##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132653_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5203561
Sequences flagged as poor quality	0
Sequence length	100
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.514759604048074	33.0	30.0	34.0	16.0	34.0
2	31.099953666345026	33.0	31.0	34.0	25.0	34.0
3	31.87327312968946	34.0	31.0	34.0	28.0	34.0
4	35.93335640727571	37.0	35.0	37.0	35.0	37.0
5	35.691858902009606	37.0	35.0	37.0	35.0	37.0
6	36.12483162972433	37.0	36.0	37.0	35.0	37.0
7	36.16089885368885	37.0	35.0	37.0	35.0	37.0
8	36.30887232800769	37.0	37.0	37.0	35.0	37.0
9	38.22375023565593	39.0	39.0	39.0	37.0	39.0
10-11	38.267934593252576	39.0	39.0	39.0	37.0	39.0
12-13	38.224502028514706	39.0	39.0	39.0	37.0	39.0
14-15	39.73930410347837	41.0	40.0	41.0	37.5	41.0
16-17	39.555010789726495	41.0	39.5	41.0	37.0	41.0
18-19	39.17916490265032	41.0	39.0	41.0	35.5	41.0
20-21	39.01008242240266	41.0	39.0	41.0	35.0	41.0
22-23	38.32510409698281	40.5	39.0	41.0	35.0	41.0
24-25	38.28656962030425	41.0	38.0	41.0	35.0	41.0
26-27	38.2107439501526	41.0	38.0	41.0	35.0	41.0
28-29	38.0772954328776	41.0	38.0	41.0	35.0	41.0
30-31	37.92927900720295	40.5	38.0	41.0	34.5	41.0
32-33	37.78738934741036	40.0	38.0	41.0	34.0	41.0
34-35	37.70197303346689	40.0	38.0	41.0	34.0	41.0
36-37	37.54488512770389	40.0	37.5	41.0	34.0	41.0
38-39	37.08162285404168	40.0	36.0	41.0	33.0	41.0
40-41	36.886862765709864	40.0	35.0	41.0	33.0	41.0
42-43	36.757854573051034	40.0	35.0	41.0	33.0	41.0
44-45	36.58683860917552	40.0	35.0	41.0	33.0	41.0
46-47	36.473079108710365	39.5	35.0	41.0	33.0	41.0
48-49	36.31492107039775	39.0	35.0	41.0	32.0	41.0
50-51	36.06710241313593	39.0	35.0	41.0	31.0	41.0
52-53	35.559460627059046	39.0	35.0	41.0	29.0	41.0
54-55	34.66164651476171	39.0	35.0	41.0	22.0	41.0
56-57	33.989518620037316	38.5	35.0	41.0	11.5	41.0
58-59	33.64708763863823	38.0	35.0	41.0	6.0	41.0
60-61	33.414909904967004	37.5	35.0	40.5	2.0	41.0
62-63	33.1619337219262	37.0	34.5	40.0	2.0	41.0
64-65	32.89689858541103	36.5	34.0	40.0	2.0	41.0
66-67	32.60345175159857	36.0	34.0	40.0	2.0	41.0
68-69	32.3232318214392	35.5	34.0	39.0	2.0	41.0
70-71	32.02763348022633	35.0	34.0	39.0	2.0	41.0
72-73	31.70991855385187	35.0	33.0	38.5	2.0	40.5
74-75	31.52454895791555	35.0	33.0	37.5	2.0	40.0
76-77	31.241329447276584	35.0	33.0	37.0	2.0	39.5
78-79	30.932778822041293	35.0	33.0	36.5	2.0	39.0
80-81	30.654271180831742	35.0	33.0	36.0	2.0	39.0
82-83	30.37984824238632	35.0	33.0	36.0	2.0	37.5
84-85	30.09253739890817	35.0	33.0	35.5	2.0	37.0
86-87	29.887732939039246	35.0	33.0	35.0	2.0	37.0
88-89	29.68771779556346	35.0	33.0	35.0	2.0	36.0
90-91	29.517997578965634	35.0	33.0	35.0	2.0	36.0
92-93	29.36840607806846	35.0	32.0	35.0	2.0	36.0
94-95	29.198865065673296	35.0	32.0	35.0	2.0	36.0
96-97	29.02708875710307	35.0	32.0	35.0	2.0	35.0
98-99	28.896028123817516	35.0	32.0	35.0	2.0	35.0
100	28.80465934770439	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.3562528580444386
1101	2	2.052591005742208
1101	3	1.3231199448054056
1101	4	0.5965773956950855
1101	5	0.8475990293042983
1101	6	0.4149193547915999
1101	7	0.2753041815701991
1101	8	0.18852702240743469
1101	9	0.22897211857794275
1101	10-11	0.16660498558273673
1101	12-13	0.21031283927406008
1101	14-15	0.2500466073359533
1101	16-17	0.20197181982664603
1101	18-19	0.11992529404988517
1101	20-21	0.22237119264168825
1101	22-23	-0.12752211101580713
1101	24-25	-0.08490951040958805
1101	26-27	-0.06222538914750686
1101	28-29	-0.04127923406950629
1101	30-31	-0.008042231279084433
