##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132636_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6339115 Sequences flagged as poor quality 0 Sequence length 100 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.50379855863161 31.0 19.0 33.0 10.0 34.0 2 27.81157432859319 31.0 25.0 34.0 16.0 34.0 3 28.01177199025416 31.0 25.0 34.0 16.0 34.0 4 33.858380231309894 35.0 33.0 37.0 28.0 37.0 5 34.91699140968416 35.0 35.0 37.0 32.0 37.0 6 35.33390402288016 37.0 35.0 37.0 33.0 37.0 7 35.61086744758535 37.0 35.0 37.0 33.0 37.0 8 35.71047977517367 37.0 35.0 37.0 35.0 37.0 9 37.50712946523292 39.0 37.0 39.0 35.0 39.0 10-11 37.536816495678025 39.0 37.0 39.0 34.5 39.0 12-13 37.689739734963 39.0 37.0 39.0 35.0 39.0 14-15 39.152932862079325 40.5 39.0 41.0 36.0 41.0 16-17 39.07846339749318 40.5 39.0 41.0 36.0 41.0 18-19 39.094389279891594 40.0 39.0 41.0 35.5 41.0 20-21 39.05271272093975 40.0 39.0 41.0 35.0 41.0 22-23 38.923211678601824 40.0 38.5 41.0 35.0 41.0 24-25 38.76327152922766 40.0 38.0 41.0 34.5 41.0 26-27 38.8405708052307 40.0 38.0 41.0 35.0 41.0 28-29 38.79836207420121 40.0 38.5 41.0 35.0 41.0 30-31 38.667037748960226 40.0 38.0 41.0 35.0 41.0 32-33 38.57719658027973 40.0 38.0 41.0 34.5 41.0 34-35 38.472794151865045 40.0 38.0 41.0 34.0 41.0 36-37 38.17026935147887 40.0 37.5 41.0 33.5 41.0 38-39 37.909097642178764 40.0 37.0 41.0 33.0 41.0 40-41 37.72413728099269 40.0 36.5 41.0 33.0 41.0 42-43 37.68841731061828 40.0 37.0 41.0 33.0 41.0 44-45 37.59647229936671 40.0 36.0 41.0 33.0 41.0 46-47 37.45285193595636 40.0 36.0 41.0 33.0 41.0 48-49 37.29565254771368 40.0 36.0 41.0 33.0 41.0 50-51 37.05184272568016 40.0 35.5 41.0 32.0 41.0 52-53 36.53206117888696 40.0 35.0 41.0 30.5 41.0 54-55 35.88117221410244 39.0 35.0 41.0 28.5 41.0 56-57 35.428951517680304 39.0 35.0 41.0 26.5 41.0 58-59 35.18245291337986 39.0 35.0 41.0 26.0 41.0 60-61 34.817596541473065 38.5 35.0 41.0 26.0 41.0 62-63 34.52687883087781 38.0 35.0 40.0 25.0 41.0 64-65 34.17984726574608 37.0 34.0 40.0 23.5 41.0 66-67 33.74140869821734 37.0 34.0 40.0 22.5 41.0 68-69 33.423143609163105 36.0 33.0 39.5 22.0 41.0 70-71 33.03845095096082 36.0 33.0 39.0 21.0 41.0 72-73 32.81002387557254 35.0 33.0 39.0 21.0 40.5 74-75 32.548867073716124 35.0 33.0 37.5 20.5 40.0 76-77 32.277963485439216 35.0 33.0 37.0 20.0 39.5 78-79 31.939940827702287 35.0 33.0 37.0 19.5 39.0 80-81 31.601786921360475 35.0 33.0 36.0 17.5 39.0 82-83 31.239191511748878 35.0 33.0 36.0 13.0 37.5 84-85 30.971368637420206 35.0 32.0 35.5 8.5 37.0 86-87 30.846787998009184 35.0 32.5 35.0 6.5 37.0 88-89 30.707265209733535 35.0 33.0 35.0 2.0 36.0 90-91 30.4873326166192 35.0 33.0 35.0 2.0 36.0 92-93 30.338333505544544 35.0 32.5 35.0 2.0 36.0 94-95 30.112293198656282 35.0 32.0 35.0 2.0 36.0 96-97 29.883187006388116 35.0 32.0 35.0 2.0 35.5 98-99 29.740159233583867 35.0 32.0 35.0 2.0 35.0 100 29.547661779286226 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 3.972758041677345 1101 2 4.07515219788063 1101 3 3.4294251994590255 1101 4 1.9182525943709052 1101 5 1.0578202601112068 1101 6 0.8418490888501466 1101 7 0.4682224448419987 1101 8 0.5150634443036424 1101 9 0.6324539670481499 1101 10-11 0.38902642949086186 1101 12-13 0.4027711389134083 1101 14-15 0.4854688616476892 1101 16-17 0.5145465854400157 1101 18-19 0.44615954538165425 1101 20-21 0.486462636240482 1101 22-23 0.4274362160706957 1101 24-25 0.48009686811299446 1101 26-27 0.4469800369848471 1101 28-29 0.42210807462452493 1101 30-31 0.4838191955396667 1101 32-33 0.43331708848295847 1101 34-35 0.43141730619149143 1101 36-37 0.3896801214308212 1101 38-39 0.3876816665129539 1101 40-41 0.383556317220318 1101 42-43 0.42484759231609814 1101 44-45 0.4489524794304742 1101 46-47 0.4685098415419162 1101 48-49 0.45694575383622293 1101 50-51 0.4069995338246457 1101 52-53 0.3645684232482296 1101 54-55 0.3349377276742942 1101 56-57 0.32967405833778685 1101 58-59 0.2651042021777741 1101 60-61 0.2790481284899613 1101 62-63 0.3241902093816904 1101 64-65 0.3248032066141775 1101 66-67 0.3081572683482605 1101 68-69 0.30889056241591817 1101 70-71 0.2528328161242541 1101 72-73 0.25938223001520555 1101 74-75 0.26533411306547805 