##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132645_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2574454
Sequences flagged as poor quality	0
Sequence length	100
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.666800805141595	33.0	30.0	34.0	16.0	34.0
2	31.221437244557485	34.0	31.0	34.0	25.0	34.0
3	31.98716232645835	34.0	31.0	34.0	28.0	34.0
4	36.018036057354294	37.0	35.0	37.0	35.0	37.0
5	35.73627728442614	37.0	35.0	37.0	35.0	37.0
6	36.18045806994415	37.0	37.0	37.0	35.0	37.0
7	36.21093637718911	37.0	36.0	37.0	35.0	37.0
8	36.37896734608581	37.0	37.0	37.0	35.0	37.0
9	38.30518859532934	39.0	39.0	39.0	37.0	39.0
10-11	38.34244717520686	39.0	39.0	39.0	37.0	39.0
12-13	38.31333304071465	39.0	39.0	39.0	37.0	39.0
14-15	39.87767852911724	41.0	40.0	41.0	38.0	41.0
16-17	39.6864292389765	41.0	39.5	41.0	37.5	41.0
18-19	39.30590369841528	41.0	39.0	41.0	35.5	41.0
20-21	39.124036203404685	41.0	39.0	41.0	35.0	41.0
22-23	38.3727271879785	40.5	39.0	41.0	35.0	41.0
24-25	38.062550350482084	40.5	38.0	41.0	34.0	41.0
26-27	38.14180424276371	40.0	38.0	41.0	34.5	41.0
28-29	38.11486688051136	41.0	38.0	41.0	35.0	41.0
30-31	37.90916151541259	40.5	38.0	41.0	34.5	41.0
32-33	37.77202486430132	40.0	38.0	41.0	34.0	41.0
34-35	37.63606613285769	40.0	38.0	41.0	34.0	41.0
36-37	37.34336931248335	40.0	37.0	41.0	33.5	41.0
38-39	36.89463882438761	40.0	35.5	41.0	33.0	41.0
40-41	36.75638989859598	40.0	35.0	41.0	33.0	41.0
42-43	36.62630600507914	40.0	35.0	41.0	33.0	41.0
44-45	36.41751551979566	40.0	35.0	41.0	33.0	41.0
46-47	36.251801158614605	40.0	35.0	41.0	32.5	41.0
48-49	36.041073757775436	39.0	35.0	41.0	31.0	41.0
50-51	35.71191095276902	39.0	35.0	41.0	30.0	41.0
52-53	35.06578016154105	39.0	35.0	41.0	26.0	41.0
54-55	33.87071588771833	38.0	34.5	41.0	13.5	41.0
56-57	33.417815000772976	38.0	35.0	41.0	2.0	41.0
58-59	33.148952748815866	38.0	35.0	41.0	2.0	41.0
60-61	32.86486338462447	38.0	34.5	40.5	2.0	41.0
62-63	32.658090608727136	38.0	34.0	40.0	2.0	41.0
64-65	32.31419380575454	37.0	34.0	40.0	2.0	41.0
66-67	31.809350642893598	36.0	33.0	39.5	2.0	41.0
68-69	31.547955216912015	36.0	33.0	39.0	2.0	41.0
70-71	31.264147271615652	35.0	33.0	39.0	2.0	41.0
72-73	30.96538741807001	35.0	33.0	38.5	2.0	40.5
74-75	30.77539917201861	35.0	33.0	38.0	2.0	40.0
76-77	30.46429844930226	35.0	33.0	37.0	2.0	40.0
78-79	30.080523870304148	35.0	32.0	37.0	2.0	39.0
80-81	29.72833482361697	35.0	32.0	36.0	2.0	39.0
82-83	29.259034342815994	35.0	31.0	36.0	2.0	38.0
84-85	28.78481573180177	35.0	29.5	35.5	2.0	37.0
86-87	28.697441088479344	35.0	30.5	35.0	2.0	37.0
88-89	28.468656266532633	35.0	30.5	35.0	2.0	36.5
90-91	28.20359248990271	35.0	30.0	35.0	2.0	36.0
92-93	28.065598375422518	35.0	30.0	35.0	2.0	36.0
94-95	27.776207498755078	35.0	29.5	35.0	2.0	35.5
96-97	27.38514632617246	34.5	28.0	35.0	2.0	35.0
98-99	27.244352783153243	34.5	29.0	35.0	2.0	35.0
100	27.227513872844494	35.0	29.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.2726078542451766
1101	2	2.006269840850429
1101	3	1.2498946654274157
1101	4	0.5732911866674328
1101	5	0.8296105232133328
1101	6	0.37059841864657983
1101	7	0.22538715982123136
1101	8	0.17304107113567113
1101	9	0.2106872058859679
1101	10-11	0.17572416639037414
1101	12-13	0.20802278603072466
1101	14-15	0.2414860577111
1101	16-17	0.1680416659226367
1101	18-19	0.10728695722507098
1101	20-21	0.21035485535062293
1101	22-23	0.04127257066123491
1101	24-25	-0.004663964343450289
1101	26-27	0.06639745122910767
1101	28-29	0.0713744283788813
1101	30-31	0.17843545517910542
1101	32-33	0.13352050589713116
1101	34-35	0.12109808627872098
1101	36-37	0.15585067705200828
1101	38-39	0.20372032242055838
1101	40-41	0.2633826270469939
1101	42-43	0.27347637665289426
1101	44-45	0.31231801893658684
1101	46-47	0.2632214669092505
1101	48-49	0.35060165643215413
1101	50-51	0.2836893126365183
1101	52-53	0.33645352296070286
1101	54-55	0.380088341049138
1101	56-57	0.32881760132780613
1101	58-59	0.3311902886795295
1101	60-61	0.22641563781694884