1101	32-33	0.03715271657834052
1101	34-35	-0.003706506544773447
1101	36-37	0.059781480688144484
1101	38-39	0.03279633449925967
1101	40-41	0.06144029193523437
1101	42-43	0.14889574211009915
1101	44-45	0.15698476708832487
1101	46-47	0.10256258951182673
1101	48-49	0.09698664050600314
1101	50-51	0.05682326461140974
1101	52-53	0.10122705820481315
1101	54-55	0.11701963183807607
1101	56-57	0.10494621290357742
1101	58-59	0.07593063294343949
1101	60-61	0.06712052097441301
1101	62-63	0.08347603339318255
1101	64-65	0.0866253741206009
1101	66-67	0.004990483482920638
1101	68-69	0.07154173445920264
1101	70-71	0.08745586984476716
1101	72-73	0.08491054132318254
1101	74-75	0.09107416546446956
1101	76-77	0.11588855072050208
1101	78-79	0.005115017152650836
1101	80-81	0.04709353697537466
1101	82-83	0.05801502966040317
1101	84-85	0.10951337637550651
1101	86-87	0.08570643806476852
1101	88-89	0.11539372425908212
1101	90-91	0.1528877724750437
1101	92-93	0.13586593582460083
1101	94-95	0.08321456836288377
1101	96-97	0.061101344828905724
1101	98-99	0.13109884781053793
1101	100	0.16368156119106203
1102	1	3.333372555401521
1102	2	2.00926518845246
1102	3	1.3033508449941564
1102	4	0.6173050528448201
1102	5	0.8865887235403562
1102	6	0.40916459305512376
1102	7	0.2646108352757608
1102	8	0.2159568365511504
1102	9	0.23392364678681332
1102	10-11	0.19507990864073577
1102	12-13	0.19479192931503775
1102	14-15	0.1965244472238652
1102	16-17	0.24452843521027745
1102	18-19	0.14098025104642176
1102	20-21	0.14879251822279116
1102	22-23	0.0503167806702578
1102	24-25	0.05821607296366693
1102	26-27	0.10016020134039394
1102	28-29	0.13781540990591878
1102	30-31	0.17555627601256418
1102	32-33	0.22126422511286137
1102	34-35	0.18467215283621385
1102	36-37	0.1756345916291977
1102	38-39	0.1788683982029724
1102	40-41	0.19421700828515753
1102	42-43	0.1453871764673167
1102	44-45	0.15735947851035093
1102	46-47	0.15133212097202886
1102	48-49	0.189827976215156
1102	50-51	0.28902501744352804
1102	52-53	0.21655602400128515
1102	54-55	0.15639545408713929
1102	56-57	0.09864774511188301
1102	58-59	0.0888497963531023
1102	60-61	0.13289173787247677
1102	62-63	0.1883749434796016
1102	64-65	0.10045661043705678
1102	66-67	0.06807949231630772
1102	68-69	0.10669478149642941
1102	70-71	0.12570608530565863
1102	72-73	0.1244175089340942
1102	74-75	0.07932369374838544
1102	76-77	0.12285543446237668
1102	78-79	0.12609904948676132
1102	80-81	0.0937891210670756
1102	82-83	0.11503362983218679
1102	84-85	0.10975943299772695
1102	86-87	0.19172828258447083
1102	88-89	0.22878265130023223
1102	90-91	0.21182637783010705
1102	92-93	0.17754316465584097
1102	94-95	0.14762650194363047
1102	96-97	0.16669263116311228
1102	98-99	0.199799529228315
1102	100	0.20953411730033622
1103	1	3.324828540995018
1103	2	2.033487145711284
1103	3	1.2968909201218253
1103	4	0.622843503019979
1103	5	0.8749459739237366
1103	6	0.4362815333609831
1103	7	0.2859945773092747
1103	8	0.22766366979303854
1103	9	0.18101638866556868
1103	10-11	0.14046150869138074
1103	12-13	0.18590129354357288
1103	14-15	0.2169481307309482
1103	16-17	0.24808551606397344
1103	18-19	0.07403180284089927
1103	20-21	0.05470532298932085
1103	22-23	-0.024360708461550473
1103	24-25	0.0010962309568824935
1103	26-27	-0.01950847717224491
1103	28-29	-0.026628870401907534
1103	30-31	0.04963351753256262
1103	32-33	0.02661580574849154
1103	34-35	0.07869570409609139
1103	36-37	0.09639951516498257
1103	38-39	0.07129327052812329
1103	40-41	0.07541192872579217
