764
1104	78-79	0.3331575284360966
1104	80-81	0.27088944559534767
1104	82-83	0.28777531969505077
1104	84-85	0.255287125916702
1104	86-87	0.2362065618172835
1104	88-89	0.23115155663085218
1104	90-91	0.3060691112060141
1104	92-93	0.25050845086951057
1104	94-95	0.1990981555707947
1104	96-97	0.27203466012743505
1104	98-99	0.2889181750738956
1104	100	0.23927985436623445
1105	1	3.352464880558294
1105	2	2.032612126634124
1105	3	1.3313420901365518
1105	4	0.605220059010172
1105	5	0.8912246189334141
1105	6	0.43335357343133296
1105	7	0.2584994849296649
1105	8	0.19206638292009615
1105	9	0.1886202488130806
1105	10-11	0.1396320143018812
1105	12-13	0.1327111750102148
1105	14-15	0.21484490047858174
1105	16-17	0.1560209883086543
1105	18-19	0.1473592941178552
1105	20-21	0.18539979746032742
1105	22-23	0.17305568938142102
1105	24-25	0.18915005306586608
1105	26-27	0.2648049885339816
1105	28-29	0.2880641246530331
1105	30-31	0.3164259510608929
1105	32-33	0.2703353152832122
1105	34-35	0.26802599532900473
1105	36-37	0.21919945814141073
1105	38-39	0.2940498186763705
1105	40-41	0.27022783383329596
1105	42-43	0.2983962565546463
1105	44-45	0.2604925118980361
1105	46-47	0.27238991312061955
1105	48-49	0.18327379452590264
1105	50-51	0.2490946645411114
1105	52-53	0.21311498831659037
1105	54-55	0.25610174726079293
1105	56-57	0.17365470202069844
1105	58-59	0.1928175285320748
1105	60-61	0.15288387901745182
1105	62-63	0.19021524764005449
1105	64-65	0.20320970408915429
1105	66-67	0.14939799157252054
1105	68-69	0.1370687580738803
1105	70-71	0.1231420413615254
1105	72-73	0.16410016597736643
1105	74-75	0.21850587078462524
1105	76-77	0.1978597601392309
1105	78-79	0.14306920035173576
1105	80-81	0.16889862245578868
1105	82-83	0.15423318163871613
1105	84-85	0.12367480651740692
1105	86-87	0.12242994643846572
1105	88-89	0.09135431284886764
1105	90-91	0.15054624143279938
1105	92-93	0.11454237480140961
1105	94-95	0.15814528950539142
1105	96-97	0.1513798542731557
1105	98-99	0.1309942006838014
1105	100	0.1314431410592043
1106	1	1.7263169754708976
1106	2	1.3622115344437553
1106	3	1.0039703506085758
1106	4	0.417106931888398
1106	5	0.6526897178680215
1106	6	0.2280806480437363
1106	7	0.12286494270262693
1106	8	0.08659897077637169
1106	9	0.11586042767884663
1106	10-11	0.09350545317952452
1106	12-13	0.04476236842908321
1106	14-15	0.08285139164210165
1106	16-17	0.11081919831192977
1106	18-19	0.08822089721242321
1106	20-21	0.08511543156873103
1106	22-23	0.016213301964207005
1106	24-25	0.019433080019958027
1106	26-27	0.026244772075813216
1106	28-29	0.011916693258562816
1106	30-31	0.043319938910507005
1106	32-33	0.04179322116044659
1106	34-35	-0.036081855975176325
1106	36-37	0.03294775442152087
1106	38-39	-0.002214419700550252
1106	40-41	0.025406173355094097
1106	42-43	0.04356129695323574
1106	44-45	0.1072006471453193
1106	46-47	0.06994172481661565
1106	48-49	0.055236009947542186
1106	50-51	0.10954516670997805
1106	52-53	0.042472113839501446
1106	54-55	-0.04988618180082227
1106	56-57	-0.05521586234327458
1106	58-59	-0.05021598858203902
1106	60-61	-0.04222310422933617
1106	62-63	-0.07019685520228336
1106	64-65	-0.07145637992657328
1106	66-67	-0.06433684975751675
1106	68-69	-0.04871156509445029
1106	70-71	-0.04065390808244729
1106	72-73	-0.029943286039255668
1106	74-75	0.011714712093230162
1106	76-77	-0.06814923609197976
1106	78-79	-0.07388245603165444
1106	80-81	-0.06511130314543223
1106	82-83	-0.09085978668220207
1106	84-85	-0.03876170460869233
1106	86-87	-0.02283153749602107
1106	88-89	0.019304634211987803
1106	90-91	0.043364932330867134
