##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132652_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3732916
Sequences flagged as poor quality	0
Sequence length	100
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.517777255100302	33.0	30.0	34.0	16.0	34.0
2	31.107290118502533	33.0	31.0	34.0	25.0	34.0
3	31.900509681975162	34.0	31.0	34.0	28.0	34.0
4	35.961315497053775	37.0	35.0	37.0	35.0	37.0
5	35.7077560813048	37.0	35.0	37.0	35.0	37.0
6	36.14965726525858	37.0	36.0	37.0	35.0	37.0
7	36.191223161732005	37.0	35.0	37.0	35.0	37.0
8	36.33751817613898	37.0	37.0	37.0	35.0	37.0
9	38.26058850507217	39.0	39.0	39.0	37.0	39.0
10-11	38.29917978331149	39.0	39.0	39.0	37.0	39.0
12-13	38.25981565082097	39.0	39.0	39.0	37.0	39.0
14-15	39.78362130302423	41.0	40.0	41.0	38.0	41.0
16-17	39.60703750097779	41.0	39.5	41.0	37.5	41.0
18-19	39.286083453257454	41.0	39.0	41.0	35.5	41.0
20-21	39.127980779637156	41.0	39.0	41.0	35.0	41.0
22-23	38.397667667850016	40.5	39.0	41.0	35.0	41.0
24-25	38.350514450365345	41.0	39.0	41.0	35.0	41.0
26-27	38.29703682054458	41.0	39.0	41.0	35.0	41.0
28-29	38.1915691379072	41.0	38.5	41.0	35.0	41.0
30-31	38.051965005373816	41.0	38.0	41.0	35.0	41.0
32-33	37.91197699064217	40.5	38.0	41.0	34.5	41.0
34-35	37.821483928381994	40.0	38.0	41.0	34.0	41.0
36-37	37.66144175223873	40.0	38.0	41.0	34.0	41.0
38-39	37.199104131997615	40.0	36.0	41.0	33.5	41.0
40-41	36.999852662101155	40.0	35.0	41.0	33.0	41.0
42-43	36.86592586064085	40.0	35.0	41.0	33.0	41.0
44-45	36.69284079791777	40.0	35.0	41.0	33.0	41.0
46-47	36.5722054822557	40.0	35.0	41.0	33.0	41.0
48-49	36.41067251446323	39.5	35.0	41.0	32.5	41.0
50-51	36.16089847722263	39.0	35.0	41.0	31.5	41.0
52-53	35.66331562242493	39.0	35.0	41.0	30.0	41.0
54-55	34.8176629208908	39.0	35.0	41.0	24.0	41.0
56-57	34.18942912189827	39.0	35.0	41.0	16.0	41.0
58-59	33.86638809981258	38.0	35.0	41.0	9.0	41.0
60-61	33.637013664384625	37.5	35.0	40.5	7.0	41.0
62-63	33.37742893223421	37.0	35.0	40.0	6.0	41.0
64-65	33.10351867012277	36.5	34.0	40.0	2.0	41.0
66-67	32.80048573286942	36.0	34.0	39.5	2.0	41.0
68-69	32.50927840326436	35.5	34.0	39.0	2.0	41.0
70-71	32.20819796105779	35.0	34.0	39.0	2.0	41.0
72-73	31.89091825264753	35.0	33.5	38.5	2.0	40.5
74-75	31.70850589726637	35.0	34.0	37.0	2.0	40.0
76-77	31.42369115726151	35.0	34.0	37.0	2.0	39.0
78-79	31.114602900252777	35.0	33.0	36.5	2.0	39.0
80-81	30.83463946148266	35.0	33.0	36.0	2.0	39.0
82-83	30.5582429393	35.0	33.0	36.0	2.0	37.0
84-85	30.276440187778135	35.0	33.0	35.0	2.0	37.0
86-87	30.07702570858814	35.0	33.0	35.0	2.0	37.0
88-89	29.883521354351398	35.0	33.0	35.0	2.0	36.0
90-91	29.711827027449853	35.0	33.0	35.0	2.0	36.0
92-93	29.564973870293358	35.0	33.0	35.0	2.0	36.0
94-95	29.398529728501792	35.0	33.0	35.0	2.0	35.5
96-97	29.22362102442166	35.0	32.0	35.0	2.0	35.0
98-99	29.09376423150159	35.0	32.0	35.0	2.0	35.0
100	29.004056078411622	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.386357353027101
1101	2	2.0527552081816047
1101	3	1.3207110151637096
1101	4	0.5862446667967873
1101	5	0.8563545534738566
1101	6	0.4136950399264592
1101	7	0.271875073925564
1101	8	0.17574374911124835
1101	9	0.2082688617570767
1101	10-11	0.1414110337574428
1101	12-13	0.16093665386420497
1101	14-15	0.21679470480540175
1101	16-17	0.17037667913970722
1101	18-19	0.11065323995008214
1101	20-21	0.18116572986445334
1101	22-23	-0.19307307786765904
1101	24-25	-0.16392487775079445
