FastQCFastQC Report
Thu 26 May 2016
SRR2132638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132638_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5236127
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3165196.044906855773361No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG1197972.2878933226791482No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA593091.13268834006509No Hit
TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT266640.5092313459929448No Hit
AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG197390.37697710540634327No Hit
AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT120670.23045659511314373No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT115880.2213086122624604No Hit
ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112520.2148916556072838No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTATGGATCTCGTA90810.17342971245731817TruSeq Adapter, Index 6 (97% over 36bp)
TACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG83100.1587050887039218No Hit
GTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTT80410.15356770376272386No Hit
ACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTC78550.1500154599000368No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC76320.145756586881869No Hit
ACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACT73810.14096296747576978No Hit
CCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGAT71330.13622664232552037No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGTAACGAGTGGTATCAACGCA70120.13391577400624546No Hit
AACGAGTGGTATCAACGCAGAGTACATGGAAACGAGTGGTATCAACGCAG61740.11791157853887042No Hit
CCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATA58730.11216305486860803No Hit
ATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTA58710.11212485869804151No Hit
CACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCT58370.11147552379841054No Hit
ACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAGA57150.10914555739385237No Hit
TGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTC56840.1085535167500712No Hit
GCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCC56490.10788508376515696No Hit
ACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55730.10643362928362893No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA53990.10311056244434103No Hit
GAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAGAGT53720.10259491414169289No Hit
TTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTG52790.10081879221034937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT81750.088.289491
ATCGAGT36850.079.750041
TCGAGTG43000.076.189492
ATGAGTG57950.059.778472
TGAGTGG63750.058.0934263
AATGAGT56400.056.0245741
GACGAGT21600.055.7281461
AGTGGTA2046500.048.3916785
TGGTATC2079000.047.726517
GTGGTAT2073850.047.7231186
GGTATCA2087400.047.5592168
GTATCAA2095050.047.470799
GAGTGGT2201300.044.9929474
CGAGTGG2163550.044.333883
ACGAGTG2112550.043.8630142
ACGATTG11050.043.813272
AACGAGT2013050.042.0559041
AGTACTT536700.040.45826320-21
GAGTACT530000.040.39336420-21
GTACTTT533150.039.48058322-23
Produced by FastQC (version 0.11.5)