##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132646_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3384627
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.446115332649654	31.0	30.0	34.0	16.0	34.0
2	31.04866090118645	33.0	31.0	34.0	25.0	34.0
3	31.837505875832107	34.0	31.0	34.0	28.0	34.0
4	35.922301925736576	37.0	35.0	37.0	35.0	37.0
5	35.653673211257846	37.0	35.0	37.0	35.0	37.0
6	36.11086096045443	37.0	36.0	37.0	35.0	37.0
7	36.159802247042286	37.0	35.0	37.0	35.0	37.0
8	36.30930941577905	37.0	37.0	37.0	35.0	37.0
9	38.22263753140302	39.0	39.0	39.0	37.0	39.0
10-11	38.26399245766225	39.0	39.0	39.0	37.0	39.0
12-13	38.23319881334044	39.0	39.0	39.0	37.0	39.0
14-15	39.76449192185727	41.0	40.0	41.0	37.5	41.0
16-17	39.666740530049545	41.0	40.0	41.0	37.0	41.0
18-19	39.55354563442294	41.0	39.5	41.0	36.5	41.0
20-21	39.48880304978954	41.0	39.5	41.0	36.0	41.0
22-23	39.172306579129696	40.5	39.0	41.0	36.0	41.0
24-25	39.058801752748536	41.0	39.0	41.0	35.0	41.0
26-27	39.01374641873388	41.0	39.0	41.0	35.0	41.0
28-29	38.896630559290585	41.0	39.0	41.0	35.0	41.0
30-31	38.728101649014796	41.0	39.0	41.0	35.0	41.0
32-33	38.59164732184669	41.0	38.5	41.0	35.0	41.0
34-35	38.49631983081149	41.0	38.0	41.0	35.0	41.0
36-37	38.32449557957199	40.5	38.0	41.0	35.0	41.0
38-39	37.91746815823427	40.0	36.5	41.0	34.5	41.0
40-41	37.735807963477214	40.0	36.0	41.0	34.0	41.0
42-43	37.60811664032698	40.0	35.5	41.0	33.5	41.0
44-45	37.431545928103745	40.0	35.0	41.0	33.0	41.0
46-47	37.30307239172883	40.0	35.0	41.0	33.0	41.0
48-49	37.133211872386525	40.0	35.0	41.0	33.0	41.0
50-51	36.86947882292495	40.0	35.0	41.0	33.0	41.0
52-53	36.355353337310135	39.5	35.0	41.0	31.5	41.0
54-55	35.40484446292014	39.0	35.0	41.0	26.5	41.0
56-57	34.8020453952533	39.0	35.0	41.0	22.0	41.0
58-59	34.45953128660854	39.0	35.0	41.0	19.0	41.0
60-61	34.18253621447799	38.5	35.0	41.0	16.5	41.0
62-63	33.90095836262016	38.0	35.0	40.0	12.5	41.0
64-65	33.56872529823818	37.0	34.5	40.0	9.0	41.0
66-67	33.17327714398071	37.0	34.0	40.0	7.0	41.0
68-69	32.84751451193883	36.0	34.0	39.0	3.5	41.0
70-71	32.505832104985274	36.0	34.0	39.0	2.0	41.0
72-73	32.153727574707645	35.0	34.0	38.5	2.0	40.5
74-75	31.948318825087668	35.0	34.0	37.5	2.0	40.0
76-77	31.629571884878303	35.0	34.0	37.0	2.0	39.5
78-79	31.275227521378277	35.0	33.0	37.0	2.0	39.0
80-81	30.956188377626248	35.0	33.0	36.0	2.0	39.0
82-83	30.610354109921126	35.0	33.0	36.0	2.0	37.0
84-85	30.266930299852838	35.0	33.0	35.5	2.0	37.0
86-87	30.08492250401595	35.0	33.0	35.0	2.0	37.0
88-89	29.869751526534536	35.0	33.0	35.0	2.0	36.0
90-91	29.664154129834692	35.0	32.0	35.0	2.0	36.0
92-93	29.5089315602576	35.0	32.0	35.0	2.0	36.0
94-95	29.29925468892141	35.0	32.0	35.0	2.0	35.5
96-97	29.06210285505611	35.0	32.0	35.0	2.0	35.0
98-99	28.918637267858465	35.0	32.0	35.0	2.0	35.0
100	28.840368229645392	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.3616970709397087
1101	2	2.011938742694756
1101	3	1.319293463252631
1101	4	0.5775506819765184
1101	5	0.8287554182760672
1101	6	0.40113356783998455
1101	7	0.27299782340016776
1101	8	0.20750696579091965
1101	9	0.1842532937419108
1101	10-11	0.1277208722361749
1101	12-13	0.18742397652749077
1101	14-15	0.24775617838253794
1101	16-17	0.21126754215212884
1101	18-19	0.15277963609599254
1101	20-21	0.2227610719691313
1101	22-23	0.1734192191162407
1101	24-25	0.23901147009022594
1101	26-27	0.23384931664804753
1101	28-29	0.1614788014643409
1101	30-31	0.17125945095897777
1101	32-33	0.2498493460025344
1101	34-35	0.21551169199243958
1101	36-37	0.2769379293594696
