##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132651_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3462826
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.531309109958165	33.0	30.0	34.0	16.0	34.0
2	31.117704152619854	33.0	31.0	34.0	25.0	34.0
3	31.88242435513653	34.0	31.0	34.0	28.0	34.0
4	35.94623986304828	37.0	35.0	37.0	35.0	37.0
5	35.68147547696592	37.0	35.0	37.0	35.0	37.0
6	36.135491070010445	37.0	36.0	37.0	35.0	37.0
7	36.16030086409193	37.0	35.0	37.0	35.0	37.0
8	36.32106291219946	37.0	37.0	37.0	35.0	37.0
9	38.23842636043509	39.0	39.0	39.0	37.0	39.0
10-11	38.28017853048348	39.0	39.0	39.0	37.0	39.0
12-13	38.25466237691412	39.0	39.0	39.0	37.0	39.0
14-15	39.78961980763688	41.0	40.0	41.0	38.0	41.0
16-17	39.66382876298145	41.0	39.5	41.0	37.5	41.0
18-19	39.46312217246839	41.0	39.0	41.0	36.0	41.0
20-21	39.35176673618599	41.0	39.0	41.0	35.0	41.0
22-23	38.96267427240063	40.5	39.0	41.0	35.0	41.0
24-25	38.908289645509186	41.0	39.0	41.0	35.0	41.0
26-27	38.84082191828293	41.0	39.0	41.0	35.0	41.0
28-29	38.70211093482607	41.0	39.0	41.0	35.0	41.0
30-31	38.537025683646824	41.0	38.5	41.0	35.0	41.0
32-33	38.38064430612454	41.0	38.0	41.0	35.0	41.0
34-35	38.28130044651391	41.0	38.0	41.0	35.0	41.0
36-37	38.09974439951647	40.5	38.0	41.0	35.0	41.0
38-39	37.6085132490053	40.0	36.0	41.0	34.0	41.0
40-41	37.40493183890845	40.0	35.0	41.0	33.0	41.0
42-43	37.27460288215463	40.0	35.0	41.0	33.0	41.0
44-45	37.10217362928429	40.0	35.0	41.0	33.0	41.0
46-47	36.982343900617586	40.0	35.0	41.0	33.0	41.0
48-49	36.81523790106693	40.0	35.0	41.0	33.0	41.0
50-51	36.54202723440335	39.0	35.0	41.0	32.5	41.0
52-53	35.98549753871549	39.0	35.0	41.0	30.5	41.0
54-55	35.029078128672936	39.0	35.0	41.0	25.0	41.0
56-57	34.35835672944583	39.0	35.0	41.0	19.0	41.0
58-59	34.009625664125195	38.0	35.0	41.0	14.5	41.0
60-61	33.76116588012219	38.0	35.0	40.5	11.0	41.0
62-63	33.492416165293896	37.0	34.5	40.0	7.5	41.0
64-65	33.19030078323311	36.5	34.0	40.0	7.0	41.0
66-67	32.83332457362859	36.0	34.0	39.5	3.5	41.0
68-69	32.52868914002609	35.5	34.0	39.0	2.0	41.0
70-71	32.206735336976216	35.0	34.0	39.0	2.0	41.0
72-73	31.865528906159305	35.0	33.0	38.0	2.0	40.0
74-75	31.673900161313334	35.0	34.0	37.0	2.0	40.0
76-77	31.37067080471268	35.0	33.0	37.0	2.0	39.0
78-79	31.04049524867839	35.0	33.0	36.0	2.0	39.0
80-81	30.739436229253215	35.0	33.0	36.0	2.0	38.5
82-83	30.426842844543735	35.0	33.0	36.0	2.0	37.0
84-85	30.121422647282884	35.0	33.0	35.0	2.0	37.0
86-87	29.933867598314208	35.0	33.0	35.0	2.0	36.5
88-89	29.72252590803003	35.0	33.0	35.0	2.0	36.0
90-91	29.52292419544037	35.0	32.0	35.0	2.0	36.0
92-93	29.371353484119616	35.0	32.0	35.0	2.0	36.0
94-95	29.182260240624274	35.0	32.0	35.0	2.0	35.5
96-97	28.96288537165887	35.0	32.0	35.0	2.0	35.0
98-99	28.82287660425329	35.0	32.0	35.0	2.0	35.0
100	28.733839644267427	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.2968914355342385
1101	2	1.9819026282324153
1101	3	1.2962355211936867
1101	4	0.5869029869231142
1101	5	0.865618036575583
1101	6	0.4076527935697811
1101	7	0.29096100560666116
1101	8	0.2318683460868698
1101	9	0.26425054925267943
1101	10-11	0.19052096495610016
1101	12-13	0.20320667165469075
1101	14-15	0.25516760912820047
1101	16-17	0.22241866187606263
1101	18-19	0.13981977082083574
1101	20-21	0.20345262502770112
1101	22-23	0.0782270103746896
1101	24-25	0.1422777607968655
1101	26-27	0.21427339315970073
1101	28-29	0.1670775278554899
1101	30-31	0.19852250471546995
1101	32-33	0.20903416700448219
