FastQCFastQC Report
Thu 26 May 2016
SRR2132635_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132635_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4964033
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2492555.0212196413682175No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA414500.8350065360161788No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG254070.5118217384936804No Hit
TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT210520.42409065370838595No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA126830.2554978985836718No Hit
AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT90430.18217042473327635No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84610.17044608688137247No Hit
ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT83720.1686531898559095No Hit
ACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50530.10179223224341981No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT54150.086.391981
ATCGAGT22200.073.48951
TCGAGTG25200.072.3727342
AGTGGTA946450.068.187625
ACGAGTG905750.067.075822
TGGTATC964000.067.037677
GTGGTAT962050.067.017916
GGTATCA967650.066.833368
CGAGTGG937050.066.7491463
GTATCAA974550.066.5192959
AACGAGT836500.066.446761
GAGTGGT974400.066.289584
GACGAGT15050.050.2965551
TGAGTGG53150.045.5406953
ATGAGTG51050.043.9204182
ACGATTG6400.043.332712
GAGTACT434400.041.1358420-21
AGTACTT442950.041.03672820-21
GTACTTT438050.040.1388822-23
AATGAGT52250.038.332941
Produced by FastQC (version 0.11.5)