1101	38-39	0.16265093224961902
1101	40-41	0.18730494845745227
1101	42-43	0.2217219507796102
1101	44-45	0.24263816299797014
1101	46-47	0.2079822261065445
1101	48-49	0.2630535107239851
1101	50-51	0.26062520395212374
1101	52-53	0.27841756833635856
1101	54-55	0.3298394352200802
1101	56-57	0.18857363194688048
1101	58-59	0.09388393373168213
1101	60-61	0.10526134264703302
1101	62-63	0.12205589916273141
1101	64-65	0.1624935557742262
1101	66-67	0.12165003418491693
1101	68-69	0.022765618492620376
1101	70-71	0.04881381408581831
1101	72-73	0.04791876695905728
1101	74-75	0.03054908332130779
1101	76-77	0.032602265661491714
1101	78-79	-0.07247121866709705
1101	80-81	-0.008803314456741873
1101	82-83	-0.0018677438122303158
1101	84-85	0.0898354033825477
1101	86-87	-0.032540532342824946
1101	88-89	0.05412478629761708
1101	90-91	0.10200436466319118
1101	92-93	0.05745324109665262
1101	94-95	0.0564224089730736
1101	96-97	0.06612960622305053
1101	98-99	0.11805550581926383
1101	100	0.14740254875935221
1102	1	3.4166172441478544
1102	2	2.056145691028032
1102	3	1.3339952244659194
1102	4	0.63290741533865
1102	5	0.9298704979159709
1102	6	0.4835689473291751
1102	7	0.3223518106722949
1102	8	0.27178890942461464
1102	9	0.2508430083266333
1102	10-11	0.26476015187673596
1102	12-13	0.25319109534259354
1102	14-15	0.274331623852639
1102	16-17	0.3793716517230976
1102	18-19	0.2563481459848802
1102	20-21	0.28024260621775454
1102	22-23	0.2903044406887716
1102	24-25	0.3266729073132666
1102	26-27	0.29298439723336855
1102	28-29	0.3126584439009932
1102	30-31	0.37364602084191034
1102	32-33	0.4174222663450422
1102	34-35	0.4114666948121837
1102	36-37	0.37866148010441947
1102	38-39	0.3569206624885979
1102	40-41	0.386311980778423
1102	42-43	0.31700542575973856
1102	44-45	0.3777121963973755
1102	46-47	0.35367111763378745
1102	48-49	0.34887629887379745
1102	50-51	0.35422085593963004
1102	52-53	0.29789000684889544
1102	54-55	0.34631054466023414
1102	56-57	0.27140595226101993
1102	58-59	0.14563313150622292
1102	60-61	0.18802141594939314
1102	62-63	0.20364636677854264
1102	64-65	0.22088268486353968
1102	66-67	0.21549622016490133
1102	68-69	0.2789839733230579
1102	70-71	0.2986117269255004
1102	72-73	0.22512217439724935
1102	74-75	0.2659552858789773
1102	76-77	0.30647481322612435
1102	78-79	0.2666158658054023
1102	80-81	0.2539154905433847
1102	82-83	0.3004495691969602
1102	84-85	0.23414085038070098
1102	86-87	0.28790519610207
1102	88-89	0.32815651474031426
1102	90-91	0.37342983602217217
1102	92-93	0.3078945612865702
1102	94-95	0.3795498954872194
1102	96-97	0.3356121694309415
1102	98-99	0.36825466880047486
1102	100	0.37538914150804814
1103	1	3.3743900011819825
1103	2	2.0773164665182016
1103	3	1.3181313565971031
1103	4	0.5992617739425441
1103	5	0.894161033007336
1103	6	0.45161541481854073
1103	7	0.3086170363631311
1103	8	0.2175717980103542
1103	9	0.19624768945340776
1103	10-11	0.11370495135758318
1103	12-13	0.16765130265530814
1103	14-15	0.17702936133992608
1103	16-17	0.24157795359741385
1103	18-19	0.17579557437920101
1103	20-21	0.19777123191761348
1103	22-23	0.16654480652226056
1103	24-25	0.1482708598841569
1103	26-27	0.20107088619692348
1103	28-29	0.2032437607375286
1103	30-31	0.19175565675123352
1103	32-33	0.17012272911308912
1103	34-35	0.2219954795864254
1103	36-37	0.2593137038651818
1103	38-39	0.28652557110369514
1103	40-41	0.2934572867685077
1103	42-43	0.3167880013226281
1103	44-45	0.35463323313860684
1103	46-47	0.25454857687930854
1103	48-49	0.3077430340113665
1103	50-51	0.36914663774493306
1103	52-53	0.4744547750684589
