FastQCFastQC Report
Thu 26 May 2016
SRR2132643_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132643_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4781515
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2839675.938849925180618No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG1144752.3941156725431165No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA756151.5814025471006572No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT158670.3318404313277277No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT117180.24506877004464067No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC86380.18065403956695733No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA82430.17239305952192976No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG78480.1641320794769022No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75570.15804614227917302No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT75220.15731415670556298No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT75210.15729324283203128No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG74700.1562266352819138No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC70330.14708727254855417No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT70240.14689904768676873No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC69610.14558147365427068No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT69510.14537233491895352No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA68990.1442848134953043No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG68400.1430508949569331No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA67040.1402066081566198No Hit
ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG65680.13736232135630652No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT65050.13604474732380847No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG64600.13510362301488127No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA64050.13395335997063693No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA61750.1291431690583424No Hit
CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT61590.12880854708183495No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59740.1249394804784676No Hit
TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA59200.12381013130775496No Hit
GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT58820.12301540411354978No Hit
GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC58170.12165600233398828No Hit
CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC57680.12063122253093424No Hit
GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT52320.1094213863179348No Hit
TACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG52150.10906585046789564No Hit
GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT51180.10703720473531925No Hit
ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC51060.10678623825293866No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA49300.10310539651135675No Hit
TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG49180.10285443002897618No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT49160.10281260228191273No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT48750.087.690061
GTGGAAT1661900.049.6214646
TGGAATC1683500.048.9820067
AGTGGAA1668450.048.9815985
GGAATCA1693350.048.7081838
GAATCAC1695450.048.650639
ACGAGTG1725150.046.876422
CGAGTGG1748550.046.5178763
AACGAGT1654350.046.2618331
CGTGTGG17400.045.9172443
GAGTGGA1783350.045.886854
AACGTGT16150.045.8189161
ACGTGTG17600.043.7932622
ATCGAGT8550.042.446541
AGTACTT456650.039.6606120-21
GAGTACT449600.039.50372720-21
GTACTTT449650.038.76685722-23
TACTTTT486550.036.72993522-23
TCGAGTG10850.036.3852772
CATGGGG238900.035.35114724-25
Produced by FastQC (version 0.11.5)