1101	62-63	0.2569657686636546
1101	64-65	0.22384913140778906
1101	66-67	0.24493803075938914
1101	68-69	0.32125892321295524
1101	70-71	0.3607440216470046
1101	72-73	0.3832836899707921
1101	74-75	0.4225226794059367
1101	76-77	0.35237291418548367
1101	78-79	0.24480164575475882
1101	80-81	0.3021417073014554
1101	82-83	0.35408087999886106
1101	84-85	0.4113273537594715
1101	86-87	0.43793729542958815
1101	88-89	0.46152244350575344
1101	90-91	0.4533245218678559
1101	92-93	0.4794641212422057
1101	94-95	0.46534815005174224
1101	96-97	0.4325952869627443
1101	98-99	0.6044183549425703
1101	100	0.5118092969048718
1102	1	3.224194104317977
1102	2	1.9519947064183398
1102	3	1.1988456583806517
1102	4	0.5258322927959824
1102	5	0.8126718378329443
1102	6	0.35294632111779833
1102	7	0.21335742946190095
1102	8	0.15165933254541386
1102	9	0.1677817742473522
1102	10-11	0.12152182911628984
1102	12-13	0.09136412705143471
1102	14-15	0.1300785003954701
1102	16-17	0.19846420489338357
1102	18-19	0.09429994423805965
1102	20-21	0.06569173633211989
1102	22-23	-0.10264255755882346
1102	24-25	-0.1175741763287732
1102	26-27	-0.041965971596198415
1102	28-29	-0.051180800398320514
1102	30-31	0.054030571945752115
1102	32-33	0.07950501466189763
1102	34-35	0.06966082772262183
1102	36-37	0.05178531976355316
1102	38-39	0.04060312384932274
1102	40-41	-0.021958573232545575
1102	42-43	-0.07189154018362842
1102	44-45	-0.03582104304776834
1102	46-47	-0.030284541282853183
1102	48-49	-0.012491155091147732
1102	50-51	0.06099734653087552
1102	52-53	0.08579167546589161
1102	54-55	-0.1674633691987637
1102	56-57	-0.1732110128084301
1102	58-59	-0.25676687747270677
1102	60-61	-0.1676863364419745
1102	62-63	-0.13713256573736743
1102	64-65	-0.1214132295496384
1102	66-67	-0.19014510359503944
1102	68-69	-0.11631266065907653
1102	70-71	-0.07157646056137068
1102	72-73	-0.09992625217973483
1102	74-75	-0.06719654195565994
1102	76-77	0.07245570881984875
1102	78-79	-0.035149531617939545
1102	80-81	-0.09123714852134768
1102	82-83	-0.186087985141981
1102	84-85	-0.16417118544422138
1102	86-87	-0.24008390795596668
1102	88-89	-0.20714371843395085
1102	90-91	-0.18609174198786604
1102	92-93	-0.3285436874662757
1102	94-95	-0.3779317016361823
1102	96-97	-0.3914970538056082
1102	98-99	-0.43071780000860826
1102	100	-0.39927989417310883
1103	1	3.23696187064861
1103	2	1.9470085387057523
1103	3	1.239231151949415
1103	4	0.5065417582550538
1103	5	0.8189414849856504
1103	6	0.3910910590827328
1103	7	0.27823930437973843
1103	8	0.18402725985873047
1103	9	0.17864306044827316
1103	10-11	0.12540758330172252
1103	12-13	0.11847726985783424
1103	14-15	0.1787658889703181
1103	16-17	0.14727039892134286
1103	18-19	0.059055071236791434
1103	20-21	0.059918385193824975
1103	22-23	-0.10801676734832455
1103	24-25	-0.13130347114157814
1103	26-27	-0.12367290123200547
1103	28-29	-0.14421504150795528
1103	30-31	-0.03078696467320441
1103	32-33	-0.004343425789492983
1103	34-35	0.022239260655346982
1103	36-37	0.016750395976636412
1103	38-39	0.08386296847105257
1103	40-41	0.07211056687082618
1103	42-43	0.03591467863750353
1103	44-45	0.0594704584271355
1103	46-47	-5.14852615985717E-4
1103	48-49	0.07046228043621028
1103	50-51	0.006418550925843647
1103	52-53	-0.12238619598782208
1103	54-55	-0.12839991422894315
1103	56-57	-0.09879832285232482
1103	58-59	-0.0700664574877905
1103	60-61	-0.09534899410930109
1103	62-63	0.031083242162779356
1103	64-65	0.006629412411491842
1103	66-67	0.0998209239842005
1103	68-69	0.22517971860967378
1103	70-71	0.09142023906292351
1103	72-73	0.05666422158908446
1103	74-75	0.07193535371925464
1103	76-77	0.14993309513447528
1103	78-79	0.22225461968271887
1103	80-81	0.21444638500248914
1103	82-83	0.2806397851786251
1103	84-85	0.19845665798743184
1103	86-87	0.17130764956305455
1103	88-89	0.20060374631937705
1103	90-91	0.2918914107588719
1103	92-93	0.19693461907889542
1103	94-95	0.22648253645213856
1103	96-97	0.32162568742048947
