##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132648_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2706570 Sequences flagged as poor quality 0 Sequence length 100 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.553146602526446 33.0 30.0 34.0 16.0 34.0 2 31.146730732994158 33.0 31.0 34.0 25.0 34.0 3 31.92804545975164 34.0 31.0 34.0 28.0 34.0 4 35.98221882308604 37.0 35.0 37.0 35.0 37.0 5 35.72130076074146 37.0 35.0 37.0 35.0 37.0 6 36.170281574095625 37.0 37.0 37.0 35.0 37.0 7 36.21532160631352 37.0 36.0 37.0 35.0 37.0 8 36.36332258171782 37.0 37.0 37.0 35.0 37.0 9 38.2889361812183 39.0 39.0 39.0 37.0 39.0 10-11 38.32647446768419 39.0 39.0 39.0 37.0 39.0 12-13 38.29511374174693 39.0 39.0 39.0 37.0 39.0 14-15 39.83840968458233 41.0 40.0 41.0 38.0 41.0 16-17 39.69735809530144 41.0 39.5 41.0 37.5 41.0 18-19 39.449017206279535 41.0 39.0 41.0 36.0 41.0 20-21 39.324907724536956 41.0 39.0 41.0 35.0 41.0 22-23 38.81828439685654 40.5 39.0 41.0 35.0 41.0 24-25 38.74236265827228 41.0 39.0 41.0 35.0 41.0 26-27 38.699439881473594 41.0 39.0 41.0 35.0 41.0 28-29 38.590160978655646 41.0 39.0 41.0 35.0 41.0 30-31 38.434496059588334 41.0 38.5 41.0 35.0 41.0 32-33 38.30338712835803 41.0 38.0 41.0 35.0 41.0 34-35 38.21719519539491 41.0 38.0 41.0 35.0 41.0 36-37 38.03494940090225 40.5 38.0 41.0 35.0 41.0 38-39 37.580722279490274 40.0 36.0 41.0 34.5 41.0 40-41 37.394448693364666 40.0 35.0 41.0 33.5 41.0 42-43 37.265329365211315 40.0 35.0 41.0 33.0 41.0 44-45 37.0904175764898 40.0 35.0 41.0 33.0 41.0 46-47 36.96341033115715 40.0 35.0 41.0 33.0 41.0 48-49 36.794406573633786 40.0 35.0 41.0 33.0 41.0 50-51 36.5240036282084 39.5 35.0 41.0 32.5 41.0 52-53 35.96216724488929 39.0 35.0 41.0 30.5 41.0 54-55 34.9979082381021 39.0 35.0 41.0 25.0 41.0 56-57 34.35116549728993 39.0 35.0 41.0 17.5 41.0 58-59 33.9960974591457 38.5 35.0 41.0 9.0 41.0 60-61 33.73591187369992 38.0 35.0 41.0 7.0 41.0 62-63 33.46770229478639 37.5 35.0 40.0 3.5 41.0 64-65 33.170432872602596 37.0 34.5 40.0 2.0 41.0 66-67 32.82033810320812 36.0 34.0 40.0 2.0 41.0 68-69 32.525311556693524 36.0 34.0 39.0 2.0 41.0 70-71 32.20854771906879 35.0 34.0 39.0 2.0 41.0 72-73 31.877841881052404 35.0 33.5 38.5 2.0 40.5 74-75 31.698393353949832 35.0 34.0 37.5 2.0 40.0 76-77 31.401449066530702 35.0 34.0 37.0 2.0 39.5 78-79 31.06724415034527 35.0 33.0 36.5 2.0 39.0 80-81 30.761903811835644 35.0 33.0 36.0 2.0 39.0 82-83 30.443924783027967 35.0 33.0 36.0 2.0 37.5 84-85 30.12693150371134 35.0 33.0 35.5 2.0 37.0 86-87 29.933429950084424 35.0 33.0 35.0 2.0 37.0 88-89 29.722621066515924 35.0 33.0 35.0 2.0 36.0 90-91 29.52880620120669 35.0 32.0 35.0 2.0 36.0 92-93 29.3815415821501 35.0 32.0 35.0 2.0 36.0 94-95 29.183838585368196 35.0 32.0 35.0 2.0 36.0 96-97 28.9650025678257 35.0 32.0 35.0 2.0 35.0 98-99 28.83187539949087 35.0 32.0 35.0 2.0 35.0 100 28.747976959768266 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 3.3382200712605865 1101 2 1.999524317171737 1101 3 1.285381222069617 1101 4 0.5540605809690362 1101 5 0.8319195868816678 1101 6 0.34806462470202604 1101 7 0.22656332643887822 1101 8 0.15534230640773217 1101 9 0.17620249985818504 1101 10-11 0.13631301316471678 1101 12-13 0.1740024402553999 1101 14-15 0.2336016583611169 1101 16-17 0.13307932272077494 1101 18-19 0.06438943097644767 1101 20-21 0.14116430595073126 1101 22-23 0.0661815478558836 1101 24-25 0.10364411667676166 1101 26-27 0.14634627053758464 1101 28-29 0.18378221514823423 1101 30-31 0.21153892329211743 1101 32-33 0.15189075261947238 1101 34-35 0.150799354020819 1101 36-37 0.231662699629112 1101 38-39 0.2135732276271085 1101 40-41 0.21290989395171067 1101 42-43 0.2600268078179937 1101 44-45 0.30883337552340606 1101 46-47 0.3124442617868368 1101 48-49 0.296794292945485 1101 50-51 0.25120197673770406 1101 52-53 0.1886072972658681 1101 54-55 0.21152301609514268 1101 56-57 