1101	26-27	-0.11567756474492796
1101	28-29	-0.09464056975806301
1101	30-31	-0.054491269184566704
1101	32-33	-0.07507757837243645
1101	34-35	-0.09061874427266758
1101	36-37	-0.08266460425576838
1101	38-39	-0.05084400737951711
1101	40-41	-0.0682595961302539
1101	42-43	0.0053416728344473086
1101	44-45	-0.015928732304743676
1101	46-47	-0.047941928532544864
1101	48-49	-0.04573494877345752
1101	50-51	-0.1099580879568407
1101	52-53	-0.1434734692368238
1101	54-55	-0.09521936706055811
1101	56-57	-0.18689516378382365
1101	58-59	-0.26389840056237546
1101	60-61	-0.200955297822631
1101	62-63	-0.16204803875904616
1101	64-65	-0.11602448809409083
1101	66-67	-0.06868740485107594
1101	68-69	-0.09187992334129547
1101	70-71	-0.12705114655783234
1101	72-73	-0.07121744956651455
1101	74-75	-0.021914823308463838
1101	76-77	0.005463233611333607
1101	78-79	-0.1740905293339985
1101	80-81	-0.09808961148237927
1101	82-83	-0.11409937004529169
1101	84-85	-0.06882931337013787
1101	86-87	-0.1261222252913612
1101	88-89	-0.02770858151231792
1101	90-91	-0.02843208981884615
1101	92-93	-0.00872306791124089
1101	94-95	-0.035662193460296976
1101	96-97	-0.012623345518846918
1101	98-99	0.03993960218343062
1101	100	0.10388649146036144
1102	1	3.2749121731306445
1102	2	1.9912983734291068
1102	3	1.3016805684517152
1102	4	0.6167125884049511
1102	5	0.814939133793338
1102	6	0.38830908060663205
1102	7	0.20251630240858276
1102	8	0.18037293832269086
1102	9	0.15273597614823586
1102	10-11	0.1589224897299033
1102	12-13	0.12525429681282674
1102	14-15	0.17079578350544722
1102	16-17	0.21189720809771728
1102	18-19	0.11903260162000606
1102	20-21	0.10659839901006762
1102	22-23	-0.003341444140566807
1102	24-25	0.010841079388264063
1102	26-27	0.03170111487948901
1102	28-29	0.030174419833230104
1102	30-31	0.014597027615380398
1102	32-33	0.08333162974268049
1102	34-35	0.08485363204955831
1102	36-37	0.07612592488025882
1102	38-39	0.04672274935498422
1102	40-41	0.05663035727257437
1102	42-43	0.015037942835462559
1102	44-45	0.0650267252832677
1102	46-47	0.08958725033139103
1102	48-49	0.12588624865590958
1102	50-51	0.16031193892442985
1102	52-53	0.1262866229227697
1102	54-55	-0.030470209325571318
1102	56-57	-0.11899735211517282
1102	58-59	-0.2038644874347213
1102	60-61	-0.13714527392322395
1102	62-63	-0.07970599404667666
1102	64-65	-0.09098629688323712
1102	66-67	-0.04876330327635969
1102	68-69	-0.012000697134730842
1102	70-71	0.007374996424722724
1102	72-73	-0.052662967425977314
1102	74-75	-0.013454308792468339
1102	76-77	-0.027068591934106934
1102	78-79	-0.038615275458415255
1102	80-81	-0.07215435796283742
1102	82-83	-0.07064250775506409
1102	84-85	-0.04819016968773937
1102	86-87	0.011709277478544777
1102	88-89	-0.011550333601569207
1102	90-91	0.04444577368749236
1102	92-93	-0.034561070140963324
1102	94-95	-0.014411621843848366
1102	96-97	-0.04907373393987058
1102	98-99	0.005231635602548579
1102	100	0.02288412933535966
1103	1	3.3721448646766845
1103	2	2.0410392101032535
1103	3	1.3214115814733773
1103	4	0.5980329177385144
1103	5	0.9112948456597678
1103	6	0.4294685508596672
1103	7	0.26616932578058083
1103	8	0.2267713524636079
1103	9	0.19203180785749652
1103	10-11	0.1539966656018379
1103	12-13	0.17617765801137608
1103	14-15	0.2098398627389244
1103	16-17	0.25230840241188446
1103	18-19	0.15298986360029687
1103	20-21	0.20260992349278695
1103	22-23	0.09230445978871415
1103	24-25	0.11356410076984247