1101	34-35	0.2693709426656028
1101	36-37	0.23349938776289036
1101	38-39	0.2644929625151775
1101	40-41	0.25505510746117466
1101	42-43	0.34384549927506924
1101	44-45	0.37135111467895854
1101	46-47	0.3120418998094081
1101	48-49	0.31644838691440214
1101	50-51	0.2661266378536382
1101	52-53	0.3211763158201464
1101	54-55	0.2900556512421062
1101	56-57	0.33963648946341607
1101	58-59	0.28455049244529107
1101	60-61	0.3433004320239448
1101	62-63	0.33892750762285573
1101	64-65	0.33454006630156385
1101	66-67	0.33344092465364383
1101	68-69	0.26832182281202677
1101	70-71	0.28382387444828794
1101	72-73	0.308318223423214
1101	74-75	0.3625490097378474
1101	76-77	0.3737830706514096
1101	78-79	0.259997600988477
1101	80-81	0.29861199168799146
1101	82-83	0.27237496540253403
1101	84-85	0.3358080874600837
1101	86-87	0.2528093152347566
1101	88-89	0.2683853398865601
1101	90-91	0.214787535483012
1101	92-93	0.24941040203468035
1101	94-95	0.24368872376788886
1101	96-97	0.21617083409805105
1101	98-99	0.23417278420485488
1101	100	0.21585547643835667
1102	1	3.369316003881732
1102	2	2.0449541238538025
1102	3	1.346659006667494
1102	4	0.6489299718525743
1102	5	0.8792518610424196
1102	6	0.43189251441777543
1102	7	0.2901180265872796
1102	8	0.23097142550577843
1102	9	0.2222154187684211
1102	10-11	0.23321375298193203
1102	12-13	0.18485378166288768
1102	14-15	0.25182676945389204
1102	16-17	0.32515883004741397
1102	18-19	0.18405602020720835
1102	20-21	0.20145994541557144
1102	22-23	0.1991188930413088
1102	24-25	0.17604353617712576
1102	26-27	0.15108407495324627
1102	28-29	0.117253526412334
1102	30-31	0.2297453207580702
1102	32-33	0.2982043057644077
1102	34-35	0.2283147820465814
1102	36-37	0.28861332573185905
1102	38-39	0.20987883669818785
1102	40-41	0.27543105867071205
1102	42-43	0.19566095495999036
1102	44-45	0.15362617015539826
1102	46-47	0.056402037930133986
1102	48-49	0.10390808549350794
1102	50-51	0.1849395975728143
1102	52-53	0.22706660803181222
1102	54-55	0.2153916869592578
1102	56-57	0.18696961612603502
1102	58-59	0.24399944970563325
1102	60-61	0.1950789371074606
1102	62-63	0.1591759697830355
1102	64-65	0.14149711290125566
1102	66-67	0.1705944448647969
1102	68-69	0.1485930313834558
1102	70-71	0.15950322862504862
1102	72-73	0.14689424704329923
1102	74-75	0.18950340600039084
1102	76-77	0.23383010971445017
1102	78-79	0.27498501757170146
1102	80-81	0.26014843685569744
1102	82-83	0.2502727578914943
1102	84-85	0.19250304375983873
1102	86-87	0.15684348715093677
1102	88-89	0.17666074604630566
1102	90-91	0.26383633225773906
1102	92-93	0.19113721922736104
1102	94-95	0.19007049561302125
1102	96-97	0.23145473978041053
1102	98-99	0.22480072209348378
1102	100	0.21315435589399812
1103	1	3.3030647944473195
1103	2	2.0042933252806456
1103	3	1.302305102605736
1103	4	0.5902126477670251
1103	5	0.9067419749598002
1103	6	0.43713962785113836
1103	7	0.32252899950342595
1103	8	0.279263811290555
1103	9	0.2504481416146547
1103	10-11	0.18756050137919544
1103	12-13	0.1931122060807695
1103	14-15	0.23468277186253772
1103	16-17	0.3209806422873669
1103	18-19	0.23825974254848603
1103	20-21	0.21621585525668507
1103	22-23	0.18706156099005966
1103	24-25	0.18936750309207895
1103	26-27	0.18279375037869272
1103	28-29	0.2310353212653311
1103	30-31	0.3035551525563349
1103	32-33	0.295615455640565
1103	34-35	0.3397853795184034
1103	36-37	0.3554056963130279
1103	38-39	0.3162961667156452
1103	40-41	0.29304748963163263
1103	42-43	0.2912920117900768
1103	44-45	0.3476379556918303
1103	46-47	0.24024610027388604
