##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132641_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3305971
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.46139031467608	31.0	30.0	34.0	16.0	34.0
2	31.05290851008675	33.0	31.0	34.0	25.0	34.0
3	31.82058705294148	34.0	31.0	34.0	28.0	34.0
4	35.89391558486145	37.0	35.0	37.0	35.0	37.0
5	35.631072686360525	37.0	35.0	37.0	35.0	37.0
6	36.07635759660324	37.0	36.0	37.0	35.0	37.0
7	36.119269346282834	37.0	35.0	37.0	35.0	37.0
8	36.278714483581375	37.0	37.0	37.0	35.0	37.0
9	38.18494445353574	39.0	39.0	39.0	37.0	39.0
10-11	38.23263452704213	39.0	39.0	39.0	37.0	39.0
12-13	38.20188849206481	39.0	39.0	39.0	37.0	39.0
14-15	39.731892233779426	41.0	40.0	41.0	37.5	41.0
16-17	39.60052538270904	41.0	39.5	41.0	37.0	41.0
18-19	39.39656140359368	41.0	39.0	41.0	36.0	41.0
20-21	39.289205501197685	41.0	39.0	41.0	35.0	41.0
22-23	38.82400511075264	40.5	39.0	41.0	35.0	41.0
24-25	38.54997820610042	41.0	38.5	41.0	34.5	41.0
26-27	38.56913112667958	41.0	38.5	41.0	35.0	41.0
28-29	38.454752022930634	41.0	39.0	41.0	35.0	41.0
30-31	38.246534981704315	41.0	38.0	41.0	34.5	41.0
32-33	38.112253404521695	40.0	38.0	41.0	34.0	41.0
34-35	37.98038443168437	40.0	38.0	41.0	34.0	41.0
36-37	37.72579886514431	40.0	37.5	41.0	33.5	41.0
38-39	37.32463972007014	40.0	36.0	41.0	33.0	41.0
40-41	37.19661863337579	40.0	35.0	41.0	33.0	41.0
42-43	37.082895615236794	40.0	35.0	41.0	33.0	41.0
44-45	36.89986103931341	40.0	35.0	41.0	33.0	41.0
46-47	36.769309833631326	40.0	35.0	41.0	33.0	41.0
48-49	36.59874814388874	40.0	35.0	41.0	32.0	41.0
50-51	36.310742743962365	40.0	35.0	41.0	31.0	41.0
52-53	35.75888702592975	39.0	35.0	41.0	29.0	41.0
54-55	34.729129959095225	39.0	35.0	41.0	23.5	41.0
56-57	34.34887722850563	39.0	35.0	41.0	18.5	41.0
58-59	34.1019121159865	39.0	35.0	41.0	11.5	41.0
60-61	33.83717446402282	38.5	35.0	41.0	7.5	41.0
62-63	33.63464092697728	38.0	35.0	40.0	6.0	41.0
64-65	33.28935341538084	37.5	34.0	40.0	2.0	41.0
66-67	32.80724271326034	36.5	33.5	40.0	2.0	41.0
68-69	32.528596137110696	36.0	33.5	39.0	2.0	41.0
70-71	32.2464085438136	36.0	33.5	39.0	2.0	41.0
72-73	31.947175882668056	35.0	33.0	39.0	2.0	40.5
74-75	31.765877559119545	35.0	33.0	38.0	2.0	40.0
76-77	31.454960433712213	35.0	33.0	37.0	2.0	40.0
78-79	31.087007115307422	35.0	33.0	37.0	2.0	39.0
80-81	30.742539332619675	35.0	33.0	36.0	2.0	39.0
82-83	30.306652266459686	35.0	32.0	36.0	2.0	38.0
84-85	29.86872767486466	35.0	31.0	35.5	2.0	37.0
86-87	29.779621932557788	35.0	31.5	35.0	2.0	37.0
88-89	29.537207071689377	35.0	31.5	35.0	2.0	36.5
90-91	29.298219645604874	35.0	31.0	35.0	2.0	36.0
92-93	29.189556260475364	35.0	31.5	35.0	2.0	36.0
94-95	28.907319967416534	35.0	31.0	35.0	2.0	35.5
96-97	28.5395953261538	35.0	30.5	35.0	2.0	35.0
98-99	28.404633767204853	35.0	30.0	35.0	2.0	35.0
100	28.39369099123979	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.317851341146664
1101	2	1.9859398943340025
1101	3	1.3383370827707601
1101	4	0.5913305033598206
1101	5	0.8484953311190395
1101	6	0.42219964700392154
1101	7	0.25280436282860563
1101	8	0.14886771883666938
1101	9	0.21037163130445435
1101	10-11	0.1286689835610062
1101	12-13	0.15561555717733455
1101	14-15	0.22450408608591488
1101	16-17	0.21829943263411877
1101	18-19	0.12474678938129102
1101	20-21	0.19306435433841784
1101	22-23	-0.02170747775622317
1101	24-25	-0.02168392065264868
1101	26-27	0.01998737936867201
1101	28-29	0.10498397830878758
1101	30-31	0.20902686709639795
1101	32-33	0.18916557241615806
1101	34-35	0.2282585825828889
1101	36-37	0.21433054841494226
1101	38-39	0.16331570701914444
1101	40-41	0.22873675702670226
1101	42-43	0.19767567528986518
1101	44-45	0.2500723475717308
1101	46-47	0.23505748613630573
1101	48-49	0.24325857780223004
1101	50-51	0.24653098268447593
1101	52-53	0.2610306673801617
1101	54-55	0.23829540120884474
1101	56-57	0.06481785845361543
1101	58-59	-0.02740186038901271
1101	60-61	0.03509291022626826
1101	62-63	0.02640196356425406
1101	64-65	-0.017131070990565433
1101	66-67	0.05410320455228401
1101	68-69	-0.03924208536977147