1101 76-77 0.24822225907112738 1101 78-79 0.2560920924315617 1101 80-81 0.2642487130989011 1101 82-83 0.25687939286423855 1101 84-85 0.30756791664361316 1101 86-87 0.3439963263612036 1101 88-89 0.3176273704191921 1101 90-91 0.35209590930660895 1101 92-93 0.33476679336123993 1101 94-95 0.3603559612713312 1101 96-97 0.4014797627204736 1101 98-99 0.4149186645494751 1101 100 0.4082172519333511 1102 1 2.98219975260562 1102 2 3.6258136814811692 1102 3 4.284309714142694 1102 4 2.1841085296474674 1102 5 1.148327102550553 1102 6 0.89760441806866 1102 7 0.4842957723495189 1102 8 0.525402719367122 1102 9 0.6139120329734169 1102 10-11 0.43112003347685146 1102 12-13 0.38310819629266746 1102 14-15 0.5110865181550963 1102 16-17 0.537197732372583 1102 18-19 0.46444521878152756 1102 20-21 0.5375687843572052 1102 22-23 0.4110692699029883 1102 24-25 0.48691353655703296 1102 26-27 0.4398758951709141 1102 28-29 0.4323713094796915 1102 30-31 0.4091726852596693 1102 32-33 0.42837102985490105 1102 34-35 0.4391131442822527 1102 36-37 0.4391272687684378 1102 38-39 0.42965400964428113 1102 40-41 0.43280349432846066 1102 42-43 0.4292622281712255 1102 44-45 0.40984296220489114 1102 46-47 0.4073132301998257 1102 48-49 0.39559835527830955 1102 50-51 0.4407049820734983 1102 52-53 0.3871462094845768 1102 54-55 0.28155937398556574 1102 56-57 0.2871851280333715 1102 58-59 0.23403787678370236 1102 60-61 0.24665040629457025 1102 62-63 0.20798349389949067 1102 64-65 0.23903143450240094 1102 66-67 0.13181427308742144 1102 68-69 0.15688544109237057 1102 70-71 0.1995915106858206 1102 72-73 0.16349637565825503 1102 74-75 0.13816036145844635 1102 76-77 0.1714407466535306 1102 78-79 0.1958858039332263 1102 80-81 0.20851516138222692 1102 82-83 0.1952147876631365 1102 84-85 0.17235924969033078 1102 86-87 0.14919558957193146 1102 88-89 0.14261781004415752 1102 90-91 0.16726302995918374 1102 92-93 0.18180133076178961 1102 94-95 0.2002243039097138 1102 96-97 0.18599228208382712 1102 98-99 0.22823127314989478 1102 100 0.20757851004518457 1103 1 2.78375040801399 1103 2 3.4034408479599882 1103 3 3.128401545006607 1103 4 1.7611453626050064 1103 5 0.9256105121333462 1103 6 0.8020382810750704 1103 7 0.43400407168789457 1103 8 0.4993560272699895 1103 9 0.5863804624364022 1103 10-11 0.40288604674304196 1103 12-13 0.40379973504921907 1103 14-15 0.46776033096155345 1103 16-17 0.5282294591369805 1103 18-19 0.4650285776131895 1103 20-21 0.4883255230060968 1103 22-23 0.47130192669074944 1103 24-25 0.4557978415709911 1103 26-27 0.4159873909769729 1103 28-29 0.4615781207426508 1103 30-31 0.38470019497739116 1103 32-33 0.3862669207854026 1103 34-35 0.3875776239706141 1103 36-37 0.4246604027528562 1103 38-39 0.47855794536430807 1103 40-41 0.4470132862746965 1103 42-43 0.4620593757477707 1103 44-45 0.4084898582535317 1103 46-47 0.4529240278692015 1103 48-49 0.46010073818597164 1103 50-51 0.4836070628134195 1103 52-53 0.4414367827838248 1103 54-55 0.47080657976950846 1103 56-57 0.5041537203200477 1103 58-59 0.4377579884580527 1103 60-61 0.4286533032492059 1103 62-63 0.47901337693392776 1103 64-65 0.46346776040181936 1103 66-67 0.4918320398294398 1103 68-69 0.4940936154852622 1103 70-71 0.4869757357953759 1103 72-73 0.46589006404773414 1103 74-75 0.46572583016062197 1103 76-77 0.5069593141190509 1103 78-79 0.45150448601676274 1103 80-81 0.42717637547517384 1103 82-83 0.46018314539628236 1103 84-85 0.47058350220978795 1103 86-87 0.47421224121343997 1103 88-89 0.46006610396061376 1103 90-91 0.45055344409711395 1103 92-93 0.501534376792673 1103 94-95 0.5660685163357329 1103 96-97 0.5370393677339464 1103 98-99 0.5595511260221855 1103 100 0.5448983158934588 1104 1 2.6567806228193973 1104 2 3.384332872689658 1104 3 1.7361847182050099 1104 4 1.1889636307829647 1104 5 0.6534016704822818 1104 6 0.6116413559249239 1104 7 0.34372378561542405 1104 8 0.3943928836798136 1104 9 0.4543951361628231 1104 10-11 0.33472362546076084 1104 12-13 0.3207459292260495 1104 14-15 0.42322630215346635 1104 16-17 0.47032473712712175 1104 18-19 0.352330001658693 1104 20-21 0.36433663459411747 1104 22-23 0.3402030250133876 1104 24-25 0.3735538482506655 1104 