1103	42-43	0.0883458738322247
1103	44-45	0.17316117451057522
1103	46-47	0.1286320442967792
1103	48-49	0.11608627683840211
1103	50-51	0.19920657599104885
1103	52-53	0.1431592928992771
1103	54-55	0.08031948579833426
1103	56-57	0.07756662410568538
1103	58-59	0.06455914791967388
1103	60-61	0.010943254494833354
1103	62-63	0.07086743422932784
1103	64-65	0.021669257632431993
1103	66-67	-0.011003517433401555
1103	68-69	0.04455766997263311
1103	70-71	-0.01637545424892295
1103	72-73	-0.024449174615199354
1103	74-75	0.0336759637578119
1103	76-77	0.06000221832258745
1103	78-79	0.1006911582671357
1103	80-81	0.09088942495430175
1103	82-83	0.056253962138494984
1103	84-85	0.054813545323447954
1103	86-87	0.03457420050678195
1103	88-89	-0.0251202067872498
1103	90-91	-0.030970826688808017
1103	92-93	-0.06876402523147007
1103	94-95	-4.8617901699188337E-4
1103	96-97	0.03422222851254375
1103	98-99	0.06722292432391086
1103	100	0.04696401924043059
1104	1	2.4841347358816
1104	2	1.6761732141489745
1104	3	1.152587220937093
1104	4	0.5112145029645205
1104	5	0.7991401395274167
1104	6	0.34493302246308133
1104	7	0.21652588450244536
1104	8	0.16948386833998086
1104	9	0.16063232788136617
1104	10-11	0.13819132123644096
1104	12-13	0.13048631378648423
1104	14-15	0.10592863652652085
1104	16-17	0.11260959958679706
1104	18-19	0.08306256929866862
1104	20-21	0.05286203920029209
1104	22-23	-0.029314745952532917
1104	24-25	-0.02013713103080761
1104	26-27	-0.009500296816192133
1104	28-29	0.06911193709628805
1104	30-31	0.049471920837980576
1104	32-33	-0.003152509930060887
1104	34-35	0.028212508488522303
1104	36-37	-9.95942657652904E-4
1104	38-39	0.027713740829277356
1104	40-41	0.003975444337797285
1104	42-43	0.02409811996388811
1104	44-45	0.04085327422773588
1104	46-47	0.07789033557291702
1104	48-49	0.015836775978769424
1104	50-51	0.0028292258718138896
1104	52-53	-0.05947457377478571
1104	54-55	-0.0033283290760479645
1104	56-57	0.05287720033550869
1104	58-59	-0.03511876789198709
1104	60-61	-0.06578855461170718
1104	62-63	-0.10630888710614528
1104	64-65	0.016846937738918655
1104	66-67	-0.013665114127981326
1104	68-69	-0.1113242140049664
1104	70-71	-0.14978257254519178
1104	72-73	-0.07468876199249053
1104	74-75	-0.07247358424285721
1104	76-77	0.01041851525564752
1104	78-79	0.06228410929591632
1104	80-81	0.06277839395213647
1104	82-83	0.10822695309850872
1104	84-85	-0.012916267221612543
1104	86-87	-0.031216936978658794
1104	88-89	0.04250852983301456
1104	90-91	0.09165922710743146
1104	92-93	0.052082364319861796
1104	94-95	0.09891755621677234
1104	96-97	0.1289999030730229
1104	98-99	0.10895452545529594
1104	100	0.11742489218147512
1105	1	3.257684632744205
1105	2	1.973164130111492
1105	3	1.2389692160926131
1105	4	0.5911488060042558
1105	5	0.8538645525943451
1105	6	0.3867276226125824
1105	7	0.23655319297117217
1105	8	0.16983866287404936
1105	9	0.16807696527060756
1105	10-11	0.09443173509424696
1105	12-13	0.09567386126530408
1105	14-15	0.15357311469914237
1105	16-17	0.07535934101689179
1105	18-19	0.021926328738175016
1105	20-21	-0.006530417140155009
1105	22-23	-0.08852998240632104
1105	24-25	-0.08849550641848936
1105	26-27	-0.02418935983292414
1105	28-29	-0.021430548510224412
1105	30-31	0.01860696717243826
1105	32-33	0.009104380976481252
1105	34-35	-0.02326051388249084
1105	36-37	-0.05211138308872876
1105	38-39	-0.056278383211839866
1105	40-41	-0.08285213876305164
1105	42-43	-0.01711163175360042
1105	44-45	-0.06772681040939688
1105	46-47	-0.03058756952230368