1103	26-27	0.13605316188333916
1103	28-29	0.13544697609756895
1103	30-31	0.207345627774572
1103	32-33	0.15833529676952196
1103	34-35	0.26013309928937645
1103	36-37	0.2645757607559318
1103	38-39	0.25465823383852637
1103	40-41	0.28708300975998213
1103	42-43	0.2744292666464645
1103	44-45	0.316170520000135
1103	46-47	0.24613857792076743
1103	48-49	0.30422296207689925
1103	50-51	0.3051775288640499
1103	52-53	0.19204575193460016
1103	54-55	0.16613266119721715
1103	56-57	0.26756993585510713
1103	58-59	0.26456986419758977
1103	60-61	0.16931341280432832
1103	62-63	0.24436901283183232
1103	64-65	0.24871136137890204
1103	66-67	0.23813093129174234
1103	68-69	0.2312110095927693
1103	70-71	0.2268136177259592
1103	72-73	0.2162147855232952
1103	74-75	0.26057981448931145
1103	76-77	0.26820716394641764
1103	78-79	0.28898868187268434
1103	80-81	0.3130561300760206
1103	82-83	0.2803656680566924
1103	84-85	0.32082151962792693
1103	86-87	0.34126908136294887
1103	88-89	0.3667861110321198
1103	90-91	0.36650508520241587
1103	92-93	0.29056882219820324
1103	94-95	0.34447476231967755
1103	96-97	0.354010865713299
1103	98-99	0.39870276048022646
1103	100	0.33273051354915495
1104	1	2.484994069213492
1104	2	1.7441345013820886
1104	3	1.1882122084099365
1104	4	0.5008045080950438
1104	5	0.8198399759824611
1104	6	0.3463251037651318
1104	7	0.19939779532245439
1104	8	0.18097538803493762
1104	9	0.15261138308635225
1104	10-11	0.13243620628096409
1104	12-13	0.10847359477863705
1104	14-15	0.05500916098343822
1104	16-17	0.10665546620492705
1104	18-19	0.10824872450749012
1104	20-21	0.053144703871573995
1104	22-23	-0.08077804276633316
1104	24-25	-0.04907741439339475
1104	26-27	-0.0011936685971249972
1104	28-29	-0.026961756839185114
1104	30-31	0.014533674633426585
1104	32-33	-0.06175361699384041
1104	34-35	-0.012589763304944768
1104	36-37	0.01921003528443066
1104	38-39	0.031219637391082244
1104	40-41	-0.037864755925809845
1104	42-43	-0.016239925320000737
1104	44-45	0.04979116805920114
1104	46-47	0.08957597016085117
1104	48-49	0.02487477518891268
1104	50-51	0.03523822590668857
1104	52-53	0.08352857419733795
1104	54-55	0.12293400982625968
1104	56-57	0.07953061630090019
1104	58-59	0.014761882236989265
1104	60-61	-0.00611869797775455
1104	62-63	-0.10766957088720375
1104	64-65	0.03220652437533289
1104	66-67	0.13870624707899992
1104	68-69	0.03174908646321484
1104	70-71	-0.03682122292558887
1104	72-73	0.02381031826754665
1104	74-75	0.07079605810274714
1104	76-77	0.07082764603700653
1104	78-79	0.14312776511939163
1104	80-81	0.15017023777084404
1104	82-83	0.22089394280398622
1104	84-85	0.09921517979852723
1104	86-87	0.07962139084133213
1104	88-89	0.14098163187988177
1104	90-91	0.17829719741540728
1104	92-93	0.10718899481373256
1104	94-95	0.17302787077612436
1104	96-97	0.18875373520425853
1104	98-99	0.17704359359206023
1104	100	0.1580200952628381
1105	1	3.328987314887833
1105	2	2.008406596525301
1105	3	1.278046342691347
1105	4	0.6102590994650683
1105	5	0.8832211524558389
1105	6	0.41192932181458985
1105	7	0.2235970352629124
1105	8	0.16550553214226227
1105	9	0.1932867602365107
1105	10-11	0.11340745527309792
1105	12-13	0.11049036303329984
1105	14-15	0.17338877197639846
1105	16-17	0.10637997331608062
1105	18-19	0.15422560804168484
1105	20-21	0.0858185167287786
1105	22-23	0.09894559155485894
1105	24-25	0.09524869873047948
1105	26-27	0.1059955776012913
1105	28-29	0.11090536415324692
