FastQCFastQC Report
Thu 26 May 2016
SRR2132640_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132640_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3783160
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2814087.4384377081593165No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG810322.1419131096755093No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA596891.5777551041986064No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT156320.413199547468254No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT79980.2114105668277313No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT59100.15621861089671069No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC58710.1551877266623669No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA58070.15349601920087969No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56810.15016547013607673No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA56210.14857949439093246No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG55320.1462269637023018No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG54670.14450882331172882No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT54480.14400659765909982No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT53450.1412840059632688No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG52730.13938083506909568No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC51550.1362617494369786No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC51200.1353365969189778No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA49520.13089586483257382No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT49100.12978568181097283No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA47630.12590004123536935No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA47620.12587360830628364No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT46630.1232567483267956No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT46280.12233159580879477No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44630.11797016250964802No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG44300.11709787584981866No Hit
ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG43950.11617272333181783No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA43580.11519470495564554No Hit
TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA43400.11471891223210227No Hit
CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT42290.11178485710358536No Hit
GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT40120.10604891149198026No Hit
GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC39980.10567885048477992No Hit
CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC39740.1050444601867222No Hit
TACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG37980.10039226466763236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT43500.087.791721
ATCGAGT7250.059.8284261
CGTGTGG14550.057.1726343
GTGGAAT1385550.054.8566256
TGGAATC1396300.054.444397
GGAATCA1404150.054.190228
AGTGGAA1389050.054.180465
GAATCAC1406450.054.0882349
AACGTGT13950.054.075871
ACGAGTG1410500.052.6096272
AACGAGT1345000.052.3353651
CGAGTGG1434900.052.1630553
GAGTGGA1461150.051.486474
TCGAGTG8950.051.462012
ACGTGTG17200.047.2717172
AGTACTT440000.040.44645320-21
GAGTACT433000.040.1342820-21
GTACTTT432800.039.32594722-23
TACTTTT469050.037.24858522-23
CATGGGG193450.036.93842724-25
Produced by FastQC (version 0.11.5)