1103	54-55	0.44236323604157946
1103	56-57	0.4856177831234305
1103	58-59	0.43614590376092366
1103	60-61	0.39366283683389014
1103	62-63	0.4770949942964009
1103	64-65	0.49621331524233625
1103	66-67	0.5012285594912314
1103	68-69	0.5319573913274809
1103	70-71	0.5400786080150937
1103	72-73	0.5366399840643226
1103	74-75	0.5050857940216709
1103	76-77	0.5380099254359259
1103	78-79	0.5714599199433223
1103	80-81	0.5283544671500522
1103	82-83	0.550714536541431
1103	84-85	0.515160739291769
1103	86-87	0.4937867876933737
1103	88-89	0.5087102952087825
1103	90-91	0.5708280514844084
1103	92-93	0.48602749791926314
1103	94-95	0.5429235359223874
1103	96-97	0.5885653614096285
1103	98-99	0.5880355340147503
1103	100	0.667084783860993
1104	1	2.4639959260548245
1104	2	1.7933419234426076
1104	3	1.2291023857616743
1104	4	0.5105398695405654
1104	5	0.8541260849076195
1104	6	0.3677728906966422
1104	7	0.21837472248922296
1104	8	0.20392426832186317
1104	9	0.18855917573339553
1104	10-11	0.187654026934986
1104	12-13	0.18798509520971862
1104	14-15	0.22769080341463166
1104	16-17	0.2329632094969014
1104	18-19	0.2445526605276669
1104	20-21	0.20651895855157676
1104	22-23	0.1920427896560284
1104	24-25	0.25125579120223307
1104	26-27	0.292067009365951
1104	28-29	0.2951214314736248
1104	30-31	0.31960273647355564
1104	32-33	0.32973476973283766
1104	34-35	0.2701564748329943
1104	36-37	0.3508658040256236
1104	38-39	0.35407579492706986
1104	40-41	0.3656049798391905
1104	42-43	0.38210932873646186
1104	44-45	0.4016555414595686
1104	46-47	0.4182294396055184
1104	48-49	0.3776815712665993
1104	50-51	0.4699867940188085
1104	52-53	0.5335736760349761
1104	54-55	0.43361384659300484
1104	56-57	0.4275686262301406
1104	58-59	0.34322215208275253
1104	60-61	0.43261384298170213
1104	62-63	0.2858268431825337
1104	64-65	0.45597703866459227
1104	66-67	0.43361301689861875
1104	68-69	0.35044429141807143
1104	70-71	0.3349889285852825
1104	72-73	0.2724563032510616
1104	74-75	0.29825079878328964
1104	76-77	0.28391351766125794
1104	78-79	0.3927568914718407
1104	80-81	0.35308246478697924
1104	82-83	0.44632346096130604
1104	84-85	0.36637353658596794
1104	86-87	0.37765589979470704
1104	88-89	0.4516181094483436
1104	90-91	0.44388430646432653
1104	92-93	0.40999176053687236
1104	94-95	0.5009902077963666
1104	96-97	0.5680040617059703
1104	98-99	0.6242692878895362
1104	100	0.6606798777562979
1105	1	3.3115530449999575
1105	2	1.9989559227523195
1105	3	1.2942839746326875
1105	4	0.5740647766616291
1105	5	0.8765966629819175
1105	6	0.38606978671919734
1105	7	0.21363299538926128
1105	8	0.16010270031064522
1105	9	0.1971898645169574
1105	10-11	0.13191792133820712
1105	12-13	0.09088625455033394
1105	14-15	0.15859997433322093
1105	16-17	0.08438219735704422
1105	18-19	0.07841417969965647
1105	20-21	0.1030320946035701
1105	22-23	0.06610443572070324
1105	24-25	0.08504013703338131
1105	26-27	0.10501151549433985
1105	28-29	0.11228300913315792
1105	30-31	0.19874025378419446
1105	32-33	0.21775091570933114
1105	34-35	0.18610834395488496
1105	36-37	0.0720645490229046
1105	38-39	0.11806937410511864
1105	40-41	0.07775543796969941
1105	42-43	0.12855451119886396
1105	44-45	0.15713287827653488
1105	46-47	0.13610611857722432
1105	48-49	0.12328613900886154
1105	50-51	0.08374230986019882
1105	52-53	-0.005993383164856425
1105	54-55	0.004661225113125056
1105	56-57	-0.05096532166570711
1105	58-59	-0.06864839669851364
1105	60-61	-0.1384719580102498
1105	62-63	-0.09258290489412246
1105	64-65	-0.0028599738667125507
1105	66-67	-0.07801905804949882
1105	68-69	-0.0114287285520831