0.05608975988720033 1101 58-59 0.0649031645332272 1101 60-61 0.03567236601318058 1101 62-63 0.1211074111211019 1101 64-65 0.16486345476525344 1101 66-67 0.08846245089659277 1101 68-69 0.08511958278906206 1101 70-71 0.0961348940457043 1101 72-73 0.20283358652192618 1101 74-75 0.2117372501832584 1101 76-77 0.1720057048811654 1101 78-79 0.0914967460441467 1101 80-81 0.17834549661048982 1101 82-83 0.18529053117183025 1101 84-85 0.1343145813129567 1101 86-87 0.05772371791754338 1101 88-89 0.10078750876683884 1101 90-91 0.1443202692213248 1101 92-93 0.21423327058775854 1101 94-95 0.1889130265350758 1101 96-97 0.21589646675329988 1101 98-99 0.29383712756715497 1101 100 0.3133629145831591 1102 1 3.3206437278421745 1102 2 2.028258267937833 1102 3 1.3130909411495537 1102 4 0.612645839054359 1102 5 0.8662601085610717 1102 6 0.43320224346726377 1102 7 0.29189130718465606 1102 8 0.21479214341793096 1102 9 0.23134639338121588 1102 10-11 0.23572600733912452 1102 12-13 0.19260748365137204 1102 14-15 0.24863958670801622 1102 16-17 0.283741243352722 1102 18-19 0.1595096028368701 1102 20-21 0.17168940324422977 1102 22-23 0.09219945123567186 1102 24-25 0.14275592247714997 1102 26-27 0.08451327385100171 1102 28-29 -0.018849622676334832 1102 30-31 0.06306291918725293 1102 32-33 0.04054061155976996 1102 34-35 0.09476541940440342 1102 36-37 0.06444778069916168 1102 38-39 0.039352985259881734 1102 40-41 0.09168802051627978 1102 42-43 0.07170995716056439 1102 44-45 0.1482169527367958 1102 46-47 0.12331829575818887 1102 48-49 0.17647281569146855 1102 50-51 0.12289357044178217 1102 52-53 0.10226887837858811 1102 54-55 0.14441023663276553 1102 56-57 0.06136755675569816 1102 58-59 0.01914119353726562 1102 60-61 0.0276898698000565 1102 62-63 0.11379675443758686 1102 64-65 0.09557866012686134 1102 66-67 0.06321490223317738 1102 68-69 0.034180457869183556 1102 70-71 0.06814750257110802 1102 72-73 -0.01939681574700103 1102 74-75 0.044625527507268004 1102 76-77 0.0685177353302322 1102 78-79 0.04756184522000595 1102 80-81 -0.02888268818016826 1102 82-83 -0.034436032090063406 1102 84-85 -0.0777974459870272 1102 86-87 -0.05271577826061247 1102 88-89 -0.02890152864900486 1102 90-91 -0.008664028138785085 1102 92-93 -0.043475436388710875 1102 94-95 -0.011191283245466366 1102 96-97 0.06089484221021024 1102 98-99 0.11189989769930975 1102 100 0.102501476938059 1103 1 3.3361851462688676 1103 2 2.0254656671630578 1103 3 1.2826285522164795 1103 4 0.5759478555892628 1103 5 0.7912210870472975 1103 6 0.3665761322817289 1103 7 0.2187627806372916 1103 8 0.20683852838807582 1103 9 0.19383851645307715 1103 10-11 0.09883253424748517 1103 12-13 0.11763186306233564 1103 14-15 0.22306027366564507 1103 16-17 0.22087292769293754 1103 18-19 0.1504517722444163 1103 20-21 0.14990344118648125 1103 22-23 0.05800635501124418 1103 24-25 0.07239855328647593 1103 26-27 0.09628553828008535 1103 28-29 0.13968428635482866 1103 30-31 0.13925176224910274 1103 32-33 0.15221018851932655 1103 34-35 0.18992827992746442 1103 36-37 0.15265949738280682 1103 38-39 0.14737112329381574 1103 40-41 0.18496516819335085 1103 42-43 0.1258321806509599 1103 44-45 0.09398709912065328 1103 46-47 0.028619549279149226 1103 48-49 0.05200385867040325 1103 50-51 0.030207545175855444 1103 52-53 -0.0615070686735848 1103 54-55 -0.08388420552238784 1103 56-57 0.009416625629476982 1103 58-59 0.033519573284984006 1103 60-61 0.02713041343166367 1103 62-63 0.06254416281292663 1103 64-65 0.06414649863445732 1103 66-67 0.08139936822185234 1103 68-69 0.08271001075429751 1103 70-71 0.04293895758584654 1103 72-73 0.020573715558256822 1103 74-75 0.06288406450029882 1103 76-77 0.09891403860886072 1103 78-79 0.045237695360317076 1103 80-81 0.03296666929517045 1103 82-83 0.0502480185915779 1103 84-85 0.14706835538896001 1103 86-87 0.15956067152440667 1103 88-89 0.1691862278683125 1103 90-91 0.10540824423434003 