##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132639_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5141192
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.465287427507082	31.0	30.0	34.0	16.0	34.0
2	31.054924422196255	33.0	31.0	34.0	25.0	34.0
3	31.82999273320273	34.0	31.0	34.0	28.0	34.0
4	35.89852042094518	37.0	35.0	37.0	35.0	37.0
5	35.63428461726386	37.0	35.0	37.0	35.0	37.0
6	36.08350417568533	37.0	36.0	37.0	35.0	37.0
7	36.12399420212277	37.0	35.0	37.0	35.0	37.0
8	36.283973249783315	37.0	37.0	37.0	35.0	37.0
9	38.18435160561987	39.0	39.0	39.0	37.0	39.0
10-11	38.231493007847206	39.0	39.0	39.0	37.0	39.0
12-13	38.21053697274873	39.0	39.0	39.0	37.0	39.0
14-15	39.75075352175138	41.0	40.0	41.0	37.5	41.0
16-17	39.657283272050535	41.0	40.0	41.0	37.0	41.0
18-19	39.560058445590045	41.0	40.0	41.0	36.5	41.0
20-21	39.50027590877758	41.0	39.5	41.0	36.0	41.0
22-23	39.191870679017626	40.5	39.0	41.0	36.0	41.0
24-25	38.84863568604324	41.0	39.0	41.0	34.5	41.0
26-27	38.9021597520575	41.0	39.0	41.0	35.0	41.0
28-29	38.827271477120476	41.0	39.0	41.0	35.0	41.0
30-31	38.61711408560505	41.0	39.0	41.0	35.0	41.0
32-33	38.502900883686124	41.0	38.0	41.0	35.0	41.0
34-35	38.35707546032126	40.5	38.0	41.0	35.0	41.0
36-37	38.086082274305255	40.0	38.0	41.0	34.0	41.0
38-39	37.733836530516655	40.0	36.5	41.0	34.0	41.0
40-41	37.644725581149274	40.0	36.0	41.0	33.0	41.0
42-43	37.553467172593436	40.0	36.0	41.0	33.0	41.0
44-45	37.37652328487246	40.0	36.0	41.0	33.0	41.0
46-47	37.26424358397819	40.0	35.0	41.0	33.0	41.0
48-49	37.10202847899865	40.0	35.0	41.0	33.0	41.0
50-51	36.82078737771319	40.0	35.0	41.0	32.5	41.0
52-53	36.270673415814855	39.5	35.0	41.0	30.5	41.0
54-55	35.22516519515318	39.0	35.0	41.0	26.5	41.0
56-57	34.92998189913934	39.0	35.0	41.0	23.0	41.0
58-59	34.729684964109495	39.0	35.0	41.0	21.0	41.0
60-61	34.47212300182526	39.0	35.0	41.0	18.5	41.0
62-63	34.303351635184995	39.0	35.0	41.0	18.0	41.0
64-65	33.92020264950229	38.0	35.0	40.0	14.0	41.0
66-67	33.365800771494236	37.5	34.0	40.0	10.0	41.0
68-69	33.082089231446716	37.0	34.0	39.5	7.5	41.0
70-71	32.78852997514973	36.5	34.0	39.0	6.0	41.0
72-73	32.469140522275765	36.0	34.0	39.0	2.0	41.0
74-75	32.273109426763284	36.0	34.0	39.0	2.0	40.0
76-77	31.94422655290835	35.0	34.0	37.5	2.0	40.0
78-79	31.52785900623824	35.0	33.0	37.0	2.0	39.0
80-81	31.146190319287825	35.0	33.0	37.0	2.0	39.0
82-83	30.63296770476574	35.0	32.5	36.0	2.0	38.0
84-85	30.155990381218984	35.0	31.0	36.0	2.0	37.0
86-87	30.07779791145711	35.0	31.5	35.5	2.0	37.0
88-89	29.82647992916818	35.0	32.0	35.0	2.0	36.5
90-91	29.558630858369032	35.0	31.5	35.0	2.0	36.0
92-93	29.45656941036242	35.0	31.5	35.0	2.0	36.0
94-95	29.14549612230004	35.0	31.0	35.0	2.0	35.5
96-97	28.72692043012593	35.0	30.5	35.0	2.0	35.0
98-99	28.594540332280918	35.0	30.0	35.0	2.0	35.0
100	28.606291692665824	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.3502037040334223
1101	2	2.025280088162745
1101	3	1.3236928229500329
1101	4	0.5827786722407708
1101	5	0.8374338034846858
1101	6	0.38186250939418187
1101	7	0.2213766722354933
1101	8	0.1703775580443505
1101	9	0.2100674476854536
1101	10-11	0.11996983964863261
1101	12-13	0.1564650094772162
1101	14-15	0.2489881452769822
1101	16-17	0.22067739016224408
1101	18-19	0.09311351188870987
1101	20-21	0.1905875513083899
1101	22-23	0.1369813146328127
1101	24-25	0.1678221000345559
1101	26-27	0.2056803355369965
1101	28-29	0.18999378718422832
1101	30-31	0.19247544287081553
1101	32-33	0.19263532091532198
1101	34-35	0.17256391810627747
1101	36-37	0.21136506957686407
1101	38-39	0.21185169168824558
1101	40-41	0.2744952267881118
1101	42-43	0.26673502802788107
1101	44-45	0.30196188821592784
1101	46-47	0.2645006179526135
1101	48-49	0.29117311235234666
1101	50-51	0.31533244004218375
1101	52-53	0.3730125231202095
1101	54-55	0.34899967929757736
1101	56-57	0.27983843299850975
1101	58-59	0.15539211952820153
1101	60-61	0.23029333651779638
1101	62-63	0.2561609637460407
1101	64-65	0.3155124404295506
1101	66-67	0.3293177343228635
