##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132650_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4303607
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.46196760066614	31.0	30.0	34.0	16.0	34.0
2	31.058919645776207	33.0	31.0	34.0	25.0	34.0
3	31.8448633901748	34.0	31.0	34.0	28.0	34.0
4	35.92282148439669	37.0	35.0	37.0	35.0	37.0
5	35.67550289791796	37.0	35.0	37.0	35.0	37.0
6	36.12080726702043	37.0	36.0	37.0	35.0	37.0
7	36.1762877047091	37.0	36.0	37.0	35.0	37.0
8	36.31457612184384	37.0	37.0	37.0	35.0	37.0
9	38.227189889783155	39.0	39.0	39.0	37.0	39.0
10-11	38.26855739847993	39.0	39.0	39.0	37.0	39.0
12-13	38.23419110992244	39.0	39.0	39.0	37.0	39.0
14-15	39.7578433625561	41.0	40.0	41.0	37.5	41.0
16-17	39.663006171334885	41.0	40.0	41.0	37.0	41.0
18-19	39.54982030190024	41.0	39.5	41.0	36.5	41.0
20-21	39.49169069108773	41.0	39.5	41.0	36.0	41.0
22-23	39.19290004872657	40.5	39.0	41.0	36.0	41.0
24-25	39.152587120524714	41.0	39.0	41.0	35.0	41.0
26-27	39.08029427408218	41.0	39.0	41.0	35.0	41.0
28-29	38.95549442595478	41.0	39.0	41.0	35.0	41.0
30-31	38.8212494774732	41.0	39.0	41.0	35.0	41.0
32-33	38.69672370176924	41.0	39.0	41.0	35.0	41.0
34-35	38.623864818511535	41.0	39.0	41.0	35.0	41.0
36-37	38.486802233568255	40.5	38.5	41.0	35.0	41.0
38-39	38.090601093454865	40.0	37.0	41.0	34.5	41.0
40-41	37.90310511624319	40.0	37.0	41.0	34.0	41.0
42-43	37.78553292621747	40.0	36.0	41.0	34.0	41.0
44-45	37.627282184455964	40.0	36.0	41.0	33.5	41.0
46-47	37.52251239483531	40.0	35.0	41.0	33.0	41.0
48-49	37.38066963363523	40.0	35.0	41.0	33.0	41.0
50-51	37.15503576418571	40.0	35.0	41.0	33.0	41.0
52-53	36.69611607193687	40.0	35.0	41.0	32.0	41.0
54-55	35.876888967789114	39.5	35.0	41.0	29.0	41.0
56-57	35.272704733494486	39.0	35.0	41.0	25.5	41.0
58-59	34.93622465991899	39.0	35.0	41.0	24.0	41.0
60-61	34.6893558124615	39.0	35.0	41.0	23.0	41.0
62-63	34.41416409537395	38.0	35.0	41.0	22.0	41.0
64-65	34.12875478639197	37.5	35.0	40.0	22.0	41.0
66-67	33.80993699006439	37.0	35.0	40.0	20.0	41.0
68-69	33.50435390592124	36.5	34.0	39.5	20.0	41.0
70-71	33.17667923674257	36.0	34.0	39.0	18.5	41.0
72-73	32.82907337960924	35.5	34.0	39.0	17.0	41.0
74-75	32.640221562981935	35.0	34.0	38.5	14.5	40.0
76-77	32.33602545492653	35.0	34.0	37.0	9.5	39.5
78-79	32.00098707897817	35.0	34.0	37.0	6.0	39.0
80-81	31.69505405581876	35.0	34.0	36.5	2.0	39.0
82-83	31.396182086328977	35.0	34.0	36.0	2.0	38.0
84-85	31.087294680950187	35.0	33.0	36.0	2.0	37.0
86-87	30.87062015653381	35.0	33.0	35.0	2.0	37.0
88-89	30.666492897701858	35.0	33.0	35.0	2.0	36.5
90-91	30.48660879118377	35.0	33.0	35.0	2.0	36.0
92-93	30.330908119630813	35.0	33.0	35.0	2.0	36.0
94-95	30.161749551016158	35.0	33.0	35.0	2.0	36.0
96-97	29.98597223212993	35.0	33.0	35.0	2.0	35.5
98-99	29.8537138730372	35.0	33.0	35.0	2.0	35.0
100	29.761670849591983	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.411286389512604
1101	2	2.0949812306263134
1101	3	1.35331417304646
1101	4	0.5968741440123893
1101	5	0.8593298937925411
1101	6	0.44170229947695105
1101	7	0.2671972467126622
1101	8	0.18177716747435824
1101	9	0.2211836271973695
1101	10-11	0.14744677496154424
1101	12-13	0.21191128341823884
1101	14-15	0.2687965676846318
1101	16-17	0.23255047215311464
1101	18-19	0.14576344010224318
1101	20-21	0.1818757296248137
1101	22-23	0.16528655990720864
1101	24-25	0.1879328911841185
1101	26-27	0.21778955875500117