1105	30-31	0.1538855281345377
1105	32-33	0.1302827880456192
1105	34-35	0.20449581286112561
1105	36-37	0.14999291205558762
1105	38-39	0.20203304597830396
1105	40-41	0.1885306294099749
1105	42-43	0.21477397361290684
1105	44-45	0.21772451817543015
1105	46-47	0.2317431155755756
1105	48-49	0.17155262187949205
1105	50-51	0.18708200956402976
1105	52-53	0.16803958025698762
1105	54-55	0.3018040515974789
1105	56-57	0.13448239317065713
1105	58-59	0.14659547146996488
1105	60-61	0.0671270832405142
1105	62-63	0.10294365411726147
1105	64-65	0.1501782396470972
1105	66-67	0.137205483734121
1105	68-69	0.17484265588128522
1105	70-71	0.229983991727309
1105	72-73	0.20573794517735067
1105	74-75	0.23434121419757048
1105	76-77	0.22156228384499954
1105	78-79	0.21797653100228942
1105	80-81	0.27815241860580286
1105	82-83	0.28409591036152904
1105	84-85	0.25367411730636746
1105	86-87	0.2798310484503759
1105	88-89	0.2832262101917067
1105	90-91	0.2684806950747074
1105	92-93	0.3218465919853415
1105	94-95	0.33128863983669277
1105	96-97	0.3591611777079855
1105	98-99	0.36169849125001363
1105	100	0.2933523155415685
1106	1	1.8813350940444487
1106	2	1.4399150728167136
1106	3	1.0069399421104563
1106	4	0.4542985329942226
1106	5	0.6495055915506356
1106	6	0.26003531584593986
1106	7	0.16241257362148076
1106	8	0.13059872785236593
1106	9	0.15151690277739505
1106	10-11	0.08390580688340066
1106	12-13	0.019076724628042996
1106	14-15	0.06864350455024493
1106	16-17	0.06284810533638563
1106	18-19	0.058461179527824925
1106	20-21	0.07672885036569
1106	22-23	-0.12407629643276152
1106	24-25	-0.08179740824708404
1106	26-27	-0.08797825765205403
1106	28-29	-0.05477031584077707
1106	30-31	0.021056032167187766
1106	32-33	-0.02659207103791772
1106	34-35	-0.04102282184258854
1106	36-37	0.013356585701060908
1106	38-39	-0.0334310329739651
1106	40-41	-0.05514681525756515
1106	42-43	-0.04687952901547021
1106	44-45	-0.003651643109336078
1106	46-47	0.002012627733897432
1106	48-49	0.04209287117070204
1106	50-51	0.07046443064636776
1106	52-53	0.0287881994976118
1106	54-55	0.14049780262939038
1106	56-57	0.1870313167020612
1106	58-59	0.17993679376787242
1106	60-61	0.2311852642065375
1106	62-63	0.17322133277269813
1106	64-65	0.1485568349030757
1106	66-67	0.1526046567875241
1106	68-69	0.18256958242009347
1106	70-71	0.19422964706207324
1106	72-73	0.19148066146080467
1106	74-75	0.19734982275073776
1106	76-77	0.19943072157634845
1106	78-79	0.23716975209955038
1106	80-81	0.2620342387404797
1106	82-83	0.24253607390451037
1106	84-85	0.21167240234197493
1106	86-87	0.23452527526950462
1106	88-89	0.24250343968980204
1106	90-91	0.19272027879639353
1106	92-93	0.19055559306296388
1106	94-95	0.2750933753398286
1106	96-97	0.2926683154040006
1106	98-99	0.279191838217848
1106	100	0.32175219694982715
1107	1	2.8631684485827122
1107	2	1.8285559210982107
1107	3	1.2191392832034822
1107	4	0.5188119904857089
1107	5	0.8356053253168128
1107	6	0.35914779074575875
1107	7	0.16004689368210734
1107	8	0.12116888232171874
1107	9	0.0657244956174523
1107	10-11	0.0797977138967596
1107	12-13	0.07818711327885097
1107	14-15	0.12327683443283632
1107	16-17	0.18274444088741149
1107	18-19	0.16386424836851177
1107	20-21	0.15286445339043553
1107	22-23	-0.036335617184853675
1107	24-25	0.026416011865777023
1107	26-27	0.043140675136747575
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2216	40-41	0.05050579192862159
