##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132637_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5307913
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.612547530451234	31.0	23.0	33.0	16.0	34.0
2	27.93138244730085	31.0	25.0	34.0	16.0	34.0
3	28.08300475158504	31.0	25.0	34.0	16.0	34.0
4	33.899447673690204	35.0	33.0	37.0	28.0	37.0
5	34.88906864147924	35.0	35.0	37.0	32.0	37.0
6	35.29724639420427	37.0	35.0	37.0	33.0	37.0
7	35.53205280493482	37.0	35.0	37.0	33.0	37.0
8	35.666431232011526	37.0	35.0	37.0	35.0	37.0
9	37.470691023006594	39.0	37.0	39.0	35.0	39.0
10-11	37.444554573520705	39.0	37.0	39.0	34.5	39.0
12-13	37.6730656662986	39.0	37.0	39.0	35.0	39.0
14-15	39.14146925543052	40.5	39.0	41.0	36.0	41.0
16-17	39.04272988272415	40.5	39.0	41.0	36.0	41.0
18-19	39.03265134526508	40.0	38.5	41.0	35.5	41.0
20-21	38.97577880421175	40.0	39.0	41.0	35.0	41.0
22-23	38.79634067099442	40.0	38.5	41.0	35.0	41.0
24-25	38.553287422005596	40.0	38.0	41.0	34.0	41.0
26-27	38.715161684074324	40.0	38.0	41.0	34.5	41.0
28-29	38.69565486472744	40.0	38.0	41.0	35.0	41.0
30-31	38.5339921735718	40.0	38.0	41.0	34.0	41.0
32-33	38.41296211900986	40.0	38.0	41.0	34.0	41.0
34-35	38.300785638347875	40.0	38.0	41.0	34.0	41.0
36-37	37.92057631690648	40.0	37.5	41.0	33.5	41.0
38-39	37.58120291346147	40.0	35.0	41.0	33.0	41.0
40-41	37.344756686856016	40.0	35.0	41.0	33.0	41.0
42-43	37.3119943751904	40.0	35.0	41.0	33.0	41.0
44-45	37.2059806745137	40.0	35.0	41.0	33.0	41.0
46-47	37.02647858018773	40.0	35.0	41.0	32.5	41.0
48-49	36.83576831421314	40.0	35.0	41.0	32.0	41.0
50-51	36.535297677260345	39.5	35.0	41.0	31.0	41.0
52-53	35.86467882951359	39.0	35.0	41.0	29.0	41.0
54-55	34.9340645749092	39.0	34.5	41.0	25.5	41.0
56-57	34.406936681140024	39.0	34.0	41.0	22.0	41.0
58-59	34.16190261596224	38.5	34.0	40.0	20.0	41.0
60-61	33.741361341076995	37.5	34.0	40.0	17.5	41.0
62-63	33.42630276720813	37.0	34.0	40.0	14.0	41.0
64-65	33.03861357561814	36.5	33.5	40.0	11.0	41.0
66-67	32.54920512073201	36.0	33.0	39.0	7.5	41.0
68-69	32.23951522943199	35.0	33.0	39.0	7.0	41.0
70-71	31.83505748869659	35.0	32.0	38.5	2.0	40.5
72-73	31.653797641370538	35.0	33.0	37.0	2.0	40.0
74-75	31.410363357500394	35.0	33.0	37.0	2.0	39.5
76-77	31.1336917164995	35.0	33.0	36.5	2.0	39.0
78-79	30.80834708481469	35.0	32.0	36.0	2.0	39.0
80-81	30.481612735551618	35.0	32.0	36.0	2.0	38.0
82-83	30.090965413336654	35.0	31.5	35.0	2.0	37.0
84-85	29.786838160308957	35.0	31.0	35.0	2.0	37.0
86-87	29.727248167029114	35.0	31.5	35.0	2.0	36.0
88-89	29.606021519192197	35.0	31.5	35.0	2.0	36.0
90-91	29.372312903395365	35.0	31.0	35.0	2.0	36.0
92-93	29.23060852730631	35.0	31.0	35.0	2.0	36.0
94-95	28.97713489275352	35.0	31.0	35.0	2.0	35.5
96-97	28.714358166759702	35.0	30.5	35.0	2.0	35.0
98-99	28.564351186615156	35.0	30.5	35.0	2.0	35.0
100	28.327947349551508	35.0	30.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.7617191053753913
1101	2	3.968125884247165
1101	3	3.3861616098975595
1101	4	1.8869379264714823
1101	5	1.0619520683327295
1101	6	0.8725911547615794
1101	7	0.48871325618542016
1101	8	0.5492229327176403
1101	9	0.6757019688040344
1101	10-11	0.47303636307520947
1101	12-13	0.4377390650334121
1101	14-15	0.536658979073863
1101	16-17	0.607023790938058
1101	18-19	0.48721836447374756
1101	20-21	0.5629165434406005
1101	22-23	0.5415771294512481
1101	24-25	0.5746215620053476
1101	26-27	0.5398667641797488
1101	28-29	0.6019595916034604
1101	30-31	0.658842662491331
1101	32-33	0.600807564307388
1101	34-35	0.5947643088604622
1101	36-37	0.5493660175387731
1101	38-39	0.5830263351250551
1101	40-41	0.5545177977361746
1101	42-43	0.5454665643881569