1101	28-29	0.21548011884187446
1101	30-31	0.22793779311440687
1101	32-33	0.1842843847221758
1101	34-35	0.1733707957816577
1101	36-37	0.2518883930808684
1101	38-39	0.19166787771217741
1101	40-41	0.2572433033405872
1101	42-43	0.2694894454590582
1101	44-45	0.2687152464797222
1101	46-47	0.26191045987068406
1101	48-49	0.23317140469397657
1101	50-51	0.2513515202673702
1101	52-53	0.2383624923116443
1101	54-55	0.16055280197242894
1101	56-57	0.1935190798639539
1101	58-59	0.10444965996683209
1101	60-61	0.15285132855949968
1101	62-63	0.1551260913965038
1101	64-65	0.17575747170313605
1101	66-67	0.12899739327843918
1101	68-69	0.0657422033105135
1101	70-71	0.10416447030178233
1101	72-73	0.11788701767333976
1101	74-75	0.13351356158144512
1101	76-77	0.18525074438053224
1101	78-79	0.07487226263435787
1101	80-81	0.1963193237275611
1101	82-83	0.12731822047627972
1101	84-85	0.22139850676097694
1101	86-87	0.1829362134057071
1101	88-89	0.19830362176005778
1101	90-91	0.22367446734082463
1101	92-93	0.21616249626620743
1101	94-95	0.23551749382992426
1101	96-97	0.2409435257734529
1101	98-99	0.22838122172928266
1101	100	0.17435083432884113
1102	1	3.3375555002609616
1102	2	2.028365781059602
1102	3	1.3127933996346108
1102	4	0.5951585761585605
1102	5	0.8505211202780885
1102	6	0.4136960228591988
1102	7	0.24688714882829288
1102	8	0.20431161391758224
1102	9	0.22935244977595204
1102	10-11	0.20346740971297805
1102	12-13	0.18014138570105587
1102	14-15	0.23002456861561882
1102	16-17	0.2667885436779187
1102	18-19	0.14876791119107224
1102	20-21	0.12943510130284608
1102	22-23	0.13198557629079488
1102	24-25	0.12687253111696606
1102	26-27	0.1518470695030203
1102	28-29	0.15982034020234437
1102	30-31	0.1948088209507759
1102	32-33	0.20664173427088883
1102	34-35	0.22251296980458335
1102	36-37	0.22325231012935376
1102	38-39	0.21790837595195
1102	40-41	0.24036181815402102
1102	42-43	0.22575697249310878
1102	44-45	0.25141457371914555
1102	46-47	0.19978293234044742
1102	48-49	0.1671488361606137
1102	50-51	0.1979420036003674
1102	52-53	0.26360416774577544
1102	54-55	0.24515658903123239
1102	56-57	0.1532519083640409
1102	58-59	0.11493904713614711
1102	60-61	0.13706229349723031
1102	62-63	0.15029435670529523
1102	64-65	0.17743119964362108
1102	66-67	0.14351015329562244
1102	68-69	0.15295759465877978
1102	70-71	0.19803638949650804
1102	72-73	0.15026400149496055
1102	74-75	0.1641661207334053
1102	76-77	0.24171400967249923
1102	78-79	0.25092424998819496
1102	80-81	0.23095993358950295
1102	82-83	0.22985230265996037
1102	84-85	0.1613735925643347
1102	86-87	0.16893699101836646
1102	88-89	0.18563418325169323
1102	90-91	0.220390520348424
1102	92-93	0.2075051427647736
1102	94-95	0.21375972705947532
1102	96-97	0.2355490941823426
1102	98-99	0.2818201078078211
1102	100	0.23678079452415446
1103	1	3.3943831763322407
1103	2	2.0460959822364977
1103	3	1.3367630832889112
1103	4	0.6021162461366387
1103	5	0.8964454554412598
1103	6	0.45370578998415567
1103	7	0.3010007344152541
1103	8	0.23660670261782002
1103	9	0.20839448385849124
1103	10-11	0.1580826294367128
1103	12-13	0.1695138704272665
1103	14-15	0.2591983688655404
1103	16-17	0.3402433997000358
1103	18-19	0.23527504439924485
1103	20-21	0.20057884513861524
1103	22-23	0.22269507690152324
1103	24-25	0.21121624052025112
1103	26-27	0.20858289746659864
1103	28-29	0.2580542122091245
1103	30-31	0.30019699946636536
