##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132644_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1964917
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.608689832700314	33.0	30.0	34.0	16.0	34.0
2	31.17372387739533	33.0	31.0	34.0	25.0	34.0
3	31.943300912964773	34.0	31.0	34.0	28.0	34.0
4	35.99080978993006	37.0	35.0	37.0	35.0	37.0
5	35.744777005848086	37.0	35.0	37.0	35.0	37.0
6	36.18725727346244	37.0	37.0	37.0	35.0	37.0
7	36.238236017093854	37.0	36.0	37.0	35.0	37.0
8	36.379593132941494	37.0	37.0	37.0	35.0	37.0
9	38.30394718962684	39.0	39.0	39.0	37.0	39.0
10-11	38.34119939926215	39.0	39.0	39.0	37.0	39.0
12-13	38.31011208107009	39.0	39.0	39.0	37.0	39.0
14-15	39.85921619081111	41.0	40.0	41.0	38.0	41.0
16-17	39.7484888674687	41.0	40.0	41.0	37.5	41.0
18-19	39.6048609177894	41.0	40.0	41.0	36.5	41.0
20-21	39.52706831891627	41.0	39.5	41.0	36.0	41.0
22-23	39.16436826593693	41.0	39.0	41.0	36.0	41.0
24-25	39.075420234035334	41.0	39.0	41.0	35.0	41.0
26-27	39.04662843265136	41.0	39.0	41.0	35.0	41.0
28-29	38.960111037769025	41.0	39.0	41.0	35.0	41.0
30-31	38.824778094952606	41.0	39.0	41.0	35.0	41.0
32-33	38.70479287420283	41.0	39.0	41.0	35.0	41.0
34-35	38.61957986011623	41.0	39.0	41.0	35.0	41.0
36-37	38.45837203301717	40.5	38.5	41.0	35.0	41.0
38-39	38.075130654373694	40.0	37.0	41.0	35.0	41.0
40-41	37.90954376189936	40.0	37.0	41.0	35.0	41.0
42-43	37.79360349572018	40.0	36.0	41.0	34.0	41.0
44-45	37.63364508526314	40.0	36.0	41.0	34.0	41.0
46-47	37.523758255437755	40.0	35.0	41.0	33.5	41.0
48-49	37.37736454007981	40.0	35.0	41.0	33.0	41.0
50-51	37.13823942690709	40.0	35.0	41.0	33.0	41.0
52-53	36.673367882714636	40.0	35.0	41.0	32.5	41.0
54-55	35.836039893797036	39.0	35.0	41.0	29.0	41.0
56-57	35.302039475458756	39.0	35.0	41.0	26.5	41.0
58-59	35.00308791669063	39.0	35.0	41.0	25.0	41.0
60-61	34.75853178531205	39.0	35.0	41.0	24.5	41.0
62-63	34.50314135406228	38.0	35.0	40.5	23.5	41.0
64-65	34.19853892047348	37.5	35.0	40.0	22.5	41.0
66-67	33.828954098315606	37.0	35.0	40.0	21.5	41.0
68-69	33.53358004434793	36.5	34.0	39.5	21.0	41.0
70-71	33.21824077047529	36.0	34.0	39.0	20.0	41.0
72-73	32.88536945835371	35.5	34.0	39.0	19.5	40.5
74-75	32.70237267019421	35.0	34.0	38.5	19.0	40.0
76-77	32.39881557338045	35.0	34.0	37.0	17.0	39.5
78-79	32.06498366088746	35.0	34.0	37.0	11.5	39.0
80-81	31.758122353259704	35.0	34.0	36.0	7.0	39.0
82-83	31.430275171928383	35.0	34.0	36.0	2.0	37.5
84-85	31.1169688083517	35.0	33.0	35.5	2.0	37.0
86-87	30.94731431403973	35.0	33.0	35.0	2.0	37.0
88-89	30.75278116073096	35.0	33.0	35.0	2.0	36.0
90-91	30.573351189897586	35.0	33.0	35.0	2.0	36.0
92-93	30.44035651378659	35.0	33.0	35.0	2.0	36.0
94-95	30.26513638998492	35.0	33.0	35.0	2.0	36.0
96-97	30.053180872270943	35.0	33.0	35.0	2.0	35.0
98-99	29.941254007166712	35.0	33.0	35.0	2.0	35.0
100	29.882959432892076	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.316641188918002
1101	2	2.067929020006222
1101	3	1.345085022231629
1101	4	0.6231485575670206
1101	5	0.8269787484309248
1101	6	0.34383117070228053
1101	7	0.1692594474753193
1101	8	0.12850458435302414
1101	9	0.19846634387162254
1101	10-11	0.15263086592092634
1101	12-13	0.17550305719441184
1101	14-15	0.2052813729423164
1101	16-17	0.21889636375254895
1101	18-19	0.15885778141728935
1101	20-21	0.18968454516100763
1101	22-23	0.17760859294381248
1101	24-25	0.22232089909476116
1101	26-27	0.25048695610264105
