##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132635_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4964033
Sequences flagged as poor quality	0
Sequence length	100
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.57864824830939	31.0	22.0	33.0	16.0	34.0
2	27.93449197456987	31.0	25.0	34.0	16.0	34.0
3	28.13699687330846	31.0	26.0	34.0	16.0	34.0
4	33.90477581434289	35.0	33.0	37.0	28.0	37.0
5	34.93986663666418	35.0	35.0	37.0	32.0	37.0
6	35.34218305962108	37.0	35.0	37.0	33.0	37.0
7	35.63600080821381	37.0	35.0	37.0	33.0	37.0
8	35.72362311048295	37.0	35.0	37.0	35.0	37.0
9	37.508116686573196	39.0	37.0	39.0	35.0	39.0
10-11	37.56729115217405	39.0	37.0	39.0	35.0	39.0
12-13	37.69455138593962	39.0	37.0	39.0	35.0	39.0
14-15	39.162403029955684	41.0	39.0	41.0	36.0	41.0
16-17	39.08825545680297	40.5	39.0	41.0	36.0	41.0
18-19	39.09611348272665	40.0	39.0	41.0	35.5	41.0
20-21	39.05797795864774	40.0	39.0	41.0	35.0	41.0
22-23	38.94264441835902	40.0	38.5	41.0	35.0	41.0
24-25	38.84418123328351	40.0	38.5	41.0	35.0	41.0
26-27	38.902020494223144	40.0	39.0	41.0	35.0	41.0
28-29	38.85222580107747	40.0	39.0	41.0	35.0	41.0
30-31	38.76491443550033	40.0	38.5	41.0	35.0	41.0
32-33	38.67633343694532	40.0	38.0	41.0	35.0	41.0
34-35	38.60488054773205	40.0	38.0	41.0	35.0	41.0
36-37	38.34393395450836	40.0	37.5	41.0	34.0	41.0
38-39	38.05536899130203	40.0	37.0	41.0	33.5	41.0
40-41	37.92126402060583	40.0	37.0	41.0	33.0	41.0
42-43	37.797824772720084	40.0	37.0	41.0	33.0	41.0
44-45	37.689448579411135	40.0	36.0	41.0	33.0	41.0
46-47	37.559770754948644	40.0	36.0	41.0	33.0	41.0
48-49	37.41930261140488	40.0	36.0	41.0	33.0	41.0
50-51	37.19080886448579	40.0	35.5	41.0	32.5	41.0
52-53	36.729653590135285	40.0	35.0	41.0	31.0	41.0
54-55	36.06358922271468	40.0	35.0	41.0	29.0	41.0
56-57	35.51974332160967	40.0	35.0	41.0	26.5	41.0
58-59	35.24958023042957	39.0	35.0	41.0	26.0	41.0
60-61	34.94461126668578	39.0	35.0	41.0	25.0	41.0
62-63	34.67163705398413	39.0	35.0	41.0	23.5	41.0
64-65	34.401710464052115	38.0	35.0	40.5	23.0	41.0
66-67	34.0825997732086	37.5	34.0	40.0	22.5	41.0
68-69	33.75480773395342	37.0	34.0	40.0	21.5	41.0
70-71	33.42160275727417	36.5	34.0	39.5	20.0	41.0
72-73	33.12326912411743	36.0	34.0	39.0	20.0	41.0
74-75	32.83241257662872	36.0	34.0	39.0	20.0	40.5
76-77	32.56497378240636	35.0	34.0	37.5	19.5	40.0
78-79	32.23510581013463	35.0	33.5	37.0	18.0	39.0
80-81	31.901387138240217	35.0	33.0	37.0	15.5	39.0
82-83	31.56461721749231	35.0	33.0	36.0	10.0	39.0
84-85	31.25150991139664	35.0	33.0	36.0	7.0	37.0
86-87	31.061413068768882	35.0	33.0	36.0	2.0	37.0
88-89	30.887327602374924	35.0	33.0	35.0	2.0	37.0
90-91	30.688153261672515	35.0	33.0	35.0	2.0	36.0
92-93	30.52577037662723	35.0	33.0	35.0	2.0	36.0
94-95	30.349022055252252	35.0	33.0	35.0	2.0	36.0
96-97	30.186627486158933	35.0	33.0	35.0	2.0	36.0
98-99	30.035868919485427	35.0	33.0	35.0	2.0	35.5
100	29.901744811124342	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	4.154928322566661
1101	2	4.160450114518465
1101	3	3.5231734309065637
1101	4	1.9699683415704996
1101	5	1.1184036617177497
1101	6	0.8602236550526925
1101	7	0.5400984342299537
1101	8	0.5316136684956945
1101	9	0.6369258682854664
1101	10-11	0.4409952137591304
1101	12-13	0.44324975134096434
1101	14-15	0.5276935649751024
1101	16-17	0.5641890642132594
1101	18-19	0.5183108978343114
1101	20-21	0.546080426412928
1101	22-23	0.530795889351225
