FastQCFastQC Report
Thu 26 May 2016
SRR2132644_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132644_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1964917
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1287946.554678899923No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA497242.5305903506356757No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG300321.5284106147995056No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT73910.376148203715475No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52270.26601632537150427No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT30830.15690230172572175No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT30060.1529835611376969No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29260.14891214234494385No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG21500.10941938005523898No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21410.10896134544105425No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20730.10550063946721414No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG20150.10254886084246814No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA19900.10127654246973282No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC19850.10102207879518574No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT22150.087.020881
GTGGAAT575700.057.7642636
TGGAATC581100.057.2274787
GAATCAC584050.057.0364389
GGAATCA584450.056.9798668
AGTGGAA577550.056.968945
ACGAGTG578300.056.3276182
AACGAGT545250.056.112131
CGAGTGG589300.055.6271063
GAGTGGA600850.054.753354
AACGTGT6900.048.493461
CGTGTGG8800.042.191323
ACGTGTG8250.042.1557162
ATCGAGT3750.041.4721571
GAGTACT196450.040.57443620-21
AGTACTT201650.040.34386420-21
GTACTTT199550.039.44902422-23
TACTTTT210150.038.39848322-23
CATGGGG102150.038.1402724-25
ACTTTTT217500.035.8903824-25
Produced by FastQC (version 0.11.5)