1101	44-45	0.5546040947030235
1101	46-47	0.604683513368677
1101	48-49	0.5883505086900911
1101	50-51	0.5388847225833757
1101	52-53	0.48502833283263413
1101	54-55	0.49667883659058987
1101	56-57	0.4934444208799249
1101	58-59	0.5031649412173778
1101	60-61	0.47542939638382364
1101	62-63	0.5058754347984902
1101	64-65	0.49407064217723473
1101	66-67	0.5556132055039811
1101	68-69	0.5882665014641475
1101	70-71	0.5924458035371991
1101	72-73	0.6120823522117753
1101	74-75	0.5785636459750734
1101	76-77	0.5701002069314001
1101	78-79	0.592647980945344
1101	80-81	0.5406822869179422
1101	82-83	0.5892896807643133
1101	84-85	0.5976217926143761
1101	86-87	0.5735950774114116
1101	88-89	0.5762921745315843
1101	90-91	0.576417843996424
1101	92-93	0.6267618767314005
1101	94-95	0.6660864512924647
1101	96-97	0.7135629953870755
1101	98-99	0.73235872568236
1101	100	0.732753135958113
1102	1	2.948060475458135
1102	2	3.5208841431232756
1102	3	4.256282241223726
1102	4	2.1362951641617656
1102	5	1.135361368852756
1102	6	0.9036748855349899
1102	7	0.4786627909788592
1102	8	0.5457547857304803
1102	9	0.6270872793489559
1102	10-11	0.4690713541100493
1102	12-13	0.3819442445825558
1102	14-15	0.4793022387843209
1102	16-17	0.5637408995138244
1102	18-19	0.46294523788787956
1102	20-21	0.49925810281519034
1102	22-23	0.41669496362634817
1102	24-25	0.4738608999964242
1102	26-27	0.42448699085819186
1102	28-29	0.4367488559017687
1102	30-31	0.5265747947242048
1102	32-33	0.5506525342842465
1102	34-35	0.5211804666960802
1102	36-37	0.5021760695765636
1102	38-39	0.5135228097828062
1102	40-41	0.5625934684627154
1102	42-43	0.5871876524609974
1102	44-45	0.541857094323575
1102	46-47	0.5202226995476948
1102	48-49	0.56044463857517
1102	50-51	0.5508198356677809
1102	52-53	0.5940387558241937
1102	54-55	0.48618250171758604
1102	56-57	0.41630340631639484
1102	58-59	0.37022716960178315
1102	60-61	0.35372040260395465
1102	62-63	0.31485606616543294
1102	64-65	0.3354247079185839
1102	66-67	0.3532550222281188
1102	68-69	0.3131881127979028
1102	70-71	0.4256522676499408
1102	72-73	0.3759833921811868
1102	74-75	0.4021419695540551
1102	76-77	0.392008481344007
1102	78-79	0.4643941778537126
1102	80-81	0.5230352340900808
1102	82-83	0.47359510053937015
1102	84-85	0.45219900053632855
1102	86-87	0.4037877532147611
1102	88-89	0.4418698160774781
1102	90-91	0.5296661212221956
1102	92-93	0.4746780064644618
1102	94-95	0.551026217170481
1102	96-97	0.589477296852106
1102	98-99	0.5980804911287905
1102	100	0.6952316185713663
1103	1	2.615727325374266
1103	2	3.2393026361976
1103	3	3.060395700405447
1103	4	1.712787632779758
1103	5	0.9062153925026166
1103	6	0.7340602946063086
1103	7	0.39691823901952006
1103	8	0.4691813712547983
1103	9	0.5214874064702641
1103	10-11	0.349263667798958
1103	12-13	0.34794198664164355
1103	14-15	0.42664313532269915
1103	16-17	0.5020959301814116
1103	18-19	0.3904122391022753
1103	20-21	0.4165361776792196
1103	22-23	0.3885215122444734
1103	24-25	0.3655658290652184
1103	26-27	0.30312446063163634
1103	28-29	0.3291617301802532
1103	30-31	0.364829849782339
1103	32-33	0.32190191117200584
1103	34-35	0.3017476088288973
1103	36-37	0.341191140029359
1103	38-39	0.3709351650680617
1103	40-41	0.3596005434296856
1103	42-43	0.39278466340172
1103	44-45	0.33835025920409123
1103	46-47	0.35782871405985617
1103	48-49	0.3866922619919748
1103	50-51	0.3818090922943469
1103	52-53	0.356578251636158
1103	54-55	0.3147805237340009
1103	56-57	0.42969123180310476
1103	58-59	0.3502159012549413
1103	60-61	0.3902767052534628
1103	62-63	0.37324419279636345
1103	64-65	0.41161069288016705
1103	66-67	0.38314734501542347