1106	92-93	-0.001146813125487256
1106	94-95	0.04816560273531678
1106	96-97	0.13629665161953497
1106	98-99	0.08344101277609539
1106	100	0.03538239046695324
1107	1	2.8372971956554096
1107	2	1.7988111734370698
1107	3	1.2097082545032762
1107	4	0.5052010859781433
1107	5	0.8095191167541245
1107	6	0.34148470452370816
1107	7	0.16827743143143437
1107	8	0.14951653872791582
1107	9	0.04032807422078122
1107	10-11	0.08729326403041426
1107	12-13	0.08790948275716914
1107	14-15	0.13208003139587277
1107	16-17	0.1863020652765357
1107	18-19	0.16153850425386906
1107	20-21	0.12696880997041404
1107	22-23	0.0942044837437166
1107	24-25	0.1352564232571538
1107	26-27	0.15241450434287884
1107	28-29	0.07974928174872531
1107	30-31	0.18424073588448664
1107	32-33	0.1403945912777047
1107	34-35	0.13247404339626456
1107	36-37	0.16385010687769608
1107	38-39	0.15413665354702033
1107	40-41	0.12278624703461105
1107	42-43	0.0944553961857153
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2216	86-87	-0.2683757252569876
2216	88-89	-0.3202804478858887
2216	90-91	-0.35901554730734375
2216	92-93	-0.35324471608690544
2216	94-95	-0.37693286913948043
2216	96-97	-0.3812376835689193
2216	98-99	-0.4624402778241574
2216	100	-0.51616829121566
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	732.0
3	193.0
4	273.0
5	612.0
6	1794.0
7	3624.0
8	12551.0
9	17205.0
10	4018.0
11	9368.0
12	7370.0
13	10527.0
14	10171.0
15	10725.0
16	13563.0
17	17680.0
18	24189.0
19	40543.0
20	90480.0
21	124479.0
22	57808.0
23	33055.0
24	29983.0
25	31220.0
26	32358.0
27	35077.0
28	40564.0
29	48325.0
30	55632.0
31	68094.0
32	86475.0
33	117698.0
34	174083.0
35	364124.0
36	528477.0
37	1012127.0
38	1432867.0
39	538566.0
40	4036.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.755280107111252	42.45664366359565	25.384251367851334	17.403824861441763
2	16.959686732839522	41.1648748371265	23.75123438128302	18.124204048750965
3	18.13022096370703	24.976334086674505	20.842622413029368	36.0508225365891
4	33.3984417588665	25.838510805359725	22.236593266297128	18.52645416947665
5	17.085885063323765	39.84562721091001	22.92555548745704	20.14293223830919
6	35.88827080778822	23.31331499650537	23.786691171646304	17.011723024060114
7	18.72627275095243	23.216490730289514	39.82734282704856	18.229893691709492
8	35.29318167799656	23.59394625379076	23.263262606503744	17.84960946170894
9	34.99559782551046	25.09156955101749	22.991353194257883	16.921479429214173
10-11	16.923375055444613	42.334067880312716	23.383207619592408	17.359349444650267
12-13	16.80062097965178	24.625982140647213	32.062258651422034	26.511138228278973
14-15	17.06895090291364	33.179509094943214	23.40097062099137	26.35056938115177
16-17	26.10752229816733	24.286768035217403	32.096553200809716	17.509156465805546
18-19	17.329060414596086	24.43686390315765	32.23364410802478	26.000431574221484
20-21	26.194317258893467	33.32596690075225	23.40504211684164	17.07467372351264
22-23	25.870117884547028	24.654360747988694	32.11834559907939	17.35717576838489
24-25	17.183714272356504	33.23233148668564	23.265482355353896	26.318471885603966
26-27	17.50160980901124	28.3381977918017	36.81093986523571	17.349252533951354
28-29	25.63086048073081	24.436350764320423	32.64462842386438	17.28816033108438
30-31	23.262211663464075	25.896699960280245	32.997597956731006	17.843490419524674
32-33	19.502322437017323	27.08644871677387	34.100914703588366	19.31031414262045
34-35	19.552384363930777	26.84841256363138	34.304492215527574	19.294710856910267
36-37	19.592051253038896	26.810628240245283	34.61213507707443	18.985185429641398