1101	68-69	0.2829485951865749
1101	70-71	0.3144747964198942
1101	72-73	0.26335574484209445
1101	74-75	0.33443681024226635
1101	76-77	0.3697657060927071
1101	78-79	0.20782082751593833
1101	80-81	0.27685972363454425
1101	82-83	0.23694081836637437
1101	84-85	0.2628606454734026
1101	86-87	0.20668218933867166
1101	88-89	0.3031326817623139
1101	90-91	0.39949860984825847
1101	92-93	0.395361309432154
1101	94-95	0.39693548893071195
1101	96-97	0.42537557263099046
1101	98-99	0.47470209176783484
1101	100	0.4436356857684842
1102	1	3.3840003268014236
1102	2	2.031348762379018
1102	3	1.3310252035235202
1102	4	0.6311759317376797
1102	5	0.904528054806498
1102	6	0.4203370843469898
1102	7	0.2625257666298566
1102	8	0.20615596682157644
1102	9	0.1858030904084842
1102	10-11	0.19034918680514323
1102	12-13	0.18028655729268905
1102	14-15	0.18520880384993177
1102	16-17	0.2930390450503211
1102	18-19	0.15650074577949624
1102	20-21	0.20943621255938893
1102	22-23	0.19357296331903484
1102	24-25	0.13216024402726845
1102	26-27	0.21660926120826218
1102	28-29	0.21369185755147413
1102	30-31	0.27899414718572046
1102	32-33	0.3264024616283976
1102	34-35	0.33967031132973347
1102	36-37	0.27369037085253467
1102	38-39	0.23393945183560305
1102	40-41	0.26428157295411125
1102	42-43	0.2418038242323277
1102	44-45	0.38004740628526434
1102	46-47	0.3447612723290163
1102	48-49	0.3192065435607887
1102	50-51	0.3814516112890942
1102	52-53	0.33745538152875554
1102	54-55	0.2404782745478542
1102	56-57	0.14859971998696153
1102	58-59	0.08489836340626766
1102	60-61	0.1379488529346915
1102	62-63	0.1686934077609692
1102	64-65	0.19764529066696923
1102	66-67	0.10091573948431432
1102	68-69	0.11306163059938257
1102	70-71	0.12478066230537621
1102	72-73	0.08375836081479804
1102	74-75	0.14985169427856704
1102	76-77	0.17217870035585037
1102	78-79	0.18290041862981354
1102	80-81	0.2281566305040741
1102	82-83	0.24777599527672578
1102	84-85	0.20660113471235775
1102	86-87	0.264021181557041
1102	88-89	0.3169290977385515
1102	90-91	0.3111257233217266
1102	92-93	0.3401707792806796
1102	94-95	0.3514640008666774
1102	96-97	0.3416489270570864
1102	98-99	0.3751208735442795
1102	100	0.4273301932464726
1103	1	3.3923254085834635
1103	2	2.0454407616748895
1103	3	1.309894997711499
1103	4	0.6044902314927043
1103	5	0.8853107419516775
1103	6	0.44065093668456257
1103	7	0.32986190915799085
1103	8	0.26897997773562565
1103	9	0.27884831624427164
1103	10-11	0.2179017583552607
1103	12-13	0.24503290460598492
1103	14-15	0.28045315978884844
1103	16-17	0.29942712100859836
1103	18-19	0.22897641287157455
1103	20-21	0.2588564952392076
1103	22-23	0.2430378043758168
1103	24-25	0.2709120960421103
1103	26-27	0.32614007800181355
1103	28-29	0.31015460088055846
1103	30-31	0.35267729128347725
1103	32-33	0.2910574917740618
1103	34-35	0.41661520196917223
1103	36-37	0.3823166342567248
1103	38-39	0.4536879812257908
1103	40-41	0.483358792804772
1103	42-43	0.44492584450546957
1103	44-45	0.5019979996634873
1103	46-47	0.449485377127381
1103	48-49	0.4854677840073265
1103	50-51	0.5287382261726989
1103	52-53	0.522289396990324
1103	54-55	0.5523666512293417
1103	56-57	0.5476311712331281
1103	58-59	0.5225233285663862
1103	60-61	0.4796528349499809
1103	62-63	0.50132306713693
1103	64-65	0.4889705264779707
1103	66-67	0.5030249276783536
1103	68-69	0.6021336428043398
1103	70-71	0.5664483374949825
1103	72-73	0.6025017595737623
1103	74-75	0.5860088918317103
1103	76-77	0.6209353749440041
1103	78-79	0.6174397620263647
1103	80-81	0.5927741619333133
1103	82-83	0.6224358479957033
1103	84-85	0.5932228029193141
1103	86-87	0.5959376318496936
1103	88-89	0.6217040871032182
1103	90-91	0.6696666290119957
1103	92-93	0.6036321766900592
1103	94-95	0.6540584719605782
1103	96-97	0.6963676225051643
1103	98-99	0.7449309228335501
1103	100	0.7506962027229207
1104	1	2.4112196618290476
1104	2	1.684188469086692
1104	3	1.1748619125171196
1104	4	0.5214543951924924
1104	5	0.8138602079134856
1104	6	0.3461802997945753
1104	7	0.1998650558476669
