FastQCFastQC Report
Thu 26 May 2016
SRR2132646_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132646_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3384627
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2347296.935151199822019No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA706312.0868178384206No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG560281.655367046354No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT126110.37259644858946056No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71020.20983109807964068No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT60590.17901529474296576No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54000.15954490701634183No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT50410.14893812523507022No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC44210.1306200062813421No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA43820.12946773750844628No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG42460.12544956947988656No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT41940.12391321111602548No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41700.12320412264039729No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG40680.1201904966189775No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT39730.11738368806961594No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC39370.11632005535617367No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA37590.11106098249526461No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT36990.10928826130619416No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC36450.10769281223603075No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT36400.10754508547027486No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT36200.10695417840725138No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA36040.10648145275683259No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG35940.10618599922532085No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA35700.10547691074969266No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG34310.10137010666167942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT37250.089.451361
GTGGAAT1114100.056.0154536
AGTGGAA1113800.055.4988825
TGGAATC1128800.055.3026357
GAATCAC1136900.054.9416969
GGAATCA1136750.054.9200068
ACGAGTG1111700.054.7978972
AACGAGT1053450.054.612091
CGAGTGG1136650.053.9829333
GAGTGGA1164900.053.089344
ACGTGTG12300.050.436892
AACGTGT11800.049.5260241
ATCGAGT7200.045.165871
CGTGTGG14400.043.7336163
AGTACTT362850.039.33808520-21
GAGTACT358550.038.8660620-21
GTACTTT360850.037.92011322-23
TCGAGTG9550.037.401562
TACTTTT377050.037.27557422-23
CATGGGG184400.035.80816724-25
Produced by FastQC (version 0.11.5)