1103	48-49	0.3159589497811268
1103	50-51	0.3321543716895903
1103	52-53	0.3342522809508992
1103	54-55	0.28661136012658517
1103	56-57	0.26658554312479765
1103	58-59	0.21728023339421298
1103	60-61	0.11578508978256963
1103	62-63	0.12418661479977544
1103	64-65	0.12940420191912239
1103	66-67	0.1674922861022594
1103	68-69	0.2119538109645589
1103	70-71	0.19503100312117283
1103	72-73	0.1852661551656638
1103	74-75	0.2256861194503017
1103	76-77	0.1920798855235688
1103	78-79	0.271343006169495
1103	80-81	0.19074509614986113
1103	82-83	0.17226473686764976
1103	84-85	0.1983337513556087
1103	86-87	0.2075216552210719
1103	88-89	0.15928152872795565
1103	90-91	0.2075203056130448
1103	92-93	0.12859656204275893
1103	94-95	0.14438347206537827
1103	96-97	0.1991176663599532
1103	98-99	0.1812294298916406
1103	100	0.1761732038793724
1104	1	2.399643697599611
1104	2	1.6884112472181805
1104	3	1.1607105457705913
1104	4	0.5196789847227947
1104	5	0.8475763796556919
1104	6	0.35827856333474983
1104	7	0.20553212727200076
1104	8	0.16211841580813768
1104	9	0.13934058934543714
1104	10-11	0.13155336451339394
1104	12-13	0.0957157036611207
1104	14-15	0.12118134879296605
1104	16-17	0.1734260077436005
1104	18-19	0.1464555135890322
1104	20-21	0.08282773362113716
1104	22-23	0.016109897537894824
1104	24-25	0.023835310028182732
1104	26-27	0.079216137313459
1104	28-29	0.15520404038070268
1104	30-31	0.13590144315207198
1104	32-33	0.08607746806755046
1104	34-35	0.10531609683087595
1104	36-37	0.10751080625486509
1104	38-39	0.14353561538150217
1104	40-41	0.12679240509086753
1104	42-43	0.13772140236079622
1104	44-45	0.13072374615128268
1104	46-47	0.17576992277677306
1104	48-49	0.07305358353672631
1104	50-51	0.04120255818895657
1104	52-53	0.08834749115644058
1104	54-55	0.10211382380702361
1104	56-57	0.12927738462678917
1104	58-59	0.12386376661353893
1104	60-61	0.08625985880274101
1104	62-63	0.04441742726935871
1104	64-65	0.17199102845501102
1104	66-67	0.15394113680518728
1104	68-69	0.10578268843548244
1104	70-71	0.0034995119873073577
1104	72-73	0.045572102344976884
1104	74-75	0.10512328764325929
1104	76-77	0.1336243410100444
1104	78-79	0.20342797746324592
1104	80-81	0.13462981308672184
1104	82-83	0.18349497849350271
1104	84-85	0.11878703953589564
1104	86-87	0.08391566320859312
1104	88-89	0.11047925353147647
1104	90-91	0.14145664416987813
1104	92-93	0.1158404635206054
1104	94-95	0.2129890741167202
1104	96-97	0.2565895277375887
1104	98-99	0.266218449146816
1104	100	0.2649692495910614
1105	1	3.2465840067358833
1105	2	1.9313721420653458
1105	3	1.2149994251297827
1105	4	0.5766403398835749
1105	5	0.8321992715471254
1105	6	0.37807917773031363
1105	7	0.22940014379716445
1105	8	0.17001277866491193
1105	9	0.17617405398917185
1105	10-11	0.09547916800529777
1105	12-13	0.12083649313168365
1105	14-15	0.19318717938010366
1105	16-17	0.12549894999839495
1105	18-19	0.08080651814479722
1105	20-21	0.07114209447085074
1105	22-23	-0.04009793561603203
1105	24-25	-0.031867311795984676
1105	26-27	0.009510102249841168
1105	28-29	0.01451118736503787
1105	30-31	0.026926928849938747
1105	32-33	0.0030757480466760967
1105	34-35	0.030639114973524784
1105	36-37	-0.0037483493735237516
1105	38-39	-0.010833647449771888
1105	40-41	-0.00459877047592272
1105	42-43	0.012250921022975092
1105	44-45	0.09782852234911132
1105	46-47	0.12479186543110643
1105	48-49	0.03694091718337944
1105	50-51	0.054987014704124704
1105	52-53	-0.05068650317518575
1105	54-55	-0.042746063367836484