1103 92-93 0.05423137763427732 1103 94-95 0.1351215984594205 1103 96-97 0.10023700908447353 1103 98-99 0.184755683243651 1103 100 0.2562903671410126 1104 1 2.5379449083623555 1104 2 1.773433456819756 1104 3 1.2104256833073208 1104 4 0.5164336398681257 1104 5 0.7954210806499304 1104 6 0.3592923778310677 1104 7 0.23444436284408 1104 8 0.18282390189921927 1104 9 0.17949262101927843 1104 10-11 0.16675071865823554 1104 12-13 0.1495825326062814 1104 14-15 0.10508346021087078 1104 16-17 0.15519469938058705 1104 18-19 0.17170240506423085 1104 20-21 0.12703001608285547 1104 22-23 0.1602953103601834 1104 24-25 0.14757454501686595 1104 26-27 0.2086346585812393 1104 28-29 0.16918674792439248 1104 30-31 0.1893333691815826 1104 32-33 0.18693240980429238 1104 34-35 0.2314587382152098 1104 36-37 0.2507754522433032 1104 38-39 0.17152668758014045 1104 40-41 0.1614873672629784 1104 42-43 0.12949825045487984 1104 44-45 0.15434299388367378 1104 46-47 0.17085895471802104 1104 48-49 0.12908849976388836 1104 50-51 0.07328788059769664 1104 52-53 0.09500609310141073 1104 54-55 -0.06400219107486294 1104 56-57 0.030672859520493034 1104 58-59 0.012414534574830327 1104 60-61 0.02039907306293287 1104 62-63 -0.10346990032964243 1104 64-65 -0.03269754068533359 1104 66-67 -0.08792516418655083 1104 68-69 -0.07783541616002765 1104 70-71 -0.16882293426435524 1104 72-73 -0.13718956481773859 1104 74-75 -0.20077310162491102 1104 76-77 -0.17426214801180961 1104 78-79 -0.08317610692078858 1104 80-81 -0.07786105160736412 1104 82-83 -0.009922096063021968 1104 84-85 -0.00612654285847114 1104 86-87 -0.026255371482097445 1104 88-89 0.045247411540668026 1104 90-91 0.07218616527057264 1104 92-93 0.12743420645866266 1104 94-95 0.09558579634486009 1104 96-97 0.13039844005423973 1104 98-99 0.15312494958565281 1104 100 0.15481906696233239 1105 1 3.2616568838024165 1105 2 1.9538668851146035 1105 3 1.2559989770715205 1105 4 0.566332554835391 1105 5 0.8425722902827815 1105 6 0.3458931244657464 1105 7 0.224713708764952 1105 8 0.16576104142132664 1105 9 0.14564075644566543 1105 10-11 0.08446708708142125 1105 12-13 0.10285643573335079 1105 14-15 0.15670632833391807 1105 16-17 0.1419384361314684 1105 18-19 0.09504291091923989 1105 20-21 0.08411233177456268 1105 22-23 -0.013887041294623259 1105 24-25 -0.03870844652438166 1105 26-27 -0.009334242511030766 1105 28-29 -0.05819541522074445 1105 30-31 -0.006278503711449446 1105 32-33 0.009657990084505741 1105 34-35 0.005477625358565774 1105 36-37 -0.0424139651525266 1105 38-39 0.06632382476955456 1105 40-41 0.03498669231461804 1105 42-43 0.026040314412661303 1105 44-45 -0.024059883994212328 1105 46-47 0.012989470494481736 1105 48-49 -8.839077792686112E-4 1105 50-51 0.03470457000469196 1105 52-53 -0.017056145948110668 1105 54-55 0.08100758353627668 1105 56-57 -0.02002088080595854 1105 58-59 0.0744775613504558 1105 60-61 -0.020375736921643295 1105 62-63 0.06671770750050143 1105 64-65 0.026781971401653948 1105 66-67 0.0887295128350516 1105 68-69 0.07344731637169843 1105 70-71 0.04158525502694488 1105 72-73 0.11804147135717358 1105 74-75 0.08611765139780303 1105 76-77 0.1378059843959356 1105 78-79 0.15745158954182514 1105 80-81 0.20408252217822564 1105 82-83 0.04579769336060835 1105 84-85 0.08198512726803031 1105 86-87 0.0837380654304063 1105 88-89 0.1050114126913293 1105 90-91 0.12319441253591634 1105 92-93 0.06806649961102451 1105 94-95 0.048668894705656385 1105 96-97 0.08617890059587197 1105 98-99 0.07854647867806364 1105 100 -0.034763135432964276 1106 1 1.8975729881356287 1106 2 1.412949407587977 1106 3 1.0339813104927131 1106 4 0.46147507510919894 1106 5 0.683464454355736 1106 6 0.23340161092396983 1106 7 0.1671546015434231 1106 8 0.11860975728579604 1106 9 0.12149252036945057 1106 10-11 0.10057667344607069 1106 12-13 0.03722712914380821 1106 14-15 0.09314482985118389 1106 16-17 0.1100429315427931 