1105	70-71	0.01436120063083024
1105	72-73	0.0589084740057757
1105	74-75	0.015003456255495706
1105	76-77	-0.04485899437696261
1105	78-79	-0.02403395038422218
1105	80-81	-0.02390027432804942
1105	82-83	-0.030166830139819467
1105	84-85	0.0046413579628499235
1105	86-87	0.007849255137220013
1105	88-89	0.013315363029761329
1105	90-91	0.025169919087652204
1105	92-93	0.0012554756352294305
1105	94-95	-0.004822422556795658
1105	96-97	0.011634765267402258
1105	98-99	0.032823066050006844
1105	100	-0.033720753029033546
1106	1	1.8141185301267484
1106	2	1.4093927800300996
1106	3	1.0300943361900003
1106	4	0.44735678311792526
1106	5	0.6958673458034212
1106	6	0.23621874203494997
1106	7	0.1315097477874616
1106	8	0.12817140301321928
1106	9	0.14668872620701023
1106	10-11	0.10161062464224102
1106	12-13	0.03173936440369829
1106	14-15	0.10265797292824885
1106	16-17	0.10658288292741247
1106	18-19	0.11898282953954009
1106	20-21	0.17151870624605436
1106	22-23	0.062490859641890495
1106	24-25	0.033705706505529065
1106	26-27	0.08445550571121885
1106	28-29	0.10326486900708431
1106	30-31	0.13259962962852967
1106	32-33	0.08639370723533091
1106	34-35	0.10477833039853834
1106	36-37	0.13719667050842332
1106	38-39	0.07381825047924906
1106	40-41	0.06232041326519777
1106	42-43	0.08161267660504734
1106	44-45	0.08970393160677048
1106	46-47	0.0904258227341117
1106	48-49	0.14824026594040873
1106	50-51	0.15051828482442886
1106	52-53	0.019506228000686576
1106	54-55	0.05682419021670171
1106	56-57	0.16043710202993822
1106	58-59	0.11230793636933356
1106	60-61	0.0820597349350436
1106	62-63	0.1324378877643042
1106	64-65	0.05188045857358503
1106	66-67	-0.027547522846468553
1106	68-69	0.04740380479251627
1106	70-71	0.04350522444488547
1106	72-73	0.0017862249214743997
1106	74-75	0.057831516241723335
1106	76-77	0.026579939817207077
1106	78-79	0.010016024829049286
1106	80-81	0.01607226056720279
1106	82-83	-0.06753053250172414
1106	84-85	-0.08822435200658063
1106	86-87	-0.09747231448529092
1106	88-89	-0.01730347112470554
1106	90-91	0.02107270812322426
1106	92-93	0.018635788629350003
1106	94-95	0.056407022666885354
1106	96-97	0.11661396086170583
1106	98-99	0.0576513797771554
1106	100	0.03995903063334083
1107	1	2.9070440699047104
1107	2	1.8816559349509028
1107	3	1.256689726880861
1107	4	0.5346463383451123
1107	5	0.8427924278352918
1107	6	0.36195851280803737
1107	7	0.19102906872512193
1107	8	0.14967325540094834
1107	9	0.12181393574571331
1107	10-11	0.11913356759577454
1107	12-13	0.10052484757411406
1107	14-15	0.13281539306667867
1107	16-17	0.20209691410094877
1107	18-19	0.16067071405054634
1107	20-21	0.19281691298861858
1107	22-23	0.10004082217177768
1107	24-25	0.13518790206585152
1107	26-27	0.17981035311110816
1107	28-29	0.1629180892394615
1107	30-31	0.2381320790895387
1107	32-33	0.18572095745406614
1107	34-35	0.17407954844835416
1107	36-37	0.21062981760415767
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2216	84-85	-0.22099149623411662
2216	86-87	-0.21421584960357976
2216	88-89	-0.2883684763038765
2216	90-91	-0.2969097732014241
2216	92-93	-0.34610287323348743
2216	94-95	-0.36485694187662787
2216	96-97	-0.43664630608057564
2216	98-99	-0.5092424373002622
2216	100	-0.5100481550016198
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	421.0
3	97.0
4	152.0
5	405.0
6	1307.0
7	2595.0
8	9417.0
9	12946.0
10	2826.0
11	6486.0
12	5272.0
13	7819.0
14	7754.0
15	8488.0
16	10950.0
17	14239.0
18	18986.0
19	29309.0
20	61097.0
21	83609.0
22	39134.0
23	22432.0
24	21525.0
25	22364.0
26	23730.0
27	25410.0
28	29488.0
29	34189.0
30	39409.0