1101	24-25	0.5542238582515964
1101	26-27	0.5252852407100832
1101	28-29	0.49902306654681894
1101	30-31	0.5029688576516236
1101	32-33	0.42797138693918413
1101	34-35	0.4811045729549548
1101	36-37	0.42098259808655314
1101	38-39	0.4410283217763933
1101	40-41	0.48204146032746564
1101	42-43	0.5145891366239468
1101	44-45	0.5502425164350484
1101	46-47	0.48221460940153804
1101	48-49	0.5436860743593002
1101	50-51	0.5528166602589337
1101	52-53	0.5001896723416479
1101	54-55	0.4264550421101134
1101	56-57	0.39522476602215306
1101	58-59	0.32686830091191865
1101	60-61	0.39462505124180325
1101	62-63	0.4067071586626554
1101	64-65	0.3892033128698742
1101	66-67	0.3751115081453591
1101	68-69	0.4257183009098924
1101	70-71	0.4307449027423971
1101	72-73	0.36090162328780906
1101	74-75	0.34145020995402575
1101	76-77	0.3063556970513446
1101	78-79	0.3620949034338139
1101	80-81	0.2860526987436991
1101	82-83	0.3265131645087962
1101	84-85	0.318465779963482
1101	86-87	0.27030463695693285
1101	88-89	0.29512329127143744
1101	90-91	0.3022595365734482
1101	92-93	0.31578573295299606
1101	94-95	0.334381141160101
1101	96-97	0.35589608870916933
1101	98-99	0.29731078643044384
1101	100	0.3366401337537823
1102	1	3.0262109435129894
1102	2	3.6049222834410877
1102	3	4.335154010804899
1102	4	2.224521808386747
1102	5	1.1568344073451655
1102	6	0.936968371442525
1102	7	0.5140293179890776
1102	8	0.5664697711660622
1102	9	0.6451862601625109
1102	10-11	0.4622218942602032
1102	12-13	0.39869943809763697
1102	14-15	0.5659049070650752
1102	16-17	0.5985648551403528
1102	18-19	0.49936527278688914
1102	20-21	0.5111670729283873
1102	22-23	0.4324688467539488
1102	24-25	0.4947070180563671
1102	26-27	0.4324177162561327
1102	28-29	0.43508540361946046
1102	30-31	0.41152700733542247
1102	32-33	0.4535592937254762
1102	34-35	0.3903899846250809
1102	36-37	0.4120197109196013
1102	38-39	0.4217920844145837
1102	40-41	0.3678152700925992
1102	42-43	0.4032818981171076
1102	44-45	0.4232856867922763
1102	46-47	0.3759792659864232
1102	48-49	0.4209260522813949
1102	50-51	0.46194733256361786
1102	52-53	0.4385745043798721
1102	54-55	0.3951308831932039
1102	56-57	0.3679458245338125
1102	58-59	0.37319434502256144
1102	60-61	0.3585330462586285
1102	62-63	0.35876260623544454
1102	64-65	0.3959580039905859
1102	66-67	0.37566338340634076
1102	68-69	0.378557334613447
1102	70-71	0.386612930204258
1102	72-73	0.35370884905293565
1102	74-75	0.35194504145361094
1102	76-77	0.3479851111122798
1102	78-79	0.3410884445458038
1102	80-81	0.3637581472141953
1102	82-83	0.37366985219735227
1102	84-85	0.3512614180132374
1102	86-87	0.3031088756601079
1102	88-89	0.35380306635710923
1102	90-91	0.33923659078305235
1102	92-93	0.40170567419055203
1102	94-95	0.4501708114568004
1102	96-97	0.44075162669017587
1102	98-99	0.46737217974787626
1102	100	0.5205627759440112
1103	1	2.7103246692813876
1103	2	3.276210680419446
1103	3	3.078520255561532
1103	4	1.7265041028114467
1103	5	0.9113691010732694
1103	6	0.7433410169351262
1103	7	0.3847197484654643
1103	8	0.4668710471940827
1103	9	0.5588312470154904
1103	10-11	0.3449934850158627
1103	12-13	0.380089475650756
1103	14-15	0.45861843465552
1103	16-17	0.5158960720582471
1103	18-19	0.4191783481837632
1103	20-21	0.42052028279334763
1103	22-23	0.4045580222558627
1103	24-25	0.4102138707570617
1103	26-27	0.36373149191228293
1103	28-29	0.3947869123807166
1103	30-31	0.40721975484618156