1106 18-19 0.033411993361845305 1106 20-21 0.11150379266189958 1106 22-23 -0.06822627106521395 1106 24-25 -0.05541526450652157 1106 26-27 -0.08650943311457837 1106 28-29 -0.08940094387563846 1106 30-31 -0.05639828181322315 1106 32-33 -0.06635520854684529 1106 34-35 -0.1090935235711683 1106 36-37 -0.06862177631908395 1106 38-39 -0.029225001011838003 1106 40-41 -0.025997718407225534 1106 42-43 -0.038337727272200084 1106 44-45 0.015915558269306018 1106 46-47 -0.04148259346744965 1106 48-49 0.00927535808141755 1106 50-51 0.10002203573475299 1106 52-53 0.23365342948014245 1106 54-55 0.37950914381578116 1106 56-57 0.36154843658481894 1106 58-59 0.43336909627349485 1106 60-61 0.47817850851932064 1106 62-63 0.4511665531271376 1106 64-65 0.4400390325956991 1106 66-67 0.4724343149569492 1106 68-69 0.49556003128260784 1106 70-71 0.5493916906653311 1106 72-73 0.5365213370238351 1106 74-75 0.4761099897772425 1106 76-77 0.42271345204908783 1106 78-79 0.49173918478216194 1106 80-81 0.5359567683810553 1106 82-83 0.578956320855319 1106 84-85 0.4727525365650713 1106 86-87 0.5219130883016057 1106 88-89 0.4852337410006804 1106 90-91 0.5491451262585727 1106 92-93 0.5060294915875225 1106 94-95 0.48626613185278345 1106 96-97 0.5670075537050963 1106 98-99 0.4961571467013357 1106 100 0.5003451502379619 1107 1 2.8779633247909686 1107 2 1.8560691519559178 1107 3 1.1682964225322578 1107 4 0.5186745169461773 1107 5 0.832629274043839 1107 6 0.39974504159769 1107 7 0.17177560626044652 1107 8 0.16284252494631346 1107 9 0.08789186170877628 1107 10-11 0.09685112404122265 1107 12-13 0.05069682237826356 1107 14-15 0.09334594311038558 1107 16-17 0.1927807494462428 1107 18-19 0.09675895442843796 1107 20-21 0.1239762944046845 1107 22-23 -0.08289244074818214 1107 24-25 -0.11927161420059917 1107 26-27 -0.08070917337768435 1107 28-29 -0.0779380188985499 1107 30-31 -0.011214207152434597 1107 32-33 -0.14420125605203538 1107 34-35 -0.0892238284494411 1107 36-37 -0.030427891341709312 1107 38-39 -0.03212262433672208 1107 40-41 -0.04776737354222149 1107 42-43 0.008723365156150464 1107 44-45 -0.003775258108980495 1107 46-47 0.004672250767086439 1107 48-49 0.06309698182622725 1107 50-51 -0.0757515425832338 1107 52-53 0.06266463638381481 1107 54-55 -0.031053076852053607 1107 56-57 -0.13031396655939176 1107 58-59 -0.13083138710605624 1107 60-61 -0.1256770862628258 1107 62-63 -0.12764566370715613 1107 64-65 -0.1477337530647489 1107 66-67 -0.1695439789656561 1107 68-69 -0.05764816753288926 1107 70-71 -0.05195718363121671 1107 72-73 0.024186724665220538 1107 74-75 -0.053005253428260346 1107 76-77 -0.09516563464900685 1107 78-79 -0.037385435845099124 1107 80-81 0.015350149454704365 1107 82-83 -0.022638312664419402 1107 84-85 -0.035108986634902806 1107 86-87 -0.04196537795324318 1107 88-89 -0.03815691059448412 1107 90-91 -0.016332337850673895 1107 92-93 0.0036350052756084494 1107 94-95 -0.010767771950206395 1107 96-97 0.0698205443241342 1107 98-99 0.03442097898820862 1107 100 0.07503837879580644 1108 1 -2.9224130213471007 1108 2 -0.9691353825930946 1108 3 0.06575794489516795 1108 4 -0.02492831654583938 1108 5 0.49729176883951 1108 6 0.2575993515330879 1108 7 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-0.20338275107302195 2214 74-75 -0.26739375493132655 2214 76-77 -0.3368525761916388 2214 78-79 -0.371054989457793 2214 80-81 -0.35161020713773183 2214 82-83 -0.3664375395987811 2214 84-85 -0.3223216175452279 2214 86-87 -0.2790105078027132 2214 88-89 -0.28508389317237715 2214 90-91 -0.3053322426766272 2214 92-93 -0.27892358854628085 2214 94-95 -0.350091584023005 2214 96-97 -0.27397258305817473 2214 98-99 -0.28076183142246336 2214 100 -0.3233516023744549 2215 1 -2.8595501615615326 2215 2 -4.636262144884991 2215 3 -4.403390029738638 2215 4 -1.8952123973835953 2215 5 -2.1575711851244606 2215 6 -1.020452726924347 2215 7 -0.6080196878305628 