1103	32-33	0.2592669022840042
1103	34-35	0.3305817627713523
1103	36-37	0.3373193612171548
1103	38-39	0.29088458096255465
1103	40-41	0.3354158889803074
1103	42-43	0.3696895680784351
1103	44-45	0.43681004944733814
1103	46-47	0.3816509062415179
1103	48-49	0.3847564137842241
1103	50-51	0.38006428282874083
1103	52-53	0.3707705143740654
1103	54-55	0.4172619389514267
1103	56-57	0.3151335652420073
1103	58-59	0.34101282606267347
1103	60-61	0.308064059568963
1103	62-63	0.3674097803796528
1103	64-65	0.41914152112043723
1103	66-67	0.39083108605606043
1103	68-69	0.3703866803242377
1103	70-71	0.33624767783992837
1103	72-73	0.2992213777841428
1103	74-75	0.3038568246921898
1103	76-77	0.3942116874485109
1103	78-79	0.3094878974917563
1103	80-81	0.23891006949094518
1103	82-83	0.28225059318804213
1103	84-85	0.2064129226835938
1103	86-87	0.21736401129696503
1103	88-89	0.20929794097298426
1103	90-91	0.2549335668699797
1103	92-93	0.19421079728873636
1103	94-95	0.3093159383827029
1103	96-97	0.3360324712220617
1103	98-99	0.35083502911663444
1103	100	0.35309481884407035
1104	1	2.52317014490054
1104	2	1.7828903142789336
1104	3	1.21548603307998
1104	4	0.5570028307540866
1104	5	0.874084343824471
1104	6	0.3516309343348709
1104	7	0.2079156624613887
1104	8	0.19105825284312772
1104	9	0.12445904027207888
1104	10-11	0.14914515808334983
1104	12-13	0.15651661746996837
1104	14-15	0.15633566760082118
1104	16-17	0.23148629341348936
1104	18-19	0.22977868345712693
1104	20-21	0.22881620795335067
1104	22-23	0.22380703653955436
1104	24-25	0.24021702472498419
1104	26-27	0.2642542278961244
1104	28-29	0.2612482232879074
1104	30-31	0.28532393565511427
1104	32-33	0.23742441355757649
1104	34-35	0.2602309166110004
1104	36-37	0.28126197126572805
1104	38-39	0.30393359001661224
1104	40-41	0.2830723213402706
1104	42-43	0.2647890368742907
1104	44-45	0.297274398978125
1104	46-47	0.2850726390892362
1104	48-49	0.2747750874446169
1104	50-51	0.19330092859271986
1104	52-53	0.22800725055318338
1104	54-55	0.20627943681039085
1104	56-57	0.27369230434112524
1104	58-59	0.19181084591031095
1104	60-61	0.20044503116566403
1104	62-63	0.08685370903300083
1104	64-65	0.21609274568940862
1104	66-67	0.1807648716269341
1104	68-69	0.13941062354918898
1104	70-71	0.09495198127720528
1104	72-73	0.09671413243627569
1104	74-75	0.10645012118792607
1104	76-77	0.1775414506983779
1104	78-79	0.2086747326016365
1104	80-81	0.2059402728016373
1104	82-83	0.21109212942024058
1104	84-85	0.15058369444057362
1104	86-87	0.11710274287968403
1104	88-89	0.15060827184386838
1104	90-91	0.16507105149232615
1104	92-93	0.11743084267026305
1104	94-95	0.14517454952516573
1104	96-97	0.1292755370681533
1104	98-99	0.16393339735587986
1104	100	0.12851538074486868
1105	1	3.3225000166441987
1105	2	2.0201159635735486
1105	3	1.2953712384824279
1105	4	0.5800253113627747
1105	5	0.844563665510691
1105	6	0.39716494124818524
1105	7	0.21543986153731254
1105	8	0.15766552514005383
1105	9	0.12073805327523246
1105	10-11	0.10591575873326775
1105	12-13	0.12221126531978399
1105	14-15	0.17854028601233551
1105	16-17	0.1304688104132481
1105	18-19	0.13482439419249204
1105	20-21	0.13636110506721622
1105	22-23	0.12740421746432418
1105	24-25	0.12309452044730307
1105	26-27	0.12504387222025315
1105	28-29	0.13273475908479782
1105	30-31	0.1521816924266659
1105	32-33	0.15330006753667647
1105	34-35	0.1378423334465566
1105	36-37	0.11721620135789834