2216	42-43	-0.016269690081259114
2216	44-45	-0.0065691786517021455
2216	46-47	-0.03960486476069747
2216	48-49	-0.0154814421612528
2216	50-51	-0.0013525491882617757
2216	52-53	0.053370929509746645
2216	54-55	0.09033462637386691
2216	56-57	0.2083128547937605
2216	58-59	0.27419194133232594
2216	60-61	0.33842029671600216
2216	62-63	0.35594526510381996
2216	64-65	0.34730879860875774
2216	66-67	0.276984587016571
2216	68-69	0.25808930054097345
2216	70-71	0.23873665211040418
2216	72-73	0.25043669681589975
2216	74-75	0.25069804577179866
2216	76-77	0.22800592381706863
2216	78-79	0.3387477748086489
2216	80-81	0.34210632980051514
2216	82-83	0.2966728540421535
2216	84-85	0.24681183243404803
2216	86-87	0.2390687032147838
2216	88-89	0.17717409619644542
2216	90-91	0.1961506839487086
2216	92-93	0.2860649266050146
2216	94-95	0.3140853024828516
2216	96-97	0.20611173272710204
2216	98-99	0.09754340163505759
2216	100	0.06614284719497832
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	431.0
3	138.0
4	295.0
5	948.0
6	3442.0
7	7354.0
8	29362.0
9	42047.0
10	4944.0
11	8315.0
12	6487.0
13	8677.0
14	8748.0
15	9180.0
16	11341.0
17	14100.0
18	18569.0
19	29363.0
20	61981.0
21	81961.0
22	39202.0
23	23729.0
24	22727.0
25	23710.0
26	24014.0
27	25400.0
28	29309.0
29	34318.0
30	40178.0
31	48589.0
32	61916.0
33	87047.0
34	133810.0
35	360815.0
36	428366.0
37	720667.0
38	943153.0
39	335677.0
40	2606.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.727127154666912	43.70567378268389	26.364549611913446	17.202649450735752
2	15.558014791311878	42.17554496316102	25.50597168342724	16.760468562099856
3	16.81593351249641	26.090355197229403	21.12294637537665	35.97076491489754
4	32.79952250104011	26.85202970545033	23.208340800951998	17.140106992557563
5	15.676082719246832	41.30208126837036	24.14123435941232	18.880601652970494
6	35.845087325833205	23.781622731398187	24.964880002657438	15.408409940111161
7	17.620942983983568	23.876374394709124	41.68127544257626	16.82140717873105
8	34.83140258178861	24.231539097049062	24.43944626667195	16.497612054490375
9	34.4885338968249	25.890804936408962	24.30836911411883	15.312292052647313
10-11	15.407378574819255	44.18230948673905	24.418577862453912	15.991734075987779
12-13	15.308863097910589	25.388007659427643	33.498021385962076	25.805107856699692
14-15	15.702686564285386	34.40773670078751	24.319545426904327	25.570031308022777
16-17	25.298553543276505	24.89640133782848	33.61339596535147	16.191649153543544
18-19	15.95818335561038	25.22176031040617	33.740115467387355	25.079940866596097
20-21	25.404861810874337	34.587940050515556	24.474515120176367	15.532683018433744
22-23	25.090528096861913	25.486341555805414	33.26590440093666	16.157225946396007
24-25	15.817527102136776	34.62514291776188	23.867574303841824	25.689755676259523
26-27	16.330691609454913	29.14693231779124	38.40438681181146	16.117989260942384
28-29	24.52218319404991	25.19111064915471	34.205216511702915	16.08148964509247
30-31	22.148274967880337	26.694680512500145	34.473344698889555	16.68369982072996
32-33	18.46543024273785	27.717433234500856	35.64481493824131	18.172321584519985
34-35	18.317971023111912	27.613863128767747	35.75252549200514	18.3156403561152
36-37	18.235670814319484	27.760426872889806	35.98086696065501	18.02303535213571
38-39	18.767330662135436	27.513650889239944	35.743028154733025	17.9759902938916
40-41	17.820095019575856	28.488848527223364	35.76463701959461	17.926419433606178