1103	68-69	0.3706584757215339
1103	70-71	0.39379849217708696
1103	72-73	0.3905883956176517
1103	74-75	0.41035237837975913
1103	76-77	0.4377006880306027
1103	78-79	0.4080253193269954
1103	80-81	0.4357301075048561
1103	82-83	0.4152848228929429
1103	84-85	0.4393894146672963
1103	86-87	0.45181852630963704
1103	88-89	0.45677374252823455
1103	90-91	0.47632474456168694
1103	92-93	0.47758618396099806
1103	94-95	0.4974067199403649
1103	96-97	0.46418887117001617
1103	98-99	0.487791418306994
1103	100	0.5336401076774209
1104	1	2.4841973811113363
1104	2	3.213852344886863
1104	3	1.5818048993636147
1104	4	1.0524535172701803
1104	5	0.5844695501185555
1104	6	0.5460877197274385
1104	7	0.322541683361635
1104	8	0.36287342429727687
1104	9	0.46155983219637875
1104	10-11	0.3754187845239656
1104	12-13	0.29217372992413004
1104	14-15	0.3359632388010141
1104	16-17	0.45089685023496173
1104	18-19	0.3479431881373358
1104	20-21	0.4053739635343021
1104	22-23	0.3550933642870504
1104	24-25	0.3712571292746034
1104	26-27	0.3708633389543934
1104	28-29	0.41917358325162013
1104	30-31	0.37236920744292235
1104	32-33	0.3473089692877167
1104	34-35	0.40045394516695154
1104	36-37	0.40725854558934316
1104	38-39	0.3891735981635023
1104	40-41	0.3419280637633122
1104	42-43	0.3760489834828107
1104	44-45	0.3569585865639908
1104	46-47	0.38334754614830757
1104	48-49	0.3715254532632315
1104	50-51	0.40610040715399265
1104	52-53	0.3777453731217193
1104	54-55	0.3481502695775305
1104	56-57	0.4407066164971951
1104	58-59	0.4099475442520628
1104	60-61	0.39282254718281706
1104	62-63	0.3384093212209791
1104	64-65	0.32653896236656266
1104	66-67	0.35743997783031745
1104	68-69	0.3714347833338536
1104	70-71	0.3060843502046744
1104	72-73	0.23587001991826995
1104	74-75	0.30193626431289644
1104	76-77	0.3614667746335485
1104	78-79	0.38682727029863173
1104	80-81	0.43961026011483995
1104	82-83	0.37888151352508004
1104	84-85	0.37006373954205785
1104	86-87	0.37809235137029873
1104	88-89	0.43096624626877755
1104	90-91	0.400561969969619
1104	92-93	0.3745237533090702
1104	94-95	0.3610697843618098
1104	96-97	0.42982653095610246
1104	98-99	0.4918422230305737
1104	100	0.44093176790529043
1105	1	1.36856779754455
1105	2	2.6273115585060864
1105	3	1.5951117450813825
1105	4	0.9783447167979276
1105	5	0.6767311563849887
1105	6	0.6367863742792181
1105	7	0.28594792123156054
1105	8	0.37196629092075284
1105	9	0.49357508147917173
1105	10-11	0.35140803649462526
1105	12-13	0.3380432049643005
1105	14-15	0.4141364810964845
1105	16-17	0.44178361946578804
1105	18-19	0.32943740146254896
1105	20-21	0.3646657960577002
1105	22-23	0.3337871260171923
1105	24-25	0.33924217676284485
1105	26-27	0.3430251207721966
1105	28-29	0.34837830202975795
1105	30-31	0.36829089510687396
1105	32-33	0.3599946591001171
1105	34-35	0.35132952075966983
1105	36-37	0.31904977174592375
1105	38-39	0.2849083384477922
1105	40-41	0.24101987091420796
1105	42-43	0.35184525655585475
1105	44-45	0.4055217886827478
1105	46-47	0.42358152297497753
1105	48-49	0.3586642587143203
1105	50-51	0.3473661763125122
1105	52-53	0.3027827835836021
1105	54-55	0.3097100051420938
1105	56-57	0.30833487878280863
1105	58-59	0.3482632326867332
1105	60-61	0.3001358376086216
1105	62-63	-0.006526859793410722
1105	64-65	0.12821354379892824
1105	66-67	0.2689600895157156
1105	68-69	0.3560537483341335
1105	70-71	0.38026623407695936
1105	72-73	0.31855587775960714
1105	74-75	0.30810318758993915
1105	76-77	0.28113767449405813
1105	78-79	0.2808777219113665
1105	80-81	0.34072460425586826
1105	82-83	0.3556004830716084
1105	84-85	0.3491904407484441
1105	86-87	0.3437435137242204
1105	88-89	0.3408818653607817