1105	56-57	-0.03164390642044168
1105	58-59	-0.042977244742523624
1105	60-61	-0.10969916346871145
1105	62-63	-0.09590773193931312
1105	64-65	-0.019976206845960576
1105	66-67	-0.047576885236253474
1105	68-69	-0.005799458065794738
1105	70-71	-0.054193101366095675
1105	72-73	0.012156513015774806
1105	74-75	-0.04536163108661384
1105	76-77	-0.056359369591380215
1105	78-79	0.027763795098024957
1105	80-81	-0.005009569999543828
1105	82-83	0.004299205899020109
1105	84-85	0.04756390960279688
1105	86-87	-0.008717698402186613
1105	88-89	-0.007528954355748851
1105	90-91	-0.006470916118118453
1105	92-93	0.0335594249197122
1105	94-95	-0.009856980743187194
1105	96-97	0.049343842437519214
1105	98-99	0.040526658093664736
1105	100	-0.028992998771350642
1106	1	1.7908960610457108
1106	2	1.4099522042271992
1106	3	1.0654936603148428
1106	4	0.45900759464416296
1106	5	0.7020291140825563
1106	6	0.29312987356740905
1106	7	0.18510202440521795
1106	8	0.13801790429122462
1106	9	0.20225090506125554
1106	10-11	0.17037033932602696
1106	12-13	0.11063121058607805
1106	14-15	0.18878855439297837
1106	16-17	0.19661364497515876
1106	18-19	0.18893617952629427
1106	20-21	0.2193038970433605
1106	22-23	0.12091810923017476
1106	24-25	0.1613906114165644
1106	26-27	0.12770516686545363
1106	28-29	0.1642873956133002
1106	30-31	0.20827462474156277
1106	32-33	0.14740219167386925
1106	34-35	0.23483379032023777
1106	36-37	0.24189350506111396
1106	38-39	0.2576703330898056
1106	40-41	0.2895537138775808
1106	42-43	0.2820178292282236
1106	44-45	0.3253539611475631
1106	46-47	0.28103614757078077
1106	48-49	0.32219766047189324
1106	50-51	0.393704767486561
1106	52-53	0.4409236917319035
1106	54-55	0.35891647078786093
1106	56-57	0.4026412928791814
1106	58-59	0.3927726355989378
1106	60-61	0.39402687453120677
1106	62-63	0.40612195700909126
1106	64-65	0.38276991864100296
1106	66-67	0.39262671603120936
1106	68-69	0.3805883234346936
1106	70-71	0.45579505395371456
1106	72-73	0.44118900560906127
1106	74-75	0.42213805083373757
1106	76-77	0.3961604649466146
1106	78-79	0.32133010410180063
1106	80-81	0.3056184555437653
1106	82-83	0.2808550697730503
1106	84-85	0.30214266612342655
1106	86-87	0.27100002863095085
1106	88-89	0.25916693272294467
1106	90-91	0.313227907895989
1106	92-93	0.2918642951735073
1106	94-95	0.3072242749746721
1106	96-97	0.39075720662962965
1106	98-99	0.33950694775819557
1106	100	0.40366310260214533
1107	1	2.7801615535440796
1107	2	1.7908315258188487
1107	3	1.182821775122406
1107	4	0.5069867205411
1107	5	0.8361661003839274
1107	6	0.3629772316887383
1107	7	0.1345423375167627
1107	8	0.13109813912488733
1107	9	0.06126199442850577
1107	10-11	0.06944666026535629
1107	12-13	0.04857119753976491
1107	14-15	0.10874419692396486
1107	16-17	0.14028226858899018
1107	18-19	0.10939932368806637
1107	20-21	0.1181740862149141
1107	22-23	-0.011020573092068275
1107	24-25	-0.014622434897852088
1107	26-27	0.06803523862018324
1107	28-29	-0.021102036872022722
1107	30-31	0.09650554450672644
1107	32-33	0.06057766460276781
1107	34-35	0.07842022215324107
1107	36-37	0.1359861338934678
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2216	86-87	-0.32391046177627913
2216	88-89	-0.4403682360549048
2216	90-91	-0.5033965608246902
2216	92-93	-0.5589139176957865
2216	94-95	-0.5634560519506167
2216	96-97	-0.719595223927044
2216	98-99	-0.7284302447203856
2216	100	-0.6949054899071783
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	438.0
3	113.0
4	198.0
5	534.0
6	1692.0
7	3555.0
8	12705.0
9	17115.0