2215 8 -0.44940692518146363 2215 9 -0.41840140813461346 2215 10-11 -0.3511874863712734 2215 12-13 -0.31303952897533094 2215 14-15 -0.403871755223598 2215 16-17 -0.375258757045394 2215 18-19 -0.36880276073449636 2215 20-21 -0.33851574747304625 2215 22-23 -0.09272781270172459 2215 24-25 -0.13067307717025045 2215 26-27 -0.14426304495749775 2215 28-29 -0.23741216496764395 2215 30-31 -0.09640260899768549 2215 32-33 -0.07607214164561782 2215 34-35 -0.07679109159487751 2215 36-37 -0.1458705869597665 2215 38-39 -0.1862432997009762 2215 40-41 -0.20688561720782417 2215 42-43 -0.17322845661852426 2215 44-45 -0.12121365688298624 2215 46-47 -0.27423370885779974 2215 48-49 -0.19098951245440077 2215 50-51 -0.18488422709423702 2215 52-53 -0.3143309162212091 2215 54-55 -0.3219987751783435 2215 56-57 -0.31369957519103764 2215 58-59 -0.4433646438069587 2215 60-61 -0.3406675509689876 2215 62-63 -0.3369881800852923 2215 64-65 -0.2737520494593042 2215 66-67 -0.35284883207732065 2215 68-69 -0.34718151745492776 2215 70-71 -0.326265739332289 2215 72-73 -0.3433330920528732 2215 74-75 -0.41884865540719574 2215 76-77 -0.38900600585701994 2215 78-79 -0.32285150178544697 2215 80-81 -0.3864613757129547 2215 82-83 -0.3098199365862655 2215 84-85 -0.3747525518833186 2215 86-87 -0.38979903434880114 2215 88-89 -0.3575090405529373 2215 90-91 -0.4339847718738312 2215 92-93 -0.5400747479437165 2215 94-95 -0.567311931910929 2215 96-97 -0.5204871914163434 2215 98-99 -0.567953173078827 2215 100 -0.6307136785127909 2216 1 -4.44574499787899 2216 2 -1.9625640016332966 2216 3 -4.516593983902808 2216 4 -1.8340072304854687 2216 5 -0.3975679570137558 2216 6 -0.35837985389120064 2216 7 -0.28035715945148354 2216 8 -0.26450195503939966 2216 9 -0.2641745563088662 2216 10-11 -0.27586074456437615 2216 12-13 -0.2648508937207339 2216 14-15 -0.404825050141163 2216 16-17 -0.3574094033252706 2216 18-19 -0.3565672606623522 2216 20-21 -0.3656365754897095 2216 22-23 -0.008632918775226983 2216 24-25 -0.04865896786257906 2216 26-27 -0.04314216390461212 2216 28-29 -0.19705425602332838 2216 30-31 -0.23326416710607845 2216 32-33 -0.19428922018872896 2216 34-35 -0.282288526541457 2216 36-37 -0.28524948676701456 2216 38-39 -0.2800673142136958 2216 40-41 -0.24831876263838382 2216 42-43 -0.29743748075397747 2216 44-45 -0.28608434766318425 2216 46-47 -0.30494043332978293 2216 48-49 -0.34173862832582813 2216 50-51 -0.38907303482098854 2216 52-53 -0.34609439802049025 2216 54-55 -0.23168872412365715 2216 56-57 -0.21018632166679652 2216 58-59 -0.21010169832089076 2216 60-61 -0.22176152344317757 2216 62-63 -0.3340067713720245 2216 64-65 -0.3663982524932692 2216 66-67 -0.35764448703196194 2216 68-69 -0.29656115016441476 2216 70-71 -0.26074469699914715 2216 72-73 -0.27492115756472657 2216 74-75 -0.3364690345995385 2216 76-77 -0.33118017600184047 2216 78-79 -0.34077315390995366 2216 80-81 -0.2823248942826311 2216 82-83 -0.3594258815718234 2216 84-85 -0.2829192230392721 2216 86-87 -0.3165670670324836 2216 88-89 -0.4490321387708178 2216 90-91 -0.46811938033177825 2216 92-93 -0.4580040005358619 2216 94-95 -0.521487689384184 2216 96-97 -0.5761097208728536 2216 98-99 -0.5255265901509389 2216 100 -0.5910020703227445 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 304.0 3 90.0 4 176.0 5 439.0 6 1645.0 7 3480.0 8 13596.0 9 19353.0 10 2845.0 11 5592.0 12 4558.0 13 6306.0 14 6197.0 15 6715.0 16 8196.0 17 10311.0 18 13836.0 19 23990.0 20 52895.0 21 70007.0 22 31644.0 23 17624.0 24 16216.0 25 17359.0 26 17635.0 27 18838.0 28 21622.0 29 25407.0 30 29502.0 31 35617.0 32 45082.0 33 62582.0 34 95338.0 35 244585.0 36 300115.0 37 535047.0 38 686530.0 39 252639.0 40 2657.