1101	28-29	0.2904832131064907
1101	30-31	0.30897983657320083
1101	32-33	0.2587193230543292
1101	34-35	0.17162505831883124
1101	36-37	0.3663804177871839
1101	38-39	0.34816745551609074
1101	40-41	0.425069451268115
1101	42-43	0.4336378818439641
1101	44-45	0.44958892160168773
1101	46-47	0.4313266690184321
1101	48-49	0.44026134707726783
1101	50-51	0.3997945165948309
1101	52-53	0.5370815488337257
1101	54-55	0.5169714196084954
1101	56-57	0.4350073260283196
1101	58-59	0.43208096765644655
1101	60-61	0.36761697072579125
1101	62-63	0.3867948212071539
1101	64-65	0.379341849691194
1101	66-67	0.47103897217073154
1101	68-69	0.4279383668751038
1101	70-71	0.4240137633089205
1101	72-73	0.4052453073747557
1101	74-75	0.43758415633746495
1101	76-77	0.4488899933725108
1101	78-79	0.4445467219139374
1101	80-81	0.46665143382366026
1101	82-83	0.4940961788689364
1101	84-85	0.42127459804929757
1101	86-87	0.3825810544648114
1101	88-89	0.37625255371936817
1101	90-91	0.40042249372497807
1101	92-93	0.4065406258962696
1101	94-95	0.44734553112235886
1101	96-97	0.41076938268621177
1101	98-99	0.41925890691988243
1101	100	0.43876099315620465
1102	1	3.3759829869567746
1102	2	2.1168975939475807
1102	3	1.3460332898364058
1102	4	0.6480070264811815
1102	5	0.8764868260587519
1102	6	0.432250438584461
1102	7	0.2623477795418907
1102	8	0.21907801071767352
1102	9	0.2065386226869279
1102	10-11	0.17867488703334544
1102	12-13	0.1837702017060181
1102	14-15	0.19727322005515902
1102	16-17	0.29504860903080043
1102	18-19	0.23061929765029276
1102	20-21	0.19312949451335015
1102	22-23	0.18963777117407687
1102	24-25	0.16809580278147251
1102	26-27	0.15673453816331318
1102	28-29	0.19072951638045765
1102	30-31	0.2400988246896958
1102	32-33	0.21762337300164347
1102	34-35	0.23889894133969847
1102	36-37	0.2252205905975373
1102	38-39	0.2443770596022432
1102	40-41	0.2302321793629858
1102	42-43	0.19291779307527435
1102	44-45	0.20760261009273506
1102	46-47	0.1870682720049217
1102	48-49	0.24466376930825362
1102	50-51	0.21064473559327723
1102	52-53	0.31228042628789154
1102	54-55	0.18699562699087835
1102	56-57	0.14399285508899595
1102	58-59	0.14682010387832634
1102	60-61	0.14910852364305072
1102	62-63	0.24347386938183035
1102	64-65	0.17467303429798875
1102	66-67	0.21799140453995136
1102	68-69	0.22278590141664978
1102	70-71	0.2668945413287247
1102	72-73	0.1869311040103554
1102	74-75	0.21119388009217488
1102	76-77	0.2230756687224016
1102	78-79	0.21355169400577978
1102	80-81	0.21778865833406869
1102	82-83	0.29412646919580965
1102	84-85	0.18956031482292346
1102	86-87	0.2466674248128804
1102	88-89	0.3255114669745893
1102	90-91	0.3046646944322937
1102	92-93	0.36405561245532425
1102	94-95	0.369186162952456
1102	96-97	0.3584145741123699
1102	98-99	0.31097254328917145
1102	100	0.3347486133067967
1103	1	3.2666704573017107
1103	2	2.0055412011539424
1103	3	1.2823804566093102
1103	4	0.5727368358739895
1103	5	0.8284262075079809
1103	6	0.37436997905673763
1103	7	0.2816505320113478
1103	8	0.18752813508923083
1103	9	0.15272504102586026
1103	10-11	0.14657240870850075
1103	12-13	0.13892359328709603
1103	14-15	0.13583415253027198
1103	16-17	0.22807440833968684
1103	18-19	0.09971680111305403
1103	20-21	0.09428011109908851
1103	22-23	0.09665769458612772
1103	24-25	0.031911708920489446
1103	26-27	0.14632686786534777
1103	28-29	0.18755444724521197
1103	30-31	0.1935228765662913
1103	32-33	0.12881453615123917