42-43	17.935400497413813	27.834438995014075	36.07262756006845	18.15753294750366
44-45	18.016087392485282	28.155227317599017	35.44490405616496	18.383781233750735
46-47	18.392651967136217	27.771070087490486	35.66357779538119	18.17270014999211
48-49	17.90023766077397	28.131746284968166	35.660983730490564	18.3070323237673
50-51	18.314618540771242	28.534419573540614	34.908137854029974	18.242824031658163
52-53	18.576233527726355	28.311475671136783	34.738286022280526	18.37400477885634
54-55	18.777426695830727	29.214271458823625	32.99132980882182	19.016972036523832
56-57	19.068033396001287	29.928578355392133	31.79974989967591	19.20363834893067
58-59	19.776582961514077	30.270384738665236	30.75578684526671	19.19724545455398
60-61	19.502944338783205	30.65925783347619	30.410640258901967	19.427157568838645
62-63	18.887191969370797	30.682098246193046	30.474619251264695	19.95609053317146
64-65	19.187692629159482	30.630584655032234	30.91477503325176	19.26694768255652
66-67	19.106463738271714	30.114244666258315	31.291396161626743	19.487895433843224
68-69	19.401710409150187	30.138071980343472	31.14610646712965	19.314111143376692
70-71	18.3668816813121	30.786813762633958	31.410292386788473	19.436012169265464
72-73	18.48435691131625	30.213207629810572	31.477944622567293	19.82449083630588
74-75	18.77927424190856	30.60076045219943	30.783874941550298	19.83609036434171
76-77	19.226836912635804	30.24821226864505	30.796436043171564	19.72851477554758
78-79	19.102784355190465	30.67797550050323	30.189654511929486	20.02958563237682
80-81	19.54893854932686	31.05709473980866	29.874375793548047	19.519590917316428
82-83	19.29740012274616	31.17866317055314	29.91932292982386	19.604613776876842
84-85	19.34877185650719	31.383533313509442	29.266958131410952	20.000736698572414
86-87	19.313974534278636	31.50426607628143	29.437413199653655	19.744346189786278
88-89	20.16755665344255	31.14044663736091	29.127401296658	19.56459541253854
90-91	19.69669388376735	31.65524684325701	28.97052009858112	19.67753917439452
92-93	19.10733627474803	31.794511222660667	28.98361521816415	20.114537284427154
94-95	19.347836133229656	31.94513931166955	28.87604305430814	19.830981500792653
96-97	19.36766861237177	31.373764779978337	29.11561635292406	20.14295025472583
98-99	19.965919221966004	31.49190333724808	28.35666999562806	20.18550744515786
100	19.38037950589919	32.20310967554927	28.28522066941152	20.131290149140018
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	186398.0
1	106424.5
2	18100.5
3	7827.5
4	5500.5
5	4934.5
6	5025.0
7	5873.5
8	5783.0
9	5162.0
10	5127.5
11	6696.0
12	57711.0
13	62621.5
14	15091.0
15	12157.0
16	13076.0
17	14697.0
18	16052.5
19	17478.0
20	19407.5
21	21653.5
22	24336.5
23	27435.0
24	30382.5
25	33953.5
26	38845.5
27	43829.5
28	48367.5
29	52243.5
30	56449.5
31	60514.5
32	64501.0
33	70063.0
34	76057.0
35	81046.5
36	86032.5
37	89789.0
38	92229.5
39	94561.0
40	96010.0
41	97995.0
42	98646.0
43	99162.0
44	101321.0
45	103444.0
46	105770.5
47	108905.0
48	109988.5
49	112460.5
50	132241.0
51	130267.0
52	103040.5
53	92245.0
54	88358.0
55	88224.5
56	82781.5
57	71631.0
58	64549.0
59	55372.0
60	44827.5
61	36619.0
62	29584.0
63	23075.5
64	17569.0
65	13323.5
66	9766.0
67	7141.0
68	5235.5
69	3643.0
70	2571.0
71	1894.0
72	1394.0
73	958.5
74	743.5
75	632.5
76	441.0
77	273.5
78	200.5
79	134.5
80	90.0
81	65.5
82	38.5
83	23.0
84	11.0
85	4.0
