FastQCFastQC Report
Thu 26 May 2016
SRR2132653_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132653_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5203561
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4226218.121765075877846No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3242486.231271239061097No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT318770.6125997177701962No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG258400.4965830130558669No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT235260.4521134661436659No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA182160.35006796307374893No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129460.24879116435840765No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC104080.20001687306058294No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT96760.18594958337184864No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA65380.12564472675538924No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54830.10537014940345658No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT52680.1012383634976125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT57150.087.019251
AACGAGT1121150.076.791171
ACGAGTG1208550.075.451782
CGAGTGG1233450.074.3889543
GTGGAAT1257550.074.160176
TGGAATC1268350.073.543527
GGAATCA1277300.073.101798
GAGTGGA1263900.073.0051044
GAATCAC1280300.072.948859
AGTGGAA1271100.072.563615
AACGTGT16750.058.5366171
CGTGTGG21950.047.7478523
ATCTGCG32150.047.07515394
ATCGAGT9800.045.214831
ACGTGTG24050.042.9927522
AGTACTT620750.041.79144320-21
GAGTACT612700.041.23588620-21
GTACTTT619500.040.21629722-23
CATGGGG272900.039.0921124-25
TACTTTT664650.038.75366222-23
Produced by FastQC (version 0.11.5)