26-27 0.376334093093611 1104 28-29 0.4120429054999164 1104 30-31 0.3916234865115129 1104 32-33 0.3268042339523163 1104 34-35 0.38768970762518506 1104 36-37 0.3471992392532499 1104 38-39 0.3773325871663289 1104 40-41 0.3615175610837369 1104 42-43 0.41259273480743275 1104 44-45 0.33237271666207135 1104 46-47 0.2935652654217549 1104 48-49 0.3482259750679759 1104 50-51 0.40238494200278296 1104 52-53 0.35029277836851236 1104 54-55 0.18824680451991327 1104 56-57 0.22611708540501496 1104 58-59 0.2271235961879441 1104 60-61 0.19209424107516782 1104 62-63 0.18936739809700498 1104 64-65 0.1935626164585571 1104 66-67 0.1464962869253057 1104 68-69 0.20354775836924688 1104 70-71 0.1554740942233579 1104 72-73 0.041396394263671255 1104 74-75 0.17223804031423384 1104 76-77 0.1330443146697462 1104 78-79 0.1907204025435938 1104 80-81 0.24861543160769983 1104 82-83 0.19788827103687723 1104 84-85 0.1818152015567236 1104 86-87 0.18537535613481992 1104 88-89 0.22669385381645668 1104 90-91 0.2763701504735714 1104 92-93 0.2706136731438029 1104 94-95 0.27535201307040325 1104 96-97 0.2647556349271234 1104 98-99 0.21852960174927105 1104 100 0.25238639890662995 1105 1 1.4096993917538612 1105 2 2.6843620775465205 1105 3 1.477052723897799 1105 4 0.9348189606241633 1105 5 0.5863799675289059 1105 6 0.5450608681827305 1105 7 0.22634540993956875 1105 8 0.3206923743165859 1105 9 0.4552272386555103 1105 10-11 0.3053693158318467 1105 12-13 0.24995204102252444 1105 14-15 0.33712367273247423 1105 16-17 0.39708408127578565 1105 18-19 0.2932394904963971 1105 20-21 0.35439042313368674 1105 22-23 0.3154149886238784 1105 24-25 0.361755557934508 1105 26-27 0.293882597853127 1105 28-29 0.2843057658772139 1105 30-31 0.2867351115103105 1105 32-33 0.2406722741809233 1105 34-35 0.2536627664710167 1105 36-37 0.2499030226927701 1105 38-39 0.21460701503625756 1105 40-41 0.17469495011933844 1105 42-43 0.2860254660528767 1105 44-45 0.2759511870704898 1105 46-47 0.2914744727523484 1105 48-49 0.23690585028121092 1105 50-51 0.2983968074672845 1105 52-53 0.14257219987742076 1105 54-55 0.18220803524812368 1105 56-57 0.21900505019293348 1105 58-59 0.1348746344713021 1105 60-61 0.15693778275588954 1105 62-63 -0.1234535792426783 1105 64-65 0.0025066465400556126 1105 66-67 0.1294117669250454 1105 68-69 0.21833563613492402 1105 70-71 0.21119640894267633 1105 72-73 0.12684097571359843 1105 74-75 0.12619581545229153 1105 76-77 0.11851025512035562 1105 78-79 0.07622126031704468 1105 80-81 0.09613611093536534 1105 82-83 0.05341433895679515 1105 84-85 0.04912534388446943 1105 86-87 0.09833947140736754 1105 88-89 0.08998442798655049 1105 90-91 0.11137711084732871 1105 92-93 0.10398504105954132 1105 94-95 0.09168281384368626 1105 96-97 0.11215778811518007 1105 98-99 0.12775434080302261 1105 100 0.1694209624380889 1106 1 1.7930859966766626 1106 2 2.882340458693516 1106 3 1.1666465768060377 1106 4 0.8128321177215483 1106 5 0.5158601165247063 1106 6 0.5265328343152618 1106 7 0.26902357776541663 1106 8 0.3501056503711837 1106 9 0.3983008933112302 1106 10-11 0.2711520417495805 1106 12-13 0.20373352440707038 1106 14-15 0.19514955949439639 1106 16-17 0.2961133849148112 1106 18-19 0.10378563358369064 1106 20-21 0.21155982447003652 1106 22-23 0.24475291262517374 1106 24-25 0.27198714171466065 1106 26-27 0.20260847796668457 1106 28-29 0.2798386736143996 1106 30-31 0.2927179379351017 1106 32-33 0.25110076475840515 1106 34-35 0.24644764539372233 1106 36-37 0.3040781740440224 1106 38-39 0.3191168713873793 1106 40-41 0.23854755313393383 1106 42-43 0.29218696903538444 1106 44-45 0.27277963359105684 1106 46-47 0.18196981606143225 1106 48-49 0.13124309622102714 1106 50-51 0.29670351124707395 1106 52-53 0.22236002057353232 1106 54-55 0.16132010072742986 1106 56-57 0.25550242676516177 1106 58-59 0.3201794475547288 1106 60-61 0.17125896742069813 1106 62-63 0.2855607036180672 1106 64-65 0.11246781708074138 1106 66-67 0.027326585937309744 1106 68-69 0.30557150830106394 1106 70-71 0.40563093434169417 1106 72-73 0.4383304311664773 1106 74-75 0.495094892946895 1106 76-77 0.44536993645493794 1106 78-79 0.42705360271172665 1106 80-81 0.42929628076844395 1106 