1101	70-71	-0.017794887862528697
1101	72-73	0.056027991424990375
1101	74-75	0.07022050615112008
1101	76-77	0.10223741175654766
1101	78-79	-0.014120176983251298
1101	80-81	0.0724770623626334
1101	82-83	0.09424328454578301
1101	84-85	0.14902816902428384
1101	86-87	0.05936578116682867
1101	88-89	0.050714652552176887
1101	90-91	0.1203041396969553
1101	92-93	0.12143337317126779
1101	94-95	0.11786233064416152
1101	96-97	0.02846663468226751
1101	98-99	0.14403493402653567
1101	100	0.06194605323149105
1102	1	3.2963692846872448
1102	2	1.9771430687471536
1102	3	1.2969242158904812
1102	4	0.6061751580222179
1102	5	0.8813661924129335
1102	6	0.4348484487687969
1102	7	0.28149515628351196
1102	8	0.23087032932907192
1102	9	0.2334205528397817
1102	10-11	0.23369922316576464
1102	12-13	0.18663723929528686
1102	14-15	0.2541282778064726
1102	16-17	0.3106812150115914
1102	18-19	0.21491753623818255
1102	20-21	0.19246844212531045
1102	22-23	0.1944894915349593
1102	24-25	0.14665105826877323
1102	26-27	0.2017489192072972
1102	28-29	0.24646445724544463
1102	30-31	0.2469156427370578
1102	32-33	0.27786415609563164
1102	34-35	0.2703608232394501
1102	36-37	0.2052684059157528
1102	38-39	0.2614667024089101
1102	40-41	0.31983515985609756
1102	42-43	0.2635399613824845
1102	44-45	0.2835096975686824
1102	46-47	0.22954766398981974
1102	48-49	0.2768430912878159
1102	50-51	0.36913197319788793
1102	52-53	0.3880442886048243
1102	54-55	0.3099580941320923
1102	56-57	0.27956124662798487
1102	58-59	0.2805484087798291
1102	60-61	0.28076527693810505
1102	62-63	0.26451848314476933
1102	64-65	0.30143313797616855
1102	66-67	0.36774307558130204
1102	68-69	0.3405002391022265
1102	70-71	0.3615765304108365
1102	72-73	0.2324228989389212
1102	74-75	0.3071977088339004
1102	76-77	0.41017194704217985
1102	78-79	0.4389750040062488
1102	80-81	0.4370615015659176
1102	82-83	0.47130414187382286
1102	84-85	0.42528251898360736
1102	86-87	0.46576688530414145
1102	88-89	0.44454233974898116
1102	90-91	0.4620603681441189
1102	92-93	0.4517108495041775
1102	94-95	0.4665746811269038
1102	96-97	0.5264897009685008
1102	98-99	0.512298276692551
1102	100	0.5545497270036392
1103	1	3.401779251323674
1103	2	2.072820662068821
1103	3	1.349175375700927
1103	4	0.6505046011621616
1103	5	0.9163983374534368
1103	6	0.46602078634123245
1103	7	0.3418826775260584
1103	8	0.27130547167608654
1103	9	0.25245477330962984
1103	10-11	0.19286325601753873
1103	12-13	0.2435358556845486
1103	14-15	0.25551467980466214
1103	16-17	0.32957896381202545
1103	18-19	0.28643215800717314
1103	20-21	0.2945038707006731
1103	22-23	0.3204365452971132
1103	24-25	0.30698720609841246
1103	26-27	0.37181249380575565
1103	28-29	0.44028390708176346
1103	30-31	0.5029304438625033
1103	32-33	0.4830395504568017
1103	34-35	0.6122282070492844
1103	36-37	0.5782492766509435
1103	38-39	0.5590807403883105
1103	40-41	0.6071587702233927
1103	42-43	0.6849409141755629
1103	44-45	0.6840222259770954
1103	46-47	0.5471988787952569
1103	48-49	0.6402856302226638
1103	50-51	0.6991476095829512
1103	52-53	0.7345159571304194
1103	54-55	0.6779991541733565
1103	56-57	0.6187634138157634
1103	58-59	0.6280539495737756
1103	60-61	0.6079269329745642
1103	62-63	0.609969506995867
1103	64-65	0.6681161503272577
1103	66-67	0.6795183900380835
1103	68-69	0.6348118393442874
1103	70-71	0.5289048859289878
1103	72-73	0.5505266564500602
1103	74-75	0.6110107405268614
1103	76-77	0.6135670706841907
1103	78-79	0.6111610222231398
1103	80-81	0.6233950165964863
1103	82-83	0.6643186517713779
1103	84-85	0.6057798826954439
1103	86-87	0.5752024570290146
1103	88-89	0.6225202553646163
1103	90-91	0.6816540606267196
1103	92-93	0.6132434873001493
1103	94-95	0.6255339446427435
1103	96-97	0.6905792613627781
1103	98-99	0.7200335085808831
1103	100	0.7022876406265226
1104	1	2.4972515041257424
1104	2	1.7464632832103533
1104	3	1.2026457985957641
1104	4	0.5272984927741646
1104	5	0.8454471380026192
1104	6	0.339653937032665
1104	7	0.223282818170091
1104	8	0.15184402809345698
1104	9	0.18188213024828315
1104	10-11	0.17525816018286378