1105	38-39	0.19838271894850124
1105	40-41	0.18923425914014302
1105	42-43	0.18684047254641456
1105	44-45	0.1292263016196813
1105	46-47	0.15174841585121612
1105	48-49	0.10441388880754943
1105	50-51	0.11576957950965294
1105	52-53	0.08244564214675165
1105	54-55	0.12972895775990878
1105	56-57	0.0741920282029156
1105	58-59	0.14492879144205517
1105	60-61	0.10567246303101996
1105	62-63	0.06682575003952707
1105	64-65	0.11964036966806901
1105	66-67	0.1540654258757641
1105	68-69	0.1311597371948494
1105	70-71	0.11839802584393766
1105	72-73	0.11492753056346316
1105	74-75	0.10469836943617139
1105	76-77	0.08491377376035558
1105	78-79	0.16642683199166441
1105	80-81	0.1343468937082548
1105	82-83	0.16856594560095672
1105	84-85	0.14757122384189714
1105	86-87	0.09029649081481139
1105	88-89	0.15290494043363978
1105	90-91	0.15001374048674165
1105	92-93	0.13177494046263405
1105	94-95	0.09611515762094669
1105	96-97	0.03634040982268161
1105	98-99	0.07702975726258288
1105	100	0.019063194568545327
1106	1	1.7646827373983527
1106	2	1.3745836966127136
1106	3	1.0186265160234207
1106	4	0.42101830807006024
1106	5	0.6809191118661317
1106	6	0.22796405204729808
1106	7	0.16298921051522797
1106	8	0.1317414749302941
1106	9	0.12328882296885979
1106	10-11	0.09898116179866889
1106	12-13	0.052877748129127156
1106	14-15	0.11702187520820928
1106	16-17	0.16286923495653127
1106	18-19	0.1413699657380647
1106	20-21	0.1570682036314608
1106	22-23	0.12373509379519732
1106	24-25	0.11196212645776882
1106	26-27	0.11645957363595016
1106	28-29	0.1083321282535934
1106	30-31	0.16189888642956873
1106	32-33	0.12748115028919926
1106	34-35	0.11578697567691165
1106	36-37	0.13688985460297687
1106	38-39	0.1149018818962304
1106	40-41	0.12672243377117098
1106	42-43	0.11754957682694567
1106	44-45	0.1508449982383766
1106	46-47	0.114451787126022
1106	48-49	0.11012483166135212
1106	50-51	0.12225472885193511
1106	52-53	0.16290854475251138
1106	54-55	0.2550279766303589
1106	56-57	0.2954908966446368
1106	58-59	0.29659905168374223
1106	60-61	0.24845163366733658
1106	62-63	0.2520915910196564
1106	64-65	0.2103481368044129
1106	66-67	0.23851084532118705
1106	68-69	0.24694029511826443
1106	70-71	0.30415535088697254
1106	72-73	0.2645949995166461
1106	74-75	0.25398842944051836
1106	76-77	0.2238815820138811
1106	78-79	0.22387882015794247
1106	80-81	0.2481695382451896
1106	82-83	0.2639371702578934
1106	84-85	0.2871952356087668
1106	86-87	0.22993245330791723
1106	88-89	0.3035970796972087
1106	90-91	0.31534919653648075
1106	92-93	0.29823134120475103
1106	94-95	0.3659361474419356
1106	96-97	0.4023816272924705
1106	98-99	0.3484348450962429
1106	100	0.38136909973159305
1107	1	2.887373512665242
1107	2	1.8643172651519002
1107	3	1.2224830810041105
1107	4	0.509990499032881
1107	5	0.8356100493439556
1107	6	0.40027261779848544
1107	7	0.19050778873638308
1107	8	0.14595672884109945
1107	9	0.10749302625971069
1107	10-11	0.11778394371619783
1107	12-13	0.1069155331388032
1107	14-15	0.1110983880786307
1107	16-17	0.18108628332580423
1107	18-19	0.16725705678909009
1107	20-21	0.15821812708816907
1107	22-23	0.06516224587809205
1107	24-25	0.09142571517194398
1107	26-27	0.13509203407168968
1107	28-29	0.05355679039863048
1107	30-31	0.11090128917905417
1107	32-33	0.05284739216597245
1107	34-35	0.06929225260140015
1107	36-37	0.12933969594040917
1107	38-39	0.126298467356051
1107	40-41	0.08266036781214581