82-83 0.39044405100787216 1106 84-85 0.3067472201244428 1106 86-87 0.4141460669321084 1106 88-89 0.34857021254262577 1106 90-91 0.3869152944764842 1106 92-93 0.4193035452490612 1106 94-95 0.39922969021219856 1106 96-97 0.35663417262891883 1106 98-99 0.39391239151191826 1106 100 0.4221285497811884 1107 1 1.6966151311323152 1107 2 0.891374608401378 1107 3 0.5002288865411657 1107 4 0.40191618267193974 1107 5 0.2506325727824503 1107 6 0.3375043651456764 1107 7 0.17190552527566183 1107 8 0.1937636499504407 1107 9 0.28241329164041673 1107 10-11 0.19340803502036152 1107 12-13 0.13674488505447613 1107 14-15 0.20534435321390276 1107 16-17 0.2268499952773979 1107 18-19 0.17667894187107436 1107 20-21 0.1972286611446421 1107 22-23 0.14391631718400077 1107 24-25 0.12997986256564786 1107 26-27 0.12384502230516858 1107 28-29 0.15925282626262316 1107 30-31 0.14501948160176426 1107 32-33 0.11506624455504522 1107 34-35 0.12209097373884248 1107 36-37 0.09324365558521919 1107 38-39 0.14622149819368957 1107 40-41 0.19554411787436976 1107 42-43 0.1495527927162783 1107 44-45 0.16579089483552423 1107 46-47 0.10757529893557916 1107 48-49 0.07350702855254099 1107 50-51 0.11161108383534923 1107 52-53 0.18533067661822145 1107 54-55 0.18115450881505524 1107 56-57 0.20679641691674533 1107 58-59 0.20058040385485754 1107 60-61 0.20199610573661886 1107 62-63 0.16418125458955757 1107 64-65 0.12824212435881321 1107 66-67 0.1271828449053416 1107 68-69 0.1623368025375882 1107 70-71 0.15662364378529503 1107 72-73 0.12939223922138865 1107 74-75 0.23922598561436814 1107 76-77 0.18204401772207746 1107 78-79 0.17409509548189916 1107 80-81 0.1446781340904124 1107 82-83 0.15676525156455412 1107 84-85 0.14421763664897824 1107 86-87 0.17043175798673715 1107 88-89 0.18135550237767717 1107 90-91 0.21143298990012838 1107 92-93 0.22071715475408027 1107 94-95 0.19048479904638427 1107 96-97 0.20407477316394917 1107 98-99 0.22403181146067652 1107 100 0.2503485752983998 1108 1 1.492646951856866 1108 2 -0.43596591612698177 1108 3 0.326609784222196 1108 4 0.2557260637482557 1108 5 0.15479482846441783 1108 6 0.13601634981976218 1108 7 0.12451635611787282 1108 8 0.025693190240502872 1108 9 0.10314952984666803 1108 10-11 0.10525961520570348 1108 12-13 0.17029503225518283 1108 14-15 0.1546652417514025 1108 16-17 0.17800352383544293 1108 18-19 0.15693577893575394 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-0.30819523394909965 2215 14-15 -0.5147247574520151 2215 16-17 -0.48592454974807 2215 18-19 -0.5568589217601883 2215 20-21 -0.4846577233366176 2215 22-23 -0.48815365889311835 2215 24-25 -0.5262492966406214 2215 26-27 -0.6461886419784619 2215 28-29 -0.7216181585824586 2215 30-31 -0.702308583505804 2215 32-33 -0.7325251151726846 2215 34-35 -0.7980591191484194 2215 36-37 -0.8018486683623536 2215 38-39 -0.9163159803564653 2215 40-41 -0.9189327864856764 2215 42-43 -0.9654894831478913 2215 44-45 -1.0463658700748084 2215 46-47 -0.9505877970736663 2215 48-49 -0.9568152566107102 2215 50-51 -1.0218163473268618 2215 52-53 -1.0785787870917716 2215 54-55 -1.23520232866872 2215 56-57 -1.2113613956456817 2215 58-59 -1.1985737364422064 2215 60-61 -1.2220648084024077 2215 62-63 -1.1918797766222724 2215 64-65 -1.3066772493302707 2215 66-67 -1.2209758043093828 2215 68-69 -1.1651389858273333 2215 70-71 -1.205294950455059 2215 72-73 -1.249644368385745 2215 74-75 -1.2146003214018322 2215 76-77 -1.2228399121853748 2215 78-79 -1.2578457266628682 2215 80-81 -1.2799815808021933 2215 82-83 -1.3236591542668386 2215 84-85 -1.2726918833110084 2215 86-87 -1.3164866181778798 2215 88-89 -1.2509827539914653 2215 90-91 -1.236433591700564 2215 92-93 -1.3177073077065664 2215 94-95 -1.4404045339596863 2215 96-97 -1.5010734385288664 2215 98-99 -1.5546550546791735 2215 100 -1.5652250728436101 2216 1 -3.316500723860706 2216 2 -4.408560302372695 2216 3 -6.051673536402145 2216 4 -3.371566267888312 2216 5 -1.6787091213297742 2216 6 -0.881617907823184 2216 7 -0.6248082356326705 2216 8 -0.4183698868650083 2216 9 -0.3172290935857802 2216 10-11 -0.4867752091951374 2216 12-13 -0.419391085419889 2216 14-15 -0.6363363868212559 2216 16-17 -0.5685391799440751 2216 18-19 -0.5959595777808531 2216 20-21 -0.5601994902759131 2216 22-23 -0.5763621441548707 2216 24-25 -0.5800351763846194 2216 26-27 -0.7247157731091036 2216 28-29 -0.7131751230870904 2216 30-31 -0.8620758531135309 2216 32-33 -0.9988748391306643 2216 34-35 -1.0170919109552514 2216 36-37 -1.0385258535596478 2216 38-39 -1.1127894669809066 2216 40-41 -1.21396642749486 2216 42-43 -1.2300229484199008 2216 44-45 -1.2142274825986163 2216 46-47 -1.2862731452450262 2216 48-49 -1.329426458559638 2216 50-51 -1.347734442976197 2216 52-53 -1.3588136004452736 2216 54-55 -1.3390950068944747 2216 56-57 -1.3791938717001173 2216 58-59 -1.259587185178674 2216 60-61 -1.332001859746427 2216 62-63 -1.3418109489881616 2216 64-65 -1.371878741177305 2216 66-67 -1.3693947528024424 2216 68-69 -1.1999195689381565 2216 70-71 -1.2558132581850003 2216 72-73 -1.3296553397587942 2216 74-75 -1.3752782378246593 2216 76-77 -1.3363898216599601 2216 78-79 -1.4251631220502325 2216 80-81 -1.4151978430291798 2216 82-83 -1.3856127707161683 2216 84-85 -1.3645857768858072 2216 86-87 -1.3959368580110159 2216 88-89 -1.4287429867654247 2216 90-91 -1.4688752515402435 2216 92-93 -1.4990942273550267 2216 94-95 -1.605031982365496 2216 96-97 -1.6107663778370416 2216 98-99 -1.6978080601948875 2216 100 -1.7526568141101393 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4256.0 3 142.0 4 237.0 5 531.0 6 1016.0 7 1628.0 8 2662.0 9 3989.0 10 6393.0 11 10888.0 12 12456.0 13 15605.0 14 16201.0 15 17902.0 16 18575.0 17 23834.0 18 33508.0 19 56156.0 20 99426.0 21 104524.0 22 52300.0 23 37005.0 24 36448.0 25 39376.0 26 44713.0 27 53746.0 28 67024.0 29 82738.0 30 103253.0 31 128967.0 32 164165.0 33 216620.0 34 305046.0 35 495370.0 36 758380.0 37 1245011.0 38 1565381.0 39 511151.0 40 2492.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.54297822007651 38.8240059481844 26.660334938829767 16.972680892909327 2 19.383335113510345 37.06257540443281 25.11411145001965 18.439978032037192 3 20.58643032888007 23.997700483567357 21.71962369713526 33.69624549041731 4 32.04040311620786 25.311009502115045 23.267285733103122 19.381301648573974 5 17.87687375963501 36.99209321549932 24.204920127593923 20.926112897271747 6 34.47451743231921 22.644259976125795 24.618401564017624 18.262821027537367 7 19.297599940937932 22.664942340340748 38.49969727535197 19.53776044336935 8 33.72076386057044 22.737953168541665 24.702880449400272 18.83840252148762 9 33.28341574494231 24.226078876941024 24.81332173339654 17.677183644720124 10-11 17.747169754768606 31.83683369050727 31.905904846338963 18.51009170838516 12-13 17.54568263866486 24.331937502316965 31.888347190420113 26.23403266859806 14-15 17.803534089537735 39.36685956951404 23.960797366824867 18.86880897412336 16-17 25.74048585646419 23.919932040986794 24.00966065452354 26.329921448025473 18-19 18.350227121609247 31.62552659164568 24.21077705641876 25.813469230326312 20-21 25.93858133193671 31.579755218196865 24.348343577928464 18.133319871937957 22-23 25.298357893806944 24.16682770386718 32.04499366236454 18.489820739961335 24-25 17.91432835022716 31.548237145572962 24.31265150630185 26.22478299789803 26-27 18.25247814142371 28.14952846116901 35.20151490639464 18.396478491012637 28-29 26.845371099753507 23.903607003581993 30.874968183426894 18.37605371323761 30-31 23.463052089792942 26.105031445449516 31.600093578351284 18.83182288640626 32-33 19.902876130190002 27.261431064572427 31.796144782638702 21.03954802259887 34-35 20.954957608511222 26.90009166857232 32.034620287727776 20.11033043518868 36-37 21.103614955815804 25.734306387402672 33.474795328141504 19.68728332864002 38-39 22.60799671230759 25.583153258290125 32.269592355326346 19.539257674075937 40-41 19.59292176408457 27.173635756245286 33.44120178241674 19.792240697253412 42-43 19.53145041361821 25.935890676180655 33.339888559576195 21.192770350624933 44-45 19.56598220852682 28.671601450301974 31.70554837374273 20.05686796742847 46-47 21.25128286845047 25.686611704808914 31.518032732570934 21.54407269416968 48-49 19.996448462753154 27.364728596525094 31.64455975414085 20.9942631865809 50-51 21.490966867280182 27.392142152372724 