1104	12-13	0.1827027333870319
1104	14-15	0.1631072425538065
1104	16-17	0.1981680969676063
1104	18-19	0.22632235659710886
1104	20-21	0.20127605352954703
1104	22-23	0.1712038348856879
1104	24-25	0.21998158801115153
1104	26-27	0.22990577826249847
1104	28-29	0.3025681273236245
1104	30-31	0.3131779853824881
1104	32-33	0.30477681470712525
1104	34-35	0.3008104632279256
1104	36-37	0.35306678299596683
1104	38-39	0.35025875450294564
1104	40-41	0.3129980875556697
1104	42-43	0.3127311215557498
1104	44-45	0.42419131449771896
1104	46-47	0.4352303838259388
1104	48-49	0.3290607399961658
1104	50-51	0.3547495029055696
1104	52-53	0.35433773128233526
1104	54-55	0.19658199113650454
1104	56-57	0.24867409508819804
1104	58-59	0.13036745147065432
1104	60-61	0.14057583007699037
1104	62-63	0.037052883931636416
1104	64-65	0.19795316963650578
1104	66-67	0.17627798745849077
1104	68-69	0.17386414727378963
1104	70-71	0.1514983364238205
1104	72-73	0.229906748343069
1104	74-75	0.21626302076009907
1104	76-77	0.2549395691249643
1104	78-79	0.2706970765580614
1104	80-81	0.23071863077139554
1104	82-83	0.2333101395285091
1104	84-85	0.19703656433344463
1104	86-87	0.16830286786865045
1104	88-89	0.22819116263024952
1104	90-91	0.2332052697747251
1104	92-93	0.15878941950637682
1104	94-95	0.30660363110980526
1104	96-97	0.3369520004890276
1104	98-99	0.3367228397961881
1104	100	0.38360900038084367
1105	1	3.328329405704011
1105	2	2.022612806035248
1105	3	1.3202472229587876
1105	4	0.6104115508538257
1105	5	0.8887300359582184
1105	6	0.39209199007795803
1105	7	0.2415285984695501
1105	8	0.20045172240797626
1105	9	0.20733086376721133
1105	10-11	0.16789301725177808
1105	12-13	0.14505666317021593
1105	14-15	0.17405409657510518
1105	16-17	0.1600385932836943
1105	18-19	0.22083204023115144
1105	20-21	0.19345014044039033
1105	22-23	0.16874488748047867
1105	24-25	0.16531868137775518
1105	26-27	0.21559249296154093
1105	28-29	0.2614603880284889
1105	30-31	0.32651320184650245
1105	32-33	0.29937615681375007
1105	34-35	0.3188095637886619
1105	36-37	0.2740727597084671
1105	38-39	0.3401519350613569
1105	40-41	0.34621988194306397
1105	42-43	0.34651580074363864
1105	44-45	0.3440100625211713
1105	46-47	0.3759725994117815
1105	48-49	0.22705565130477368
1105	50-51	0.23503466009896812
1105	52-53	0.33944317968022375
1105	54-55	0.37701402300768905
1105	56-57	0.483449627987369
1105	58-59	0.4599359290914222
1105	60-61	0.43974980436330213
1105	62-63	0.45387581497912777
1105	64-65	0.4472415028072234
1105	66-67	0.4272957819621439
1105	68-69	0.40521896940315116
1105	70-71	0.47549094801207303
1105	72-73	0.5509193726712169
1105	74-75	0.48961513868957596
1105	76-77	0.45895885075689336
1105	78-79	0.43556410780989907
1105	80-81	0.458207542185189
1105	82-83	0.3888757421960669
1105	84-85	0.4051217690332969
1105	86-87	0.41046415260493063
1105	88-89	0.42038586277218215
1105	90-91	0.5107515487707808
1105	92-93	0.4787043222409899
1105	94-95	0.5073101121933803
1105	96-97	0.5728027214244591
1105	98-99	0.6342083759339587
1105	100	0.5755296431607277
1106	1	1.6827985657597608
1106	2	1.341351547120361
1106	3	1.0066673205520722
1106	4	0.451547613218942
1106	5	0.6691424644153372
1106	6	0.25858283422243034
1106	7	0.13739805076357925
1106	8	0.12305868300376943
1106	9	0.13168993114303618
1106	10-11	0.11029594848152158
1106	12-13	0.09470296141986978
1106	14-15	0.09470119208253891
1106	16-17	0.10013406578140405
1106	18-19	0.08267144229401424
1106	20-21	0.17441188508929173
1106	22-23	0.08104752380546643
1106	24-25	0.035469904042940925
1106	26-27	0.08980538952081218
1106	28-29	0.055281175145367456
1106	30-31	0.048788298981939704
1106	32-33	0.05673952590896647
1106	34-35	0.05821311492405101
1106	36-37	0.1085012941369996
1106	38-39	0.08029800872698445
1106	40-41	0.07783515266447694
1106	42-43	0.01818762157746079
1106	44-45	0.10251489309304418
1106	46-47	0.06497482416820333
1106	48-49	0.0691842034891863
1106	50-51	0.15750354234439357
1106	52-53	0.08580517688020706
1106	54-55	0.17972819276096885
1106	56-57	0.16724574083376353
1106	58-59	0.10414177497324317
