FastQCFastQC Report
Thu 26 May 2016
SRR2132652_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132652_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3732916
Sequences flagged as poor quality0
Sequence length100
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3015998.079447809701584No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1711854.585825129737717No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT251770.6744593234886613No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG207150.554928104463106No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT166790.4468088754207167No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103520.2773167143327093No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA93700.25101020221189013No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77110.2065677341788564No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC56210.1505793326182534No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53330.14286418446062005No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43850.11746848844174367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT37750.087.806691
AACGAGT796400.075.326451
ACGAGTG855900.074.075382
GTGGAAT891350.072.697686
CGAGTGG880350.072.49763
TGGAATC901350.071.844217
GGAATCA905200.071.574988
AGTGGAA898350.071.482515
GAATCAC908450.071.3396159
GAGTGGA904600.071.020764
AACGTGT12300.050.984221
CGTGTGG15000.044.8047373
ATCTGCG17300.042.3819594
AGTACTT445800.041.02571520-21
GAGTACT440400.040.6003220-21
GTACTTT442850.039.74902722-23
ACGTGTG16400.039.2610322
TACTTTT471050.038.4569122-23
CATGGGG190450.038.1007824-25
TCGAGTG8850.037.7050972