1103	34-35	0.19445132836288792
1103	36-37	0.29432396516440207
1103	38-39	0.2584125929620882
1103	40-41	0.23700216148607467
1103	42-43	0.2809370263330422
1103	44-45	0.32518908306888505
1103	46-47	0.2812817597283299
1103	48-49	0.32390785866405736
1103	50-51	0.33226214090075246
1103	52-53	0.2327702201522328
1103	54-55	0.35353788216583837
1103	56-57	0.4982504839297732
1103	58-59	0.6065982480342953
1103	60-61	0.4730021200657504
1103	62-63	0.5171227965274241
1103	64-65	0.5482649278608562
1103	66-67	0.586557992445961
1103	68-69	0.46921495211902453
1103	70-71	0.4636284101706494
1103	72-73	0.48320810509829926
1103	74-75	0.5117663726514508
1103	76-77	0.5743792934701801
1103	78-79	0.5754935197514115
1103	80-81	0.5897163934298497
1103	82-83	0.5172696426593788
1103	84-85	0.4872538096395793
1103	86-87	0.48790162675010507
1103	88-89	0.5089371004547445
1103	90-91	0.6356692762898639
1103	92-93	0.5679533066690539
1103	94-95	0.6060533418030616
1103	96-97	0.568085840209978
1103	98-99	0.6371303489819837
1103	100	0.676410447295936
1104	1	2.667732566548988
1104	2	1.7874975754891942
1104	3	1.2081466075640108
1104	4	0.5323354947105798
1104	5	0.8124478429002338
1104	6	0.3143616228099688
1104	7	0.2298843876810821
1104	8	0.19288315964930547
1104	9	0.1690508333906351
1104	10-11	0.1310892203319156
1104	12-13	0.12342751092838
1104	14-15	0.032805438933294795
1104	16-17	0.13484405652087617
1104	18-19	0.14605404664617083
1104	20-21	0.09621462244556511
1104	22-23	0.06578713549832571
1104	24-25	0.06737826287719173
1104	26-27	0.10820989082041876
1104	28-29	0.2101370740119819
1104	30-31	0.1512173071704055
1104	32-33	0.08730295215037387
1104	34-35	0.0961728051000108
1104	36-37	0.11329245605745797
1104	38-39	0.15257204491344112
1104	40-41	0.1443408009887861
1104	42-43	0.12615985327119716
1104	44-45	0.30143541064984447
1104	46-47	0.2571305031447082
1104	48-49	0.1576491859824074
1104	50-51	0.10039403711105876
1104	52-53	0.164103791491911
1104	54-55	0.0971515164754706
1104	56-57	0.1706906972553881
1104	58-59	0.13982509363631124
1104	60-61	0.19546808586760278
1104	62-63	0.12874653214826992
1104	64-65	0.14121924199800873
1104	66-67	0.1372587862894008
1104	68-69	0.09012318292744936
1104	70-71	-0.019462551786659787
1104	72-73	0.052642908629508156
1104	74-75	-0.04578656720013896
1104	76-77	-0.03192709404294902
1104	78-79	0.04526907929108859
1104	80-81	0.07469880793092543
1104	82-83	0.056123942554002326
1104	84-85	-0.010477440972589847
1104	86-87	0.02072763511865716
1104	88-89	0.1201393566413671
1104	90-91	0.03598264737298962
1104	92-93	0.04387633834944893
1104	94-95	0.044434302430751416
1104	96-97	0.010853238923608899
1104	98-99	0.00936186906655223
1104	100	0.03848955341412008
1105	1	3.3622164953274414
1105	2	2.068778393887005
1105	3	1.328541135266704
1105	4	0.6244718172957491
1105	5	0.888432432677881
1105	6	0.37954465153546124
1105	7	0.28256200696381484
1105	8	0.2337866751255646
1105	9	0.2393520522847794
1105	10-11	0.1494745620384208
1105	12-13	0.1637859586713546
1105	14-15	0.24137391319336388
1105	16-17	0.23628907041781844
1105	18-19	0.18089008668844286
1105	20-21	0.11915510949772568
1105	22-23	0.16438227636811575
1105	24-25	0.11639306496367396
1105	26-27	0.18866751276826932
1105	28-29	0.20870976428523846
1105	30-31	0.21030776373271465
1105	32-33	0.20278225874020706
1105	34-35	0.18539268602879133
1105	36-37	0.14622897628382248
1105	38-39	0.21767224849952527