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 13.368905483790758 44.961244044493476 24.961006880354027 16.70884359136174 2 15.8314191307445 43.405321144509024 23.796472448229704 16.96678727651677 3 17.026681120448924 25.35881366665706 20.09031397073331 37.52419124216071 4 34.670136361520235 26.345418147986944 21.81030577701976 17.174139713473064 5 15.93922046694542 42.46970980603118 22.543301131432454 19.04776859559095 6 37.457335298920775 23.417905319278645 23.569388561906766 15.555370819893813 7 17.778960086012923 23.56868656639215 41.78373365551233 16.868619692082596 8 36.731619725335015 23.909523862305427 22.85508965221664 16.503766760142913 9 36.26091325921739 25.61326697628364 22.77657699597646 15.349242768522522 10-11 15.509925847105377 45.3833634452462 22.970124548783147 16.136586158865278 12-13 15.386356162966411 24.952116516476575 32.93408262117736 26.72744469937966 14-15 15.68894231453109 34.79058607670866 22.91647846689836 26.603993141861892 16-17 26.227134796859048 24.47118473609947 32.9268346751921 16.374845791849385 18-19 16.080648200489918 24.78047861315244 33.13428065780674 26.004592528550894 20-21 26.33885331585974 35.00971066414366 23.021561867913167 15.629874152083428 22-23 25.86668873692596 25.059172187926286 32.89862804765403 16.17551102749373 24-25 15.766597575529175 35.028707921834645 22.642514326250566 26.56218017638561 26-27 16.250660429990727 29.652401378866976 37.90809031357031 16.188847877571984 28-29 26.3449125646113 24.78167939495376 32.78178654163757 16.09162149879737 30-31 22.8929419893075 26.744495800958408 33.37251576718873 16.990046442545363 32-33 18.316304088995313 28.23226966830152 34.54411571548412 18.90731052721905 34-35 18.748458564267732 27.925042301153173 34.654745183469416 18.671753951109675 36-37 19.01868416482855 27.63820998533199 35.239676047543576 18.103429802295896 38-39 19.81442170366034 27.54262642355176 34.6153327472279 18.027619125560005 40-41 17.7375792977828 29.547249840203655 34.529090324654454 18.186080537359093 42-43 17.867187228668698 27.83553452682776 35.3928526300155 18.904425614488048 44-45 17.942127497003995 28.77327459612783 34.275453871828326 19.00914403503985 46-47 18.977998223926086 27.74776498998811 34.92795114337299 18.34628564271282 48-49 18.026542679148676 28.14836381478872 34.834707736054085 18.990385770008526 50-51 18.943092044251234 29.159027779317253 33.70746331301962 18.19041686341189 52-53 19.12586890675329 28.37893608201105 33.825606064076865 18.669588947158793 54-55 18.843680868260016 29.733324096524647 31.531490590001155 19.891504445214178 56-57 19.22829021250764 30.495815447016444 30.587112097766855 19.68878224270906 58-59 20.98895660323227 30.3815799415455 29.138816784987675 19.490646670234554 60-61 20.21878063226284 31.137804863179436 28.862708715053127 19.780705789504605 62-63 19.14476821077052 31.15283230826385 28.832695470586422 20.86970401037921 64-65 19.834435012389388 31.06642569573337 29.333962178056534 19.76517711382071 66-67 19.835869541723806 30.098521503166463 30.263158137810397 19.802450817299334 68-69 20.510510366272243 30.26257257351197 29.6374076966902 19.58950936352559 70-71 18.47475503041833 31.81963706390094 29.758994010551227 19.946613895129502 72-73 18.58527452206531 30.350951897560286 30.34420903967849 20.719564540695917 74-75 18.852055020252592 31.188051136365736 29.21026894955861 20.74962489382306 76-77 19.892367737958246 30.24721132830389 29.62765429763308 20.232766636104785 78-79 19.46698446432055 30.69554753220343 29.01669065790174 20.82077734557428 80-81 20.2069203004174 31.54875981941259 28.385616705850097 19.858703174319906 82-83 19.895539237628192 31.18670832584383 28.858463296578897 20.059289139949083 84-85 19.533272886886817 31.824906300618355 27.822619976589934 20.819200835904894 86-87 19.472874719066354 32.023618286805885 28.387651020598568 20.115855973529193 88-89 21.412922573518937 31.02745560542938 27.69441970458857 19.86520211646311 90-91 20.332938781097777 32.02935882459767 27.65398575658424 19.983716637720313 92-93 19.37959519088725 32.24016225471685 27.51677343976896 