38-39	20.16515152214591	26.642663223235264	34.13941438028106	19.05277087433777
40-41	19.030008849531445	27.908947062908286	34.04534574559512	19.015698341965145
42-43	19.206162667752317	26.882673684758483	34.508474110146	19.4026895373432
44-45	19.209180774465537	27.45374828028436	33.80535416835892	19.531716776891187
46-47	19.73399552232737	26.801357641971236	34.21997563740635	19.244671198295052
48-49	19.18272778061964	27.137958997013296	34.15515908281924	19.52415413954782
50-51	19.737703984139465	27.62624806691127	33.42237614358558	19.213671805363685
52-53	19.910744181217776	27.37198413915241	33.17914210733662	19.538129572293194
54-55	19.938465648558804	28.35913470817346	31.442000804415542	20.26039883885219
56-57	20.404341530654158	29.0068138686497	30.198165380549856	20.39067922014628
58-59	21.42301709585305	29.292607846135848	29.03563524328714	20.24873981472396
60-61	20.96944179683602	29.692137270879282	28.818076167831048	20.52034476445365
62-63	20.182045389653805	29.848928040378315	28.821987196732206	21.14703937323568
64-65	20.66333669081071	29.76023093420212	29.12786292063208	20.448569454355088
66-67	20.533583281375414	29.10580518274848	29.7448090207592	20.615802515116908
68-69	20.934954159149626	29.15130191472376	29.439398380090896	20.474345546035718
70-71	19.655503790935544	30.036701237819617	29.56660597921959	20.741188992025243
72-73	19.746200052920415	29.264577128755136	29.841810388941482	21.14741242938297
74-75	19.983919564032785	29.682999722236303	29.204043956596575	21.129036757134344
76-77	20.592446048182467	29.127921287186382	29.481690493884127	20.797942170747024
78-79	20.32098986608332	29.537328203907258	28.974676601786655	21.167005328222764
80-81	20.737473977529945	29.978907004691536	28.564482931862507	20.719136085916016
82-83	20.661032749122388	29.87553371073725	28.70608706481906	20.757346475321302
84-85	20.595903688844814	30.210528042396533	28.055184801726398	21.138383467032256
86-87	20.477882848088186	30.485381353101985	28.254217589838653	20.782518208971172
88-89	21.545912149340307	29.8626886535595	27.86456389371046	20.726835303389734
90-91	20.95086897361623	30.53429656006496	27.70197857383995	20.81285589247886
92-93	20.3372810846618	30.681691381530918	27.66562599944522	21.315401534362056
94-95	20.65066158311419	30.878269276688968	27.53688386392159	20.93418527627525
96-97	20.665953316909395	30.20818825204492	27.88802045835243	21.237837972693256
98-99	21.24953272074488	30.36326087942987	27.12175229867999	21.26545410114526
100	20.60087303921251	31.180005551525163	27.027185775523115	21.191935633739206
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	114679.0
1	63680.0
2	8920.0
3	4221.0
4	3138.0
5	2838.5
6	2954.0
7	3399.5
8	3201.0
9	2886.5
10	2974.0
11	3963.0
12	32717.5
13	36821.0
14	11966.5
15	11406.5
16	13120.0
17	15446.5
18	18010.0
19	20929.0
20	24063.5
21	27683.0
22	31576.0
23	36705.5
24	42034.0
25	47138.0
26	55199.5
27	63756.5
28	69785.0
29	75016.0
30	80865.5
31	87833.5
32	96317.5
33	105181.0
34	113733.5
35	121018.0
36	127564.0
37	133359.5
38	138264.0
39	141934.5
40	144343.0
41	148014.5
42	150582.5
43	153391.0
44	157563.0
45	158809.0
46	160188.5
47	164001.5
48	164907.5
49	170941.0
50	205228.0
51	200844.5
52	153618.5
53	134562.5
54	127924.0
55	124184.5
56	114263.5
57	98205.5
58	87525.5
59	73640.5
60	57658.0
61	46844.5
62	37766.0
63	29654.5
64	22404.0
65	16461.5
66	12251.0
67	9358.5
68	7210.0
69	5498.5
70	4102.5
71	2917.0
72	2185.0
73	1535.0
74	1025.5
75	694.0
76	488.0
77	333.0
78	221.5
79	148.5
80	85.0
81	47.0
82	35.5
83	19.0
84	6.0
85	4.5
86	4.0
87	3.0
88	1.5
89	2.5