1105	40-41	0.20223183561835611
1105	42-43	0.19248060038717796
1105	44-45	0.1817025941074064
1105	46-47	0.19836719401696
1105	48-49	0.22652060327598633
1105	50-51	0.2512462045607009
1105	52-53	0.21551607284630592
1105	54-55	0.1452465798005136
1105	56-57	0.09330163992769513
1105	58-59	0.22674794154432476
1105	60-61	0.14267340784557803
1105	62-63	0.10298405732361005
1105	64-65	0.08723137172780326
1105	66-67	0.08655277317242138
1105	68-69	0.10471755392688209
1105	70-71	0.1984994561557727
1105	72-73	0.23488894841903374
1105	74-75	0.19173961675734574
1105	76-77	0.15154589695200826
1105	78-79	0.08402679475540964
1105	80-81	0.09923516217475736
1105	82-83	0.12025172204642587
1105	84-85	0.16640430644324766
1105	86-87	0.1430678416332931
1105	88-89	0.16032883160711364
1105	90-91	0.22980474633051884
1105	92-93	0.23096616553562654
1105	94-95	0.17861666463932835
1105	96-97	0.1062450166655502
1105	98-99	0.12460646487453175
1105	100	0.13797500520211514
1106	1	1.9482245665154316
1106	2	1.4897973229448702
1106	3	1.0469817993215003
1106	4	0.44694237297750306
1106	5	0.6803099000037776
1106	6	0.2637835396297561
1106	7	0.13608955267390854
1106	8	0.09337146793796336
1106	9	0.13233227348057852
1106	10-11	0.1221563039903728
1106	12-13	0.03410411575944039
1106	14-15	0.12658506674505077
1106	16-17	0.1858683922286275
1106	18-19	0.17783184120430207
1106	20-21	0.25368408220207783
1106	22-23	0.2354081931640195
1106	24-25	0.23636428903775908
1106	26-27	0.17900515372660664
1106	28-29	0.21346954735219725
1106	30-31	0.2091803190760686
1106	32-33	0.28046212638917467
1106	34-35	0.3067851202014964
1106	36-37	0.30986538347404036
1106	38-39	0.26801720671799956
1106	40-41	0.27583322465915217
1106	42-43	0.2420612309382193
1106	44-45	0.24771101117674732
1106	46-47	0.23441147757984027
1106	48-49	0.22018641739187217
1106	50-51	0.2954347084930049
1106	52-53	0.28679649287713005
1106	54-55	0.28777475978220934
1106	56-57	0.2616370672454096
1106	58-59	0.18231661955777412
1106	60-61	0.09861975089818031
1106	62-63	0.05554800798036297
1106	64-65	0.08227562818106549
1106	66-67	0.007615872213207808
1106	68-69	0.04928609499000913
1106	70-71	0.05448164934266231
1106	72-73	-0.004179039233939363
1106	74-75	0.06460561063805415
1106	76-77	0.01795565323653392
1106	78-79	0.04227798126865423
1106	80-81	0.06629814342892004
1106	82-83	0.004981095599219998
1106	84-85	0.07458741509392652
1106	86-87	0.008325380678154914
1106	88-89	0.008714138287018613
1106	90-91	0.012436665009143866
1106	92-93	0.03751186487499325
1106	94-95	0.0738948350391766
1106	96-97	0.12449084201790583
1106	98-99	0.05146325176768585
1106	100	0.012855465803895072
1107	1	2.947504898753028
1107	2	1.89762889543697
1107	3	1.2472345397856799
1107	4	0.5196019872608346
1107	5	0.8236219724755856
1107	6	0.399360423684783
1107	7	0.22406837297791782
1107	8	0.2118194926844339
1107	9	0.15521069652437802
1107	10-11	0.14139236719126558
1107	12-13	0.12580206497687385
1107	14-15	0.1754127320183727
1107	16-17	0.23373210401882716
1107	18-19	0.19917431918815254
1107	20-21	0.19994015270787457
1107	22-23	0.16166580806961406
1107	24-25	0.12098877442570455
1107	26-27	0.21910126725013868
1107	28-29	0.23935926818783315
1107	30-31	0.2783089506702012
1107	32-33	0.16971135644387658
1107	34-35	0.26249782125529464
1107	36-37	0.2347844997617088
1107	38-39	0.2773183827164445
1107	40-41	0.29592321694842383
1107	42-43	0.3186782851703285