31	47345.0
32	60027.0
33	80384.0
34	119135.0
35	244815.0
36	371764.0
37	705704.0
38	905557.0
39	304186.0
40	3854.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.990867758517433	42.81147868644835	24.795615054300654	18.40203850073356
2	16.864736364863855	41.113129741536845	24.03120266218911	17.990931231410194
3	18.08938669629087	24.13517617930521	19.350414522162016	38.4250226022419
4	34.9732546375962	25.049312869920392	21.61218515401925	18.365247338464158
5	16.996629463125004	40.28991108022634	22.408427051276597	20.30503240537206
6	38.128160060177976	21.840929591355266	23.245958860459364	16.784951488007394
7	19.02318926132776	22.083024215075987	40.793003187648154	18.100783335948098
8	37.05477738019581	22.546236261780102	22.606922417152614	17.792063940871476
9	36.77663742563065	24.100971835301202	22.738015149084376	16.384375589983772
10-11	16.6668882568153	43.10525207061221	22.794594500368873	17.433265172203612
12-13	16.52377056615101	23.464949018015872	32.312452745900806	27.698827669932314
14-15	16.98101250021605	32.95485182021092	22.56280355129813	27.501332128274903
16-17	27.05991740298633	22.914333814134853	32.46649703689566	17.559251745983158
18-19	17.25264220439094	23.342989248592126	32.51276027059411	26.89160827642282
20-21	27.144171070413265	33.10487328259792	22.95505726200121	16.795898384987613
22-23	26.581302896852893	23.507645806587906	32.49215918878073	17.41889210777847
24-25	16.8492126311112	33.09055916649014	22.734262889234174	27.32596531316449
26-27	17.377823317015434	28.35844245170886	36.99385486199809	17.269879369277618
28-29	28.067405950493214	23.148813739298305	31.686401485303996	17.09737882490449
30-31	24.12638083901121	25.69599249784393	32.22335873347344	17.95426792967142
32-33	19.32838369734204	26.554263307083264	33.61374343871376	20.503609556860937
34-35	20.03659267127239	26.645565927008185	33.601216702626566	19.716624699092865
36-37	20.16753404475621	26.053325545174793	34.46071064583068	19.318429764238317
38-39	21.405780296952226	26.126578224399587	33.513319152513056	18.95432232613513
40-41	18.843748342690102	28.613748075304617	33.39926872285612	19.143234859149157
42-43	18.857623772000434	26.43203277885955	34.49813649713461	20.212206952005403
44-45	18.72276990186676	27.614207603775	33.109022029899116	20.554000464459126
46-47	20.109585317517475	26.508005799837647	33.991894113814794	19.390514768830084
48-49	19.0027027246626	26.711517566233006	33.89312751556892	20.392652193535472
50-51	20.19032643681421	27.89281824826878	32.63713708478919	19.27971823012782
52-53	20.180295460837304	27.16257496060847	32.99971547690414	19.657414101650087
54-55	19.54989038651311	28.731777865757458	30.507767489407968	21.210564258321465
56-57	20.107964964420635	29.41102780772947	29.9200810490642	20.560926178785692
58-59	22.528864580077162	28.81797188321922	28.24333938624321	20.40982415046041
60-61	21.223495965718396	30.16329618468363	27.991696398537414	20.62151145106056
62-63	20.02007315148035	30.007869419924628	27.947608897363825	22.024448531231197
64-65	20.76494449784812	29.941749368499693	28.350570976143846	20.942735157508345
66-67	20.84037744835317	28.77231194502665	29.607235313130982	20.7800752934892
68-69	21.748563132216656	28.824293074050576	28.801484007505717	20.625659786227047
70-71	19.42903217514549	30.817985982853358	28.86264100392103	20.89034083808012
72-73	19.44716467070424	28.819592838142015	29.748071019399642	21.985171471754107
74-75	19.56584780061688	29.902164778049645	28.340999630829995	22.190987790503485
76-77	20.8961221203899	28.856736013723356	28.98666295179526	21.260478914091482