31.441042624150427 19.675848356196664 52-53 21.180028300030813 26.36600342446465 32.349366388846335 20.1046018866582 54-55 20.194792256458012 28.061000326089374 29.931794155727477 21.81241326172514 56-57 20.512634440492807 28.51906114407881 30.152074567304172 20.816229848124205 58-59 23.38907912557909 27.564468151065025 28.404977759266842 20.641474964089046 60-61 21.711210709976527 28.978649703918425 28.61035916728757 20.69978041881748 62-63 20.32696020284757 29.05151817375974 28.236496043808206 22.385025579584482 64-65 21.74207863527045 28.95797664657546 28.381500902116425 20.91844381603767 66-67 22.015093087026 27.313868150207043 29.806433067851213 20.864605694915745 68-69 23.3005360376378 27.419110819289784 28.88848940498213 20.391863738090287 70-71 20.254224527163505 28.85545956278632 30.093658222147866 20.796657687902314 72-73 19.988575333700588 27.748569313743864 30.091209601136846 22.171645751418698 74-75 20.178928150618816 30.206284334250572 28.481642677224013 21.133144837906606 76-77 21.79753358457182 27.59575371308742 28.180933040353228 22.425779661987537 78-79 20.795221223567616 29.00909816070396 28.038594557509878 22.157086058218542 80-81 21.931918184958192 29.04665408477718 28.16963954361611 20.85178818664852 82-83 21.627981615809414 28.146814494670203 29.30767758207935 20.91752630744103 84-85 20.56071979887833 29.270662623893788 27.907405977411276 22.261211599816598 86-87 20.578300416204222 29.46641824979053 28.92783024906392 21.02745108494133 88-89 23.40915135703556 28.06664039559315 27.592893432725912 20.931314814645376 90-91 21.94677128785656 29.425072487087988 27.772658722271125 20.855497502784324 92-93 20.819703927138338 29.438831161896246 27.34855988205862 22.39290502890679 94-95 21.735168807043053 29.75202837260722 27.34352914748405 21.169273672865682 96-97 22.02406469816036 28.019423772051162 28.63743567338664 21.319075856401838 98-99 23.413144231563322 28.457852842919635 27.18457575980108 20.944427165715958 100 20.767812102122335 28.316277555357594 29.685430257246175 21.230480085273896 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 45162.0 1 26470.5 2 5680.0 3 3033.5 4 2304.0 5 2041.5 6 1971.0 7 2000.0 8 1916.5 9 1796.5 10 1904.5 11 2977.0 12 17763.5 13 20387.0 14 9028.0 15 9928.0 16 12127.5 17 14530.0 18 17179.5 19 20046.0 20 23329.0 21 27153.5 22 31600.0 23 37529.0 24 43985.5 25 50976.5 26 61219.0 27 72498.0 28 81357.5 29 88707.5 30 96579.0 31 106093.0 32 116808.0 33 129023.5 34 143695.0 35 156227.0 36 165902.0 37 172754.0 38 175413.0 39 177196.5 40 179877.5 41 182618.0 42 184341.5 43 186144.5 44 189586.0 45 195143.5 46 212265.5 47 244727.5 48 258352.5 49 279163.5 50 380300.0 51 362942.0 52 220362.0 53 159761.5 54 142643.0 55 133651.5 56 122290.0 57 107207.0 58 98069.0 59 82833.0 60 63005.5 61 50326.5 62 39601.0 63 29604.5 64 21640.0 65 15797.0 66 11424.5 67 8418.0 68 6211.0 69 4638.0 70 3422.0 71 2537.0 72 1938.0 73 1367.0 74 969.0 75 669.5 76 461.5 77 317.5 78 227.0 79 161.0 80 110.5 81 64.0 82 40.0 83 35.0 84 30.0 85 22.0 86 12.0 87 12.0 88 13.5 89 13.0 90 11.0 91 8.0 92 6.5 93 6.0 94 3.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02711734997708671 2 0.01252540772647286 3 0.006168053427016232 4 0.0 5 0.0040226435393584115 6 8.83404071388514E-4 7 3.3127652677069274E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010017171166637614 26-27 0.0038096800578629663 28-29 7.019907352998013E-4 30-31 6.625530535413855E-4 32-33 0.004771959492768312 34-35 0.008195150269398804 36-37 0.0048902725380435596 38-39 0.005087460946835639 40-41 0.008360788532784151 42-43 0.010427323056925139 44-45 0.009039116659028902 46-47 0.01230455670862573 48-49 0.016003811257565136 50-51 0.05862017016570925 52-53 0.009559694058239991 54-55 0.005710576318618608 56-57 0.003959543248544947 58-59 0.008203037805750487 60-61 0.014394753841821768 62-63 0.01198116771820672 64-65 0.010837474947212662 66-67 0.009212642458765932 68-69 0.006010302699982569 70-71 0.004251382093557224 72-73 0.003139239467969898 74-75 0.002303160614691483 76-77 0.001861458578997226 78-79 0.0013645437888411868 80-81 0.0017273704610186122 82-83 0.0012856684253243552 84-85 0.0010490423347738604 86-87 6.231153717829697E-4 88-89 0.0012146805981592067 90-91 8.676289986851477E-4 92-93 5.284649355627718E-4 94-95 6.783281262447518E-4 96-97 0.0012383432072142562 98-99 0.003715029621642769 100 1.8930087244039585E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6339115.