1103	32-33	0.3860597628534279
1103	34-35	0.33725160446667957
1103	36-37	0.3461208217230407
1103	38-39	0.3720233587720472
1103	40-41	0.36639638689172926
1103	42-43	0.38964726640544
1103	44-45	0.33217431696154875
1103	46-47	0.344796617312916
1103	48-49	0.3608381422807341
1103	50-51	0.37519158689045895
1103	52-53	0.3190809794897689
1103	54-55	0.29388855229094446
1103	56-57	0.3515285775596908
1103	58-59	0.2904228972937162
1103	60-61	0.30907488903898184
1103	62-63	0.30492459611600964
1103	64-65	0.30260088434464905
1103	66-67	0.35011650608215206
1103	68-69	0.40496388890299073
1103	70-71	0.4176474317043173
1103	72-73	0.3681501805982492
1103	74-75	0.3843049438292283
1103	76-77	0.41287159931199113
1103	78-79	0.36224251074089864
1103	80-81	0.35409288144749596
1103	82-83	0.3601525024727614
1103	84-85	0.39036323250860505
1103	86-87	0.3453041884997994
1103	88-89	0.36211873111140847
1103	90-91	0.32534854416317316
1103	92-93	0.32574919667779767
1103	94-95	0.3192414346646757
1103	96-97	0.32075533394518274
1103	98-99	0.309150512215421
1103	100	0.36534532941887576
1104	1	2.4324943900818177
1104	2	3.3236472847352907
1104	3	1.6761425729103436
1104	4	1.1184951398769059
1104	5	0.5901785351701534
1104	6	0.5297843741412933
1104	7	0.28601789659203547
1104	8	0.39853023624794304
1104	9	0.4428844023721865
1104	10-11	0.3249533856180733
1104	12-13	0.2764659994646763
1104	14-15	0.39937369064675465
1104	16-17	0.4294546014257037
1104	18-19	0.29492397946172844
1104	20-21	0.3372864147801238
1104	22-23	0.29912131987296675
1104	24-25	0.364156160498716
1104	26-27	0.28301693142974926
1104	28-29	0.3574857799902489
1104	30-31	0.2913246895490005
1104	32-33	0.22249643735792546
1104	34-35	0.21989677022173026
1104	36-37	0.23160588936235627
1104	38-39	0.20298468801183134
1104	40-41	0.19548610323393234
1104	42-43	0.20587549162187457
1104	44-45	0.17380809610005343
1104	46-47	0.11763111333780785
1104	48-49	0.1280507865434828
1104	50-51	0.17506729762302342
1104	52-53	0.17257510511679186
1104	54-55	0.1511249691201968
1104	56-57	0.2386380401551378
1104	58-59	0.2513502476326366
1104	60-61	0.26091683694276924
1104	62-63	0.3014962991576198
1104	64-65	0.23551471731106943
1104	66-67	0.17834412927584964
1104	68-69	0.26213855015000576
1104	70-71	0.24663780588982576
1104	72-73	0.13805799524989482
1104	74-75	0.2223599637930036
1104	76-77	0.27230941717864
1104	78-79	0.29015432991313617
1104	80-81	0.33144078318639103
1104	82-83	0.3144924578823911
1104	84-85	0.3086962059149876
1104	86-87	0.3440134142389688
1104	88-89	0.3772982445330655
1104	90-91	0.41396074814426953
1104	92-93	0.3357380824117726
1104	94-95	0.3702673105144534
1104	96-97	0.40138147034497607
1104	98-99	0.42709728429373683
1104	100	0.42363061598187457
1105	1	1.3058583653403346
1105	2	2.609474820985941
1105	3	1.3993390962696104
1105	4	0.8627115844116346
1105	5	0.5888839336697487
1105	6	0.5557404104475125
1105	7	0.22666426340838086
1105	8	0.3613992147889604
1105	9	0.4494362841202886
1105	10-11	0.30928093340118323
1105	12-13	0.2602748041065084
1105	14-15	0.36128992148852035
1105	16-17	0.3908090549189609
1105	18-19	0.3293436060599646
1105	20-21	0.36647359191184137
1105	22-23	0.31440448037839275
1105	24-25	0.3799352826122302
1105	26-27	0.32756675372724686
1105	28-29	0.34310864869060964
1105	30-31	0.31913028114443875
1105	32-33	0.26202653956787003
1105	34-35	0.2586025951393083
1105	36-37	0.28312725323018384