78-79	20.13219856789809	29.23506151603193	28.4999093690535	22.132830547016482
80-81	21.32718561243033	30.236555085955768	27.482401333939816	20.95385796767409
82-83	21.142838487019137	29.462791427034645	28.376907693973603	21.01746239197262
84-85	20.432673237927293	30.43965636570376	27.02740792708356	22.100262469285394
86-87	20.584478890231154	30.508434762683613	27.8678170963887	21.039269250696538
88-89	22.736444787507896	29.40106292218656	26.942721125116798	20.919771165188745
90-91	21.44941016293721	30.589668647661956	26.793325678816387	21.167595510584448
92-93	20.482815728260476	30.594511463198614	26.450327124239255	22.472345684301658
94-95	21.29129686348597	30.841452309350565	26.29064978794241	21.576601039221053
96-97	21.576517125059425	29.742981548764487	27.149588585519524	21.530912740656564
98-99	22.432202256834803	29.895741234397192	26.163890495426724	21.50816601334128
100	20.41048535542601	31.81871633579847	26.089117248185122	21.681681060590392
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	69724.0
1	39024.0
2	5923.5
3	3012.5
4	2404.5
5	2221.5
6	2251.5
7	2532.0
8	2457.0
9	2308.5
10	2400.5
11	3163.0
12	23398.5
13	26176.0
14	8571.5
15	7962.0
16	8955.0
17	10211.5
18	11570.0
19	13153.0
20	14871.0
21	16983.5
22	19450.0
23	22435.0
24	25568.5
25	28616.5
26	32434.5
27	36946.5
28	40602.5
29	44605.5
30	49486.0
31	53799.0
32	58639.0
33	64733.0
34	70938.0
35	75477.0
36	80004.0
37	85025.5
38	87298.0
39	89137.0
40	91999.0
41	93186.0
42	92424.5
43	91832.0
44	91845.5
45	91942.0
46	93203.0
47	98063.5
48	102509.5
49	119237.5
50	185466.5
51	185627.5
52	113783.0
53	89115.0
54	85218.0
55	82811.0
56	77633.5
57	70780.0
58	64761.0
59	56293.5
60	45878.0
61	36756.5
62	29260.0
63	22658.0
64	16683.5
65	11991.5
66	8912.0
67	6720.5
68	5046.5
69	3719.0
70	2662.0
71	1952.0
72	1491.0
73	1134.0
74	792.0
75	555.0
76	393.5
77	249.0
78	160.5
79	108.0
80	76.0
81	44.0
82	20.0
83	10.0
84	5.5
85	3.0
86	2.0
87	1.0
88	1.5
89	1.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2951580779802324
2	0.0012999955386516742
3	2.0681747205822092E-4
4	0.003397715612385057
5	8.863605945352324E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.6249944233145928E-4
16-17	2.9545353151174413E-5
18-19	1.4772676575587207E-5
20-21	2.659081783605697E-4
22-23	0.002718172489908046
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	2.9545353151174413E-5
34-35	0.002053402044006622
36-37	4.431802972676162E-5
38-39	2.5113550178498253E-4
40-41	1.4772676575587207E-5
42-43	1.920447954826337E-4
44-45	0.0013295408918028485
46-47	0.0014624949809831334
48-49	0.002348855575518366
50-51	6.204524161746627E-4
52-53	4.727256504187906E-4
54-55	2.0681747205822092E-4
56-57	3.1022620808733136E-4
58-59	8.56815241384058E-4
60-61	0.0021124927503089704
62-63	1.920447954826337E-4
64-65	8.420425648084708E-4
66-67	1.4772676575587206E-4
68-69	2.363628252093953E-4
70-71	0.001713630482768116
72-73	7.386338287793603E-5
74-75	2.2159014863380809E-4
76-77	9.602239774131684E-4
78-79	6.352250927502499E-4
80-81	0.0019647659845530986
82-83	3.1022620808733136E-4
84-85	0.0011965868026225636
86-87	0.006219296838322214
88-89	0.0026443091070301097
90-91	0.003929531969106197
92-93	0.00618975148517104
94-95	0.004993164682548476
96-97	0.002481809664698651
98-99	5.761343864479011E-4
100	0.002865899255663918
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3384627.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.44372295343203
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.94280738372802	51.087604319585026