10	3197.0
11	7666.0
12	5655.0
13	8587.0
14	7960.0
15	8163.0
16	10248.0
17	12977.0
18	17926.0
19	30043.0
20	64818.0
21	86236.0
22	41183.0
23	24989.0
24	24466.0
25	25812.0
26	26028.0
27	27441.0
28	31564.0
29	36115.0
30	40967.0
31	48904.0
32	62285.0
33	84115.0
34	127133.0
35	305426.0
36	404978.0
37	690866.0
38	874982.0
39	283882.0
40	1864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	14.120922693888001	46.008586027980186	23.473158959689925	16.397332318441887
2	15.91258915539969	45.35883658592793	22.25077351279639	16.477800745875996
3	16.69626203175045	24.384944396426377	19.92421199272153	38.99458157910164
4	36.91894540979833	25.31326069811023	20.751750589353772	17.016043302737668
5	15.979587758430691	44.461716795309265	21.542301890018088	18.016393556241958
6	39.059282793879916	23.25386837224856	22.079971676312933	15.606877157558596
7	17.197514400088252	23.187737414470146	43.035341654475275	16.579406530966327
8	38.27786900063705	23.677943968307964	21.569088368864044	16.47509866219094
9	37.920992853813615	24.611661111473694	21.708656455738755	15.758689578973936
10-11	15.595037117082983	46.39392796519375	21.848441128719724	16.162593789003548
12-13	15.469186150271483	24.254987111682773	32.71071084715201	27.565115890893736
14-15	15.81805701189868	34.99832939675484	21.876585593075703	27.307027998270772
16-17	27.115266548189254	23.88033646507217	32.77567223995661	16.228724746781964
18-19	16.06535126666811	24.127930230375487	32.89169969605741	26.915018806898992
20-21	27.168941596122004	35.18684782162967	21.92754571565702	15.716664866591316
22-23	26.77228842473728	24.37812496976738	32.74066571972691	16.108920885768423
24-25	15.923598240281203	35.09799510573156	21.707010401331168	27.271396252656068
26-27	16.26451921061006	29.149443258194317	38.44482801041693	16.141209520778695
28-29	26.75125749893295	24.07721323566359	33.11325778424905	16.05827148115441
30-31	23.63409250132695	26.152902282702044	33.54102978318864	16.671975432782357
32-33	18.985981392076877	27.3262358547614	34.87852118472022	18.8092615684415
34-35	19.11374523987644	27.160684213117804	35.146414635875544	18.57915591113021
36-37	19.133721994694508	26.839696825656272	35.802636343841705	18.22394483580752
38-39	20.083951759576436	26.86564599624237	34.990897597393115	18.05950464678808
40-41	18.133850252322887	28.773125988174396	34.846851920036386	18.24617183946633
42-43	18.3753099532592	27.15191272547913	35.71906196119608	18.753715360065588
44-45	18.292190583233534	27.841841571846444	34.85341695013343	19.01255089478659
46-47	19.08941593850601	27.090536426322508	35.398332696801916	18.42171493836956
48-49	18.191694707914735	27.24585561327451	35.42376361487786	19.13868606393289
50-51	19.068606124803754	28.256331566308486	34.26657446736929	18.408487841518475
52-53	19.42897374335007	27.637938234120302	34.28477904411956	18.648308978410068
54-55	19.180490557466896	28.917451709454344	32.080427039788866	19.821630693289897
56-57	19.41101624922173	29.962296538189193	31.148182459804346	19.47850475278473
58-59	21.14381342857411	29.87119536849127	29.53167268419149	19.453318518743128
60-61	20.349415623926973	30.763831552524827	29.199989141587636	19.686763681960567
62-63	19.23149951564064	30.703979399457925	29.235855789852295	20.828665295049138
64-65	19.855692258999056	30.515148399795773	29.666790650824414	19.962368690380757
66-67	19.95186296032542	29.62355558558886	30.581026111073566	19.843555343012145
68-69	20.5130641261572	29.53938586225543	30.23647616936762	19.71107384221975