1106	60-61	0.11631693679635902
1106	62-63	0.1419832796229059
1106	64-65	0.12485534354954808
1106	66-67	0.1451615495485754
1106	68-69	0.22731260099398298
1106	70-71	0.2435847825863604
1106	72-73	0.21341237315304795
1106	74-75	0.19453366316117737
1106	76-77	0.20888589453868178
1106	78-79	0.24575598504087992
1106	80-81	0.3212864868283809
1106	82-83	0.20594375591212355
1106	84-85	0.24313883163136296
1106	86-87	0.24720467258567425
1106	88-89	0.21426351419173173
1106	90-91	0.24468047934776038
1106	92-93	0.22062296639427004
1106	94-95	0.25579194279811546
1106	96-97	0.32575097325539204
1106	98-99	0.28571759504094274
1106	100	0.29880847481944883
1107	1	2.8068857688792264
1107	2	1.8132843350248251
1107	3	1.163283835429798
1107	4	0.508514564442379
1107	5	0.8218833112946555
1107	6	0.3467507638537768
1107	7	0.14402077501022603
1107	8	0.11957510914253788
1107	9	0.06322704076826824
1107	10-11	0.10483425832274662
1107	12-13	0.08917788067842736
1107	14-15	0.1123672003464975
1107	16-17	0.12991689113857774
1107	18-19	0.11685965769780893
1107	20-21	0.13263417027852853
1107	22-23	-0.03913282692170128
1107	24-25	-0.05575641487810401
1107	26-27	-0.014975451196299616
1107	28-29	-0.0029426034364021803
1107	30-31	0.08032955473640868
1107	32-33	0.041154992425141756
1107	34-35	0.054272287851837575
1107	36-37	0.03909082655579965
1107	38-39	-9.96227524723281E-5
1107	40-41	0.015435023289526839
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2216	76-77	-0.6208587418428166
2216	78-79	-0.5336005765543312
2216	80-81	-0.6267385108699308
2216	82-83	-0.7352084034828117
2216	84-85	-0.6680087724839154
2216	86-87	-0.7462899345953176
2216	88-89	-0.9896577364970582
2216	90-91	-0.962064065653319
2216	92-93	-0.9910611541619261
2216	94-95	-1.1157590818306282
2216	96-97	-1.1426845286567264
2216	98-99	-1.1813605640764209
2216	100	-1.2004478397050917
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	577.0
3	159.0
4	238.0
5	633.0
6	1890.0
7	4045.0
8	14685.0
9	19931.0
10	4034.0
11	10130.0
12	7516.0
13	10924.0
14	10043.0
15	10781.0
16	12726.0
17	15483.0
18	19566.0
19	29100.0
20	58280.0
21	77072.0
22	37142.0
23	22313.0
24	21243.0
25	22075.0
26	23432.0
27	25840.0
28	29836.0
29	35149.0
30	40197.0
31	48385.0
32	61646.0
33	84143.0
34	124559.0
35	285941.0
36	372130.0
37	650225.0
38	800427.0
39	309366.0
40	4109.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.302844702150304	46.078932262813055	24.00388095963341	16.614342075403236
2	15.373737410402722	44.99822136395975	22.72609851949724	16.90194270614029
3	16.19068331980727	23.931695545291948	19.320815145239074	40.55680598966171
4	37.00698582326176	25.132870999728357	21.02468421017606	16.835458966833823
5	15.541222558348247	44.18247721016138	21.643820616587753	18.63247961490262
6	39.80527959864137	22.16395727609226	22.682080393324686	15.34868273194169
7	17.429614476352032	22.573519247446512	43.603014061526856	16.393852214674602
8	38.950855890750404	23.13504867405068	21.80630138618881	16.10779404901011
9	38.69241442226807	24.406233448508775	22.28821728926237	14.613134839960788
10-11	14.82292191915779	46.72714914922121	22.31078252047583	16.139146411145166
12-13	14.662167332986284	23.61595125910058	33.59985613908894	28.122025268824196
14-15	15.216567036074972	34.85016844564934	21.92155839638229	28.011706121893397
16-17	27.202945821363826	22.946919377090722	33.5203938570544	16.329740944491043
18-19	15.74528330708285	23.529849475388623	33.58338594016705	27.14148127736148
20-21	27.336808469062863	35.0028070498088	22.511904250563074	15.148480230565264
22-23	26.517563767012287	23.601080800663667	33.70071380334903	16.180641628975017
24-25	15.15975185505257	34.90785309369017	22.074739917561285	27.857655133695967
26-27	15.640624191803255	30.144502175003957	38.13406106708135	16.080812566111437
28-29	29.618408630928705	23.178954080359446	31.37503323531876	15.827604053393088
30-31	23.689363881292362	27.393540354709707	32.064240732904196	16.85285503109374
32-33	17.245208328386475	28.5087238376749	32.84477281330852	21.401295020630098
