FastQCFastQC Report
Thu 26 May 2016
SRR2132649_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132649_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5189289
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3168936.106674729428251No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA865161.6672033490522498No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG556751.0728830095991955No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT178150.34330329260906456No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA81600.15724697545270652No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTACCGTAGATCTCGTA81280.15663032064700966TruSeq Adapter, Index 10 (97% over 37bp)
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70940.13670466223792893No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69910.1347198045820921No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT54040.10413758031206201No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC53010.10215272265622516No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52670.10149752692517222No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT48750.087.2150041
GTGGAAT1270950.061.6517526
TGGAATC1283800.061.0236747
GAATCAC1290850.060.7595679
GGAATCA1291850.060.7052578
AACGAGT1200550.060.31521
AGTGGAA1286300.060.287625
ACGAGTG1272850.060.274842
CGAGTGG1295200.059.6592673
GAGTGGA1325850.058.5317734
AACGTGT17100.046.3097531
CGTGTGG18550.044.842463
ATCGAGT9300.044.6024781
GAGTACT479700.040.9235920-21
AGTACTT489600.040.77763420-21
GTACTTT482200.039.9061722-23
TACGGCG5500.038.4512066
TCGAGTG11650.037.516052
CATGGGG224800.037.2643324-25
TACTTTT527600.037.16703422-23
Produced by FastQC (version 0.11.5)