70-71	18.803833471662216	31.083351960082734	30.35973884430983	19.75307572394522
72-73	18.776625667374375	29.541481172592082	31.001127981092893	20.680765178940657
74-75	18.837119141971613	30.54581919806747	29.94350706685081	20.6735545931101
76-77	19.81950957140743	29.78168599719157	30.23668070882201	20.162123722578993
78-79	19.383597801088367	30.178361235576716	29.680354417980343	20.757686545354574
80-81	20.216680415375123	31.19656792961778	28.820017156886458	19.76673449812064
82-83	20.18756667250006	30.64491908622505	29.28621511587948	19.881299125395415
84-85	19.574988697659986	31.29280431884396	28.224300225960164	20.90790675753589
86-87	19.72894684782122	31.438902213795682	28.616720648182508	20.215430290200587
88-89	21.469731895479725	30.705285446208425	27.951855661707413	19.873126996604437
90-91	20.315502108054737	31.533258574089196	28.09940649862491	20.051832819231166
92-93	19.41400954547791	31.64798696464084	27.966962226454132	20.971041263427118
94-95	20.02440047154799	32.066418724683324	27.572374010797546	20.33680679297114
96-97	20.132201437818427	30.983589324772883	28.277572192704863	20.606637044703827
98-99	20.93665630224266	31.04080013699868	27.121422013990383	20.901121546768273
100	19.644342867176267	32.63324724486581	27.00523894964561	20.717170938312314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	132078.0
1	73623.0
2	10673.5
3	5051.5
4	3799.5
5	3588.0
6	3617.0
7	4047.5
8	3882.5
9	3549.0
10	3782.0
11	5151.5
12	47712.0
13	52915.5
14	14231.5
15	12006.0
16	13155.5
17	14571.0
18	15795.0
19	17124.5
20	18693.5
21	20413.0
22	22291.0
23	24961.0
24	27887.0
25	30563.5
26	33636.5
27	36749.5
28	39813.5
29	43322.0
30	47582.0
31	52008.5
32	56876.5
33	62415.0
34	68945.0
35	74492.0
36	78662.5
37	82908.5
38	85962.5
39	87161.5
40	88539.5
41	90112.5
42	89530.5
43	90415.5
44	93953.5
45	96249.5
46	97706.0
47	102112.0
48	103775.0
49	110038.5
50	149224.0
51	149585.0
52	104746.5
53	89076.5
54	86880.5
55	85792.5
56	80118.5
57	72096.0
58	64142.0
59	53471.5
60	42780.5
61	34617.5
62	28095.0
63	21860.0
64	16711.0
65	12892.5
66	10107.0
67	7550.0
68	5688.5
69	4397.5
70	3227.0
71	2293.0
72	1633.0
73	1185.5
74	811.0
75	585.5
76	427.0
77	306.0
78	199.5
79	111.5
80	51.0
81	26.0
82	20.0
83	10.0
84	4.5
85	3.0
86	1.5
87	1.0
88	0.5
89	0.5
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.29571223041527356
2	0.0015016636700775608
3	2.59903327513424E-4
4	0.00389854991270136
5	5.7756295002983115E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.443907375074578E-4
16-17	0.0
18-19	2.8878147501491558E-5
20-21	2.3102518001193246E-4
22-23	0.002671228643887969
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.002151421988861121
36-37	0.0
38-39	1.1551259000596623E-4
40-41	2.8878147501491558E-5
42-43	1.5882981125820355E-4
44-45	0.00129951663756712
46-47	0.0014005901538223405
48-49	0.0020214703251044087
50-51	0.0010829305313059335
52-53	2.3102518001193246E-4
54-55	3.032205487656613E-4
56-57	4.042940650208818E-4
58-59	6.786364662850516E-4
60-61	0.0021081047676088835
62-63	1.29951663756712E-4
64-65	5.19806655026848E-4
66-67	1.1551259000596623E-4
68-69	1.0107351625522044E-4
70-71	0.0018482014400954597
72-73	5.7756295002983115E-5
74-75	1.0107351625522044E-4
76-77	0.001111808678807425
78-79	6.353192450328142E-4
80-81	0.002209178283864104
82-83	1.1551259000596623E-4
84-85	0.001371712006320849
86-87	0.006382070597829634
88-89	0.002079226620107392
90-91	0.004476112862731191
92-93	0.00704626799036394