1104	8	0.17770907472559827
1104	9	0.17032384398908818
1104	10-11	0.13744400491188458
1104	12-13	0.1262316585168719
1104	14-15	0.10162669522956946
1104	16-17	0.15405936289013766
1104	18-19	0.14880752554104504
1104	20-21	0.14057589143440907
1104	22-23	0.12770816615522307
1104	24-25	0.0873052704226609
1104	26-27	0.1439541625848335
1104	28-29	0.24120357236255785
1104	30-31	0.2864546225310036
1104	32-33	0.21963730081494504
1104	34-35	0.2670228780938899
1104	36-37	0.22914310780655
1104	38-39	0.23079833113768444
1104	40-41	0.27018063622549704
1104	42-43	0.2615977256864639
1104	44-45	0.28881031310973526
1104	46-47	0.2705184803028473
1104	48-49	0.2151963595458568
1104	50-51	0.23927806326408074
1104	52-53	0.3584209466001411
1104	54-55	0.3411291480826435
1104	56-57	0.35125417982808216
1104	58-59	0.3320887707212421
1104	60-61	0.3486332625401545
1104	62-63	0.1896005242520431
1104	64-65	0.3278510241943664
1104	66-67	0.3537377427968522
1104	68-69	0.36821017029741654
1104	70-71	0.2682602463668431
1104	72-73	0.3365104174656679
1104	74-75	0.34238606304759855
1104	76-77	0.39090925741963645
1104	78-79	0.4793717395859787
1104	80-81	0.3866995224540446
1104	82-83	0.4334257965698036
1104	84-85	0.42330524618899545
1104	86-87	0.4314902804633505
1104	88-89	0.43865759701079554
1104	90-91	0.4654844087077592
1104	92-93	0.48128924145792595
1104	94-95	0.504515281397051
1104	96-97	0.5183810993275451
1104	98-99	0.5558062869504958
1104	100	0.5080687168380749
1105	1	3.2799969598511645
1105	2	1.9595603704877078
1105	3	1.2654038819157911
1105	4	0.5777374364503629
1105	5	0.8425859895345269
1105	6	0.37148046669276624
1105	7	0.2093633662261638
1105	8	0.13498621393154764
1105	9	0.14735496804689063
1105	10-11	0.11410666677510761
1105	12-13	0.12761385885292498
1105	14-15	0.18573889113788766
1105	16-17	0.16016556853946895
1105	18-19	0.15643443834162696
1105	20-21	0.16217351688288062
1105	22-23	0.10827143994951172
1105	24-25	0.0974568257999735
1105	26-27	0.14359286123952586
1105	28-29	0.16236382113255132
1105	30-31	0.2542930845474345
1105	32-33	0.251351694165443
1105	34-35	0.23956635700653095
1105	36-37	0.19887472803995365
1105	38-39	0.21652235252810925
1105	40-41	0.24084973676981036
1105	42-43	0.2515905344209628
1105	44-45	0.24929830239280193
1105	46-47	0.24720682215344425
1105	48-49	0.20255541834607982
1105	50-51	0.23821062744340793
1105	52-53	0.16957838404155723
1105	54-55	0.1580884116275456
1105	56-57	0.20041081167396868
1105	58-59	0.3135585437249233
1105	60-61	0.2064464834319395
1105	62-63	0.184140550488209
1105	64-65	0.23558183014187506
1105	66-67	0.19073301915653218
1105	68-69	0.2011164192247037
1105	70-71	0.21926228295061634
1105	72-73	0.26534324683105837
1105	74-75	0.2375995828005415
1105	76-77	0.22632922515236942
1105	78-79	0.18604153188223904
1105	80-81	0.24477275864953185
1105	82-83	0.2404280730072621
1105	84-85	0.2112265880834805
1105	86-87	0.20270948892010665
1105	88-89	0.2640135733434903
1105	90-91	0.31706224188169685
1105	92-93	0.30477750492156375
1105	94-95	0.30848344342690837
1105	96-97	0.30828150355922546
1105	98-99	0.32828024305466386
1105	100	0.2745604264604218
1106	1	1.7962368346723068
1106	2	1.377849944487295
1106	3	1.0353177529729614
1106	4	0.46637134619639653
1106	5	0.6910895807950368
1106	6	0.2541212477355188
1106	7	0.1511892228240086
1106	8	0.12004187879752948
1106	9	0.1597483003700617
1106	10-11	0.14702847840940336
1106	12-13	0.06732880613575531
1106	14-15	0.10091512017017124
1106	16-17	0.11153365992964837
1106	18-19	0.09712120863733276
1106	20-21	0.12790559876410867
1106	22-23	0.057842958898710606
1106	24-25	0.08204722733853487
1106	26-27	0.08298212335192545
1106	28-29	0.1122069168441655
1106	30-31	0.14721765981578727
1106	32-33	0.1423240343712493
1106	34-35	0.09400766464388965
1106	36-37	0.19496644710815758
1106	38-39	0.1771821244780014
1106	40-41	0.16863316598237788
1106	42-43	0.14914566888658243
1106	44-45	0.2245606819035828
1106	46-47	0.22472025276548635
1106	48-49	0.25516235168576884
1106	50-51	0.2537556146981501
