FastQCFastQC Report
Thu 26 May 2016
SRR2132651_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132651_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3462826
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3091278.92701510269358No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1289193.72294189774479No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG398781.1516027660644803No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT169520.4895423564452849No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99140.2862979543297873No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA78930.22793521822927285No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56580.1633925585634392No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41000.11840040475611538No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39590.11432858595840509No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC36900.10656036428050383No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT35320.10199761697526818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT43600.087.040851
GTGGAAT1058300.067.379236
AACGAGT980250.067.262091
ACGAGTG1041950.066.98162
TGGAATC1066850.066.834837
GAATCAC1072550.066.497179
AGTGGAA1060350.066.486635
GGAATCA1074600.066.374688
CGAGTGG1063050.066.023473
GAGTGGA1085150.065.046044
AACGTGT12950.055.6974531
ACGTGTG14700.051.1538772
CGTGTGG14950.049.9840973
AGTACTT445900.041.6017720-21
ATCGAGT7500.041.4855041
GAGTACT441050.041.33463720-21
GTACTTT440850.040.6062522-23
TACTTTT463500.039.50915522-23
CATGGGG215750.039.2416124-25
TCGAGTG8750.038.1352162
Produced by FastQC (version 0.11.5)