94-95	0.005429091730280413
96-97	0.0023102518001193247
98-99	5.486848025283396E-4
100	0.00314771807766258
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3462826.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.67967317648751
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.6511873442363	45.74019584807969
2	10.74054199044844	11.745786515521226
3	2.580815730009618	4.233544819369918
4	0.9308717047491749	2.0359904233969863
5	0.4810613584105246	1.3152138927862302
6	0.3013798581524889	0.9887611287452555
7	0.21622418275141622	0.8276147351990383
8	0.17317562446512422	0.7575349238309701
9	0.13016812469011338	0.6405795464446506
>10	0.6846038664426768	7.312287696274707
>50	0.08302229962631402	2.883190978702021
>100	0.020303551855251418	2.0168160404352897
>500	0.0028475846413991137	1.1172698600203332
>1k	0.003427648178762841	3.4392350522385797
>5k	1.581991467121311E-4	0.6765958826652324
>10k+	2.109321956161748E-4	14.269382656289775
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	309127	8.92701510269358	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	128919	3.72294189774479	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	39878	1.1516027660644803	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16952	0.4895423564452849	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9914	0.2862979543297873	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	7893	0.22793521822927285	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5658	0.1633925585634392	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4100	0.11840040475611538	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3959	0.11432858595840509	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC	3690	0.10656036428050383	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	3532	0.10199761697526818	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.003378743257674512	2.8878147501491558E-5	0.0	0.0	2.8878147501491558E-5
2	0.003378743257674512	2.8878147501491558E-5	0.0	0.0	8.663444250447467E-5
3	0.003407621405176004	2.8878147501491558E-5	0.0	0.0	1.1551259000596623E-4
4	0.003465377700178987	2.8878147501491558E-5	0.0	0.0	1.1551259000596623E-4
5	0.003465377700178987	2.8878147501491558E-5	0.0	0.0	1.7326888500894934E-4
6	0.003465377700178987	2.8878147501491558E-5	0.0	0.0	1.7326888500894934E-4
7	0.003465377700178987	5.7756295002983115E-5	0.0	0.0	1.7326888500894934E-4
8	0.003465377700178987	8.663444250447467E-5	0.0	0.0	2.3102518001193246E-4
9	0.0034942558476804783	8.663444250447467E-5	0.0	0.0	2.59903327513424E-4
10-11	0.003508694921431224	8.663444250447467E-5	0.0	0.0	2.59903327513424E-4
12-13	0.0035664512164342074	8.663444250447467E-5	0.0	0.0	2.59903327513424E-4
14-15	0.003580890290184953	8.663444250447467E-5	0.0	0.0	2.887814750149156E-4
16-17	0.003580890290184953	8.663444250447467E-5	0.0	1.4439073750745779E-5	3.320986962671529E-4
18-19	0.0036097684376864446	1.0107351625522046E-4	0.0	2.8878147501491558E-5	3.465377700178987E-4
20-21	0.0036675247326894275	1.443907375074578E-4	0.0	2.8878147501491558E-5	4.0429406502088183E-4
22-23	0.0037397201014431566	1.7326888500894934E-4	0.0	2.8878147501491558E-5	4.3317221252237335E-4
24-25	0.003855232691449123	2.021470325104409E-4	0.0	2.8878147501491558E-5	4.4761128627311914E-4
26-27	0.003956306207704343	2.3102518001193246E-4	0.0	2.8878147501491558E-5	4.764894337746107E-4
28-29	0.0040285015764580726	2.454642537626782E-4	0.0	2.8878147501491558E-5	4.909285075253564E-4