1105	48-49	-0.11731984933697959
1105	50-51	-0.10909023857325195
1105	52-53	-0.1273281668524575
1105	54-55	-0.12891676734896151
1105	56-57	-0.16159435548835432
1105	58-59	-0.12435673065710517
1105	60-61	-0.07524477835181642
1105	62-63	-0.005665873115596298
1105	64-65	-0.061690089417375304
1105	66-67	-0.07513478755753766
1105	68-69	-0.1006233797563496
1105	70-71	-0.06809586122056288
1105	72-73	-0.0360476639126972
1105	74-75	-0.04585283611798374
1105	76-77	-0.022985207677965747
1105	78-79	-0.1085799682795141
1105	80-81	-0.08343992236552111
1105	82-83	-0.06675893755976858
1105	84-85	-0.05579256667644472
1105	86-87	-0.0416762129450845
1105	88-89	0.0025961972952934786
1105	90-91	0.03884045362818256
1105	92-93	-0.005755184111738032
1105	94-95	-0.004039021540769738
1105	96-97	0.03061884360741729
1105	98-99	0.018650008290080677
1105	100	-0.07858062343590433
1106	1	1.8193211127744533
1106	2	1.373393742344895
1106	3	1.0268293186161657
1106	4	0.4424825368841212
1106	5	0.6573153293437315
1106	6	0.2391936227398972
1106	7	0.1345268863979996
1106	8	0.12283392487533007
1106	9	0.13308581216090687
1106	10-11	0.10892278917511788
1106	12-13	0.060793674445100976
1106	14-15	0.1021713110505047
1106	16-17	0.14021211950511514
1106	18-19	0.1043131308438987
1106	20-21	0.11625972652923622
1106	22-23	-0.023968224112202563
1106	24-25	-0.029159068240900865
1106	26-27	0.010673457502129224
1106	28-29	0.05046718256337357
1106	30-31	0.034240365777954196
1106	32-33	0.015731026592895603
1106	34-35	-0.045547124634374825
1106	36-37	0.037639153698293626
1106	38-39	0.004604768585977581
1106	40-41	-0.051412573723581545
1106	42-43	-0.05134917201851863
1106	44-45	0.038408631696533746
1106	46-47	-0.01586324715909626
1106	48-49	0.010740477487118483
1106	50-51	0.06850813638455833
1106	52-53	0.014091107440698636
1106	54-55	-0.026523270184952707
1106	56-57	-0.06273831335844449
1106	58-59	-0.06660914499114767
1106	60-61	0.037520383183910155
1106	62-63	-0.03642598715700274
1106	64-65	-0.017026515690048427
1106	66-67	-0.06563899736452328
1106	68-69	0.008119762021166821
1106	70-71	0.012068573808797112
1106	72-73	-0.018618922469489263
1106	74-75	-0.0032757961898646215
1106	76-77	-0.03612240959897761
1106	78-79	0.010811725027682684
1106	80-81	0.042588939083270105
1106	82-83	-0.023817984148728044
1106	84-85	-0.028727623570127037
1106	86-87	-0.02668806306490268
1106	88-89	0.042418986586749696
1106	90-91	0.07228007097127076
1106	92-93	0.0892182251255953
1106	94-95	0.07054915008054152
1106	96-97	0.15770209623282128
1106	98-99	0.13084316405588226
1106	100	0.09742466235213953
1107	1	2.8324043481325027
1107	2	1.8340104357574063
1107	3	1.202366207158562
1107	4	0.5240224798280622
1107	5	0.8287909577319184
1107	6	0.3737188105190725
1107	7	0.1481575749213917
1107	8	0.17071787148745443
1107	9	0.08872810892332694
1107	10-11	0.1334259602958383
1107	12-13	0.08211298109059584
1107	14-15	0.14224645267004377
1107	16-17	0.19411185638650608
1107	18-19	0.16984593224458422
1107	20-21	0.14493098619955447
1107	22-23	0.03270763244977104
1107	24-25	0.022571362617611612
1107	26-27	0.08770816605172627
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2216	34-35	0.0212862850758313
2216	36-37	-0.017504752828571668
2216	38-39	-0.003601664879631983
2216	40-41	-0.06123566979781003
2216	42-43	-0.04531266916308141
2216	44-45	-0.011342966167347868
2216	46-47	-0.04660311000041162
2216	48-49	-0.05436665726115564
2216	50-51	-0.01996867572011496
2216	52-53	0.043564279233287095
2216	54-55	0.08204697779730452