1106	52-53	0.1273269520278788
1106	54-55	0.08920353746138687
1106	56-57	0.140446211628813
1106	58-59	0.15584731491456694
1106	60-61	0.17269235964273122
1106	62-63	0.15010748910683702
1106	64-65	0.16965704426126393
1106	66-67	0.1797170452978989
1106	68-69	0.2234142737623941
1106	70-71	0.20322235350287343
1106	72-73	0.14292078648472994
1106	74-75	0.16314752380905162
1106	76-77	0.16727401987135693
1106	78-79	0.12019445953913177
1106	80-81	0.19770613259109027
1106	82-83	0.1942076395255583
1106	84-85	0.23897070794141229
1106	86-87	0.18402623082538838
1106	88-89	0.24783037270530883
1106	90-91	0.27883001640119076
1106	92-93	0.24385199271632985
1106	94-95	0.27109118536425925
1106	96-97	0.29415061161879663
1106	98-99	0.28963149555831436
1106	100	0.30963870884284717
1107	1	2.8329556565503147
1107	2	1.8285902898127517
1107	3	1.1924996470563336
1107	4	0.524524728240479
1107	5	0.8318999509861982
1107	6	0.387847191438901
1107	7	0.21670843198824485
1107	8	0.15480349240095848
1107	9	0.08276769669104311
1107	10-11	0.14141148739506804
1107	12-13	0.12018367203900482
1107	14-15	0.15449760863608475
1107	16-17	0.2073803937182248
1107	18-19	0.1737936286288857
1107	20-21	0.16339267605365393
1107	22-23	0.07558478505116994
1107	24-25	0.09375158474357193
1107	26-27	0.13016268250290608
1107	28-29	0.12038806885835385
1107	30-31	0.14155286510611376
1107	32-33	0.09773346157812313
1107	34-35	0.17787828229490543
1107	36-37	0.19396117354082776
1107	38-39	0.15437067179690445
1107	40-41	0.11449758308018687
1107	42-43	0.16595619140029072
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2216	100	-1.2962791913403642
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	746.0
3	194.0
4	257.0
5	585.0
6	1796.0
7	3523.0
8	12191.0
9	16436.0
10	4635.0
11	12482.0
12	10351.0
13	15439.0
14	14184.0
15	14928.0
16	17933.0
17	21943.0
18	28618.0
19	43340.0
20	87076.0
21	115372.0
22	55818.0
23	33726.0
24	32034.0
25	34078.0
26	36210.0
27	40273.0
28	46276.0
29	54373.0
30	63448.0
31	76182.0
32	96752.0
33	129180.0
34	188423.0
35	372497.0
36	568553.0
37	1052581.0
38	1315634.0
39	517773.0
40	5352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	13.65650986821314	45.66064708136077	23.572341536231047	17.110501514195043
2	15.663794463343278	44.292106170701814	22.282161639192417	17.761937726762493
3	16.61613996486407	22.280699979926773	18.631904451432902	42.47125560377626
4	38.20247951567437	23.713364159996765	20.518910314016484	17.565246010312382
5	16.074060674668704	43.50893470188809	20.92925956831006	19.48774505513315
6	41.21141556277222	20.662231638110384	22.15980262942913	15.966550169688274
7	17.974683692030954	21.120374419006332	43.94733750460983	16.95760438435289
8	40.593212624620904	21.69144820889786	21.089155977835492	16.626183188645747
9	40.33325345561885	22.840870366249693	21.932890271361195	14.892985906770258
10-11	15.258562605714785	46.22421220604093	21.72003107450568	16.797194113738602
12-13	15.040655941268094	22.076981369301127	33.578954063571246	29.30340862585953
14-15	15.627725234243107	33.926886332561565	21.12018305502047	29.32520537817486
16-17	28.27734985039453	21.346988021642467	33.29911621611266	17.076545911850353
18-19	16.362102053580383	22.049976158250477	33.30846555706007	28.279456231109073
20-21	28.4512532581288	33.99346395121975	21.867832080167595	15.68745071048385
22-23	27.394075469951172	22.01096037280984	33.6856402932777	16.909323863961287
24-25	15.522985720043133	33.898539482672504	21.520602615113383	29.057872182170986
26-27	15.946097714304386	29.688202658060618	37.59136013593734	16.774339491697646
28-29	32.106474529642156	21.615629215948363	29.829278890965366	16.44861736344412
30-31	24.903835530748513	26.77416054486975	30.674082975310007	17.647920949071732
32-33	17.627332338492707	27.770145522672564	31.28453479270955	23.31798734612518
34-35	21.255879843169172	27.800369221959347	31.3442250763993	19.599525858472177
36-37	22.15567039495432	24.304511631014574	35.118954475418576	18.42086349861253