90	2.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2911603314772566
2	0.0011589839129104128
3	2.1608174647482273E-4
4	0.0035948145095356875
5	3.928759026814959E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.4732846350556096E-4
16-17	1.9643795134074795E-5
18-19	9.821897567037397E-6
20-21	1.2768466837148617E-4
22-23	0.00264209044553306
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	9.821897567037397E-6
34-35	0.002268858337985639
36-37	3.928759026814959E-5
38-39	1.4732846350556096E-4
40-41	1.9643795134074795E-5
42-43	2.651912343100097E-4
44-45	0.0014045313520863478
46-47	0.0014732846350556097
48-49	0.0021608174647482275
50-51	8.545050883322536E-4
52-53	3.437664148463089E-4
54-55	2.651912343100097E-4
56-57	3.732321075474211E-4
58-59	8.937926786004031E-4
60-61	0.0023179678258208256
62-63	2.455474391759349E-4
64-65	7.562861126618796E-4
66-67	1.5715036107259836E-4
68-69	1.9643795134074795E-4
70-71	0.002003667103675629
72-73	5.893138540222438E-5
74-75	1.5715036107259836E-4
76-77	0.0011589839129104128
78-79	7.464642150948422E-4
80-81	0.002229570747717489
82-83	2.0625984890778535E-4
84-85	0.0012080934007455998
86-87	0.006246726852635784
88-89	0.0023179678258208256
90-91	0.004056443695186446
92-93	0.00671817793585358
94-95	0.0051564962226946336
96-97	0.002534049572295649
98-99	5.303824686200194E-4
100	0.0033983765581949393
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5090666.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.172904722457666
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.02846554676573	54.34668057405063
2	9.946600272761987	12.56711262687129
3	2.0608610730212917	3.905717406365878
4	0.6690903186059302	1.6907351579204508
5	0.2926291093358398	0.9243115421545328
6	0.17493607396366567	0.6630731959816475
7	0.1242368345977342	0.5493881200543677
8	0.08652822099542758	0.4372991248598326
9	0.06852959040572797	0.3896291956833091
>10	0.4755050361937044	6.515568072182636
>50	0.053203790399760774	2.1824507161864988
>100	0.015472857639228647	1.7716238655982992
>500	0.0016758186882988714	0.7292228485471293
>1k	0.00198615375412954	2.1803348630911175
>5k	1.5516826204117618E-4	0.7340059459606774
>10k+	1.2413460963294095E-4	10.412846744491729
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	364251	7.155272021381879	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	110178	2.1643140602820927	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	36068	0.7085124028958097	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	20639	0.40542828777216966	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9411	0.1848677560067779	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7458	0.1465034241099298	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	7045	0.13839053671955692	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	6794	0.13345994414090415	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6732	0.13224202884259154	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0035162393289993885	0.0	0.0	0.0	1.9643795134074795E-5
2	0.0035162393289993885	0.0	0.0	0.0	1.9643795134074795E-5
3	0.003575170714401613	0.0	0.0	0.0	1.9643795134074795E-5
4	0.0036341020998038372	0.0	0.0	0.0	1.9643795134074795E-5
5	0.0036341020998038372	0.0	0.0	0.0	1.9643795134074795E-5
6	0.0036341020998038372	0.0	0.0	0.0	1.9643795134074795E-5
7	0.003653745894937912	0.0	0.0	0.0	1.9643795134074795E-5
8	0.003653745894937912	0.0	0.0	0.0	1.9643795134074795E-5
9	0.003653745894937912	0.0	0.0	0.0	1.9643795134074795E-5
10-11	0.003663567792504949	0.0	0.0	0.0	1.9643795134074795E-5
12-13	0.0036930334852060616	0.0	0.0	0.0	1.9643795134074795E-5
