FastQCFastQC Report
Thu 26 May 2016
SRR2132639_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132639_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5141192
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG3110936.050989731564198No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3088336.007031054276907No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA994861.9350765347802612No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT309740.6024672877418311No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC222620.43301242202197465No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA212030.41241408607186814No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG210800.41002164478587844No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT207960.40449763401172334No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT203460.3957448000385903No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG199620.38827571504818337No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG190980.3714702738197679No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC190830.3711785126873301No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA186060.36190050867580903No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT186060.36190050867580903No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC186050.3618810579336465No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT184370.3586133332503435No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA184350.35857443176601844No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA182610.3551900026297403No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT174320.33906533737701294No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT170990.3325882402368945No Hit
ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG170480.33159625238660606No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG170300.33124613902768074No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA169830.33033195414604244No Hit
TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA164310.3195951444723325No Hit
CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT162270.31562719307117887No Hit
GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC154150.2998331904352143No Hit
GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT152540.2967016209470488No Hit
TACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG149170.2901467208382803No Hit
GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT147350.28660668576470205No Hit
CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC145230.28248312842624823No Hit
ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC137340.26713649286002156No Hit
GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT137190.2668447317275838No Hit
ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG133210.25910333634690164No Hit
CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG132320.2573722202944375No Hit
TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG130050.25295690182354597No Hit
CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT124630.24241459957146122No Hit
ACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATC122050.23739630809353163No Hit
CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG114680.2230611111197559No Hit
TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC114130.22199132030081742No Hit
TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA114070.2218746158478423No Hit
ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC112580.21897645526562712No Hit
ACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAAC110170.2142888264044603No Hit
TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG105690.20557489391565226No Hit
GAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATG102450.19927285345499643No Hit
GCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCC102220.1988254863852585No Hit
AGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGA96680.18804977522722358No Hit
CAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCC95950.18662987104935974No Hit
GGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGC95630.1860074473001592No Hit
TACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAAT92480.17988046351896603No Hit
TGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACA91650.17826605191947703No Hit
GGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACAT90740.1764960343826879No Hit
TCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGA86970.16916310458741865No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85860.16700407220737915No Hit
AACGAGTGGAATCACTGCTGAGTACATGGAAACGAGTGGAATCACTGCTG85140.16560361877167787No Hit
GGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTG84610.1645727294370644No Hit
AGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCA80700.15696748925151988No Hit
GAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGG78910.15348580640442916No Hit
CACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAA78490.15266887523360342No Hit
TGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACT77490.15072380101735164No Hit
GAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCT76840.14945950277678796No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT70070.1362913503327633No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT62820.12218956226493778No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA61950.12049734769679872No Hit
GTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTAC60620.11791039898918383No Hit
GCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAG58830.11442871614209313No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGTAACGAGTGGAATCACTGCT55380.10771821009602442No Hit
GTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAA54990.10695963115168622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT67550.086.7959441
CGTGTGG23000.060.076653
AACGTGT22200.059.8687971
ACGTGTG24250.055.818152
ATCGAGT11050.052.0357671
GTGTGGA32250.045.4685484
TCGAGTG13850.043.7758522
AGTACTT512650.038.56969520-21
GAGTACT509850.038.0763120-21
GTACTTT499600.037.71791522-23
TACTTTT518400.037.14786522-23
AGCGAGT11100.036.9403231
TACGGCG2750.035.888556
ACTTTTT533850.034.98468424-25
GTGGAAT3377350.034.9733776
TGGAATC3403400.034.705697
ATCTGCG6800.034.55957894
AGTGGAA3379400.034.5001875
GGAATCA3432400.034.4384778
GAATCAC3440400.034.3665969
Produced by FastQC (version 0.11.5)