1105	90-91	0.37294613221530426
1105	92-93	0.32254273105354514
1105	94-95	0.2489194313378249
1105	96-97	0.29455926892623197
1105	98-99	0.2802837628748449
1105	100	0.2824224866809182
1106	1	1.879626469808784
1106	2	2.8636270279303204
1106	3	1.2734780805965613
1106	4	0.886365807971508
1106	5	0.6033389987757474
1106	6	0.5784059390125336
1106	7	0.2980249970761051
1106	8	0.4086512572910408
1106	9	0.40746146857419063
1106	10-11	0.2828028080205698
1106	12-13	0.22408520503352491
1106	14-15	0.21529508844653122
1106	16-17	0.312514300860137
1106	18-19	0.11933259986754763
1106	20-21	0.26386444354970706
1106	22-23	0.22916055186079376
1106	24-25	0.19821300404304054
1106	26-27	0.21664251920447697
1106	28-29	0.2783133192351812
1106	30-31	0.24887904989253684
1106	32-33	0.22398584041963687
1106	34-35	0.27291082214988904
1106	36-37	0.28549228237481117
1106	38-39	0.2958109405405196
1106	40-41	0.19609386512762939
1106	42-43	0.2846777276219328
1106	44-45	0.20312589114404034
1106	46-47	0.19659659344865332
1106	48-49	0.15849760905175003
1106	50-51	0.31081713173948344
1106	52-53	0.2411516069233599
1106	54-55	0.09705738740834136
1106	56-57	0.10894811223501222
1106	58-59	0.19268127124058765
1106	60-61	0.03460129223942232
1106	62-63	0.149661884506898
1106	64-65	-0.0026616916564066173
1106	66-67	-0.134665764571956
1106	68-69	0.08642968538944729
1106	70-71	0.20499724158722543
1106	72-73	0.25340448317319186
1106	74-75	0.2933503892195901
1106	76-77	0.3339731113327815
1106	78-79	0.266614373829583
1106	80-81	0.35029808691461994
1106	82-83	0.3044909745385773
1106	84-85	0.19610232784701864
1106	86-87	0.26302916193873926
1106	88-89	0.23082675363956895
1106	90-91	0.30652163393533627
1106	92-93	0.2791503191623228
1106	94-95	0.2918575374314649
1106	96-97	0.28085974932073654
1106	98-99	0.27771891664698245
1106	100	0.20082637598757813
1107	1	1.6753293103458375
1107	2	0.9878332765759801
1107	3	0.5732346102540511
1107	4	0.3639767429723264
1107	5	0.24894722397350932
1107	6	0.37839037967589917
1107	7	0.18946133210878457
1107	8	0.2143719124653316
1107	9	0.2777222750699124
1107	10-11	0.17327729836767247
1107	12-13	0.17234381291236645
1107	14-15	0.2183751572403665
1107	16-17	0.23834790006573314
1107	18-19	0.2085407397872956
1107	20-21	0.19422710345961747
1107	22-23	0.11491444446315313
1107	24-25	0.0935208141358288
1107	26-27	0.14278229941966458
1107	28-29	0.19680170099475447
1107	30-31	0.16441444224857804
1107	32-33	0.11298575086166096
1107	34-35	0.19727174907557554
1107	36-37	0.17776800116367042
1107	38-39	0.2337136538375333
1107	40-41	0.23884886541478068
1107	42-43	0.26031237730077095
1107	44-45	0.20469251037729919
1107	46-47	0.22396197836523868
1107	48-49	0.1651585627874539
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2216	92-93	-1.8045514521434143
2216	94-95	-1.8890347489750496
2216	96-97	-1.9414698756901636
2216	98-99	-2.0589168740097534
2216	100	-2.0855310064285426
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5396.0
3	121.0
4	284.0
5	521.0
6	985.0
7	1590.0
8	2617.0
9	3842.0
10	6452.0
11	11089.0
12	12879.0
13	16056.0
14	16273.0
15	18397.0
16	19671.0
17	25442.0
18	35993.0
19	60847.0
20	107624.0
21	113026.0
22	55490.0
23	38974.0
24	37780.0
25	40160.0
26	43516.0
27	51231.0
28	62879.0
29	77529.0
30	93944.0
31	115044.0
32	145532.0
33	193085.0
34	273822.0
35	459828.0
36	674928.0
37	1024349.0
38	1123707.0
39	335586.0
40	1424.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	16.98409279482545	42.658136893586445	24.563123317068648	15.794646994519454
2	18.252654241048255	41.697515912633655	23.053699450620574	16.996130395697516