1103	98-99	0.37348643120681047
1103	100	0.3231694066592503
1104	1	2.574243511432602
1104	2	1.7593688461412569
1104	3	1.1676884885183831
1104	4	0.5339900927779837
1104	5	0.7924727159088647
1104	6	0.3449209894866101
1104	7	0.21472149545262198
1104	8	0.17767069515885936
1104	9	0.1639238253279629
1104	10-11	0.15237570271590073
1104	12-13	0.13827147694791364
1104	14-15	0.06066789760821223
1104	16-17	0.10578878406342795
1104	18-19	0.1252875648405194
1104	20-21	0.0965185025086015
1104	22-23	0.012849897371246755
1104	24-25	0.07900092862298891
1104	26-27	0.11042633521482514
1104	28-29	0.1501552375397921
1104	30-31	0.13538429619881498
1104	32-33	0.06183123050654871
1104	34-35	0.07912592154499976
1104	36-37	0.12084327031961806
1104	38-39	0.07487534579876609
1104	40-41	0.1533929537848593
1104	42-43	0.1417466647116612
1104	44-45	0.18394983273681476
1104	46-47	0.18843042365138984
1104	48-49	0.16245018035349545
1104	50-51	0.19415489078247106
1104	52-53	-7.830407756515001E-4
1104	54-55	-0.1023623352295786
1104	56-57	-0.042022336960023665
1104	58-59	-0.0615658119975393
1104	60-61	-0.10485107387845005
1104	62-63	-0.20412216876193412
1104	64-65	-0.02211720421392016
1104	66-67	-0.09558418247457467
1104	68-69	-0.11996423514004562
1104	70-71	-0.1772585856196791
1104	72-73	-0.16136113503474903
1104	74-75	-0.0770296318336463
1104	76-77	-0.10598325140077591
1104	78-79	-0.032790930074561686
1104	80-81	0.005316679660552381
1104	82-83	0.024392204957777608
1104	84-85	-0.08217506640887606
1104	86-87	-0.0760694213398736
1104	88-89	0.027976863567538146
1104	90-91	0.04194536347126032
1104	92-93	0.0739376135263683
1104	94-95	0.0686577242183688
1104	96-97	0.10107858101955713
1104	98-99	0.162656532023572
1104	100	0.11360584957274966
1105	1	3.233154778865238
1105	2	1.9499902860213538
1105	3	1.2068532173636264
1105	4	0.5303975876472222
1105	5	0.8113479457086754
1105	6	0.3520293577142013
1105	7	0.1921446430500211
1105	8	0.14653310076899828
1105	9	0.12459801830971884
1105	10-11	0.10352484970161413
1105	12-13	0.0975336378808862
1105	14-15	0.1294957998695594
1105	16-17	0.01738663623898873
1105	18-19	0.031341607144895534
1105	20-21	0.028048371323713184
1105	22-23	-0.09570380105432719
1105	24-25	-0.018364043196271496
1105	26-27	0.0014485797352392638
1105	28-29	-0.06547117108232925
1105	30-31	-0.014071639178240503
1105	32-33	0.00586154495683644
1105	34-35	0.0011056300252576534
1105	36-37	-0.006541274807027264
1105	38-39	0.029698403013412644
1105	40-41	-0.05963787348867555
1105	42-43	-0.04439652050528764
1105	44-45	-0.022904671382733
1105	46-47	-0.021199647911480213
1105	48-49	8.003313931439493E-4
1105	50-51	-0.006239815692246964
1105	52-53	-0.04171706757264815
1105	54-55	-0.07069770056310887
1105	56-57	-0.2924590594348828
1105	58-59	-0.26439614024627645
1105	60-61	-0.3009522387652055
1105	62-63	-0.2612401858030253
1105	64-65	-0.24022340120588836
1105	66-67	-0.2456036749542001
1105	68-69	-0.2660607668642889
1105	70-71	-0.22612260922830885
1105	72-73	-0.17445458216971588
1105	74-75	-0.20249274962292674
1105	76-77	-0.26805874510321104
1105	78-79	-0.326103205371588
1105	80-81	-0.27932274122011336
1105	82-83	-0.267832864839157
1105	84-85	-0.17732095545863658
1105	86-87	-0.2314598100246812
1105	88-89	-0.30205210596660237
1105	90-91	-0.22872041765035078
1105	92-93	-0.24372045128681208
1105	94-95	-0.2803273028726423
1105	96-97	-0.2553266145886681
1105	98-99	-0.25425765762115304
1105	100	-0.3089564682671053
1106	1	1.8266152825886692
1106	2	1.3861979725390867
1106	3	1.006877858403147
1106	4	0.423420414898672
1106	5	0.6344023519178279
1106	6	0.22067704989221681
1106	7	0.1166779183406419
1106	8	0.10693199109476836
1106	9	0.08565528860144411
1106	10-11	0.06829124857988234
1106	12-13	0.03284032065141673
1106	14-15	0.09138756273608806
1106	16-17	0.0953467410356339
1106	18-19	0.04425396063625442
1106	20-21	0.002151017451595294
1106	22-23	-0.1310046496345123
1106	24-25	-0.16248081619900034
1106	26-27	-0.11579354238978823