1107	42-43	0.08495151524761724
1107	44-45	0.2003996785307649
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2216	88-89	-0.30374592926070676
2216	90-91	-0.3036456905639966
2216	92-93	-0.2903750834265182
2216	94-95	-0.3388234407959132
2216	96-97	-0.40925146011701585
2216	98-99	-0.4019211860361871
2216	100	-0.40577377809451676
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	558.0
3	161.0
4	214.0
5	513.0
6	1634.0
7	3155.0
8	11394.0
9	15973.0
10	3212.0
11	7231.0
12	5622.0
13	8215.0
14	7931.0
15	8139.0
16	10150.0
17	13132.0
18	18147.0
19	31096.0
20	69351.0
21	94426.0
22	45194.0
23	26327.0
24	24879.0
25	25627.0
26	26336.0
27	28459.0
28	33027.0
29	39005.0
30	45498.0
31	55333.0
32	71487.0
33	97947.0
34	146437.0
35	310856.0
36	458540.0
37	872510.0
38	1227746.0
39	454884.0
40	3261.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.37387134083718	41.772245116164065	25.792400588854502	18.061482954144246
2	16.892708470090426	40.0594159944511	24.97179657283339	18.076078962625086
3	18.23435987819486	25.353593913925447	20.964612144033072	35.44743406384662
4	32.38771971039973	26.181186299640125	22.754702100570963	18.676391889389183
5	17.19178819069678	38.96087630678173	23.3682583005372	20.47907720198429
6	35.25712268801496	23.419610573177337	24.310212340485553	17.013054398322154
7	18.897473677313005	23.40820153884869	39.34982910846646	18.344495675371846
8	34.4645782014947	23.590188416367944	23.936641984270405	18.00859139786695
9	34.1438704788797	25.23971171159448	23.658456731760126	16.9579610777657
10-11	16.98238245267284	41.739243383515266	23.84467261996739	17.433701543844503
12-13	16.90226361282524	24.844171412491896	32.078312448139435	26.17525252654343
14-15	17.160981503857236	33.03012594107259	23.798389720234223	26.010502834835954
16-17	25.830283468129224	24.558222169759752	32.05861922055821	17.55287514155282
18-19	17.437221846697433	24.687965234743785	32.22602575002782	25.648787168530955
20-21	25.873310163567826	33.16975311582381	23.784034611047456	17.17290210956091
22-23	25.573207854072265	24.958266527245264	32.027605437434644	17.440920181247822
24-25	17.245103932584925	33.17944691511098	23.575503060572213	25.99994609173189
26-27	17.60821794369235	28.479075807804943	36.48064286539175	17.432063383110957
28-29	25.449407903649195	24.689382650413943	32.47524692658972	17.385962519347142
30-31	23.15638951233233	26.075591939505628	32.796349666686574	17.97166888147547
32-33	19.616033713115534	27.203808340306168	33.90453635752521	19.27562158905309
34-35	19.467336941474613	27.03153717484234	34.093751644733594	19.40737423894945
36-37	19.484527746144106	27.127918511146582	34.25123390681352	19.136319835895797
38-39	19.955033006080257	26.942658020573933	33.96116415067289	19.14114482267292
40-41	19.12014018011872	27.846078381710594	33.90088451405635	19.132896924114338
42-43	19.24574016174611	27.19367394224807	34.155444173262985	19.405141722742826
44-45	19.214930307980065	27.55622317107898	33.70752901999435	19.5213175009466
46-47	19.655170490760295	27.15611099402423	33.926282203958735	19.262436311256735
48-49	19.232964655414897	27.503035008117173	33.84436336631052	19.41963697015741
50-51	19.624277271964164	27.855915056618656	33.258763504042285	19.261044167374898
52-53	19.7809808355617	27.67960793717626	33.01181151762387	19.52759970963817
54-55	19.94121657386826	28.458179372868354	31.52505789347229	20.075546159791095