2216	56-57	0.19674456229336812
2216	58-59	0.2414855851846056
2216	60-61	0.2591904161739933
2216	62-63	0.16442540956046514
2216	64-65	0.20790436520829303
2216	66-67	0.22584819490336372
2216	68-69	0.2334839845940735
2216	70-71	0.2448803859174049
2216	72-73	0.27015182522127645
2216	74-75	0.2806655916224159
2216	76-77	0.2920336325772652
2216	78-79	0.2841670500605318
2216	80-81	0.33908008302424264
2216	82-83	0.31433383225319744
2216	84-85	0.31711781214896817
2216	86-87	0.32638897379152354
2216	88-89	0.18840669885902273
2216	90-91	0.11779742383096803
2216	92-93	0.1012080295726534
2216	94-95	0.11865359368373518
2216	96-97	0.10707547097288739
2216	98-99	0.0487093050560361
2216	100	0.019142868494057552
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	734.0
3	264.0
4	440.0
5	1442.0
6	4663.0
7	9729.0
8	37729.0
9	54025.0
10	6971.0
11	12973.0
12	9679.0
13	13357.0
14	12870.0
15	13584.0
16	16635.0
17	20593.0
18	27259.0
19	44871.0
20	97706.0
21	130912.0
22	60273.0
23	34114.0
24	31768.0
25	33394.0
26	34057.0
27	35941.0
28	42038.0
29	49614.0
30	57191.0
31	69403.0
32	89416.0
33	124877.0
34	192741.0
35	556583.0
36	538986.0
37	937689.0
38	1295533.0
39	499025.0
40	4482.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.466144396758594	45.96572709739671	25.034781371756488	15.533347134088213
2	15.178336867816727	45.06665231733671	23.643666420357857	16.111344394488704
3	16.247606440836137	27.05684659774446	21.487012323130294	35.20853463828911
4	33.17483437268601	28.076493845657424	22.40702663778042	16.341645143876146
5	15.165702998929387	43.88567281957323	23.150279702903227	17.79834447859416
6	35.32348328385119	25.84136132928969	23.98298011688534	14.852175269973774
7	16.67740610708705	25.648032184113916	41.66027841318666	16.01428329561237
8	34.87873016190259	26.017298538443196	23.419077819977513	15.684893479676706
9	34.36759941893638	27.51329330049172	23.215351948406102	14.9037553321658
10-11	14.90392829064558	46.2998319804457	23.541753426163353	15.25448630274537
12-13	14.754934169119954	26.971010813556333	33.02223227516695	25.251822742156765
14-15	15.023159162695665	36.242081376658575	23.693825914432363	25.040933546213395
16-17	24.88657043318136	26.61477206131687	33.088187038122655	15.410470467379108
18-19	15.310257274802513	26.77028546127214	33.294702732869155	24.624754531056187
20-21	24.929675461201658	36.47633295208745	23.669411681843954	14.924579904866933
22-23	24.66858399171316	27.11595044038259	32.83129589520758	15.384169672696663
24-25	15.2249488379208	36.43133999966561	23.169075946260648	25.17463521615294
26-27	15.642710828219366	30.960211670431075	37.97500404050226	15.422073460847294
28-29	24.127486926741128	26.859087459530116	33.62775030407062	15.385675309658136
30-31	21.682267201249296	28.347481657272777	33.93601996786432	16.03423117361361
32-33	17.8292327119832	29.546833408890567	35.257672582295086	17.36626129683115
34-35	17.68013980720297	29.35652422243198	35.43699792339757	17.526338046967478
36-37	17.73806669763907	29.45913444792337	35.54415416760553	17.258644686832035
38-39	18.10521663095387	29.2326584735421	35.33663556610391	17.32548932940012
40-41	17.208945950667246	30.25528095087191	35.32411746494372	17.211655633517122
42-43	17.383269276060087	29.493327291300268	35.669269026422654	17.45413440621699
44-45	17.434721792354864	29.895770684937006	35.07206765628032	17.597439866427813
46-47	17.846693810029514	29.50211992661837	35.242545280256564	17.408640983095548
48-49	17.266580051214007	29.86640906881278	35.254314941819	17.61269593815422