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2216	74-75	0.09241348531276117
2216	76-77	0.08895227354091872
2216	78-79	0.10658625710134118
2216	80-81	0.17968496181208593
2216	82-83	0.07523604596094202
2216	84-85	0.08318889720782963
2216	86-87	0.10392935455384844
2216	88-89	-0.013067021196185635
2216	90-91	-0.07530046236213295
2216	92-93	-0.06444692558950749
2216	94-95	-0.10275963007414646
2216	96-97	-0.18934382221126356
2216	98-99	-0.19872381124949
2216	100	-0.29128880153614034
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	183.0
3	48.0
4	99.0
5	251.0
6	827.0
7	1804.0
8	6807.0
9	9075.0
10	1584.0
11	2968.0
12	2516.0
13	3395.0
14	3423.0
15	3832.0
16	4754.0
17	5834.0
18	7986.0
19	13501.0
20	29512.0
21	40280.0
22	18838.0
23	11128.0
24	10813.0
25	11308.0
26	11372.0
27	11975.0
28	14069.0
29	16215.0
30	18995.0
31	23510.0
32	30209.0
33	41917.0
34	65044.0
35	151106.0
36	220594.0
37	422055.0
38	557045.0
39	188587.0
40	1458.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	14.509601792266514	42.35944333590197	25.364500671079288	17.76645420075222
2	16.97058560080778	40.66547304477405	24.3574697387332	18.006471615684966
3	18.229629738955097	24.84214297746819	20.483421386515722	36.44480589706099
4	33.51424611840796	25.7369642787163	22.355690075227077	18.393099527648662
5	17.130961323537495	39.572124202764904	22.983476138420166	20.313438335277436
6	36.27522180326192	22.754345348938404	23.911697033513374	17.0587358142863
7	18.8568779241057	23.101179337346057	39.92758981677088	18.114352921777357
8	35.460938044711305	23.33416627776135	23.42353392026228	17.781361757265067
9	35.23227698676331	24.927465129570358	23.152326535930015	16.687931347736317
10-11	16.788648070122044	42.36662413730453	23.41348260511767	17.431245187455755
12-13	16.684648766334657	24.413295828780555	32.17308924499101	26.728966159893773
14-15	17.034626223814037	33.02253917159161	23.32841793209621	26.614416672498137
16-17	26.300856473835793	23.907167580106435	32.22237885875078	17.569597087306995
18-19	17.38984394760695	24.17684818239142	32.29149119275776	26.141816677243874
20-21	26.414589733098005	33.24732456756421	23.441078397244464	16.89700730209332
22-23	25.937825722289176	24.421003183935376	32.18668060486629	17.454490488909155
24-25	17.09726161461273	33.13936415634859	23.20640006677127	26.556974162267416
26-27	17.548654726891773	28.564387198034318	36.4264750114127	17.460483063661215
28-29	26.87278902874778	24.019538738786423	31.78330178831981	17.324370444145988
30-31	23.775355396691054	26.158000566945066	32.13967816452298	17.926965871840896
32-33	19.46837958040976	27.065977850463913	33.18463324405051	20.281009325075814
34-35	19.99299187958858	26.924236721787544	33.449880869587126	19.63289052903675
36-37	20.11551126981732	26.288190872232992	34.235551485266676	19.360746372683014
38-39	21.35486949164403	26.353692594453953	33.21402352522059	19.07741438868143
40-41	18.96785918583797	28.54676739361886	33.24284600461292	19.24252741593025
42-43	19.11613898427055	26.485116643841227	34.21675667065158	20.181987701236643
44-45	19.082132057740623	27.449163197643838	33.05235075440591	20.416353990209632
46-47	20.187241843317597	26.48158270276432	33.85107171284732	19.480103741070756
48-49	19.244201285069025	26.69151981678224	33.74283351358229	20.32144538456645
50-51	20.335400695250428	27.736960110254778	32.57900451599786	19.348634678496936