38-39	25.969815136040598	24.708617816978418	31.71507508189745	17.606491965083542
40-41	17.25005769576955	32.914918652611945	31.431400678227995	18.403622973390508
42-43	17.022123118356223	24.890545619558768	35.512390825224465	22.574940436860544
44-45	17.158462111816704	28.98800613406241	30.79104430000576	23.06248745411513
46-47	21.73341649283148	24.58472849665477	34.64823697617126	19.033618034342485
48-49	17.93916619611137	25.05609220147716	34.49139861223955	22.513342990171928
50-51	22.0124886406782	29.089637886605956	30.83413017160356	18.063743301112282
52-53	21.456718107516174	25.429327508470816	33.95866318627242	19.155291197740596
54-55	18.025189754681687	29.88309584720621	28.57496166977312	23.516752728338986
56-57	18.433068620875847	30.560488495239223	31.03741245193707	19.969030431947864
58-59	26.856554106269197	26.868672015274402	26.74725951445503	19.52751436400137
60-61	22.23399452526313	31.02124285824602	26.742307874101673	20.00245474238918
62-63	18.378422957424323	30.701084273056424	26.29853050458294	24.621962264936315
64-65	21.749148488675594	30.593370786669837	26.663396323742944	20.994084400911625
66-67	22.555771696903225	26.78867879241023	30.628864990800754	20.026684519885794
68-69	26.028913753663673	27.13854087961991	27.36442258999941	19.46812277671701
70-71	17.842356863670744	34.52154520255592	27.38750845759938	20.248589476173954
72-73	17.578111589610685	27.114694244217752	31.06094283877856	24.246251327393008
74-75	17.803574445174604	30.97625559221266	26.60652753608868	24.613642426524056
76-77	22.12705191820988	26.88435791749856	29.985259090187196	21.003331074104363
78-79	18.895992666248667	27.411062655783137	29.53485316499005	24.158091512978146
80-81	22.70428676298102	31.07268080921487	26.257867507267925	19.965164920536182
82-83	22.12532601932279	27.576406052848146	29.656880527302764	20.6413874005263
84-85	18.55407384412017	31.29707746958984	25.704694709934223	24.444153976355768
86-87	18.6819043758309	31.414367930511595	29.18706724997722	20.716660443680286
88-89	26.369039103371016	27.43463263593427	25.90488552158852	20.291442739106202
90-91	22.14432080479746	31.16438812477138	25.922391501760224	20.768899568670935
92-93	18.616389649766386	31.099107601980734	25.248863919636033	25.035638828616847
94-95	21.850175079832184	31.5478753352621	24.95154339950054	21.65040618540518
96-97	22.80238047791038	27.85294535562014	28.305818138777962	21.03885602769152
98-99	26.114606319773827	28.23470944815037	25.121329504110452	20.529354727965355
100	18.785329718754966	34.7323777147511	25.278168803818218	21.20412376267571
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	100745.0
1	56031.0
2	7868.0
3	3644.0
4	2705.5
5	2395.5
6	2460.0
7	2814.5
8	2691.0
9	2479.5
10	2690.5
11	3598.5
12	26212.0
13	29531.5
14	10051.0
15	9799.5
16	11243.0
17	12984.0
18	15071.0
19	17315.0
20	19717.0
21	22689.0
22	26120.0
23	29956.0
24	34253.5
25	39080.0
26	44196.5
27	49976.0
28	55716.5
29	60457.0
30	65849.5
31	71523.5
32	77039.5
33	82804.5
34	89583.0
35	95764.5
36	100986.5
37	105848.5
38	109881.5
39	113289.5
40	114950.0
41	116010.0
42	116677.0
43	116350.0
44	117576.5
45	121256.5
46	130824.5
47	158245.5
48	181372.5
49	274324.0
50	625811.0
51	624866.0
52	247192.5
53	112489.5
54	87619.0
55	78328.0
56	69040.0
57	59225.0
58	49706.0
59	39819.5
60	31065.0
61	24363.5
62	18805.0
63	14282.5
64	10936.5
65	8178.5
66	6113.5
67	4750.0
68	3723.5
69	2973.5
70	2130.5
71	1460.0
72	1135.5
73	821.5
74	649.0
75	514.5
76	333.5
77	214.0
78	138.5
79	83.0
80	47.0
81	36.5
82	26.5
83	14.5
84	8.5
85	4.5
86	3.0
87	1.5
88	1.5
89	1.5
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.29246135915561994
2	0.0014588056621888464
3	3.1121187460028725E-4
4	0.0036567395265533756
5	3.8901484325035907E-5
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.0697908189384874E-4
16-17	5.8352226487553856E-5
18-19	9.725371081258977E-6
20-21	2.4313427703147441E-4
22-23	0.002450793512477262
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.002159032380039493
36-37	4.862685540629488E-5