3	19.048831137315695	22.173535572697308	20.881680325598694	37.8959529643883
4	37.317246910414696	23.4693748748331	21.234277954442735	17.979100260309465
5	17.026172328718346	42.263501833655035	22.114868855748387	18.59545698187823
6	39.05521569251018	21.485243479618987	22.364826331982947	17.09471449588789
7	17.738276908005048	21.497767478663878	42.81928823075039	17.944667382580683
8	38.36019919693484	21.76621960457905	22.3744624299607	17.499118768525406
9	37.69383559979224	22.490967730631606	22.965824797806594	16.849371871769563
10-11	16.58417347835204	33.31372424529189	32.71156667413351	17.390535602222567
12-13	16.39973187201825	22.80390616801745	32.686858281211464	28.109503678752834
14-15	16.634372115744927	43.69872867170204	21.8785989898478	17.78830022270523
16-17	27.488110675514086	22.313515688746214	22.0088667617574	28.189506873982296
18-19	17.196909595164804	32.965545968820514	22.243959160596642	27.593585275418043
20-21	27.74789262747901	32.8764808315434	22.431066598114928	16.944559942862668
22-23	27.071430899489123	22.5419953190642	33.11549190802487	17.271081873421814
24-25	16.71374772578137	32.81450910506989	22.415491948083854	28.056251221064883
26-27	17.082490068281388	28.15000239273122	37.505760453315176	17.261747085672212
28-29	29.030746921417418	22.321412254720858	31.434135392259172	17.213705431602556
30-31	24.63144419240827	25.257362638190767	32.34979878972396	17.761394379677007
32-33	19.462727189828467	26.887294929080074	32.90238514935727	20.74759273173419
34-35	20.789857153192916	26.383578797114428	33.24341347646653	19.583150573226135
36-37	20.83443197915168	24.782774288645086	35.21566570494532	19.16712802725791
38-39	23.00112969058988	24.578768222760814	33.364137938310904	19.055964148338397
40-41	18.934188474622257	26.81499122469032	34.98220915477235	19.268611145915077
42-43	18.987670903923	25.05932984757427	34.87430626401285	21.078692984489876
44-45	19.0411914395242	28.806979682567675	32.733762389631806	19.418066488276317
46-47	21.21031666401937	24.707897447608453	32.46573538281351	21.61605050555867
48-49	19.356905943054343	27.017449811124862	32.62538562890781	21.00025861691298
50-51	21.432311315308404	27.11660657152296	32.39658741899409	19.054494694174547
52-53	21.24143115744908	25.51390445290122	33.620631262208825	19.62403312744088
54-55	19.68184000283365	27.945977537302802	30.357772676539724	22.014409783323824
56-57	20.044448346507192	28.511818791220513	30.703535633096912	20.74019722917539
58-59	23.91305883799286	27.27157196023173	28.39326820219887	20.422100999576543
60-61	21.769342425871734	29.163585516141428	28.425607373318428	20.641464684668406
62-63	19.690380665424467	29.20692206655074	28.33025484529773	22.77244242272706
64-65	21.726736975188523	29.18323693350084	28.388250384256846	20.701775707053795
66-67	22.081083298782637	26.864727173860516	30.337583139524423	20.716606387832428
68-69	23.78278444904792	27.027753677048977	29.079873426426524	20.109588447476582
70-71	19.69905757548524	28.90020202750988	30.928465531069715	20.472274865935162
72-73	19.4094779807129	27.173557431033085	30.962398434966115	22.4545661532879
74-75	19.720571085140897	30.673105002270244	28.711629510587265	20.894694402001587
76-77	21.80771799913467	27.153883609512658	28.410483902646543	22.62791448870613
78-79	20.412659114761013	29.09565815194061	27.97522195219207	22.5164607811063
80-81	21.998289314906415	29.06386149479588	28.22604028211232	20.711808908185382
82-83	21.75908529433625	27.82032535021952	29.67519143110595	20.745397924338285
84-85	20.28502466108494	29.398355061030756	27.723837296656377	22.59278298122792