52-53	20.360724268006443	27.072081746320947	32.98128970923829	19.585904276434317
54-55	19.76557716863512	28.45874444186022	30.867962976440154	20.90771541306451
56-57	20.201505361185664	29.08577769827175	30.498844603863635	20.213872336678946
58-59	22.353775224527624	28.61673069866553	28.93351478419037	20.09597929261648
60-61	21.191388829724563	29.855107204484376	28.578073879319536	20.375430086471525
62-63	20.027115697459454	29.71321638331662	28.544488090697122	21.715179828526804
64-65	20.94143141526433	29.640111730791595	28.832335870271358	20.586120983672718
66-67	20.89744871850658	28.497194152722678	30.070672286249607	20.534684842521138
68-69	21.71218267679027	28.500600281030252	29.46420528542485	20.323011756754624
70-71	19.55148709508156	30.480652419420917	29.560541124582734	20.407319360914787
72-73	19.55262695841805	28.476091841122898	30.39808337765247	21.573197822806584
74-75	19.700607761968207	29.576561620508585	29.05175493685729	21.671075680665922
76-77	20.913762005773357	28.600103415563634	29.7361732577124	20.749961320950607
78-79	20.132932635420033	28.913382580837244	29.32665955860461	21.627025225138112
80-81	21.206927702412127	30.005394772668858	28.245375636494106	20.542301888424905
82-83	21.021826671671132	29.31486771039423	29.05269318608335	20.61061243185129
84-85	20.211248896882566	30.35959847034383	27.802605341989306	21.626547290784302
86-87	20.336855766845016	30.343103261803122	28.53652112505805	20.78351984629381
88-89	22.317642873538006	29.316080935757995	27.767935437869333	20.59834075283467
90-91	21.193932390468063	30.46640504899542	27.5706022898205	20.769060270716015
92-93	20.207913170166876	30.428512352001828	27.423925283590258	21.939649194241035
94-95	21.046536158413645	30.745740481691445	27.17790641363738	21.02981694625753
96-97	21.25024238281777	29.702773775527806	28.022151117607454	21.02483272404697
98-99	22.122409802392784	29.877709287152378	26.96978688060713	21.030094029847707
100	20.226979620794708	31.706098086058653	26.93279778669677	21.13412450644987
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	53175.0
1	29728.0
2	4369.0
3	1998.0
4	1464.5
5	1328.0
6	1349.5
7	1502.0
8	1427.0
9	1390.5
10	1505.5
11	1945.5
12	16283.5
13	18149.0
14	5272.0
15	4648.5
16	5351.0
17	6235.5
18	6944.0
19	7634.0
20	8492.0
21	9663.5
22	11180.5
23	12688.0
24	14437.5
25	16309.5
26	18149.5
27	20323.5
28	22642.5
29	24947.5
30	27284.5
31	29648.0
32	32303.5
33	35349.5
34	38928.5
35	41831.5
36	44478.0
37	47300.5
38	49101.0
39	50428.0
40	51886.5
41	53238.5
42	53971.0
43	54900.0
44	55989.0
45	57045.5
46	58382.5
47	61885.0
48	64120.5
49	71098.5
50	103472.5
51	102733.5
52	65838.5
53	52756.0
54	49765.0
55	47750.0
56	44180.0
57	40042.0
58	36082.5
59	30568.0
60	25061.0
61	20291.0
62	16032.0
63	12303.5
64	9328.0
65	6910.0
66	5020.5
67	3774.5
68	2865.0
69	2092.5
70	1526.0
71	1073.5
72	725.5
73	489.5
74	348.0
75	262.0
76	192.5
77	127.0
78	82.5
79	47.0
80	22.0
81	14.5
82	13.0
83	6.0
84	1.5
85	0.5
86	1.0
87	0.5
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.27415916295701037
2	0.001475889312372991
3	3.053564094564809E-4
4	0.0033589205040212896
5	5.089273490941348E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.2901730709236063E-4
16-17	0.0
18-19	0.0
20-21	5.089273490941348E-5
22-23	0.002697314950198914
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.002722761317653621
36-37	2.544636745470674E-5
38-39	2.2901730709236063E-4