1106	28-29	-0.05772407453869022
1106	30-31	0.031242278829786585
1106	32-33	0.029600879502915234
1106	34-35	0.011189593280519716
1106	36-37	0.08782696442170845
1106	38-39	0.027938819619485855
1106	40-41	-0.027072678164607566
1106	42-43	-0.11952578077904263
1106	44-45	-0.039669729238688944
1106	46-47	-0.01854505624239522
1106	48-49	7.184633975967358E-4
1106	50-51	0.02426062320434852
1106	52-53	-0.023193969017320626
1106	54-55	-0.07706902910781821
1106	56-57	-0.09818227394473666
1106	58-59	-0.06649444326823328
1106	60-61	-0.11460381642984885
1106	62-63	-0.06970322585656419
1106	64-65	-0.06682628539959268
1106	66-67	-0.0840876213239703
1106	68-69	-0.10283681010798418
1106	70-71	-0.07092813224313943
1106	72-73	-0.0578014888777858
1106	74-75	-0.03943375600112731
1106	76-77	-0.06825872740429517
1106	78-79	-0.009007211482902733
1106	80-81	0.030701786220983962
1106	82-83	-0.01808046731650137
1106	84-85	0.04817043132700505
1106	86-87	0.037580336637891065
1106	88-89	0.10252094559964675
1106	90-91	0.15337525858350176
1106	92-93	0.1645083656585946
1106	94-95	0.2290846065330392
1106	96-97	0.23304360714281813
1106	98-99	0.1933400934667624
1106	100	0.17405404687928083
1107	1	2.8155890445032377
1107	2	1.7871696121577614
1107	3	1.1639634259177747
1107	4	0.4764851345778567
1107	5	0.8129482191577111
1107	6	0.35101747039862374
1107	7	0.16152283932085965
1107	8	0.15616580277769287
1107	9	0.12001435349606027
1107	10-11	0.15430647278802212
1107	12-13	0.0990286175788242
1107	14-15	0.0977375214819105
1107	16-17	0.17137447115042193
1107	18-19	0.22228631718411407
1107	20-21	0.20574433606213205
1107	22-23	0.020899894612661285
1107	24-25	0.050379845455893246
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2216	52-53	-0.33817430939452464
2216	54-55	-0.23096946297635412
2216	56-57	-0.2970822261228605
2216	58-59	-0.21775321748330612
2216	60-61	-0.35463981157764124
2216	62-63	-0.4614966388197388
2216	64-65	-0.44867485987080613
2216	66-67	-0.47808092741840724
2216	68-69	-0.462218273330123
2216	70-71	-0.45740171579125644
2216	72-73	-0.5282279830655341
2216	74-75	-0.5028591910485751
2216	76-77	-0.3911825043084427
2216	78-79	-0.3357876361631611
2216	80-81	-0.42700420441657627
2216	82-83	-0.49246906042465355
2216	84-85	-0.3454458254495698
2216	86-87	-0.34274589361822905
2216	88-89	-0.588522795072123
2216	90-91	-0.6521161278372247
2216	92-93	-0.6441288786440786
2216	94-95	-0.666052027194489
2216	96-97	-0.8242626527784829
2216	98-99	-0.9395077788647406
2216	100	-0.9245863132226368
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	288.0
3	97.0
4	208.0
5	626.0
6	2287.0
7	4952.0
8	19772.0
9	27877.0
10	3613.0
11	6589.0
12	5914.0
13	7912.0
14	8325.0
15	9539.0
16	12184.0
17	15670.0
18	20006.0
19	28771.0
20	57414.0
21	73591.0
22	33588.0
23	18474.0
24	16163.0
25	16818.0
26	17782.0
27	20068.0
28	22587.0
29	26170.0
30	29895.0
31	35799.0
32	45327.0
33	62885.0
34	95405.0
35	273210.0
36	281091.0
37	468818.0
38	561522.0
39	239251.0
40	3966.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	12.092725019682272	48.767622985464534	23.455306928510016	15.68434506634318
2	14.399898384642096	47.61848715863105	22.398689565858483	15.58292489086837
3	15.164493514726264	24.625511954407315	18.638752851096623	41.5712416797698
4	38.27733428010623	26.086571453321	20.566362344956552	15.069731921616217
5	14.344650568218235	46.95902155449841	21.540592537981883	17.15573533930147
6	41.04781052603775	22.685664610826215	22.61155180865535	13.65497305448068
7	16.49184642646557	23.247142889327215	45.24120454278849	15.019806141418723
8	40.15169818532396	24.16454129691189	21.143395842380556	14.540364675383596
9	39.69175599952456	25.31635834239027	22.13257646087287	12.859309197212301
10-11	13.334944030850812	49.777739279862836	22.001247643189583	14.886069046096765
12-13	13.070829775944725	24.2133477622828	34.55078241833026	28.165040043442218
14-15	13.90537244639731	36.56492199696168	21.539446662608842	27.990258894032166