2	9.369847896889246	11.13957284996964
3	2.0752917530163257	3.7008920404153427
4	0.7645059644973247	1.8178032299930127
5	0.4008629319088627	1.1914392533345464
6	0.2580865136293851	0.9204973928521397
7	0.18217723872837882	0.75805053151737
8	0.14016817908672222	0.6665694723614528
9	0.10746210339605067	0.5749152752041113
>10	0.7133003926806041	7.961993732799195
>50	0.024886918063386332	1.0127922777604004
>100	0.015543840765123345	1.808135463012856
>500	0.0011903255556482942	0.49264827601042305
>1k	0.003471782869892587	5.144916695631363
>5k	1.9838759256529066E-4	0.6958398927865692
>10k+	1.9838759256529066E-4	11.02632929676655
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	234729	6.935151199822019	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	70631	2.0868178384206	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	56028	1.655367046354	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12611	0.37259644858946056	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7102	0.20983109807964068	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	6059	0.17901529474296576	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5400	0.15954490701634183	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5041	0.14893812523507022	No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC	4421	0.1306200062813421	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	4382	0.12946773750844628	No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG	4246	0.12544956947988656	No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT	4194	0.12391321111602548	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4170	0.12320412264039729	No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG	4068	0.1201904966189775	No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT	3973	0.11738368806961594	No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC	3937	0.11632005535617367	No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA	3759	0.11106098249526461	No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT	3699	0.10928826130619416	No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC	3645	0.10769281223603075	No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT	3640	0.10754508547027486	No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT	3620	0.10695417840725138	No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA	3604	0.10648145275683259	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG	3594	0.10618599922532085	No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA	3570	0.10547691074969266	No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG	3431	0.10137010666167942	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0033681702592338832	0.0	0.0	2.9545353151174413E-5	0.0
2	0.003427260965536232	0.0	0.0	2.9545353151174413E-5	0.0
3	0.003427260965536232	0.0	0.0	2.9545353151174413E-5	0.0
4	0.003427260965536232	0.0	0.0	2.9545353151174413E-5	2.9545353151174413E-5
5	0.003427260965536232	0.0	0.0	2.9545353151174413E-5	2.9545353151174413E-5
6	0.0034568063186874065	0.0	0.0	2.9545353151174413E-5	5.909070630234883E-5
7	0.0034568063186874065	0.0	0.0	2.9545353151174413E-5	5.909070630234883E-5
8	0.0034568063186874065	0.0	0.0	2.9545353151174413E-5	5.909070630234883E-5
9	0.0034568063186874065	0.0	0.0	2.9545353151174413E-5	5.909070630234883E-5
10-11	0.0035011243484141677	0.0	0.0	2.9545353151174413E-5	5.909070630234883E-5
12-13	0.0035306697015653424	0.0	0.0	4.431802972676162E-5	8.863605945352324E-5
14-15	0.003589760407867691	0.0	0.0	5.909070630234883E-5	1.1818141260469765E-4
