FastQCFastQC Report
Thu 26 May 2016
SRR2132650_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132650_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4303607
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2928896.80566324945563No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA926882.153728256320803No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG213050.49504985004439295No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158460.36820276572651733No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89640.2082903945457845No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65060.15117551393517112No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA54500.1266379574157213No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA51670.12006207815908841No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT40900.087.795431
AACGAGT836250.075.3248141
ACGAGTG894200.074.559582
CGAGTGG912900.073.500773
GTGGAAT929500.073.293736
TGGAATC934900.072.895527
GAATCAC939700.072.5181669
GGAATCA941000.072.4179848
AGTGGAA938150.071.841475
GAGTGGA939800.071.762014
AACGTGT13300.047.8324241
ATCGAGT7250.044.8488541
AGTACTT433650.041.99722320-21
GAGTACT428100.041.8061320-21
GTACTTT432250.040.74054322-23
TCGAGTG8800.040.589072
CGTGTGG17150.040.2838863
ACGTGTG16850.040.1643522
CATGGGG206350.038.78629324-25
TACTTTT470450.038.2416522-23