86-87	20.255033760618986	29.59934798894965	29.153709137094236	20.99190911333713
88-89	24.314193462573648	27.584310221977386	27.288351679158257	20.81314463629071
90-91	22.186954907648044	29.563458888008544	27.508888446569046	20.74069775777437
92-93	20.56819107754059	29.71821705298567	27.165888188308084	22.54770368116566
94-95	21.937125433470637	30.130322140520928	27.076582567577994	20.85596985843044
96-97	21.99472366201896	27.70897923226136	29.047224035051496	21.249073070668185
98-99	24.031758595037406	28.116349583442336	26.831157622323747	21.02073419919651
100	20.578884631710075	27.829176037138627	30.27822506729125	21.313714263860057
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	54012.0
1	31116.5
2	6027.0
3	3348.5
4	2625.0
5	2303.5
6	2246.5
7	2276.5
8	2131.5
9	2070.0
10	2316.0
11	4210.5
12	32454.5
13	36176.0
14	12221.5
15	11754.0
16	13599.5
17	15595.5
18	17444.0
19	19772.0
20	22323.0
21	25460.5
22	28811.5
23	33279.5
24	38481.5
25	43793.0
26	52228.0
27	61846.0
28	68424.5
29	73143.0
30	78193.5
31	83896.0
32	92263.5
33	103182.0
34	115735.0
35	125021.0
36	130675.5
37	135179.5
38	134964.0
39	132115.5
40	131045.5
41	132543.0
42	133283.5
43	133489.5
44	136366.5
45	142522.5
46	158731.0
47	190812.0
48	208550.0
49	234173.0
50	343620.0
51	329331.5
52	186463.0
53	131891.5
54	122005.0
55	118677.0
56	108896.0
57	96136.5
58	91975.0
59	79526.5
60	61017.5
61	49705.0
62	39844.0
63	30946.5
64	23568.5
65	17557.0
66	12722.0
67	9718.0
68	7227.0
69	5178.5
70	3998.5
71	2973.0
72	2152.5
73	1573.0
74	1168.0
75	882.0
76	595.0
77	372.5
78	267.5
79	204.5
80	139.5
81	91.5
82	56.0
83	35.0
84	30.5
85	29.0
86	27.5
87	24.5
88	17.0
89	11.0
90	7.0
91	5.0
92	6.0
93	5.0
94	3.5
95	3.0
96	2.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02494389037650014
2	0.012283547224681338
3	0.005180943998140135
4	0.0
5	0.0039186776422296295
6	0.0010550285959849003
7	2.4491735263935184E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	8.666306324161681E-4
26-27	0.0032216051770253204
28-29	6.02873483419943E-4
30-31	6.217132797768163E-4
32-33	0.004465031736578953
34-35	0.0077054767099611465
36-37	0.004568650616541756
38-39	0.004992546034571403
40-41	0.007686636913604273
42-43	0.009580036447470032
44-45	0.008496748156949821
46-47	0.011605314555833903
48-49	0.015740649856167573
50-51	0.058977982495191615
52-53	0.009994511967321243
54-55	0.005067905219998896
56-57	0.0033723235478803066
58-59	0.007827935386280823
60-61	0.014647941667468928
62-63	0.012321226817395087
64-65	0.011124899748733636
66-67	0.009316279298473807
68-69	0.006179453205054417
70-71	0.004144755198512108
72-73	0.0031745056861331375
74-75	0.002317294951895406
76-77	0.0018745597375088854
78-79	0.0013093658468026888
80-81	0.0018463000429735754
82-83	0.0011963270686614494
84-85	0.001064448494163337
86-87	5.180943998140135E-4
88-89	0.0014129847267654915
90-91	7.441719560964921E-4
92-93	2.920168435315349E-4
94-95	5.180943998140135E-4
96-97	0.0012245867631967592
98-99	0.003475942427843109
100	3.014367417099715E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5307913.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.42852518122017
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.47381605266777	43.764090668898326
2	10.872923174090953	11.401026528525996
3	2.7011847901305583	4.248574043654667
4	0.958414490210896	2.00993032936273
5	0.47402888311172225	1.24263176174243
6	0.2966650730647037	0.9332227352136804
7	0.1951893863352797	0.7163444159610287
8	0.14675810234440662	0.6155448691449448
9	0.10935997275602619	0.5160223876911184
>10	0.5946846526744357	5.577783022882489
>50	0.1274443462438578	4.891986810058771