86	2.5
87	1.0
88	0.0
89	1.5
90	1.5
91	1.0
92	1.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.29298810902790207
2	0.0013126467351528939
3	1.0715483552268522E-4
4	0.003562898281129283
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.8752096216469912E-4
16-17	2.6788708880671304E-5
18-19	1.3394354440335652E-5
20-21	1.7412660772436346E-4
22-23	0.0025985047614251163
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.002116308001573033
36-37	4.018306332100695E-5
38-39	2.4109837992604175E-4
40-41	2.6788708880671304E-5
42-43	2.1430967104537043E-4
44-45	0.0014733789884369217
46-47	0.001419801570675579
48-49	0.001995758811610012
50-51	0.0010715483552268521
52-53	3.884362787697339E-4
54-55	2.9467579768738435E-4
56-57	3.7504192432939824E-4
58-59	8.43844329741146E-4
60-61	0.001995758811610012
62-63	2.1430967104537043E-4
64-65	8.304499753008104E-4
66-67	1.7412660772436346E-4
68-69	2.1430967104537043E-4
70-71	0.0018618152672066554
72-73	1.4733789884369217E-4
74-75	1.4733789884369217E-4
76-77	0.0011385201274285305
78-79	6.1614030425544E-4
80-81	0.002049336229371355
82-83	2.4109837992604175E-4
84-85	0.00123228060851088
86-87	0.005679206282702316
88-89	0.0023841950903797458
90-91	0.0040852781043023735
92-93	0.006563233675764469
94-95	0.00504967162400654
96-97	0.002116308001573033
98-99	5.357741776134261E-4
100	0.0030807015212771997
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3732916.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.90300037958883
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.2886572033878	44.62991177293867
2	12.354037213218767	13.56547419613609
3	3.339223591992639	5.500001824161115
4	1.1169335050139597	2.4529200259902764
5	0.5125690968533593	1.4070790659552745
6	0.3034976005958812	0.9997757328431978
7	0.18697558976126985	0.718586461294579
8	0.13456386660325376	0.5910368015357905
9	0.09966058094890749	0.49244984223011207
>10	0.5425738210216757	5.653782485970634
>50	0.09393961143071565	3.478323934105826
>100	0.02190797143967273	2.2928530613606353
>500	0.0029251862867815466	1.1440715789416147
>1k	0.002047630398072457	1.7164285697651505
>5k	1.9501241886404352E-4	0.7504105255885534
>10k+	2.9251862829606526E-4	14.606894121182549
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	301599	8.079447809701584	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	171185	4.585825129737717	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	25177	0.6744593234886613	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	20715	0.554928104463106	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16679	0.4468088754207167	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10352	0.2773167143327093	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	9370	0.25101020221189013	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7711	0.2065677341788564	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	5621	0.1505793326182534	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5333	0.14286418446062005	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4385	0.11746848844174367	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003857574078816668	0.0	0.0	0.0	0.0
2	0.00391115149657801	0.0	0.0	0.0	0.0
3	0.003991517623220024	0.0	0.0	0.0	0.0
4	0.004018306332100695	0.0	0.0	0.0	0.0
5	0.004018306332100695	0.0	0.0	0.0	0.0
6	0.004125461167623381	0.0	0.0	0.0	0.0
7	0.004152249876504052	0.0	0.0	0.0	0.0