40-41	5.089273490941348E-5
42-43	2.0357093963765393E-4
44-45	0.001348657475099457
46-47	0.0012723183727353369
48-49	0.002137494866195366
50-51	6.870519212770819E-4
52-53	4.5803461418472127E-4
54-55	3.053564094564809E-4
56-57	3.3080277691118756E-4
58-59	7.124982887317887E-4
60-61	0.0025700831129253808
62-63	1.7812457218294717E-4
64-65	7.888373910959089E-4
66-67	1.2723183727353368E-4
68-69	2.0357093963765393E-4
70-71	0.00234106580583302
72-73	1.0178546981882697E-4
74-75	1.5267820472824044E-4
76-77	0.001475889312372991
78-79	6.107128189129618E-4
80-81	0.0026464222152895005
82-83	2.2901730709236063E-4
84-85	0.0015776747821918179
86-87	0.006590609170769045
88-89	0.0025446367454706738
90-91	0.004605792509301919
92-93	0.007862927543504383
94-95	0.005674539942399603
96-97	0.0025446367454706738
98-99	4.5803461418472127E-4
100	0.0036133841785683566
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1964917.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	66.31023307431076
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.9845297754	59.6689514248903
2	6.7676415430932835	8.975277761718074
3	1.404651259162051	2.7942825714947905
4	0.5731898003316401	1.520333970232348
5	0.3010094875674361	0.9980004639087766
6	0.18392950650657897	0.7317845067416524
7	0.12222508904262705	0.567334189936149
8	0.0915903444824277	0.4858701671988955
9	0.06933903839026981	0.41380990171266346
>10	0.4674308196585899	5.564717562816904
>50	0.016357301419811177	0.7323740663935452
>100	0.012045966993682697	1.5235860792775113
>500	0.002301291627162711	1.1622971194214577
>1k	0.003375227719838642	3.611361426151652
>5k	1.5341944181084736E-4	0.6418321686153152
>10k+	2.301291627162711E-4	10.608186619489937
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	128794	6.554678899923	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	49724	2.5305903506356757	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	30032	1.5284106147995056	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7391	0.376148203715475	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5227	0.26601632537150427	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3083	0.15690230172572175	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	3006	0.1529835611376969	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2926	0.14891214234494385	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG	2150	0.10941938005523898	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2141	0.10896134544105425	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2073	0.10550063946721414	No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG	2015	0.10254886084246814	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	1990	0.10127654246973282	No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC	1985	0.10102207879518574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.002646422215289501	0.0	0.0	0.0	0.0
2	0.002697314950198914	0.0	0.0	0.0	0.0
3	0.0027482076851083276	0.0	0.0	0.0	0.0
4	0.0027482076851083276	0.0	0.0	0.0	0.0
5	0.0027482076851083276	0.0	0.0	0.0	0.0
6	0.0027482076851083276	0.0	0.0	0.0	0.0
7	0.0027482076851083276	0.0	0.0	0.0	0.0
8	0.0027482076851083276	0.0	0.0	0.0	0.0
9	0.0027482076851083276	0.0	0.0	0.0	0.0
10-11	0.0027482076851083276	0.0	0.0	0.0	0.0
12-13	0.002773654052563034	0.0	0.0	0.0	0.0
14-15	0.002824546787472448	0.0	0.0	0.0	2.5446367454706738E-5