>100	0.041436362153395016	4.0333861727612055
>500	0.0033668285845994284	1.2410706794343362
>1k	0.003331010331227452	3.8859456518020834
>5k	0.0011103367770756447	3.652585353859725
>10k+	2.865385231162954E-4	11.26985456900633
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	331573	6.246767797437524	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG	127969	2.410909899992709	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	53539	1.0086638571506352	No Hit
TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	28213	0.5315271746164641	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG	22839	0.43028210899462743	No Hit
AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12784	0.24084795662626723	No Hit
ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11860	0.2234399847925164	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA	11370	0.21420848457764852	No Hit
TACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG	8820	0.1661670038676218	No Hit
GTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTT	8577	0.1615889333529016	No Hit
ACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTC	8290	0.15618191179847898	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8205	0.15458052910814477	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGTAACGAGTGGTATCAACGCA	7778	0.14653593606375992	No Hit
ACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACT	7660	0.14431284009364886	No Hit
CCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGAT	7659	0.14429400029729197	No Hit
CACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCT	6617	0.1246629324934301	No Hit
CCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATA	6558	0.12355138450837458	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGAAACGAGTGGTATCAACGCAG	6438	0.12129060894554979	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGGTTAAGCGACTAAGCGTAC	6412	0.12080077424027108	No Hit
ATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTA	6305	0.11878491603008565	No Hit
TGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTC	6209	0.11697629557982582	No Hit
ACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTG	5887	0.11090988115291264	No Hit
TGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATG	5848	0.11017512909499458	No Hit
GCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCC	5752	0.10836650864473475	No Hit
GAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAGAGT	5667	0.10676512595440055	No Hit
CTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTT	5604	0.10557821878391752	No Hit
TCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCG	5600	0.10550285959849003	No Hit
CTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCG	5568	0.10489998611507008	No Hit
GATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGT	5546	0.10448551059521888	No Hit
TTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTG	5545	0.104466670798862	No Hit
ACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAGA	5519	0.1039768360935833	No Hit
CGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGT	5502	0.10365655955551646	No Hit
GTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCAC	5427	0.10224357482875096	No Hit
TCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGA	5313	0.10009583804406741	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.021741124995831695	0.0	0.0	0.0	0.0
2	0.025377205692708227	0.0	0.0	0.0	0.0
3	0.028824888426016024	0.0	0.0	0.0	3.767959271374644E-5
4	0.02914516496408287	0.0	0.0	0.0	5.651938907061966E-5
5	0.02925820374222411	0.0	0.0	0.0	9.41989817843661E-5
6	0.029352402724008474	0.0	0.0	0.0	9.41989817843661E-5