20.86346911462694 94-95 19.92756447940018 32.440150354325866 27.35909639607051 20.273188770203447 96-97 19.962841253044527 31.411814390266972 27.99856345621793 20.62678090047057 98-99 20.89729310526698 31.56647859831967 26.927272582842043 20.608955713571312 100 19.485543520189413 32.99470010360366 26.951140748754465 20.56861562745246 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 118240.0 1 65917.0 2 9344.5 3 4162.5 4 3059.0 5 2777.5 6 2720.5 7 3027.5 8 2968.5 9 2674.0 10 2736.0 11 3637.0 12 28145.5 13 30846.5 14 8528.0 15 7362.0 16 8060.5 17 9013.0 18 10067.5 19 11254.0 20 12679.0 21 14396.5 22 16381.0 23 18909.0 24 21661.5 25 24705.5 26 28669.0 27 32500.0 28 35565.0 29 38386.0 30 41204.5 31 44463.0 32 48171.0 33 52665.5 34 58114.0 35 62295.5 36 64898.5 37 67458.0 38 69676.0 39 71139.0 40 72050.0 41 72648.0 42 72079.5 43 70823.0 44 70681.0 45 71116.0 46 72358.5 47 76924.0 48 79220.5 49 88473.5 50 130186.5 51 129274.0 52 82716.0 53 67375.0 54 65258.0 55 64874.0 56 60105.5 57 51187.5 58 44805.5 59 37048.0 60 29253.5 61 24111.0 62 19544.0 63 14558.0 64 10376.0 65 7831.0 66 5835.0 67 4322.0 68 3305.5 69 2354.0 70 1735.0 71 1349.0 72 1013.0 73 759.5 74 559.0 75 397.5 76 268.0 77 174.0 78 113.5 79 70.0 80 37.5 81 16.5 82 9.5 83 8.0 84 4.0 85 2.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.29620516003650377 2 0.0017365152203711709 3 4.4336558817987344E-4 4 0.003251347646652405 5 3.694713234832279E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 1.6626209556745253E-4 16-17 3.694713234832279E-5 18-19 0.0 20-21 3.5099775730906645E-4 22-23 0.0022907222055960127 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 1.8473566174161394E-5 34-35 0.0030850855510849524 36-37 0.0 38-39 1.2931496321912976E-4 40-41 0.0 42-43 2.955770587865823E-4 44-45 0.0019027773159386234 46-47 0.0014224645954104271 48-49 0.0022537750732476896 50-51 8.867311763597469E-4 52-53 2.955770587865823E-4 54-55 2.771034926124209E-4 56-57 3.140506249607437E-4 58-59 7.574162131406171E-4 60-61 0.002549352132034272 62-63 2.0320922791577532E-4 64-65 7.204690807922944E-4 66-67 1.2931496321912976E-4 68-69 1.4778852939329116E-4 70-71 0.0022353015070735284 72-73 9.236783087080696E-5 74-75 1.1084139704496836E-4 76-77 0.00145941172775875 78-79 9.606254410563925E-4 80-81 0.002549352132034272 82-83 1.2931496321912976E-4 84-85 0.0015517795586295572 86-87 0.007112322977052136 88-89 0.0027156142276017247 90-91 0.004673812242062832 92-93 0.00768500352845114 94-95 0.006022382572776614 96-97 0.0027340877937758862 98-99 6.835219484439716E-4 100 0.003768607499528924 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2706570.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.1726994968345 #Duplication Level Percentage of deduplicated Percentage of total 1 84.98106731499169 46.886348898903 2 9.910777691796316 10.936087187388182 3 2.3117132609743805 3.826303832117605 4 0.8580504546318236 1.8936383954609528 5 0.4632127127544791 1.2778347901958196 6 0.2886091922473059 0.9554008941530847 7 0.20249649253390187 0.7820594692215169 8 0.14780803462667585 0.6523974622140235 9 0.10508651075947667 0.5218115831373124 >10 0.6499220198479015 7.609659712702713 >50 0.04472330181856698 1.6826726993672567 >100 0.029713584146595667 3.0126359352900525 >500 0.00220628581120082 0.8761743490266587 >1k 0.004145143039225039 4.488913452213887 >5k 1.3371429158790448E-4 0.42991672790402863 >10k+ 3.342857289697612E-4 14.168144610703875 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 212871 7.864973010119819 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 112661 4.162500877494393 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 35192 1.3002434816021753 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 11748 0.43405491082809605 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11625 0.42951041354925235 