16-17	0.003002671359655395	0.0	0.0	0.0	5.0892734909413476E-5
18-19	0.003282581401657169	0.0	0.0	0.0	5.0892734909413476E-5
20-21	0.003384366871475996	0.0	0.0	0.0	1.0178546981882695E-4
22-23	0.0034352596063854093	0.0	0.0	0.0	2.035709396376539E-4
24-25	0.00366427691347777	0.0	0.0	0.0	2.035709396376539E-4
26-27	0.0037151696483871837	0.0	0.0	0.0	2.035709396376539E-4
28-29	0.0037660623832965973	0.0	0.0	0.0	2.2901730709236063E-4
30-31	0.0038424014856607174	0.0	0.0	0.0	2.5446367454706737E-4
32-33	0.004147757895117198	0.0	0.0	0.0	2.5446367454706737E-4
34-35	0.0045294534069377996	0.0	0.0	5.0892734909413476E-5	2.5446367454706737E-4
36-37	0.005063827123486641	0.0	0.0	5.0892734909413476E-5	2.5446367454706737E-4
38-39	0.0059290036169466695	0.0	0.0	5.0892734909413476E-5	2.5446367454706737E-4
40-41	0.006895965580225526	0.0	0.0	5.0892734909413476E-5	2.5446367454706737E-4
42-43	0.007659356603866728	0.0	0.0	5.0892734909413476E-5	2.5446367454706737E-4
44-45	0.008906228609147358	0.0	0.0	5.0892734909413476E-5	2.799100420017741E-4
46-47	0.010433010656429762	0.0	0.0	5.0892734909413476E-5	3.053564094564809E-4
48-49	0.01178166813152922	0.0	0.0	5.0892734909413476E-5	3.053564094564809E-4
50-51	0.013868270262815173	0.0	0.0	5.0892734909413476E-5	3.053564094564809E-4
52-53	0.016158443333738778	0.0	0.0	5.0892734909413476E-5	3.308027769111876E-4
54-55	0.019695488409943014	0.0	0.0	5.0892734909413476E-5	3.5624914436589434E-4
56-57	0.024072263612152575	0.0	0.0	5.0892734909413476E-5	3.5624914436589434E-4
58-59	0.027889218730358586	0.0	0.0	5.0892734909413476E-5	3.5624914436589434E-4
60-61	0.03348741957039407	0.0	0.0	5.0892734909413476E-5	3.5624914436589434E-4
62-63	0.041782935360628465	0.0	0.0	5.0892734909413476E-5	3.816955118206011E-4
64-65	0.05084184217450406	0.0	0.0	5.0892734909413476E-5	4.071418792753078E-4
66-67	0.06328511585985566	0.0	0.0	5.0892734909413476E-5	4.5803461418472127E-4
68-69	0.07860382906758911	0.0	0.0	5.0892734909413476E-5	4.83480981639428E-4
70-71	0.09957163585026746	0.0	0.0	5.0892734909413476E-5	5.598200840035482E-4
72-73	0.1276135327853543	0.0	0.0	5.0892734909413476E-5	5.85266451458255E-4
74-75	0.16074470321138246	0.0	0.0	5.0892734909413476E-5	6.361591863676685E-4
76-77	0.20232406763237326	0.0	0.0	5.0892734909413476E-5	6.616055538223752E-4
78-79	0.25540519014289154	0.0	0.0	5.0892734909413476E-5	6.616055538223752E-4
80-81	0.32533180790842564	0.0	0.0	5.0892734909413476E-5	6.616055538223752E-4
82-83	0.40917758867168436	0.0	0.0	5.0892734909413476E-5	6.616055538223752E-4
84-85	0.5188259860340156	0.0	0.0	5.0892734909413476E-5	6.616055538223752E-4
86-87	0.6472792489453754	0.0	0.0	5.0892734909413476E-5	6.616055538223752E-4
88	0.755858898874609	0.0	0.0	5.0892734909413476E-5	7.124982887317887E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	2215	0.0	87.02088	1
GTGGAAT	57570	0.0	57.764263	6
TGGAATC	58110	0.0	57.227478	7
GAATCAC	58405	0.0	57.036438	9
GGAATCA	58445	0.0	56.979866	8
AGTGGAA	57755	0.0	56.96894	5
ACGAGTG	57830	0.0	56.327618	2
AACGAGT	54525	0.0	56.11213	1
CGAGTGG	58930	0.0	55.627106	3
GAGTGGA	60085	0.0	54.75335	4
AACGTGT	690	0.0	48.49346	1
CGTGTGG	880	0.0	42.19132	3
ACGTGTG	825	0.0	42.155716	2
ATCGAGT	375	0.0	41.472157	1
GAGTACT	19645	0.0	40.574436	20-21
AGTACTT	20165	0.0	40.343864	20-21
GTACTTT	19955	0.0	39.449024	22-23
TACTTTT	21015	0.0	38.398483	22-23
CATGGGG	10215	0.0	38.14027	24-25
ACTTTTT	21750	0.0	35.89038	24-25
>>END_MODULE