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.43903038513312 #Duplication Level Percentage of deduplicated Percentage of total 1 86.11713549243686 53.770704396928906 2 9.965016883986726 12.444119840152236 3 2.133441919649896 3.9963013453780984 4 0.6390876328312336 1.596160485004488 5 0.27844192639119 0.8692821951217254 6 0.17927264871978818 0.671616621638289 7 0.11588797214158159 0.5065152829673771 8 0.08606990366089265 0.4299297063942365 9 0.060134651544818486 0.3379274401505683 >10 0.3114020532661132 3.8317562812073938 >50 0.08466073927653539 3.5475535144485524 >100 0.024240026591678954 2.943232381789442 >500 0.0021490037576829997 0.9423328155072507 >1k 0.00209842228795401 2.98493526258739 >5k 7.584658871487803E-4 3.0159704562723286 >10k+ 2.0225756990634142E-4 8.111661974451751 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 284632 4.490090493704563 No Hit AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG 111417 1.7576112753909654 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 41250 0.6507217490138608 No Hit TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 23858 0.3763616845569137 No Hit AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG 20595 0.32488762232582935 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11151 0.17590783571523785 No Hit AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10768 0.16986598286984855 No Hit ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10182 0.16062179026567588 No Hit AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA 9530 0.15033644286308104 No Hit GTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTT 8794 0.13872598935340344 No Hit ACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACT 8381 0.13221088432691314 No Hit TACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG 7812 0.1232348679586977 No Hit CCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGAT 7596 0.1198274522547706 No Hit ACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTC 7591 0.11974857689125375 No Hit AACGAGTGGTATCAACGCAGAGTACATGGGTAACGAGTGGTATCAACGCA 6947 0.10958943007028582 No Hit ATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTA 6868 0.10834319932671989 No Hit CACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCT 6651 0.1049200085500894 No Hit CCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATA 6534 0.10307432504379554 No Hit TGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTC 6510 0.10269572329891476 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 6.941031989481181E-4 3.155014540673264E-5 0.0 0.0 1.577507270336632E-5 2 6.941031989481181E-4 3.155014540673264E-5 0.0 0.0 1.577507270336632E-5 3 6.941031989481181E-4 3.155014540673264E-5 0.0 1.577507270336632E-5 1.577507270336632E-5 4 6.941031989481181E-4 4.7325218110098963E-5 0.0 3.155014540673264E-5 1.577507270336632E-5 5 6.941031989481181E-4 4.7325218110098963E-5 0.0 7.88753635168316E-5 1.577507270336632E-5 6 6.941031989481181E-4 6.310029081346528E-5 0.0 7.88753635168316E-5 1.577507270336632E-5 7 6.941031989481181E-4 6.310029081346528E-5 0.0 1.1042550892356425E-4 1.577507270336632E-5 8 6.941031989481181E-4 6.310029081346528E-5 0.0 1.7352579973702953E-4 1.577507270336632E-5 9 7.098782716514845E-4 6.310029081346528E-5 0.0 2.3662609055049482E-4 1.577507270336632E-5 10-11 7.098782716514845E-4 9.465043622019793E-5 0.0 2.3662609055049482E-4 1.577507270336632E-5 12-13 7.098782716514845E-4 1.0253797257188109E-4 0.0 2.602886996055443E-4 2.3662609055049482E-5 14-15 7.335408807065339E-4 1.2620058162693057E-4 0.0 2.9183884501227694E-4 3.155014540673264E-5 16-17 7.414284170582171E-4 1.2620058162693057E-4 0.0 2.997263813639601E-4 3.155014540673264E-5 18-19 7.887536351683161E-4 1.2620058162693057E-4 0.0 3.233889904190096E-4 3.155014540673264E-5 20-21 9.30729289498613E-4 1.2620058162693057E-4 0.0 3.628266721774254E-4 3.155014540673264E-5 22-23 