FastQCFastQC Report
Thu 26 May 2016
SRR2132648_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132648_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2706570
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2128717.864973010119819No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1126614.162500877494393No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG351921.3002434816021753No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT117480.43405491082809605No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT116250.42951041354925235No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63500.2346142904118497No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT53050.1960045371078524No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC49360.18237104527132128No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47980.17727234100725273No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT40650.1501900929959321No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29960.11069360851557507No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC28190.10415396608992192No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG27200.10049619998743796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT33500.085.265511
GTGGAAT853550.060.5983056
TGGAATC859950.060.1309247
AACGAGT793500.059.9897421
ACGAGTG843700.059.8697282
GAATCAC864100.059.8475769
GGAATCA865250.059.778898
AGTGGAA862100.059.376965
CGAGTGG861300.059.0807153
GAGTGGA880150.058.210554
AACGTGT9550.055.7725641
CGTGTGG10900.051.7424283
ATCGAGT5400.048.0080951
ACGTGTG12650.043.4713822
ATCTGCG14900.041.32388794
TCGAGTG6500.041.2163542
AGTACTT322450.041.12384420-21
GAGTACT318650.040.67032220-21
GTACTTT318800.039.93545522-23
TACTTTT339300.038.52682522-23
Produced by FastQC (version 0.11.5)