FastQCFastQC Report
Thu 26 May 2016
SRR2132637_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132637_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5307913
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3315736.246767797437524No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG1279692.410909899992709No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA535391.0086638571506352No Hit
TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT282130.5315271746164641No Hit
AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG228390.43028210899462743No Hit
AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127840.24084795662626723No Hit
ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118600.2234399847925164No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA113700.21420848457764852No Hit
TACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG88200.1661670038676218No Hit
GTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTT85770.1615889333529016No Hit
ACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTC82900.15618191179847898No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82050.15458052910814477No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGTAACGAGTGGTATCAACGCA77780.14653593606375992No Hit
ACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACT76600.14431284009364886No Hit
CCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGAT76590.14429400029729197No Hit
CACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCT66170.1246629324934301No Hit
CCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATA65580.12355138450837458No Hit
AACGAGTGGTATCAACGCAGAGTACATGGAAACGAGTGGTATCAACGCAG64380.12129060894554979No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC64120.12080077424027108No Hit
ATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTA63050.11878491603008565No Hit
TGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTC62090.11697629557982582No Hit
ACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTG58870.11090988115291264No Hit
TGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATG58480.11017512909499458No Hit
GCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCC57520.10836650864473475No Hit
GAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAGAGT56670.10676512595440055No Hit
CTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTT56040.10557821878391752No Hit
TCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCG56000.10550285959849003No Hit
CTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCG55680.10489998611507008No Hit
GATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGT55460.10448551059521888No Hit
TTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTG55450.104466670798862No Hit
ACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAGA55190.1039768360935833No Hit
CGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGT55020.10365655955551646No Hit
GTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCAC54270.10224357482875096No Hit
TCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGA53130.10009583804406741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT83250.088.0379941
ATCGAGT37950.082.255431
TCGAGTG44550.077.858572
ATGAGTG65550.058.65142
TGAGTGG71300.058.003493
AATGAGT60600.057.2521741
GACGAGT24450.051.915041
CGATTGT4750.048.480093
AGTGGTA2169800.048.096645
GTGGTAT2202500.047.3859376
TGGTATC2209500.047.298737
GTATCAA2219900.047.216419
GGTATCA2216500.047.191318
GAGTGGT2334900.044.6876954
TTCGAGT7400.044.4707641
CGAGTGG2292100.043.9266363
ACGAGTG2238400.043.426282
AACGAGT2135800.041.6829871
AGTACTT564700.040.69033420-21
GAGTACT557600.040.58899320-21
Produced by FastQC (version 0.11.5)