38-39	1.1670445297510771E-4
40-41	9.725371081258977E-6
42-43	1.7505667946266158E-4
44-45	0.001244847498401149
46-47	0.0013129250959699617
48-49	0.0020034264427393495
50-51	7.780296865007181E-4
52-53	2.3340890595021543E-4
54-55	2.820357613565103E-4
56-57	3.1121187460028725E-4
58-59	7.974804286632361E-4
60-61	0.002392441285989708
62-63	1.0697908189384874E-4
64-65	7.585789443382002E-4
66-67	1.7505667946266158E-4
68-69	1.0697908189384874E-4
70-71	0.0020520532981456443
72-73	1.0697908189384874E-4
74-75	4.862685540629488E-5
76-77	0.0011184176743447823
78-79	5.932476359567976E-4
80-81	0.002139581637876975
82-83	1.7505667946266158E-4
84-85	0.001264298240563667
86-87	0.005942201730649235
88-89	0.0022076592354457877
90-91	0.004308339388997726
92-93	0.007401007392838081
94-95	0.004891861653873265
96-97	0.0024021666570709674
98-99	5.251700383879848E-4
100	0.003092668003840355
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5141192.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.957425340735576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.8947951249078	38.616088389858206
2	9.332488148724396	8.391292783791531
3	2.1699084433253755	2.9266049051109695
4	0.7354851334187196	1.322620718995721
5	0.351475692063925	0.7900721092523636
6	0.22125507269113442	0.596823504706242
7	0.16278615170174862	0.5122912383145913
8	0.12295851858004544	0.44223187352558735
9	0.11137549794925078	0.4506440070456621
>10	0.7936382799841945	6.918083458200757
>50	0.06351121630385939	1.8489980722395574
>100	0.028996698456854986	2.6945126535168566
>500	0.005337550574820118	1.647411056604801
>1k	0.003081025098331027	2.216512421380315
>5k	9.546838332856705E-4	3.361036139519783
>10k+	0.0019527623862661443	27.264776667937074
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	311093	6.050989731564198	No Hit
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	308833	6.007031054276907	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	99486	1.9350765347802612	No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT	30974	0.6024672877418311	No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC	22262	0.43301242202197465	No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA	21203	0.41241408607186814	No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG	21080	0.41002164478587844	No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT	20796	0.40449763401172334	No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT	20346	0.3957448000385903	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGTAACGAGTGGAATCACTGCTG	19962	0.38827571504818337	No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG	19098	0.3714702738197679	No Hit
ACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTC	19083	0.3711785126873301	No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA	18606	0.36190050867580903	No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT	18606	0.36190050867580903	No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC	18605	0.3618810579336465	No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT	18437	0.3586133332503435	No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA	18435	0.35857443176601844	No Hit
ATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTA	18261	0.3551900026297403	No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT	17432	0.33906533737701294	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	17099	0.3325882402368945	No Hit
ACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAG	17048	0.33159625238660606	No Hit
CGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAG	17030	0.33124613902768074	No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA	16983	0.33033195414604244	No Hit
TGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCA	16431	0.3195951444723325	No Hit
CAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTT	16227	0.31562719307117887	No Hit
GTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCAC	15415	0.2998331904352143	No Hit
GATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGT	15254	0.2967016209470488	No Hit
TACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	14917	0.2901467208382803	No Hit
GAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGT	14735	0.28660668576470205	No Hit
CTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGC	14523	0.28248312842624823	No Hit
ATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCC	13734	0.26713649286002156	No Hit
GTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCAT	13719	0.2668447317275838	No Hit
ATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGG	13321	0.25910333634690164	No Hit
CTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACG	13232	0.2573722202944375	No Hit
TGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATG	13005	0.25295690182354597	No Hit
CTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGT	12463	0.24241459957146122	No Hit
ACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATC	12205	0.23739630809353163	No Hit
CGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAG	11468	0.2230611111197559	No Hit
TTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTAC	11413	0.22199132030081742	No Hit
TGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGA	11407	0.2218746158478423	No Hit
ATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCAC	11258	0.21897645526562712	No Hit
ACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAAC	11017	0.2142888264044603	No Hit
TGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTG	10569	0.20557489391565226	No Hit
GAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATG	10245	0.19927285345499643	No Hit
GCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCC	10222	0.1988254863852585	No Hit
AGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGA	9668	0.18804977522722358	No Hit
CAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCC	9595	0.18662987104935974	No Hit
GGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGC	9563	0.1860074473001592	No Hit
TACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAAT	9248	0.17988046351896603	No Hit
TGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACA	9165	0.17826605191947703	No Hit
GGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACAT	9074	0.1764960343826879	No Hit
TCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGA	8697	0.16916310458741865	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8586	0.16700407220737915	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGAAACGAGTGGAATCACTGCTG	8514	0.16560361877167787	No Hit
GGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTG	8461	0.1645727294370644	No Hit
AGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCA	8070	0.15696748925151988	No Hit
GAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGG	7891	0.15348580640442916	No Hit
CACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAA	7849	0.15266887523360342	No Hit
TGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACT	7749	0.15072380101735164	No Hit
GAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCT	7684	0.14945950277678796	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7007	0.1362913503327633	No Hit
CGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6282	0.12218956226493778	No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	6195	0.12049734769679872	No Hit
GTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTAC	6062	0.11791039898918383	No Hit
GCTGAGTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAG	5883	0.11442871614209313	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGTAACGAGTGGAATCACTGCT	5538	0.10771821009602442	No Hit
GTACATGGGAACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAA	5499	0.10695963115168622	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0017700175367891338	0.0	0.0	0.0	0.0
2	0.0018089190211141696	0.0	0.0	0.0	0.0
3	0.0018672712476017234	0.0	0.0	0.0	0.0
4	0.0019061727319267595	0.0	0.0	0.0	0.0
5	0.0019256234740892774	0.0	0.0	0.0	0.0
6	0.0019256234740892774	1.9450742162517953E-5	0.0	0.0	0.0