56-57	20.327693893667135	29.015232516948934	30.419734503023584	20.23733908636035
58-59	21.046245204998964	29.385007170209015	29.41767771667735	20.151069908114668
60-61	20.661581049325097	29.76053959687316	29.23266803903512	20.345211314766622
62-63	20.10240731071831	29.905829980906677	29.183643736323944	20.80811897205107
64-65	20.40597168494294	29.851999825261593	29.432302345770122	20.30972614402534
66-67	20.330504732143407	29.330529014142698	29.85948158047699	20.479484673236904
68-69	20.530839112310932	29.383168957441807	29.74680625053226	20.339185679715
70-71	19.70213460094653	30.007533366283095	29.863256156993305	20.427075875777067
72-73	19.769344403903474	29.382523130330927	29.998123664999266	20.850008800766336
74-75	19.88447819187783	29.724523846646633	29.548008959004818	20.84298900247072
76-77	20.41627212850771	29.46160790323349	29.565126970609185	20.55699299764962
78-79	20.263713032372237	29.704621466851023	29.13216776871439	20.899497732062343
80-81	20.565747165832562	30.19725166361199	28.747412723908667	20.48958844664678
82-83	20.49734629209353	30.14310573865932	28.788856123046724	20.570691846200425
84-85	20.404881921929125	30.483314825361663	28.253075193020834	20.858728059688385
86-87	20.411409428880226	30.584767273333142	28.397437997765927	20.606385300020705
88-89	21.15631931481023	30.159810303616226	28.11696549454939	20.566904887024158
90-91	20.760370658039434	30.677500666619807	27.900958015442338	20.661170659898417
92-93	20.287215364350015	30.81107892769863	27.885047858189317	21.016657849762037
94-95	20.522903489326854	31.01077533096257	27.773203223641246	20.693117956069333
96-97	20.549081108186616	30.463316013318465	28.010277218607467	20.977325659887455
98-99	20.939594149663485	30.60863376236746	27.40641718996143	21.045354898007623
100	20.47366482590192	31.1954901457314	27.33074394702873	21.000101081337945
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	96383.0
1	54116.5
2	8416.5
3	4210.0
4	3286.5
5	2994.0
6	2996.5
7	3396.0
8	3291.5
9	2981.5
10	3063.5
11	3974.0
12	31564.0
13	35501.5
14	11395.5
15	10600.0
16	12134.5
17	13986.0
18	15817.5
19	18019.5
20	20705.0
21	23712.0
22	27180.0
23	31786.5
24	36383.0
25	41016.0
26	47260.0
27	53857.0
28	59186.0
29	63798.0
30	69341.0
31	75169.0
32	81377.5
33	88736.5
34	96450.5
35	102808.5
36	109022.0
37	114811.5
38	118831.0
39	122396.5
40	125853.0
41	129095.5
42	130161.5
43	130530.0
44	132495.5
45	133672.5
46	134000.0
47	136108.0
48	135107.5
49	137541.0
50	158531.5
51	154606.5
52	124302.0
53	111853.0
54	107232.0
55	102642.0
56	94534.0
57	83502.5
58	75541.0
59	64229.5
60	51250.5
61	42381.0
62	33926.5
63	25795.0
64	19257.0
65	14521.5
66	10773.0
67	8016.0
68	6034.0
69	4248.5
70	3092.0
71	2448.5
72	1960.5
73	1493.0
74	1039.5
75	717.0
76	488.5
77	344.5
78	222.5
79	123.0
80	67.0
81	36.5
82	22.0
83	12.5
84	7.0
85	5.0
86	5.5
87	6.0
88	3.0
89	2.5
90	2.0
91	0.5
92	0.0
93	1.0
94	1.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.29194115540754534
2	0.001208288768003212
3	2.788358695392028E-4
4	0.003671338948932837
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.6265425723120162E-4
16-17	3.485448369240035E-5
18-19	0.0
20-21	2.3236322461600236E-4
22-23	0.0024862865033912253
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.002288777762467623
36-37	0.0
38-39	1.394179347696014E-4
40-41	2.3236322461600234E-5
42-43	2.4398138584680247E-4