7	0.029408922113079095	0.0	0.0	0.0	9.41989817843661E-5
8	0.029521960891220334	0.0	0.0	0.0	9.41989817843661E-5
9	0.03023787315278152	0.0	0.0	0.0	1.1303877814123932E-4
10-11	0.030445110912707123	0.0	0.0	0.0	1.3187857449811253E-4
12-13	0.03047337060724243	0.0	0.0	0.0	1.6013826903342237E-4
14-15	0.030520470098134616	0.0	0.0	0.0	1.6955816721185896E-4
16-17	0.03058640938538367	0.0	0.0	0.0	1.7897806539029557E-4
18-19	0.030661768570811165	0.0	0.0	0.0	2.1665765810404203E-4
20-21	0.030803067043487713	0.0	0.0	0.0	2.4491735263935184E-4
22-23	0.030963205312521135	0.0	0.0	0.0	3.014367417099715E-4
24-25	0.031208122665160487	0.0	0.0	0.0	3.014367417099715E-4
26-27	0.03150013950869202	0.0	0.0	0.0	3.1085663988840814E-4
28-29	0.03183925584311574	0.0	0.0	0.0	3.391163344237179E-4
30-31	0.032404449733821936	0.0	0.0	0.0	3.6737602895902776E-4
32-33	0.03316746148627531	0.0	0.0	0.0	4.2389541802964744E-4
34-35	0.03386453395147961	0.0	0.0	0.0	4.615750107433939E-4
36-37	0.03435436865675831	0.0	0.0	0.0	4.8041480710026707E-4
38-39	0.03500434163107044	0.0	0.0	0.0	5.086745016355769E-4
40-41	0.03619124880155346	0.0	0.0	0.0	5.275142979924501E-4
42-43	0.03742525546292865	0.0	0.0	0.0	5.746137888846333E-4
44-45	0.039007798356906	0.0	0.0	0.0	6.311331779552528E-4
46-47	0.041023656567091435	0.0	0.0	0.0	6.782326688474358E-4
48-49	0.0432279127408456	0.0	0.0	0.0	6.782326688474358E-4
50-51	0.046948772521328064	0.0	0.0	0.0	7.064923633827457E-4
52-53	0.051037008330769545	0.0	0.0	0.0	7.347520579180555E-4
54-55	0.05548320027099163	0.0	0.0	0.0	7.441719560964922E-4
56-57	0.06131411724344389	0.0	0.0	0.0	7.72431650631802E-4
58-59	0.06809644393191824	0.0	0.0	0.0	8.572107342377316E-4
60-61	0.07747866251764111	0.0	0.0	0.0	9.325699196652244E-4
62-63	0.0906476801710955	0.0	0.0	0.0	9.419898178436609E-4
64-65	0.1070665626961105	0.0	0.0	1.883979635687322E-5	9.514097160220976E-4
66-67	0.12624547538740744	9.41989817843661E-6	0.0	1.883979635687322E-5	9.702495123789707E-4
68-69	0.15242337242528278	1.883979635687322E-5	0.0	1.883979635687322E-5	9.796694105574074E-4
70-71	0.18687194006382546	1.883979635687322E-5	0.0	1.883979635687322E-5	9.89089308735844E-4
72-73	0.23182369417132495	1.883979635687322E-5	0.0	1.883979635687322E-5	0.0010267689014495905
74-75	0.28862568018729773	1.883979635687322E-5	0.0	1.883979635687322E-5	0.001064448494163337
76-77	0.3578901914933421	1.883979635687322E-5	0.0	1.883979635687322E-5	0.00108328829052021
78-79	0.44330040827722683	1.883979635687322E-5	0.0	3.767959271374644E-5	0.0011209678832339565
80-81	0.5520248730527422	1.883979635687322E-5	0.0	3.767959271374644E-5	0.0011303877814123932
82-83	0.68802936295301	1.883979635687322E-5	0.0	3.767959271374644E-5	0.0011303877814123932
84-85	0.8532449571046097	1.883979635687322E-5	0.0	3.767959271374644E-5	0.001158647475947703
86-87	1.044581928905014	1.883979635687322E-5	0.0	3.767959271374644E-5	0.0012151668650183228
88	1.2098917220383982	1.883979635687322E-5	0.0	3.767959271374644E-5	0.0012622663559105057
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	8325	0.0	88.037994	1
ATCGAGT	3795	0.0	82.25543	1
TCGAGTG	4455	0.0	77.85857	2
ATGAGTG	6555	0.0	58.6514	2
TGAGTGG	7130	0.0	58.00349	3
AATGAGT	6060	0.0	57.252174	1
GACGAGT	2445	0.0	51.91504	1
CGATTGT	475	0.0	48.48009	3
AGTGGTA	216980	0.0	48.09664	5
GTGGTAT	220250	0.0	47.385937	6
TGGTATC	220950	0.0	47.29873	7
GTATCAA	221990	0.0	47.21641	9
GGTATCA	221650	0.0	47.19131	8
GAGTGGT	233490	0.0	44.687695	4
TTCGAGT	740	0.0	44.470764	1
CGAGTGG	229210	0.0	43.926636	3
ACGAGTG	223840	0.0	43.42628	2
AACGAGT	213580	0.0	41.682987	1
AGTACTT	56470	0.0	40.690334	20-21
GAGTACT	55760	0.0	40.588993	20-21
>>END_MODULE