FastQCFastQC Report
Thu 26 May 2016
SRR2132647_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132647_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5622936
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2865925.096839089045296No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA487210.8664690474869357No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG199250.35435224587297454No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158190.2813298959831661No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT67200.11951051905979367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT42150.083.514761
AACGAGT802300.076.245041
ACGAGTG862400.075.040042
CGAGTGG882900.073.7654953
GTGGAAT902500.073.247356
GAATCAC906050.072.975929
TGGAATC908550.072.764787
GGAATCA909950.072.704478
AGTGGAA912750.071.714255
GAGTGGA914400.071.599574
GAGTACT425350.041.5791520-21
AGTACTT436250.041.3859820-21
GTACTTT431050.040.40148522-23
AACGTGT16450.038.3865551
CATGGGG224500.037.78586624-25
TACTTTT476500.037.4454222-23
TGAGTAC910400.037.08185618-19
ATCACTG928100.036.9047410-11
GCTGAGT935300.036.5745316-17
CACTGCT945000.036.2845312-13
Produced by FastQC (version 0.11.5)