44-45	0.0015219791212348153
46-47	0.001394179347696014
48-49	0.001777578668312418
50-51	8.01653124925208E-4
52-53	2.555995470776026E-4
54-55	3.02072192000803E-4
56-57	4.066356430780041E-4
58-59	8.481257698484086E-4
60-61	0.002277159601236823
62-63	1.8589057969280187E-4
64-65	7.203259963096073E-4
66-67	1.7427241846200176E-4
68-69	1.277997735388013E-4
70-71	0.0019983237316976202
72-73	1.0456345107720106E-4
74-75	9.294528984640093E-5
76-77	0.001208288768003212
78-79	6.389988676940064E-4
80-81	0.002195832472621222
82-83	1.394179347696014E-4
84-85	0.001301234057849613
86-87	0.005902025905246459
88-89	0.002288777762467623
90-91	0.004170919881857242
92-93	0.007447241348942875
94-95	0.004937718523090049
96-97	0.002509522825852825
98-99	5.925262227708059E-4
100	0.0032995577895472334
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4303607.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.179120832295645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.67653183434624	54.12967957254185
2	9.8982908887706	12.507306321896975
3	2.095541422200473	3.9718339416685304
4	0.6795635274678075	1.7173690486043867
5	0.34817777260256905	1.0998782783188634
6	0.23279219948452315	0.8824563900029135
7	0.1605083578342023	0.709854385394029
8	0.1270864674812125	0.6423369028116107
9	0.09716391484308694	0.5524857644769245
>10	0.6373749719011368	8.28449701159352
>50	0.03167381831540702	1.3323419363404674
>100	0.011479826504007317	1.4218152262235557
>500	0.002054229884334675	0.8963005880505625
>1k	0.0014673069703592773	1.4502019519534601
>5k	1.4673069703592299E-4	0.6045868622848004
>10k+	1.4673069703592299E-4	9.797055817837586
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	292889	6.80566324945563	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	92688	2.153728256320803	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	21305	0.49504985004439295	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	15846	0.36820276572651733	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8964	0.2082903945457845	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6506	0.15117551393517112	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	5450	0.1266379574157213	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	5167	0.12006207815908841	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.00197508740923602	2.3236322461600234E-5	0.0	2.3236322461600234E-5	0.0
2	0.0019983237316976202	2.3236322461600234E-5	0.0	4.647264492320047E-5	0.0
3	0.0020447963766208207	2.3236322461600234E-5	0.0	6.97089673848007E-5	0.0
4	0.0020447963766208207	2.3236322461600234E-5	0.0	6.97089673848007E-5	0.0
5	0.0020447963766208207	2.3236322461600234E-5	0.0	9.294528984640093E-5	0.0
6	0.0020447963766208207	2.3236322461600234E-5	0.0	9.294528984640093E-5	0.0
7	0.0020447963766208207	2.3236322461600234E-5	0.0	9.294528984640093E-5	0.0
8	0.0020447963766208207	2.3236322461600234E-5	0.0	1.1618161230800117E-4	0.0
9	0.0020447963766208207	2.3236322461600234E-5	0.0	1.1618161230800117E-4	0.0
10-11	0.002068032699082421	4.647264492320047E-5	0.0	1.1618161230800117E-4	0.0
12-13	0.0021493598276980217	9.294528984640093E-5	0.0	1.277997735388013E-4	0.0
14-15	0.002184214311390422	9.294528984640093E-5	0.0	1.5103609600040154E-4	0.0
16-17	0.0022423051175444225	1.0456345107720104E-4	0.0	1.6265425723120165E-4	0.0
18-19	0.0023236322461600233	1.394179347696014E-4	0.0	1.6265425723120165E-4	0.0