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6350 0.2346142904118497 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 5305 0.1960045371078524 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC 4936 0.18237104527132128 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4798 0.17727234100725273 No Hit GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT 4065 0.1501900929959321 No Hit CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 2996 0.11069360851557507 No Hit AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC 2819 0.10415396608992192 No Hit TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG 2720 0.10049619998743796 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.020247028526880886 0.0 0.0 0.0 0.0 2 0.025641309849736012 0.0 0.0 0.0 0.0 3 0.030592225584411267 0.0 0.0 0.0 0.0 4 0.03140506249607437 0.0 0.0 0.0 0.0 5 0.03151590389311933 0.0 0.0 0.0 0.0 6 0.031552851025467656 0.0 0.0 0.0 0.0 7 0.03170063955486095 0.0 0.0 0.0 0.0 8 0.03188537521660256 0.0 0.0 0.0 0.0 9 0.03240263506947908 0.0 0.0 0.0 0.0 10-11 0.03256889716504653 0.0 0.0 0.0 0.0 12-13 0.03260584429739486 0.0 0.0 0.0 0.0 14-15 0.032661264995917344 0.0 0.0 0.0 0.0 16-17 0.03280905352531063 0.0 0.0 0.0 0.0 18-19 0.03297531562087808 0.0 0.0 0.0 0.0 20-21 0.033012262753226405 0.0 0.0 0.0 0.0 22-23 0.03314157771644554 0.0 0.0 0.0 0.0 24-25 0.033215471981142185 0.0 0.0 0.0 0.0 26-27 0.033400207642883796 0.0 0.0 0.0 0.0 28-29 0.03347410190758044 0.0 0.0 0.0 0.0 30-31 0.03351104903992877 0.0 0.0 0.0 0.0 32-33 0.03367731113549622 0.0 0.0 0.0 1.847356617416139E-5 34-35 0.03388052036341199 0.0 0.0 0.0 3.694713234832278E-5 36-37 0.034323885951591866 0.0 0.0 0.0 3.694713234832278E-5 38-39 0.035284511392648266 0.0 0.0 0.0 3.694713234832278E-5 40-41 0.03672544955423285 0.0 0.0 0.0 3.694713234832278E-5 42-43 0.0384250176422557 0.0 0.0 0.0 3.694713234832278E-5 44-45 0.03997679720088525 0.0 0.0 0.0 3.694713234832278E-5 46-47 0.04204583661239133 0.0 0.0 0.0 3.694713234832278E-5 48-49 0.04463213587677392 0.0 0.0 0.0 3.694713234832278E-5 50-51 0.04777264212638136 0.0 0.0 0.0 3.694713234832278E-5 52-53 0.05290829352279823 0.0 0.0 0.0 3.694713234832278E-5 54-55 0.05913388532349062 0.0 0.0 0.0 3.694713234832278E-5 56-57 0.06558115991827294 0.0 0.0 0.0 3.694713234832278E-5 58-59 0.07361716120403314 0.0 0.0 0.0 3.694713234832278E-5 60-61 0.08411014679095682 0.0 0.0 0.0 3.694713234832278E-5 62-63 0.0972817994731339 0.0 0.0 0.0 3.694713234832278E-5 64-65 0.11411121825779492 0.0 0.0 0.0 3.694713234832278E-5 66-67 0.134949400902249 0.0 0.0 0.0 3.694713234832278E-5 68-69 0.16175454542095716 0.0 0.0 0.0 3.694713234832278E-5 70-71 0.19846152140901585 0.0 0.0 0.0 3.694713234832278E-5 72-73 0.24069209368314878 0.0 0.0 0.0 3.694713234832278E-5 74-75 0.290736984448952 0.0 0.0 0.0 3.694713234832278E-5 76-77 0.3529374817573534 0.0 0.0 0.0 3.694713234832278E-5 78-79 0.4283465788802802 0.0 0.0 0.0 3.694713234832278E-5 80-81 0.5224509249714584 0.0 0.0 0.0 3.694713234832278E-5 82-83 0.6407371691846138 0.0 0.0 0.0 3.694713234832278E-5 84-85 0.7824294217404316 0.0 0.0 0.0 3.694713234832278E-5 86-87 0.9496706163151147 0.0 0.0 0.0 3.694713234832278E-5 88 1.0922311264811182 0.0 0.0 0.0 3.694713234832278E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 3350 0.0 85.26551 1 GTGGAAT 85355 0.0 60.598305 6 TGGAATC 85995 0.0 60.130924 7 AACGAGT 79350 0.0 59.989742 1 ACGAGTG 84370 0.0 59.869728 2 GAATCAC 86410 0.0 59.847576 9 GGAATCA 86525 0.0 59.77889 8 AGTGGAA 86210 0.0 59.37696 5 CGAGTGG 86130 0.0 59.080715 3 GAGTGGA 88015 0.0 58.21055 4 AACGTGT 955 0.0 55.772564 1 CGTGTGG 1090 0.0 51.742428 3 ATCGAGT 540 0.0 48.008095 1 ACGTGTG 1265 0.0 43.471382 2 ATCTGCG 1490 0.0 41.323887 94 TCGAGTG 650 0.0 41.216354 2 AGTACTT 32245 0.0 41.123844 20-21 GAGTACT 31865 0.0 40.670322 20-21 GTACTTT 31880 0.0 39.935455 22-23 TACTTTT 33930 0.0 38.526825 22-23 >>END_MODULE