34-35	20.02495235413165	28.46191226868443	32.782656344315264	18.730479032868654
36-37	20.620864772043422	25.905698767033392	35.70016299303427	17.77327346788892
38-39	23.512976237505164	26.003836700074956	33.168247949313454	17.314939113106426
40-41	16.90096750712083	32.367908304081965	32.94905384061957	17.78207034817764
42-43	16.909444924720944	26.133874335065755	36.0098984684053	20.946782271807997
44-45	16.93119868599858	29.348633101250556	32.39391608872837	21.326252124022496
46-47	20.502004657999702	25.949677329389303	35.18377833983252	18.36453967277847
48-49	17.488687300329094	26.488528040022885	35.13536445427936	20.88742020536866
50-51	20.60139784412822	29.608678055432208	32.129770341215284	17.660153759224283
52-53	20.25687266954773	26.7604206588289	34.39187200559475	18.590834666028627
54-55	17.759084611239423	30.39043255645899	29.93484648868415	21.915636343617432
56-57	18.292186410111682	30.911161000835463	31.378045335119197	19.418607253933658
58-59	24.64809608145326	28.311511534523408	28.007997713210596	19.03239467081273
60-61	21.205159377029513	31.3933590912982	27.922174365067576	19.479307166604706
62-63	18.058930137050737	31.277489132902325	27.673201671342305	22.990379058704637
64-65	20.806723410974364	31.243494666117755	27.87840133868088	20.071380584227004
66-67	21.330295591498334	28.191908896783374	30.937794401603526	19.540001110114762
68-69	23.987284185359663	28.579124623937	28.4108528773168	19.02273831338654
70-71	17.56996335954801	34.197311541243266	28.461491226770004	19.771233872438714
72-73	17.43110788193224	28.60189729718938	31.166291486361324	22.800703334517053
74-75	17.726168043798403	31.48700432596771	27.79203231249691	22.994795317736983
76-77	21.046704445152933	28.341033618900298	30.422636480175974	20.1896254557708
78-79	18.56917851851538	28.807614987988337	29.927170795479913	22.696035698016367
80-81	21.49958725346751	31.615824783727774	27.295643580589022	19.588944382215693
82-83	21.017580062196743	29.110355506311176	29.895104630695883	19.976959800796198
84-85	18.51100733136455	31.957740825358528	26.692358859057336	22.838892984219584
86-87	18.527272515523137	32.110131567583736	29.32497406445627	20.037621852436853
88-89	24.52617390628351	28.93627981670386	26.889095791260914	19.648450485751717
90-91	21.25296255571571	31.991170184930024	26.74630843248852	20.00955882686575
92-93	18.49337103523171	31.88128590799537	26.336535574675395	23.288807482097525
94-95	20.94499164651778	32.28455260697312	26.129928335199242	20.640527411309854
96-97	21.49587002976954	29.28672427788477	28.80578046099763	20.411625231348058
98-99	24.047412025785594	29.737115256728426	26.128339031889276	20.0871336855967
100	18.464199925405484	34.742502207435074	26.267494244334248	20.525803622825194
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	122195.0
1	67958.5
2	9415.0
3	4164.5
4	3009.0
5	2663.5
6	2774.5
7	3181.5
8	2985.0
9	2671.5
10	2816.5
11	3781.0
12	30218.0
13	33068.0
14	8852.5
15	7699.5
16	8627.5
17	9843.0
18	11098.0
19	12524.5
20	14213.5
21	16236.0
22	18480.0
23	21284.0
24	24515.5
25	27770.5
26	32082.5
27	36605.5
28	39823.0
29	42493.5
30	45512.0
31	48679.5
32	52073.5
33	56110.5
34	61055.0
35	65337.5
36	68583.0
37	71550.0
38	73992.0
39	75949.0
40	78018.0
41	80456.0
42	80701.5
43	79507.5
44	79899.0
45	80966.5
46	84515.0
47	96318.0
48	105160.0
49	143259.5
50	305518.0
51	306392.0
52	131093.0
53	71222.5
54	61117.0
55	58259.5
56	52106.5
57	44112.5
58	40216.0
59	34257.0
60	26787.5
61	21286.5
62	16162.0
63	11986.0
64	8708.0
65	6455.0
66	4944.5
67	3798.0
68	2865.0
69	2163.0
70	1686.0
71	1351.0
72	1155.0
73	856.0
74	601.0
75	451.0
76	317.5
77	198.5
78	131.0
79	87.5
80	44.0
81	20.0
82	10.0
83	8.5
84	5.5
85	1.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.2988229479326951
2	0.0020266360473216493
3	2.4198639371004766E-4
4	0.004023023795429543
5	6.0496598427511915E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.5711054331692564E-4
16-17	0.0
18-19	0.0
20-21	2.722346929238036E-4
22-23	0.0026769744804174024
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	1.5124149606877979E-5