50-51	17.71723268424194	30.240273646347383	34.536272621653346	17.506221047757332
52-53	18.026947065792772	29.983693777403246	34.21788265092865	17.771476505875334
54-55	18.214125727077004	30.925748447067104	32.40832540764068	18.45180041821521
56-57	18.64790352095326	31.663346487214266	30.96830922984264	18.72044076198983
58-59	19.275473879513864	32.13014903493535	30.03804257951577	18.55633450603501
60-61	19.120149639095423	32.404607094558166	29.61716959872608	18.858073667620328
62-63	18.474593746213525	32.50526107025173	29.684293506079452	19.33585167745529
64-65	18.873991907393368	32.2979466764293	30.098375809428816	18.729685606748514
66-67	18.74874184366374	31.81714624823666	30.45334561726315	18.980766290836453
68-69	18.899560242273203	32.032467468824635	30.302234226000074	18.765738062902095
70-71	17.894856398078627	32.595071994821915	30.458591578745274	19.051480028354188
72-73	17.974244895456966	32.11251316648958	30.54942672801708	19.36381521003638
74-75	18.322168048782807	32.45802460606495	29.892124700529227	19.327682644623017
76-77	18.759387308718946	31.988757954619594	30.020160463103547	19.231694273557913
78-79	18.714181746342433	32.47765409884488	29.383550810825042	19.424613343987644
80-81	19.070526464071634	32.81630786952736	29.073541770644134	19.039623895756876
82-83	18.91173697028701	32.94955039339497	29.134250489425582	19.004462146892436
84-85	18.949311952831703	33.09507359957106	28.618092262988625	19.337522184608613
86-87	18.943265633546318	33.36600512063001	28.586641543984104	19.104087701839575
88-89	19.815001372168126	32.80272642503695	28.41137523532491	18.970896967470004
90-91	19.244172230479204	33.39232106906687	28.224968513021874	19.138538187432058
92-93	18.70456524567656	33.57868220786312	28.339274865885493	19.37747768057483
94-95	18.922678491997424	33.671068141745224	28.165372566433167	19.24088079982419
96-97	18.842602757693587	33.00580336959619	28.5072430415057	19.644350831204534
98-99	19.424952073413987	33.0874138073345	27.778029209585835	19.709604909665675
100	18.8872830981341	33.83292430511647	27.60936620164931	19.67042639510012
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	350166.0
1	194477.5
2	26245.5
3	10844.0
4	7438.5
5	6616.5
6	6603.0
7	7475.5
8	7274.0
9	6440.0
10	6468.0
11	8268.0
12	62241.5
13	67893.5
14	18359.5
15	15868.5
16	17365.0
17	19613.0
18	21877.5
19	24344.5
20	27444.5
21	30727.5
22	34279.0
23	38966.0
24	43842.0
25	49377.5
26	57467.5
27	65510.5
28	71523.0
29	77270.0
30	83741.0
31	90208.0
32	97465.0
33	106053.5
34	115631.5
35	122335.5
36	127031.5
37	132600.0
38	137025.0
39	138842.5
40	140147.5
41	142237.0
42	142733.0
43	142899.0
44	144979.5
45	145537.5
46	143540.5
47	145285.0
48	146899.0
49	150588.5
50	173950.5
51	168791.0
52	134661.0
53	122903.0
54	118979.5
55	118181.5
56	108655.0
57	90996.5
58	80026.5
59	65890.5
60	51096.0
61	41862.0
62	32562.0
63	24044.0
64	17809.5
65	12747.0
66	9123.5
67	6890.5
68	5508.5
69	4389.0
70	3082.5
71	2171.5
72	1722.0
73	1308.5
74	928.0
75	624.0
76	485.5
77	385.0
78	288.5
79	183.0
80	114.0
81	93.5
82	65.5
83	29.5
84	9.5
85	5.5
86	4.0
87	4.0
88	3.0
89	1.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.29670066325733474
2	0.001268362185049815
3	2.4982891523708475E-4
4	0.003113252636031364
5	7.687043545756454E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	9.608804432195567E-5
16-17	2.8826413296586704E-5
18-19	9.608804432195567E-6
20-21	1.4413206648293352E-4
22-23	0.002517506761235239