30-31	0.00417289231396553	3.176596225164071E-4	0.0	2.8878147501491558E-5	4.909285075253564E-4
32-33	0.0044039174939774625	3.465377700178987E-4	0.0	2.8878147501491558E-5	5.342457287775939E-4
34-35	0.004606064526487904	3.465377700178987E-4	0.0	2.8878147501491558E-5	5.775629500298311E-4
36-37	0.0047215771164938695	3.465377700178987E-4	0.0	2.8878147501491558E-5	6.786364662850516E-4
38-39	0.005371335435277429	3.465377700178987E-4	0.0	2.8878147501491558E-5	6.930755400357974E-4
40-41	0.0063531924503281425	3.465377700178987E-4	0.0	2.8878147501491558E-5	6.930755400357974E-4
42-43	0.007205097801622143	3.7541591751939025E-4	0.0	2.8878147501491558E-5	7.219536875372889E-4
44-45	0.008374662775432551	4.764894337746107E-4	0.0	2.8878147501491558E-5	7.508318350387805E-4
46-47	0.009515349601741468	5.486848025283396E-4	0.0	2.8878147501491558E-5	7.941490562910177E-4
48-49	0.011045891419320521	5.486848025283396E-4	0.0	2.8878147501491558E-5	8.230272037925094E-4
50-51	0.013312825998187608	5.486848025283396E-4	0.0	2.8878147501491558E-5	8.374662775432551E-4
52-53	0.015781907609565136	5.631238762790853E-4	0.0	2.8878147501491558E-5	8.374662775432551E-4
54-55	0.01905957735098443	5.920020237805769E-4	0.0	2.8878147501491558E-5	8.374662775432551E-4
56-57	0.02349237299246338	6.353192450328142E-4	0.0	2.8878147501491558E-5	8.663444250447467E-4
58-59	0.02890702564899305	6.353192450328142E-4	0.0	2.8878147501491558E-5	9.241007200477298E-4
60-61	0.036444222146882345	6.497583187835599E-4	0.0	2.8878147501491558E-5	9.674179412999672E-4
62-63	0.04551196046235069	6.930755400357974E-4	0.0	2.8878147501491558E-5	9.818570150507129E-4
64-65	0.05878146923928606	7.79709982540272E-4	0.0	2.8878147501491558E-5	0.0010107351625522046
66-67	0.07473664573386014	7.941490562910177E-4	0.0	2.8878147501491558E-5	0.0010251742363029503
68-69	0.09567330267244153	8.085881300417635E-4	0.0	2.8878147501491558E-5	0.0010684914575551875
70-71	0.12245778448007494	8.085881300417635E-4	0.0	2.8878147501491558E-5	0.0010829305313059335
72-73	0.15517672559926488	8.374662775432551E-4	0.0	2.8878147501491558E-5	0.0011551259000596621
74-75	0.19702116132892614	8.663444250447467E-4	0.0	2.8878147501491558E-5	0.0012273212688133912
76-77	0.24641723263022744	8.663444250447467E-4	0.0	2.8878147501491558E-5	0.001241760342564137
78-79	0.31085881877980587	8.663444250447467E-4	0.0	2.8878147501491558E-5	0.0012850775638163742
80-81	0.3903892369989136	8.663444250447467E-4	0.0	2.8878147501491558E-5	0.0013283947850686116
82-83	0.48913806238026397	9.529788675492213E-4	0.0	2.8878147501491558E-5	0.0013572729325701033
84-85	0.6085636413726823	9.674179412999672E-4	0.0	2.8878147501491558E-5	0.0013572729325701033
86-87	0.7548170193939863	0.001039613310053696	0.0	2.8878147501491558E-5	0.0013861510800715947
88	0.8831515068906147	0.0010973696050566792	0.0	2.8878147501491558E-5	0.0014439073750745779
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	4360	0.0	87.04085	1
GTGGAAT	105830	0.0	67.37923	6
AACGAGT	98025	0.0	67.26209	1
ACGAGTG	104195	0.0	66.9816	2
TGGAATC	106685	0.0	66.83483	7
GAATCAC	107255	0.0	66.49717	9
AGTGGAA	106035	0.0	66.48663	5
GGAATCA	107460	0.0	66.37468	8
CGAGTGG	106305	0.0	66.02347	3
GAGTGGA	108515	0.0	65.04604	4
AACGTGT	1295	0.0	55.697453	1
ACGTGTG	1470	0.0	51.153877	2
CGTGTGG	1495	0.0	49.984097	3
AGTACTT	44590	0.0	41.60177	20-21
ATCGAGT	750	0.0	41.485504	1
GAGTACT	44105	0.0	41.334637	20-21
GTACTTT	44085	0.0	40.60625	22-23
TACTTTT	46350	0.0	39.509155	22-23
CATGGGG	21575	0.0	39.24161	24-25
TCGAGTG	875	0.0	38.135216	2
>>END_MODULE