20-21	0.002381723052314024	1.7427241846200176E-4	0.0	1.6265425723120165E-4	0.0
22-23	0.0024281956972372243	1.8589057969280187E-4	0.0	1.9750874092360198E-4	0.0
24-25	0.002451432019698825	2.672177083084027E-4	0.0	2.0912690215440212E-4	0.0
26-27	0.0025327591483144257	2.788358695392028E-4	0.0	2.0912690215440212E-4	0.0
28-29	0.002544377309545226	3.02072192000803E-4	0.0	2.0912690215440212E-4	0.0
30-31	0.0026257044381608266	3.136903532316032E-4	0.0	2.3236322461600236E-4	0.0
32-33	0.0027070315667764274	3.369266756932034E-4	0.0	2.555995470776026E-4	0.0
34-35	0.002892922146469229	3.7178115938560374E-4	0.0	3.02072192000803E-4	0.0
36-37	0.0031252853710852313	4.0663564307800407E-4	0.0	3.485448369240035E-4	0.0
38-39	0.0035086846917016354	4.1825380430880423E-4	0.0	3.485448369240035E-4	0.0
40-41	0.004275483332934443	4.4149012677040445E-4	0.0	3.7178115938560374E-4	0.0
42-43	0.005111990941552052	4.8796277169360495E-4	0.0	3.9501748184720396E-4	0.0
44-45	0.006134389129862462	4.8796277169360495E-4	0.0	4.0663564307800407E-4	0.0
46-47	0.007400768704019674	5.228172553860053E-4	0.0	4.8796277169360495E-4	0.0
48-49	0.00886465701910049	5.809080615400058E-4	0.0	4.8796277169360495E-4	0.0
50-51	0.011211525587722114	6.273807064632064E-4	0.0	5.111990941552052E-4	0.0
52-53	0.013337649092958535	6.738533513864068E-4	0.0	5.692899003092057E-4	0.0
54-55	0.016160862272042963	6.854715126172069E-4	0.0	6.157625452324063E-4	0.0
56-57	0.020192364219130603	7.203259963096073E-4	0.0	6.506170289248066E-4	0.0
58-59	0.024723447099142648	7.203259963096073E-4	0.0	6.738533513864068E-4	0.0
60-61	0.030381491618542304	7.319441575404074E-4	0.0	6.738533513864068E-4	0.0
62-63	0.03744533364686878	7.551804800020076E-4	0.0	6.97089673848007E-4	0.0
64-65	0.047471806789049276	7.667986412328077E-4	0.0	7.667986412328077E-4	0.0
66-67	0.06035634759400661	7.900349636944079E-4	0.0	8.01653124925208E-4	0.0
68-69	0.07599439261066357	7.900349636944079E-4	0.0	8.597439310792087E-4	0.0
70-71	0.09811537159410699	7.900349636944079E-4	0.0	9.062165760024091E-4	0.0
72-73	0.12561555922741086	7.900349636944079E-4	0.0	9.526892209256096E-4	0.0
74-75	0.16007502543796404	7.900349636944079E-4	0.0	9.8754370461801E-4	0.0
76-77	0.20249293209161523	8.248894473868084E-4	0.0	0.001103725316926011	0.0
78-79	0.25642443652498936	8.365076086176085E-4	0.0	0.0011850524455416119	0.0
80-81	0.3255873503319425	8.597439310792087E-4	0.0	0.0012315250904648125	0.0
82-83	0.412630614273097	8.597439310792087E-4	0.0	0.001277997735388013	0.0
84-85	0.5196571155312277	8.713620923100088E-4	0.0	0.0013012340578496132	0.0
86-87	0.6496178670589577	9.062165760024091E-4	0.0	0.0013360885415420135	0.0
88	0.7647306085337253	9.062165760024091E-4	0.0	0.0013709430252344139	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	4090	0.0	87.79543	1
AACGAGT	83625	0.0	75.324814	1
ACGAGTG	89420	0.0	74.55958	2
CGAGTGG	91290	0.0	73.50077	3
GTGGAAT	92950	0.0	73.29373	6
TGGAATC	93490	0.0	72.89552	7
GAATCAC	93970	0.0	72.518166	9
GGAATCA	94100	0.0	72.417984	8
AGTGGAA	93815	0.0	71.84147	5
GAGTGGA	93980	0.0	71.76201	4
AACGTGT	1330	0.0	47.832424	1
ATCGAGT	725	0.0	44.848854	1
AGTACTT	43365	0.0	41.997223	20-21
GAGTACT	42810	0.0	41.80613	20-21
GTACTTT	43225	0.0	40.740543	22-23
TCGAGTG	880	0.0	40.58907	2
CGTGTGG	1715	0.0	40.283886	3
ACGTGTG	1685	0.0	40.164352	2
CATGGGG	20635	0.0	38.786293	24-25
TACTTTT	47045	0.0	38.24165	22-23
>>END_MODULE