0.0011121426255873256 1.2620058162693057E-4 0.0 3.628266721774254E-4 3.155014540673264E-5 24-25 0.0013172185707310878 1.3408811797861373E-4 0.0 3.628266721774254E-4 3.155014540673264E-5 26-27 0.0014828568341164342 1.4986319068198005E-4 0.0 3.628266721774254E-4 3.94376817584158E-5 28-29 0.0017115953883152458 1.7352579973702953E-4 0.0 3.786017448807917E-4 4.7325218110098963E-5 30-31 0.0018930087244039585 1.8930087244039585E-4 0.0 3.8648928123247487E-4 6.310029081346528E-5 32-33 0.0021059722058994037 2.0507594514376217E-4 0.0 4.1015189028752435E-4 7.88753635168316E-5 34-35 0.0025476742415936606 2.2873855419881166E-4 0.0 4.180394266392075E-4 9.465043622019793E-5 36-37 0.003036701495398017 2.3662609055049482E-4 0.0 4.3381449934257383E-4 1.0253797257188109E-4 38-39 0.0036203791854225705 2.3662609055049482E-4 0.0 4.811397174526728E-4 1.2620058162693057E-4 40-41 0.004598433693031282 2.602886996055443E-4 0.0 4.969147901560391E-4 1.419756543302969E-4 42-43 0.005592263273343361 2.997263813639601E-4 0.0 5.205773992110886E-4 1.4986319068198005E-4 44-45 0.00668863082622732 3.3127652677069274E-4 0.0 5.521275446178212E-4 1.577507270336632E-4 46-47 0.008147825051288705 3.628266721774254E-4 0.0 5.836776900245539E-4 1.7352579973702953E-4 48-49 0.009741107394328704 3.786017448807917E-4 0.0 5.836776900245539E-4 2.3662609055049482E-4 50-51 0.011965392645503354 3.786017448807917E-4 0.0 5.836776900245539E-4 2.5240116325386114E-4 52-53 0.014560392105207116 4.022643539358412E-4 0.0 5.836776900245539E-4 2.6817623595722746E-4 54-55 0.01764441881871523 4.1015189028752435E-4 0.0 5.836776900245539E-4 2.760637723089106E-4 56-57 0.021540861776446714 4.1015189028752435E-4 1.577507270336632E-5 6.073402990796034E-4 2.839513086605938E-4 58-59 0.02664409779598572 4.3381449934257383E-4 1.577507270336632E-5 6.152278354312865E-4 3.233889904190096E-4 60-61 0.032804263686650265 4.7325218110098963E-4 1.577507270336632E-5 6.152278354312865E-4 3.549391358257422E-4 62-63 0.04140956584633659 5.048023265077223E-4 1.577507270336632E-5 6.467779808380192E-4 3.8648928123247487E-4 64-65 0.05334340834643322 5.205773992110886E-4 1.577507270336632E-5 6.625530535413855E-4 4.2592696299089067E-4 66-67 0.06791957552434369 5.284649355627718E-4 1.577507270336632E-5 6.783281262447518E-4 4.2592696299089067E-4 68-69 0.08718093929515397 5.521275446178212E-4 1.577507270336632E-5 7.177658080031676E-4 4.7325218110098963E-4 70-71 0.11359629853694088 5.521275446178212E-4 1.577507270336632E-5 7.256533443548508E-4 4.7325218110098963E-4 72-73 0.14606928569682046 5.91565226376237E-4 1.577507270336632E-5 7.256533443548508E-4 4.811397174526728E-4 74-75 0.19036568984787308 6.152278354312865E-4 2.3662609055049482E-5 7.256533443548508E-4 5.048023265077223E-4 76-77 0.24579140779115066 6.231153717829697E-4 3.155014540673264E-5 7.414284170582171E-4 5.284649355627718E-4 78-79 0.31493355145000523 6.38890444486336E-4 3.155014540673264E-5 7.572034897615834E-4 5.521275446178212E-4 80-81 0.40525373021312916 6.625530535413855E-4 3.155014540673264E-5 7.572034897615834E-4 5.600150809695044E-4 82-83 0.5182426884509904 7.256533443548508E-4 3.155014540673264E-5 7.572034897615834E-4 5.994527627279202E-4 84-85 0.6574813676672533 7.256533443548508E-4 3.155014540673264E-5 7.729785624649497E-4 6.073402990796034E-4 86-87 0.8246813632502329 7.493159534099003E-4 3.155014540673264E-5 7.887536351683161E-4 6.310029081346528E-4 88 0.9711765759100442 7.729785624649497E-4 3.155014540673264E-5 7.887536351683161E-4 6.310029081346528E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 7195 0.0 86.073814 1 ATCGAGT 3430 0.0 74.99102 1 TCGAGTG 4115 0.0 68.88311 2 ATGAGTG 6370 0.0 49.663906 2 GACGAGT 2340 0.0 48.832222 1 TGAGTGG 6775 0.0 48.767338 3 AGTGGTA 200110 0.0 45.30544 5 TGGTATC 202040 0.0 44.94567 7 GTGGTAT 201845 0.0 44.89131 6 GGTATCA 202450 0.0 44.86161 8 GTATCAA 203005 0.0 44.81767 9 AATGAGT 6590 0.0 44.098022 1 GAGTGGT 214970 0.0 42.16054 4 CGAGTGG 210900 0.0 41.61887 3 ACGAGTG 206165 0.0 41.22843 2 ACGATTG 920 0.0 40.36496 2 AACGAGT 196745 0.0 39.65142 1 GAGTACT 49785 0.0 39.509453 20-21 AGTACTT 51125 0.0 39.27362 20-21 GTACTTT 50335 0.0 38.382477 22-23 >>END_MODULE