16-17	0.0036636237907456274	0.0	0.0	5.909070630234883E-5	1.1818141260469765E-4
18-19	0.0037670325267747376	0.0	0.0	5.909070630234883E-5	1.4772676575587206E-4
20-21	0.003914759292530609	0.0	0.0	5.909070630234883E-5	1.4772676575587206E-4
22-23	0.004032940705135307	0.0	0.0	5.909070630234883E-5	1.6249944233145925E-4
24-25	0.004165894794315592	0.0	0.0	5.909070630234883E-5	1.7727211890704647E-4
26-27	0.004328394236647052	0.0	0.0	5.909070630234883E-5	1.920447954826337E-4
28-29	0.004490893678978511	0.0	0.0	5.909070630234883E-5	2.2159014863380809E-4
30-31	0.0047715745339146675	0.0	0.0	7.386338287793603E-5	2.806808549361569E-4
32-33	0.005229527507757872	0.0	0.0	8.863605945352324E-5	3.6931691438968014E-4
34-35	0.005894297953659295	0.0	0.0	8.863605945352324E-5	3.988622675408546E-4
36-37	0.006780658548194528	0.0	0.0	8.863605945352324E-5	4.136349441164418E-4
38-39	0.008021563380543852	0.0	0.0	1.1818141260469765E-4	4.4318029726761617E-4
40-41	0.010119283454277237	0.0	0.0	1.1818141260469765E-4	4.4318029726761617E-4
42-43	0.012674956501853823	0.0	0.0	1.1818141260469765E-4	4.727256504187906E-4
44-45	0.01523062954943041	0.0	0.0	1.1818141260469765E-4	4.727256504187906E-4
46-47	0.017845393303309344	0.0	0.0	1.1818141260469765E-4	5.170436801455523E-4
48-49	0.02119879088596764	0.0	0.0	1.1818141260469765E-4	5.318163567211394E-4
50-51	0.02558627582891704	0.0	0.0	1.1818141260469765E-4	5.318163567211394E-4
52-53	0.030845348689826087	0.0	0.0	1.1818141260469765E-4	5.318163567211394E-4
54-55	0.03840895909652674	0.0	0.0	1.1818141260469765E-4	5.465890332967266E-4
56-57	0.04719870165900113	0.0	0.0	1.1818141260469765E-4	6.056797395990755E-4
58-59	0.05694866819888868	0.0	0.0	1.1818141260469765E-4	6.204524161746627E-4
60-61	0.06957930667101574	0.0	0.0	1.4772676575587206E-4	6.499977693258371E-4
62-63	0.08742469997432509	0.0	0.0	1.4772676575587206E-4	7.238611522037731E-4
64-65	0.11018939457730498	0.0	0.0	1.4772676575587206E-4	7.681791819305348E-4
66-67	0.1371938473574784	0.0	0.0	1.4772676575587206E-4	7.977245350817092E-4
68-69	0.1717914558975036	0.0	0.0	1.4772676575587206E-4	8.124972116572964E-4
70-71	0.21780834343045777	0.0	0.0	1.4772676575587206E-4	8.420425648084707E-4
72-73	0.27292519973397367	0.0	0.0	1.4772676575587206E-4	8.56815241384058E-4
74-75	0.34107155677715745	0.0	0.0	1.4772676575587206E-4	8.863605945352323E-4
76-77	0.4276098961569473	0.0	0.0	1.4772676575587206E-4	8.863605945352323E-4
78-79	0.5330868069066399	0.0	0.0	1.4772676575587206E-4	9.454513008375812E-4
80-81	0.6676363451570881	0.0	0.0	1.4772676575587206E-4	9.454513008375812E-4
82-83	0.8332823675991476	0.0	0.0	1.6249944233145925E-4	9.602239774131685E-4
84-85	1.0349589482090642	0.0	0.0	1.7727211890704647E-4	9.89769330564343E-4
86-87	1.2704206401473486	0.0	0.0	2.068174720582209E-4	0.0010340873602911046
88	1.481699460531397	0.0	0.0	2.068174720582209E-4	0.001063632713442279
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	3725	0.0	89.45136	1
GTGGAAT	111410	0.0	56.015453	6
AGTGGAA	111380	0.0	55.498882	5
TGGAATC	112880	0.0	55.302635	7
GAATCAC	113690	0.0	54.941696	9
GGAATCA	113675	0.0	54.920006	8
ACGAGTG	111170	0.0	54.797897	2
AACGAGT	105345	0.0	54.61209	1
CGAGTGG	113665	0.0	53.982933	3
GAGTGGA	116490	0.0	53.08934	4
ACGTGTG	1230	0.0	50.43689	2
AACGTGT	1180	0.0	49.526024	1
ATCGAGT	720	0.0	45.16587	1
CGTGTGG	1440	0.0	43.733616	3
AGTACTT	36285	0.0	39.338085	20-21
GAGTACT	35855	0.0	38.86606	20-21
GTACTTT	36085	0.0	37.920113	22-23
TCGAGTG	955	0.0	37.40156	2
TACTTTT	37705	0.0	37.275574	22-23
CATGGGG	18440	0.0	35.808167	24-25
>>END_MODULE