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0021043281706508293
36-37	6.726163102536898E-5
38-39	2.1139369750830251E-4
40-41	0.0
42-43	2.7865532853367145E-4
44-45	0.0015854527313122686
46-47	0.0015181911002868998
48-49	0.0019505872997357002
50-51	0.0010857949008380992
52-53	4.996578304741695E-4
54-55	3.843521772878227E-4
56-57	5.188754393385607E-4
58-59	9.512716387873612E-4
60-61	0.0020755017573542427
62-63	1.8256728421171578E-4
64-65	8.840100077619923E-4
66-67	2.7865532853367145E-4
68-69	2.690465241014759E-4
70-71	0.0018544992554137445
72-73	1.9217608864391134E-4
74-75	1.2491445761854237E-4
76-77	0.0011434477274312725
78-79	6.533987013892986E-4
80-81	0.002113936975083025
82-83	2.3061130637269363E-4
84-85	0.001268362185049815
86-87	0.005669194614995385
88-89	0.002248460237133763
90-91	0.004218265145733854
92-93	0.006879903973452027
94-95	0.005140710371224629
96-97	0.0022772866504303497
98-99	5.669194614995385E-4
100	0.0032862111158108837
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5203561.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.13358858715618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.56265140401436	38.88494055217868
2	14.389882993585113	14.428329476354232
3	4.533078856650909	6.817785312974192
4	1.4939485328199542	2.9958800445912472
5	0.5928775656589478	1.4861539979650185
6	0.29318146067184414	0.8818943238422248
7	0.18228547176971013	0.6397037392982825
8	0.1325171308426263	0.5314847454731658
9	0.10874318266257738	0.49065173831372666
>10	0.559840968717247	5.009607882744398
>50	0.08680344546352138	3.1612705015120692
>100	0.058000488839042574	4.42020275474306
>500	0.003247051803322347	1.143277289572852
>1k	0.002483039254018346	1.936831821073209
>5k	1.5280241563188173E-4	0.5164157105518011
>10k+	3.0560483126376345E-4	16.655570108811844
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	422621	8.121765075877846	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	324248	6.231271239061097	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31877	0.6125997177701962	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	25840	0.4965830130558669	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23526	0.4521134661436659	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	18216	0.35006796307374893	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12946	0.24879116435840765	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	10408	0.20001687306058294	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9676	0.18594958337184864	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	6538	0.12564472675538924	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5483	0.10537014940345658	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5268	0.1012383634976125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0037666513374206624	0.0	0.0	3.843521772878227E-5	0.0
2	0.003824304164013836	0.0	0.0	3.843521772878227E-5	0.0
3	0.0038819569906070094	0.0	0.0	5.765282659317341E-5	0.0
4	0.0038819569906070094	0.0	0.0	5.765282659317341E-5	0.0
5	0.0038819569906070094	0.0	0.0	7.687043545756454E-5	0.0
6	0.0038819569906070094	0.0	0.0	1.1530565318634682E-4	0.0
7	0.0038819569906070094	0.0	0.0	1.3452326205073793E-4	0.0
8	0.0039011745994714003	0.0	0.0	1.3452326205073793E-4	0.0
9	0.0039011745994714003	0.0	0.0	1.5374087091512908E-4	0.0
10-11	0.003920392208335791	0.0	0.0	1.9217608864391134E-4	0.0
12-13	0.003949218621632378	0.0	0.0	2.0178489307610693E-4	0.0
14-15	0.003997262643793356	0.0	0.0	2.6904652410147587E-4	0.0