8	0.004179038585384724	0.0	0.0	0.0	0.0
9	0.004232616003146066	0.0	0.0	0.0	0.0
10-11	0.004246010357586401	0.0	0.0	0.0	0.0
12-13	0.004299587775347744	0.0	0.0	0.0	0.0
14-15	0.004379953901989758	0.0	0.0	0.0	0.0
16-17	0.0045138974463931145	0.0	0.0	0.0	0.0
18-19	0.004647840990796471	0.0	0.0	0.0	0.0
20-21	0.0046880240541174786	0.0	0.0	0.0	0.0
22-23	0.0047416014718788205	0.0	0.0	0.0	0.0
24-25	0.004795178889640163	0.0	0.0	0.0	0.0
26-27	0.0049425167884838556	0.0	0.0	0.0	0.0
28-29	0.005103249041767883	0.0	0.0	0.0	0.0
30-31	0.0051970095228502326	0.0	0.0	0.0	2.6788708880671304E-5
32-33	0.005330953067253589	0.0	0.0	0.0	2.6788708880671304E-5
34-35	0.0054247135483359386	0.0	0.0	0.0	2.6788708880671304E-5
36-37	0.005639023219381309	0.0	0.0	0.0	2.6788708880671304E-5
38-39	0.006067642561472051	0.0	0.0	0.0	2.6788708880671304E-5
40-41	0.006723965929048497	0.0	0.0	0.0	2.6788708880671304E-5
42-43	0.00760799332211065	0.0	0.0	0.0	2.6788708880671304E-5
44-45	0.008451837651851797	0.0	0.0	0.0	2.6788708880671304E-5
46-47	0.009657329551482005	0.0	0.0	0.0	2.6788708880671304E-5
48-49	0.01087621580555255	6.697177220167826E-5	0.0	0.0	2.6788708880671304E-5
50-51	0.012644270591676856	3.348588610083913E-4	0.0	0.0	2.6788708880671304E-5
52-53	0.01429177618783814	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
54-55	0.016555422088254865	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
56-57	0.020011165533861463	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
58-59	0.024069654929283167	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
60-61	0.0296417063764628	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
62-63	0.036780897293161696	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
64-65	0.04619712846471766	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
66-67	0.05830562487878109	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
68-69	0.07346803410524105	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
70-71	0.09262196095492103	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
72-73	0.11761582634058736	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
74-75	0.14906577056649545	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
76-77	0.19109725480026873	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
78-79	0.2430003782565694	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
80-81	0.3083782222798477	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
82-83	0.3917446843164968	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
84-85	0.4925505958344629	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
86-87	0.6116398011634872	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
88	0.7148299077718331	5.625628864940974E-4	0.0	0.0	2.6788708880671304E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	3775	0.0	87.80669	1
AACGAGT	79640	0.0	75.32645	1
ACGAGTG	85590	0.0	74.07538	2
GTGGAAT	89135	0.0	72.69768	6
CGAGTGG	88035	0.0	72.4976	3
TGGAATC	90135	0.0	71.84421	7
GGAATCA	90520	0.0	71.57498	8
AGTGGAA	89835	0.0	71.48251	5
GAATCAC	90845	0.0	71.339615	9
GAGTGGA	90460	0.0	71.02076	4
AACGTGT	1230	0.0	50.98422	1
CGTGTGG	1500	0.0	44.804737	3
ATCTGCG	1730	0.0	42.38195	94
AGTACTT	44580	0.0	41.025715	20-21
GAGTACT	44040	0.0	40.60032	20-21
GTACTTT	44285	0.0	39.749027	22-23
ACGTGTG	1640	0.0	39.261032	2
TACTTTT	47105	0.0	38.45691	22-23
CATGGGG	19045	0.0	38.10078	24-25
TCGAGTG	885	0.0	37.705097	2
>>END_MODULE