34-35	0.002132505094569795
36-37	4.5372448820633936E-5
38-39	1.512414960687798E-4
40-41	1.5124149606877979E-5
42-43	1.512414960687798E-4
44-45	0.0011645595197296043
46-47	0.0013762976142258961
48-49	0.0018905187008597473
50-51	6.95710881916387E-4
52-53	4.234761889925834E-4
54-55	2.8735884253068165E-4
56-57	3.9322788977882745E-4
58-59	7.864557795576549E-4
60-61	0.002056884346535405
62-63	1.6636564567565777E-4
64-65	9.074489764126788E-4
66-67	1.9661394488941372E-4
68-69	2.722346929238036E-4
70-71	0.0018753945512528692
72-73	1.512414960687798E-4
74-75	1.6636564567565777E-4
76-77	9.981938740539467E-4
78-79	5.142210866338513E-4
80-81	0.002056884346535405
82-83	3.024829921375596E-4
84-85	0.0011494353701227266
86-87	0.005429569708869195
88-89	0.0019358911496803813
90-91	0.003811285700933251
92-93	0.006458011882136897
94-95	0.0049607210710559775
96-97	0.002283746590638575
98-99	4.990969370269734E-4
100	0.002964333322948084
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3305971.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.18022443123352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.61406689027842	41.730851816754864
2	8.730422329734024	8.412674144520759
3	1.9577513081375537	2.82974692219825
4	0.6934769707418088	1.3364750435292923
5	0.365563373578822	0.8806462691433252
6	0.25755633530074074	0.7445473223085433
7	0.18898648045254726	0.6373787729870859
8	0.15105114511156925	0.5822142457656183
9	0.13252260655017215	0.5746472033219416
>10	0.8029231000641676	7.642246838716979
>50	0.06691780219591155	2.0825513021295055
>100	0.0294571594114939	3.0114893550370594
>500	0.0038978304287079026	1.256704117658267
>1k	0.002829070472446003	4.120568296505641
>5k	0.0021375199125147574	7.616040715161969
>10k+	4.40077629047156E-4	16.54121763426091
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	228074	6.898850594878176	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	152650	4.617402874979847	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	117237	3.546219854923107	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	14246	0.4309172705991674	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12632	0.38209651566816527	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11181	0.33820623350900536	No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC	10074	0.3047213662793775	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	9631	0.29132136972768363	No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG	9570	0.2894762234756445	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG	9519	0.28793356021574296	No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT	9301	0.2813394309871442	No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT	9211	0.27861708405790614	No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC	8872	0.26836291062444284	No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG	8785	0.2657313085928461	No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT	8569	0.2591976759626748	No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA	8543	0.25841122018311713	No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT	8535	0.25816923378940715	No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA	8388	0.253722733804985	No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC	8357	0.2527850365293586	No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA	8196	0.24791506035594385	No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA	7827	0.23675343794606787	No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT	7704	0.2330328971427759	No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG	7627	0.23070377810331671	No Hit
ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG	7571	0.22900987334734638	No Hit
TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA	7365	0.22277872370931265	No Hit
CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT	7015	0.21219181898449804	No Hit
GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT	7009	0.21201032918921553	No Hit
TACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	6968	0.21077014892145152	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6928	0.2095602169529013	No Hit
GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC	6804	0.20580942785039555	No Hit
CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC	6747	0.20408527479521144	No Hit
GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT	6336	0.19165322381835775	No Hit
ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC	6262	0.18941484967653982	No Hit
GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT	6133	0.1855128190779653	No Hit
TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG	5811	0.17577286673113587	No Hit
CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG	5589	0.16905774430568205	No Hit
ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG	5563	0.16827128852612439	No Hit
CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT	5278	0.15965052325020396	No Hit
CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG	5207	0.15750289400602727	No Hit
ACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATC	5138	0.15541576136027813	No Hit
TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC	5078	0.15360086340745277	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4989	0.15090876477742848	No Hit
TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA	4915	0.14867039063561055	No Hit
GCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCC	4829	0.1460690369032275	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	4823	0.145887547107945	No Hit
ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC	4751	0.14370966956455455	No Hit
ACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAAC	4724	0.14289296548578315	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4541	0.1373575267296658	No Hit
TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG	4456	0.13478642129649657	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGAAACGAGTGGAATCACTGCTG	4428	0.13393946891851138	No Hit
CAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCC	4318	0.13061215600499823	No Hit
GAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATG	4295	0.12991644512308184	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	4211	0.12737558798912635	No Hit
TGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACA	4055	0.12265685331178042	No Hit
GGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGC	4010	0.1212956798471614	No Hit
AGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGA	3941	0.11920854720141223	No Hit
GGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACAT	3872	0.11712141455566306	No Hit
TACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAAT	3825	0.11569974449261655	No Hit
AGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCA	3659	0.11067852682313305	No Hit
GGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTG	3606	0.10907536696480398	No Hit
TCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGA	3605	0.10904511866559022	No Hit
CACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAA	3381	0.10226949964170891	No Hit
GAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCT	3380	0.10223925134249515	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.005656431952972364	0.0	0.0	0.0	3.0248299213755958E-5
2	0.005716928551399876	0.0	0.0	0.0	3.0248299213755958E-5
3	0.0057774251498273884	0.0	0.0	0.0	3.0248299213755958E-5
4	0.0057774251498273884	0.0	0.0	0.0	3.0248299213755958E-5
5	0.0057774251498273884	0.0	0.0	0.0	3.0248299213755958E-5
6	0.0057774251498273884	0.0	0.0	0.0	6.0496598427511915E-5
7	0.005807673449041144	0.0	0.0	0.0	6.0496598427511915E-5
8	0.005807673449041144	0.0	0.0	0.0	6.0496598427511915E-5
9	0.005807673449041144	3.0248299213755958E-5	0.0	0.0	6.0496598427511915E-5
10-11	0.0058379217482549	3.0248299213755958E-5	0.0	0.0	9.074489764126787E-5
12-13	0.005943790795503046	3.0248299213755958E-5	0.0	0.0	1.8148979528253575E-4
14-15	0.0061404047403924595	3.0248299213755958E-5	0.0	0.0	2.117380944962917E-4
16-17	0.006306770386068117	3.0248299213755958E-5	0.0	0.0	2.4198639371004766E-4