16-17	0.004045306665954334	0.0	0.0	3.0748174183025816E-4	0.0
18-19	0.00414139471027629	0.0	0.0	3.3630815512684486E-4	0.0
20-21	0.004285526776759223	0.0	0.0	3.4591695955904045E-4	0.0
22-23	0.004362397212216788	0.0	0.0	4.131785905844094E-4	0.0
24-25	0.004429658843242156	0.0	0.0	4.516138083131917E-4	0.0
26-27	0.004516138083131917	0.0	0.0	4.900490260419739E-4	0.0
28-29	0.00457379090972509	9.608804432195567E-6	0.0	4.996578304741695E-4	0.0
30-31	0.0046890965629114364	1.9217608864391135E-5	0.0	5.765282659317341E-4	0.0
32-33	0.004814011020529979	1.9217608864391135E-5	0.0	5.765282659317341E-4	0.0
34-35	0.004910099064851936	1.9217608864391135E-5	0.0	6.053546792283207E-4	0.0
36-37	0.005140710371224629	1.9217608864391135E-5	0.0	6.149634836605163E-4	0.0
38-39	0.0053905392864617135	1.9217608864391135E-5	0.0	6.149634836605163E-4	0.0
40-41	0.005746065050452949	1.9217608864391135E-5	0.0	7.014427235502764E-4	0.0
42-43	0.006159243641037359	1.9217608864391135E-5	0.0	7.302691368468631E-4	0.0
44-45	0.00678381592913007	1.9217608864391135E-5	0.0	7.302691368468631E-4	0.0
46-47	0.00745643223938376	1.9217608864391135E-5	0.0	7.590955501434499E-4	0.0
48-49	0.008167483767366233	1.9217608864391135E-5	0.0	7.687043545756454E-4	0.0
50-51	0.009186017037178962	1.9217608864391135E-5	0.0	7.975307678722321E-4	0.0
52-53	0.010569684875415125	1.9217608864391135E-5	0.0	8.263571811688188E-4	0.0
54-55	0.012145528802295198	1.9217608864391135E-5	0.0	8.551835944654055E-4	0.0
56-57	0.01439398903942896	1.9217608864391135E-5	0.0	9.3205402992297E-4	0.0
58-59	0.016748146125316873	1.9217608864391135E-5	0.0	9.800980520839478E-4	0.0
60-61	0.0203706653962546	1.9217608864391135E-5	0.0	9.800980520839478E-4	0.0
62-63	0.025117414785759212	1.9217608864391135E-5	0.0	9.800980520839478E-4	0.0
64-65	0.03093074146723753	1.9217608864391135E-5	0.0	9.99315660948339E-4	0.0
66-67	0.039079007625739376	1.9217608864391135E-5	0.0	0.0010185332698127302	0.0
68-69	0.05008108870060329	1.9217608864391135E-5	0.0	0.0010377508786771213	0.0
70-71	0.06490747393948106	1.9217608864391135E-5	0.0	0.0010761860964059035	0.0
72-73	0.08359659856010143	1.9217608864391135E-5	0.0	0.0010761860964059035	0.0
74-75	0.10724386626773473	1.9217608864391135E-5	0.0	0.0010761860964059035	0.0
76-77	0.13717529207402393	1.9217608864391135E-5	0.0	0.0010857949008380992	0.0
78-79	0.17561050980280618	1.9217608864391135E-5	0.0	0.0011146213141346858	0.0
80-81	0.22474993566905432	1.9217608864391135E-5	0.0	0.0011434477274312725	0.0
82-83	0.2866786802345548	1.9217608864391135E-5	0.0	0.0011722741407278591	0.0
84-85	0.3663741041951848	1.9217608864391135E-5	0.0	0.0012299269673210326	1.9217608864391135E-5
86-87	0.4594834191431598	1.9217608864391135E-5	0.0	0.001268362185049815	1.9217608864391135E-5
88	0.5436853723824896	1.9217608864391135E-5	0.0	0.001268362185049815	1.9217608864391135E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	5715	0.0	87.01925	1
AACGAGT	112115	0.0	76.79117	1
ACGAGTG	120855	0.0	75.45178	2
CGAGTGG	123345	0.0	74.388954	3
GTGGAAT	125755	0.0	74.16017	6
TGGAATC	126835	0.0	73.54352	7
GGAATCA	127730	0.0	73.10179	8
GAGTGGA	126390	0.0	73.005104	4
GAATCAC	128030	0.0	72.94885	9
AGTGGAA	127110	0.0	72.56361	5
AACGTGT	1675	0.0	58.536617	1
CGTGTGG	2195	0.0	47.747852	3
ATCTGCG	3215	0.0	47.075153	94
ATCGAGT	980	0.0	45.21483	1
ACGTGTG	2405	0.0	42.992752	2
AGTACTT	62075	0.0	41.791443	20-21
GAGTACT	61270	0.0	41.235886	20-21
GTACTTT	61950	0.0	40.216297	22-23
CATGGGG	27290	0.0	39.09211	24-25
TACTTTT	66465	0.0	38.753662	22-23
>>END_MODULE