7	0.0019256234740892774	1.9450742162517953E-5	0.0	0.0	0.0
8	0.0019450742162517953	1.9450742162517953E-5	0.0	0.0	0.0
9	0.0019645249584143132	1.9450742162517953E-5	0.0	0.0	0.0
10-11	0.001983975700576831	1.9450742162517953E-5	0.0	0.0	0.0
12-13	0.00209095478247068	1.9450742162517953E-5	0.0	0.0	0.0
14-15	0.002421617399233485	1.9450742162517953E-5	0.0	0.0	0.0
16-17	0.002810632242483844	1.9450742162517953E-5	0.0	0.0	0.0
18-19	0.0030634918905965776	3.8901484325035907E-5	0.0	0.0	0.0
20-21	0.003549760444659526	3.8901484325035907E-5	0.0	0.0	0.0
22-23	0.00436669161548528	3.8901484325035907E-5	0.0	0.0	0.0
24-25	0.005348954094692437	3.8901484325035907E-5	0.0	0.0	0.0
26-27	0.006457646397955961	3.8901484325035907E-5	0.0	0.0	0.0
28-29	0.007585789443382002	4.862685540629488E-5	0.0	0.0	0.0
30-31	0.009375257722333653	5.8352226487553856E-5	0.0	0.0	0.0
32-33	0.01215671385157372	5.8352226487553856E-5	0.0	0.0	0.0
34-35	0.015278557968657852	5.8352226487553856E-5	0.0	0.0	0.0
36-37	0.01842957819898576	5.8352226487553856E-5	1.9450742162517953E-5	0.0	0.0
38-39	0.022271099776083055	7.780296865007181E-5	1.9450742162517953E-5	0.0	0.0
40-41	0.028164674651325994	8.752833973133079E-5	1.9450742162517953E-5	0.0	0.0
42-43	0.03444726436981929	9.725371081258976E-5	1.9450742162517953E-5	0.0	0.0
44-45	0.04232481494563907	9.725371081258976E-5	1.9450742162517953E-5	0.0	0.0
46-47	0.048500425582238514	9.725371081258976E-5	1.9450742162517953E-5	0.0	0.0
48-49	0.05522065699938847	9.725371081258976E-5	1.9450742162517953E-5	0.0	0.0
50-51	0.06498492956497248	9.725371081258976E-5	1.9450742162517953E-5	0.0	0.0
52-53	0.07685960765518969	9.725371081258976E-5	1.9450742162517953E-5	0.0	0.0
54-55	0.09121425537112794	9.725371081258976E-5	1.9450742162517953E-5	0.0	0.0
56-57	0.10593846718815403	9.725371081258976E-5	1.9450742162517953E-5	0.0	0.0
58-59	0.12233544283115666	9.725371081258976E-5	1.9450742162517953E-5	0.0	0.0
60-61	0.1431671876872134	9.725371081258976E-5	1.9450742162517953E-5	0.0	0.0
62-63	0.17250863223937174	9.725371081258976E-5	1.9450742162517953E-5	1.9450742162517953E-5	0.0
64-65	0.20676138918756584	9.725371081258976E-5	1.9450742162517953E-5	1.9450742162517953E-5	0.0
66-67	0.24472923788880085	9.725371081258976E-5	1.9450742162517953E-5	1.9450742162517953E-5	0.0
68-69	0.28971880451070486	9.725371081258976E-5	1.9450742162517953E-5	1.9450742162517953E-5	0.0
70-71	0.34925752627017237	9.725371081258976E-5	1.9450742162517953E-5	1.9450742162517953E-5	0.0
72-73	0.4161486285670716	9.725371081258976E-5	1.9450742162517953E-5	1.9450742162517953E-5	0.0
74-75	0.494836605985538	9.725371081258976E-5	1.9450742162517953E-5	1.9450742162517953E-5	0.0
76-77	0.5857493748531468	1.0697908189384874E-4	1.9450742162517953E-5	1.9450742162517953E-5	0.0
78-79	0.6942650653778346	1.1670445297510771E-4	1.9450742162517953E-5	1.9450742162517953E-5	0.0
80-81	0.8363527368750282	2.1395816378769748E-4	1.9450742162517953E-5	1.9450742162517953E-5	0.0
82-83	1.0122652489928405	2.1395816378769748E-4	1.9450742162517953E-5	1.9450742162517953E-5	0.0
84-85	1.2259219262770191	2.1395816378769748E-4	1.9450742162517953E-5	1.9450742162517953E-5	0.0
86-87	1.4701065433852694	2.1395816378769748E-4	1.9450742162517953E-5	1.9450742162517953E-5	0.0
88	1.6736196586316947	2.1395816378769748E-4	1.9450742162517953E-5	3.8901484325035907E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	6755	0.0	86.795944	1
CGTGTGG	2300	0.0	60.07665	3
AACGTGT	2220	0.0	59.868797	1
ACGTGTG	2425	0.0	55.81815	2
ATCGAGT	1105	0.0	52.035767	1
GTGTGGA	3225	0.0	45.468548	4
TCGAGTG	1385	0.0	43.775852	2
AGTACTT	51265	0.0	38.569695	20-21
GAGTACT	50985	0.0	38.07631	20-21
GTACTTT	49960	0.0	37.717915	22-23
TACTTTT	51840	0.0	37.147865	22-23
AGCGAGT	1110	0.0	36.940323	1
TACGGCG	275	0.0	35.88855	6
ACTTTTT	53385	0.0	34.984684	24-25
GTGGAAT	337735	0.0	34.973377	6
TGGAATC	340340	0.0	34.70569	7
ATCTGCG	680	0.0	34.559578	94
AGTGGAA	337940	0.0	34.500187	5
GGAATCA	343240	0.0	34.438477	8
GAATCAC	344040	0.0	34.366596	9
>>END_MODULE