18-19	0.006442887732530019	3.0248299213755958E-5	0.0	0.0	2.722346929238036E-4
20-21	0.006820991472701968	3.0248299213755958E-5	0.0	0.0	3.024829921375596E-4
22-23	0.007153722764053284	3.0248299213755958E-5	0.0	0.0	3.478554409581935E-4
24-25	0.007909930244397184	3.0248299213755958E-5	0.0	0.0	3.9322788977882745E-4
26-27	0.008590516976706692	3.0248299213755958E-5	0.0	3.0248299213755958E-5	3.9322788977882745E-4
28-29	0.009180358811374934	3.0248299213755958E-5	0.0	3.0248299213755958E-5	4.234761889925834E-4
30-31	0.009785324795650053	3.0248299213755958E-5	0.0	3.0248299213755958E-5	4.6884863781321734E-4
32-33	0.010828891118524633	3.0248299213755958E-5	0.0	3.0248299213755958E-5	5.142210866338513E-4
34-35	0.012341306079212432	3.0248299213755958E-5	0.0	4.5372448820633936E-5	5.293452362407293E-4
36-37	0.013566362197369546	3.0248299213755958E-5	0.0	6.0496598427511915E-5	5.747176850613632E-4
38-39	0.01541150844940866	3.0248299213755958E-5	0.0	6.0496598427511915E-5	6.049659842751192E-4
40-41	0.018829566260563084	3.0248299213755958E-5	0.0	6.0496598427511915E-5	6.049659842751192E-4
42-43	0.021975389378793703	3.0248299213755958E-5	0.0	6.0496598427511915E-5	6.352142834888751E-4
44-45	0.02492459855213491	3.0248299213755958E-5	0.0	6.0496598427511915E-5	6.352142834888751E-4
46-47	0.028040173371151774	3.0248299213755958E-5	0.0	6.0496598427511915E-5	6.352142834888751E-4
48-49	0.03194220396972629	3.0248299213755958E-5	0.0	6.0496598427511915E-5	6.654625827026311E-4
50-51	0.03799186381247749	3.0248299213755958E-5	0.0	6.0496598427511915E-5	6.654625827026311E-4
52-53	0.044313758348152474	3.0248299213755958E-5	0.0	6.0496598427511915E-5	6.654625827026311E-4
54-55	0.052495923285473464	3.0248299213755958E-5	0.0	6.0496598427511915E-5	6.95710881916387E-4
56-57	0.06055709502593942	3.0248299213755958E-5	0.0	9.074489764126787E-5	7.25959181130143E-4
58-59	0.06966183308927998	3.0248299213755958E-5	0.0	9.074489764126787E-5	7.25959181130143E-4
60-61	0.08103519359365222	3.0248299213755958E-5	0.0	9.074489764126787E-5	7.71331629950777E-4
62-63	0.09646182619266776	3.0248299213755958E-5	0.0	9.074489764126787E-5	8.015799291645328E-4
64-65	0.1160778482327885	3.0248299213755958E-5	0.0	9.074489764126787E-5	8.620765275920447E-4
66-67	0.13794736856433404	3.0248299213755958E-5	0.0	9.074489764126787E-5	8.772006771989228E-4
68-69	0.16244849092747637	3.0248299213755958E-5	0.0	9.074489764126787E-5	9.376972756264347E-4
70-71	0.19558550271614603	3.0248299213755958E-5	0.0	9.074489764126787E-5	9.528214252333127E-4
72-73	0.23595185801690335	3.0248299213755958E-5	0.0	9.074489764126787E-5	9.981938740539467E-4
74-75	0.2853019581841462	3.0248299213755958E-5	0.0	9.074489764126787E-5	0.0010435663228745805
76-77	0.3430610855328132	3.0248299213755958E-5	0.0	9.074489764126787E-5	0.0010586904724814586
78-79	0.4109836414173022	3.0248299213755958E-5	0.0	1.0586904724814585E-4	0.0010738146220883365
80-81	0.50507097612169	4.5372448820633936E-5	0.0	1.2099319685502383E-4	0.0011343112205158485
82-83	0.6208463413623411	6.0496598427511915E-5	0.0	1.2099319685502383E-4	0.0011494353701227264
84-85	0.761410187808665	6.0496598427511915E-5	0.0	1.2099319685502383E-4	0.0011796836693364825
86-87	0.9255374593425048	6.0496598427511915E-5	0.0	1.2099319685502383E-4	0.0011796836693364825
88	1.0620480336941855	6.0496598427511915E-5	0.0	1.2099319685502383E-4	0.0012099319685502383
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	4195	0.0	86.88154	1
ATCGAGT	650	0.0	55.12901	1
AACGTGT	1310	0.0	53.988335	1
CGTGTGG	1380	0.0	53.132904	3
ACGTGTG	1510	0.0	48.55855	2
TCGAGTG	825	0.0	45.008152	2
GTGGAAT	174410	0.0	41.324993	6
TGGAATC	175440	0.0	41.095768	7
AGTGGAA	174280	0.0	40.92975	5
GGAATCA	177065	0.0	40.739853	8
GAATCAC	177635	0.0	40.619705	9
AGTACTT	36875	0.0	38.91168	20-21
GAGTACT	36610	0.0	38.37173	20-21
ACGAGTG	184770	0.0	38.167496	2
GTACTTT	36165	0.0	37.985596	22-23
CGAGTGG	187400	0.0	37.912754	3
GAGTGGA	189765	0.0	37.673084	4
AACGAGT	178145	0.0	37.466827	1
TACTTTT	38890	0.0	36.188038	22-23
ACTTTTT	39415	0.0	34.578693	24-25
>>END_MODULE