16-17	27.140983513588417	23.25009948713387	34.542670900833905	15.066246098443806
18-19	14.51092435734419	24.16481789241872	34.563160686335955	26.761097063901136
20-21	27.29239963137722	36.73815449722708	22.592711640070345	13.376734231325363
22-23	26.23970385159951	24.24252782703442	34.611314935411755	14.906453385954316
24-25	13.604671126382526	36.69226562214745	21.943623774206102	27.75943947726392
26-27	14.418960292162922	31.293276166519192	39.601426166480344	14.686337374837539
28-29	29.723273362041034	23.64225579482096	32.225706887751734	14.40876395538627
30-31	22.915460909381174	28.14503580176612	33.038267531678564	15.901235757174142
32-33	15.816557607943277	29.20190067486154	34.21049667230411	20.771045044891075
34-35	18.93523233745157	29.565739927175322	33.74851713135711	17.750510604016
36-37	19.617577163934566	26.933361404010327	36.76397014667965	16.68509128537546
38-39	22.45876254321555	27.254719892544017	34.25747912320673	16.029038441033705
40-41	15.438985400202412	33.94382535944036	34.064685961506	16.552503278851223
42-43	15.215472362333138	27.497229018176128	37.317118598410495	19.970180021080246
44-45	15.388658114326246	30.933895064600907	33.232896549206	20.444550271866852
46-47	19.12941015154702	27.46400105188925	36.1536239973695	17.252964799194224
48-49	16.18292156636535	27.80946809597447	36.049507592663595	19.958102744996587
50-51	19.392600234692456	31.237713313960786	32.88370682010368	16.485979631243076
52-53	19.100348910492272	28.311294581254	35.12570197236877	17.46265453588495
54-55	16.437756487320566	32.112746630827225	30.12383251535768	21.325664366494525
56-57	17.127384179383164	32.94516545219755	31.232660094245574	18.69479027417371
58-59	23.976933927717216	30.05517142529192	27.621002004132567	18.346892642858297
60-61	20.312705751745746	33.500239473182305	27.443762646145647	18.7432921289263
62-63	17.263363927582173	33.11186199454718	27.247578025433793	22.37719605243685
64-65	19.78253033295163	33.105179092209994	27.677216065083194	19.435074509755175
66-67	20.193035630564545	30.04311217127406	30.929729763323934	18.83412243483746
68-69	22.9872145432313	30.282233262774533	28.329193611758015	18.401358582236156
70-71	16.200226187879665	36.341260526447144	28.186280460152304	19.27223282552089
72-73	16.06433760052143	30.4463165156377	31.20756619566657	22.281779688174293
74-75	16.374041057313214	33.67635805706073	27.238031424187692	22.711569461438366
76-77	19.994631813072118	30.307940488481705	29.691706034665593	20.005721663780587
78-79	17.526235312099853	30.709157970440565	29.240120329057905	22.524486388401673
80-81	20.888885867691627	33.673580568695066	26.468297012607778	18.969236551005526
82-83	20.35405294072063	31.0395189954497	29.18508082614295	19.421347237686717
84-85	17.767008148047296	33.93742397560926	25.92849536429655	22.36707251204689
86-87	17.875099201763277	34.128397672531435	28.72640279936402	19.270100326341268
88-89	24.151028853181263	30.68549490550306	26.169735769037818	18.99374047227786
90-91	20.517990345535363	33.84475633295045	26.038794990430603	19.598458331083577
92-93	17.646512697521093	33.74375845381353	25.554717781501797	23.055011067163584
94-95	20.216915155801065	34.07291893465645	25.354728978417445	20.35543693112504
96-97	21.059123657328865	31.156165969252942	28.036517353123113	19.74819302029508
98-99	23.633969115983934	31.45140192706099	25.509815052874636	19.40481390408044
100	17.483725188657296	36.78653126562271	25.735197292393458	19.994546253326533
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	164057.0
1	91050.0
2	12068.5
3	4820.5
4	3336.5
5	3010.5
6	3099.5
7	3536.5
8	3386.0
9	3094.5
10	3200.5
11	4163.0
12	28590.0
13	30553.5
14	7616.5
15	6253.0
16	6806.0
17	7807.0
18	8929.5
19	10116.0
20	11496.0
21	13083.0
22	14808.5
23	16864.5
24	18971.5
25	21286.5
26	23727.5
27	26295.0
28	29088.0
29	32137.0
30	35252.0
31	37883.0
32	40239.5
33	42500.0
34	45188.5
35	47500.0
36	50384.0
37	53650.0
38	55624.5
39	56865.0
40	57020.5
41	56300.0
