FastQCFastQC Report
Thu 26 May 2016
SRR2132636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132636_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6339115
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2846324.490090493704563No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG1114171.7576112753909654No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA412500.6507217490138608No Hit
TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT238580.3763616845569137No Hit
AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG205950.32488762232582935No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111510.17590783571523785No Hit
AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT107680.16986598286984855No Hit
ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT101820.16062179026567588No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA95300.15033644286308104No Hit
GTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTT87940.13872598935340344No Hit
ACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACT83810.13221088432691314No Hit
TACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG78120.1232348679586977No Hit
CCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGAT75960.1198274522547706No Hit
ACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTC75910.11974857689125375No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGTAACGAGTGGTATCAACGCA69470.10958943007028582No Hit
ATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTA68680.10834319932671989No Hit
CACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCT66510.1049200085500894No Hit
CCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATA65340.10307432504379554No Hit
TGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTC65100.10269572329891476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT71950.086.0738141
ATCGAGT34300.074.991021
TCGAGTG41150.068.883112
ATGAGTG63700.049.6639062
GACGAGT23400.048.8322221
TGAGTGG67750.048.7673383
AGTGGTA2001100.045.305445
TGGTATC2020400.044.945677
GTGGTAT2018450.044.891316
GGTATCA2024500.044.861618
GTATCAA2030050.044.817679
AATGAGT65900.044.0980221
GAGTGGT2149700.042.160544
CGAGTGG2109000.041.618873
ACGAGTG2061650.041.228432
ACGATTG9200.040.364962
AACGAGT1967450.039.651421
GAGTACT497850.039.50945320-21
AGTACTT511250.039.2736220-21
GTACTTT503350.038.38247722-23
Produced by FastQC (version 0.11.5)