14-15	0.0036930334852060616	0.0	0.0	0.0	1.9643795134074795E-5
16-17	0.0037126772803401363	0.0	0.0	0.0	1.9643795134074795E-5
18-19	0.003830540051144585	0.0	0.0	0.0	1.9643795134074795E-5
20-21	0.003918937129247921	0.0	0.0	0.0	1.9643795134074795E-5
22-23	0.003938580924381997	0.0	0.0	0.0	1.9643795134074795E-5
24-25	0.003997512309784221	0.0	0.0	0.0	1.9643795134074795E-5
26-27	0.004066265592753483	0.0	0.0	0.0	1.9643795134074795E-5
28-29	0.004125196978155707	0.0	0.0	0.0	1.9643795134074795E-5
30-31	0.004174306465990894	0.0	0.0	0.0	1.9643795134074795E-5
32-33	0.004243059748960156	0.0	0.0	0.0	1.9643795134074795E-5
34-35	0.004459141495434979	0.0	0.0	0.0	1.9643795134074795E-5
36-37	0.00479308601271425	0.0	0.0	1.9643795134074795E-5	1.9643795134074795E-5
38-39	0.005451153149705755	0.0	0.0	1.9643795134074795E-5	1.9643795134074795E-5
40-41	0.0063842334185743085	0.0	0.0	3.928759026814959E-5	1.9643795134074795E-5
42-43	0.0077396552828254694	0.0	0.0	3.928759026814959E-5	1.9643795134074795E-5
44-45	0.00928169320085034	0.0	0.0	3.928759026814959E-5	1.9643795134074795E-5
46-47	0.010774621631040024	0.0	0.0	3.928759026814959E-5	1.9643795134074795E-5
48-49	0.012670247861478242	0.0	0.0	3.928759026814959E-5	1.9643795134074795E-5
50-51	0.015165009843505741	0.0	0.0	3.928759026814959E-5	1.9643795134074795E-5
52-53	0.0189366185092481	0.0	0.0	3.928759026814959E-5	1.9643795134074795E-5
54-55	0.023110924975238995	0.0	0.0	5.8931385402224385E-5	1.9643795134074795E-5
56-57	0.028139736529562143	0.0	0.0	5.8931385402224385E-5	1.9643795134074795E-5
58-59	0.03389536850384606	0.0	0.0	5.8931385402224385E-5	1.9643795134074795E-5
60-61	0.04207700917718821	0.0	0.0	5.8931385402224385E-5	1.9643795134074795E-5
62-63	0.05185961915395746	0.0	0.0	5.8931385402224385E-5	1.9643795134074795E-5
64-65	0.0647655925570446	0.0	0.0	5.8931385402224385E-5	1.9643795134074795E-5
66-67	0.08049045056187147	0.0	0.0	5.8931385402224385E-5	1.9643795134074795E-5
68-69	0.1001146019008122	0.0	0.0	5.8931385402224385E-5	1.9643795134074795E-5
70-71	0.12651586256100872	0.0	0.0	5.8931385402224385E-5	1.9643795134074795E-5
72-73	0.1593897537178829	0.0	0.0	5.8931385402224385E-5	1.9643795134074795E-5
74-75	0.1997282870257055	0.0	0.0	5.8931385402224385E-5	1.9643795134074795E-5
76-77	0.2492699383538421	0.0	0.0	5.8931385402224385E-5	1.9643795134074795E-5
78-79	0.30895760986872833	9.821897567037397E-6	0.0	5.8931385402224385E-5	1.9643795134074795E-5
80-81	0.38579431453566193	1.9643795134074795E-5	0.0	5.8931385402224385E-5	1.9643795134074795E-5
82-83	0.47967201148140537	1.9643795134074795E-5	0.0	5.8931385402224385E-5	1.9643795134074795E-5
84-85	0.5955311151821785	1.9643795134074795E-5	0.0	5.8931385402224385E-5	1.9643795134074795E-5
86-87	0.7321046008518335	1.9643795134074795E-5	0.0	5.8931385402224385E-5	1.9643795134074795E-5
88	0.8498102212952097	1.9643795134074795E-5	0.0	5.8931385402224385E-5	1.9643795134074795E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	5070	0.0	87.2961	1
AACGAGT	109825	0.0	71.10168	1
ACGAGTG	117110	0.0	70.53017	2
GTGGAAT	120410	0.0	70.15371	6
CGAGTGG	119335	0.0	69.568924	3
TGGAATC	121560	0.0	69.47456	7
GAATCAC	122040	0.0	69.185905	9
GGAATCA	122280	0.0	69.10008	8
AGTGGAA	121220	0.0	68.83199	5
GAGTGGA	122450	0.0	68.15612	4
AACGTGT	1915	0.0	49.964832	1
CGTGTGG	2180	0.0	45.921616	3
ATCGAGT	840	0.0	44.88982	1
AGTACTT	54675	0.0	41.673443	20-21
GAGTACT	53825	0.0	41.64173	20-21
ACGTGTG	2420	0.0	40.979385	2
GTACTTT	54710	0.0	40.262917	22-23
TACTTTT	58700	0.0	38.286747	22-23
CATGGGG	25250	0.0	38.183754	24-25
ACTTTTT	59730	0.0	36.41801	24-25
>>END_MODULE