42	54700.0
43	52185.0
44	50691.5
45	51104.0
46	54327.0
47	65020.5
48	73778.5
49	112952.5
50	265526.5
51	266357.0
52	103496.0
53	48563.0
54	42241.5
55	41232.0
56	37975.5
57	32682.0
58	27280.5
59	21140.5
60	16007.0
61	12264.5
62	9193.0
63	6569.5
64	4664.0
65	3246.0
66	2491.5
67	2198.5
68	1757.0
69	1248.0
70	989.0
71	785.0
72	596.5
73	442.0
74	320.0
75	256.0
76	199.0
77	134.0
78	83.5
79	47.5
80	29.5
81	18.0
82	10.0
83	7.0
84	5.0
85	3.5
86	2.0
87	2.0
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.28833298244987093
2	0.00155372750882323
3	2.3305912632348453E-4
4	0.003690102833455172
5	1.1652956316174226E-4
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.1652956316174226E-4
16-17	1.9421593860290376E-5
18-19	1.9421593860290376E-5
20-21	2.524807201837749E-4
22-23	0.0026801799527200717
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.002019845761470199
36-37	0.0
38-39	1.7479434474261337E-4
40-41	1.9421593860290376E-5
42-43	2.1363753246319414E-4
44-45	0.001242982007058584
46-47	0.001495462727242359
48-49	0.002369434450955426
50-51	8.157069421321957E-4
52-53	4.4669665878667864E-4
54-55	3.4958868948522674E-4
56-57	3.6901028334551715E-4
58-59	9.128149114336477E-4
60-61	0.0020586889491907797
62-63	1.9421593860290376E-4
64-65	0.0010099228807350996
66-67	2.7190231404406525E-4
68-69	2.1363753246319414E-4
70-71	0.001767365041286424
72-73	1.1652956316174226E-4
74-75	1.55372750882323E-4
76-77	0.001087609256176261
78-79	5.243830342278402E-4
80-81	0.0020004241676099084
82-83	3.3016709562493643E-4
84-85	0.0013012467886394552
86-87	0.006020694096690017
88-89	0.002272326481653974
90-91	0.003923161959778656
92-93	0.006681028287939889
94-95	0.005030192809815207
96-97	0.002388856044815716
98-99	6.020694096690016E-4
100	0.002990925454484718
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2574454.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.77543597909256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.00076245265905	35.92719345994206
2	8.725183875142871	7.289967207636836
3	2.285352774481944	2.864148255600361
4	0.888013316046931	1.4838857373240102
5	0.47838677156502357	0.9992407974379711
6	0.3095745666184203	0.7759567493113888
7	0.20519470226426256	0.6000468703382763
8	0.13883880729462839	0.46400413644402544
9	0.10842028427915806	0.40763741802650855
>10	0.7162408662100375	5.931450038898319
>50	0.08603734950234182	2.4263494338459335
>100	0.04514869113794798	3.8324475858038864
>500	0.004747265816476536	1.2821135332270173
>1k	0.003909513025333618	4.396967745672819
>5k	0.003537178451492321	10.561853045397172
>10k+	6.515855042222697E-4	20.756737985093473
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	198096	7.6946801146961645	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	156754	6.088825047951915	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	127258	4.943106382945666	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16655	0.6469332914862724	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	14215	0.5521559134480554	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10641	0.4133303605346998	No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC	10164	0.39480215999198276	No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG	9919	0.38528557900044047	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	9622	0.373749152247428	No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT	9360	0.3635722370646358	No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT	9031	0.35079282830456476	No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG	8857	0.3440341136411837	No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC	8673	0.33688696710059685	No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA	8605	0.3342456303355974	No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT	8346	0.32418524471596694	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8324	0.3233306945861142	No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT	8227	0.3195629053772178	No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA	8225	0.3194852190017767	No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA	8058	0.31299840665243966	No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT	8039	0.3122603860857487	No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG	7995	0.3105512858260431	No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC	7885	0.3062785351767792	No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA	7756	0.30126776396082433	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG	7682	0.29839336806950134	No Hit
TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA	7252	0.2816907973496516	No Hit
ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG	7104	0.27594200556700565	No Hit
CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC	7019	0.27264033461075626	No Hit
CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT	6901	0.2680568384597278	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6672	0.2591617484717148	No Hit
GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC	6624	0.2572972754611269	No Hit
GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT	6592	0.25605429345406827	No Hit
CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG	6260	0.2431583551308355	No Hit
GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT	6075	0.23597236540252808	No Hit
GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT	5977	0.23216573300591115	No Hit
TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG	5961	0.23154424200238188	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5918	0.2298739849303969	No Hit
TACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	5903	0.2292913371145882	No Hit
ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG	5852	0.22731033454083857	No Hit
CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT	5816	0.22591197978289765	No Hit
ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC	5490	0.2132491005859883	No Hit
TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA	5249	0.20388789234532834	No Hit
ACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATC	5190	0.2015961442698141	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5102	0.19817794375040299	No Hit
ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC	5030	0.19538123423452117	No Hit
CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG	5019	0.1949539591695948	No Hit
TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG	4716	0.18318447329025883	No Hit
TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC	4690	0.18217455040952374	No Hit
ACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAAC	4651	0.18065966608842107	No Hit
GCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCC	4508	0.17510509024437804	No Hit
GAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATG	4348	0.1688901802090851	No Hit
GGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGC	4326	0.16803563007923233	No Hit
AGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGA	4077	0.15836367633680773	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGAAACGAGTGGAATCACTGCTG	4009	0.15572233957180823	No Hit
TGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACA	4006	0.15560581000864648	No Hit
TACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAAT	3987	0.15486778944195545	No Hit
GGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACAT	3958	0.15374133699805861	No Hit
CAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCC	3952	0.15350827787173513	No Hit
TCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGA	3846	0.14939089997335356	No Hit
AGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCA	3638	0.1413115169274728	No Hit
GGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTG	3476	0.1350189205167387	No Hit
GAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGG	3307	0.12845442179196054	No Hit
TGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACT	3293	0.12791061716387242	No Hit
GAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCT	3244	0.12600730096556395	No Hit
CACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAA	3203	0.12441473026902015	No Hit
TCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2678	0.10402205671571525	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0034958868948522675	0.0	0.0	0.0	0.0
2	0.0035347300825728485	0.0	0.0	0.0	0.0
3	0.003573573270293429	0.0	0.0	0.0	0.0
4	0.003573573270293429	0.0	0.0	0.0	0.0
5	0.003573573270293429	0.0	0.0	0.0	0.0
6	0.003573573270293429	0.0	0.0	0.0	0.0
7	0.003573573270293429	0.0	0.0	0.0	0.0
8	0.003573573270293429	0.0	0.0	0.0	0.0
9	0.003573573270293429	0.0	0.0	0.0	0.0
10-11	0.0036512596457345904	0.0	0.0	0.0	0.0
12-13	0.0037483676150360428	0.0	0.0	0.0	0.0
14-15	0.003903740365918366	0.0	0.0	0.0	0.0
16-17	0.00407853471066098	0.0	0.0	0.0	0.0
18-19	0.004447544994006496	0.0	0.0	0.0	0.0
20-21	0.0047194473080505615	0.0	0.0	0.0	0.0
22-23	0.0051078791852563685	0.0	0.0	0.0	0.0
24-25	0.005612840625623918	0.0	0.0	0.0	0.0
26-27	0.006059537284410598	0.0	0.0	0.0	0.0
28-29	0.0066227635063590175	0.0	0.0	0.0	0.0
30-31	0.0073607840730500525	0.0	0.0	0.0	0.0
32-33	0.008681452455549798	0.0	0.0	0.0	0.0
34-35	0.010565347059997964	0.0	0.0	0.0	0.0
36-37	0.012585192821468163	0.0	0.0	0.0	0.0
38-39	0.01542074552507056	0.0	0.0	0.0	1.9421593860290376E-5
40-41	0.01868357329359934	0.0	0.0	3.884318772058075E-5	3.884318772058075E-5
42-43	0.02181044990510609	0.0	0.0	3.884318772058075E-5	5.826478158087113E-5
44-45	0.0258307198341862	0.0	0.0	3.884318772058075E-5	7.76863754411615E-5
46-47	0.030258843234332408	0.0	0.0	3.884318772058075E-5	7.76863754411615E-5
48-49	0.03474523141605948	0.0	0.0	3.884318772058075E-5	7.76863754411615E-5
50-51	0.0401638561030805	0.0	0.0	3.884318772058075E-5	7.76863754411615E-5
52-53	0.04719447308050562	0.0	0.0	3.884318772058075E-5	9.710796930145188E-5
54-55	0.05624493581940093	0.0	0.0	7.76863754411615E-5	1.1652956316174225E-4
56-57	0.06630532143903134	0.0	0.0	1.55372750882323E-4	1.1652956316174225E-4
58-59	0.07774464022274238	0.0	0.0	1.55372750882323E-4	1.1652956316174225E-4
60-61	0.09188356055303376	0.0	0.0	1.9421593860290376E-4	1.1652956316174225E-4
62-63	0.11031465312644934	0.0	0.0	0.0013983547579409072	1.1652956316174225E-4
64-65	0.13321271228773168	0.0	0.0	0.0013983547579409072	1.1652956316174225E-4
66-67	0.16096616991408663	0.0	0.0	0.0013983547579409072	1.1652956316174225E-4
68-69	0.19322543731602895	0.0	0.0	0.0013983547579409072	1.1652956316174225E-4
70-71	0.23418557876738136	0.0	0.0	0.0013983547579409072	1.1652956316174225E-4
72-73	0.284662301210276	0.0	0.0	0.0013983547579409072	1.1652956316174225E-4
74-75	0.3463841264982789	0.0	0.0	0.0013983547579409072	1.1652956316174225E-4
76-77	0.41820518059363265	0.0	0.0	0.0013983547579409072	1.1652956316174225E-4
78-79	0.5050585483368513	0.0	0.0	0.0013983547579409072	1.1652956316174225E-4
80-81	0.6226368775670492	0.0	0.0	0.0013983547579409072	1.1652956316174225E-4
82-83	0.7694447055569842	0.0	0.0	0.0013983547579409072	1.1652956316174225E-4
84-85	0.9497159397681993	0.0	0.0	0.0013983547579409072	1.1652956316174225E-4
86-87	1.1648877781463565	0.0	0.0	0.0013983547579409072	1.1652956316174225E-4
88	1.346188356832167	0.0	0.0	0.0013983547579409072	1.1652956316174225E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	3370	0.0	87.68857	1
AACGTGT	880	0.0	63.198196	1
CGTGTGG	930	0.0	62.665382	3
ACGTGTG	990	0.0	58.392746	2
ATCGAGT	485	0.0	52.475597	1
GTGTGGA	1365	0.0	46.13825	4
ATCTGCG	635	0.0	42.932426	94
GTGGAAT	156925	0.0	38.860104	6
AGTGGAA	156840	0.0	38.49163	5
TGGAATC	159255	0.0	38.291553	7
GGAATCA	160490	0.0	38.00275	8
ATGAGTG	1175	0.0	37.999226	2
GAATCAC	161475	0.0	37.78549	9
AGTACTT	32745	0.0	37.03587	20-21
ACGAGTG	165040	0.0	36.231747	2
GAGTACT	32655	0.0	36.116127	20-21
CGAGTGG	167870	0.0	35.800125	3
AACGAGT	159320	0.0	35.641014	1
GAGTGGA	170245	0.0	35.51603	4
TACTTTT	33590	0.0	35.50222	22-23
>>END_MODULE