1105	38-39	0.23368305471324646
1105	40-41	0.19116611412372464
1105	42-43	0.2980130373195564
1105	44-45	0.30500659702720867
1105	46-47	0.3106260164221126
1105	48-49	0.33190075229270377
1105	50-51	0.3259271773286798
1105	52-53	0.20178536898886534
1105	54-55	0.22546309581893098
1105	56-57	0.24811921286388383
1105	58-59	0.208081663122762
1105	60-61	0.21434166004144117
1105	62-63	-0.14841443429308043
1105	64-65	-0.03683057678500745
1105	66-67	0.10308848450242891
1105	68-69	0.19538194329827263
1105	70-71	0.12844527136991957
1105	72-73	0.136138809130955
1105	74-75	0.08566642625341814
1105	76-77	0.0499485945791136
1105	78-79	0.07759150683774863
1105	80-81	0.047353014855357145
1105	82-83	0.08708313852179472
1105	84-85	0.09317538060696506
1105	86-87	0.16478760442304363
1105	88-89	0.10310145490236522
1105	90-91	0.0946042451414435
1105	92-93	0.09912360660263886
1105	94-95	0.08029922515342136
1105	96-97	0.08483498771124331
1105	98-99	0.08649498502880348
1105	100	0.11743172373556376
1106	1	1.9632018693965136
1106	2	2.943413554488785
1106	3	1.146887225583086
1106	4	0.8661018253609001
1106	5	0.5474745620751236
1106	6	0.5557404104475125
1106	7	0.3214699299539703
1106	8	0.4132214692991951
1106	9	0.41153823350883556
1106	10-11	0.23402969233083581
1106	12-13	0.21674653191859505
1106	14-15	0.2056415379069776
1106	16-17	0.29164450715289547
1106	18-19	0.07386473452588405
1106	20-21	0.21112790841505102
1106	22-23	0.2266214557991475
1106	24-25	0.29675329932127426
1106	26-27	0.23403242753763465
1106	28-29	0.23516579846661756
1106	30-31	0.24678496088773727
1106	32-33	0.23345165156690229
1106	34-35	0.26998411832613556
1106	36-37	0.23711684034726233
1106	38-39	0.28653448951164506
1106	40-41	0.21283944019225487
1106	42-43	0.23002659828335226
1106	44-45	0.12483950658042176
1106	46-47	0.11435528146630958
1106	48-49	0.12564091453994308
1106	50-51	0.19970124198541583
1106	52-53	0.20366210951435448
1106	54-55	0.2244944555477062
1106	56-57	0.22608264669364075
1106	58-59	0.2571190768532361
1106	60-61	0.07570502122318601
1106	62-63	0.2167629479565818
1106	64-65	-0.011585389716358918
1106	66-67	-0.10305027321641802
1106	68-69	0.05196264314086818
1106	70-71	0.1374051938786991
1106	72-73	0.12996372740192896
1106	74-75	0.15080748449292258
1106	76-77	0.09910708834349435
1106	78-79	0.08752006961775294
1106	80-81	0.11830591472216767
1106	82-83	0.10905916467507737
1106	84-85	0.10261962325135698
1106	86-87	0.11308642994671558
1106	88-89	0.13210012302199203
1106	90-91	0.1601690834995999
1106	92-93	0.16135874402848316
1106	94-95	0.16124122781718242
1106	96-97	0.1846654756637811
1106	98-99	0.2574599928990082
1106	100	0.2527992016384566
1107	1	1.827072199188276
1107	2	1.0296116469598502
1107	3	0.6625942742588329
1107	4	0.45810464546494956
1107	5	0.32106956053764435
1107	6	0.4232661813221128
1107	7	0.23583736636978614
1107	8	0.26742191341291743
1107	9	0.31242984964332976
1107	10-11	0.24063938600164647
1107	12-13	0.19588865960613333
1107	14-15	0.222739465981185
1107	16-17	0.312540619990088
1107	18-19	0.24997028776969898
1107	20-21	0.2635353357123833
1107	22-23	0.19283471790934925
1107	24-25	0.21442474849218485
1107	26-27	0.262338913733565
1107	28-29	0.25907032300739985
1107	30-31	0.20958024420328059
1107	32-33	0.15779551126295388
1107	34-35	0.18198471162278906
1107	36-37	0.1846904423983844
1107	38-39	0.20857078937463314
1107	40-41	0.22097529136193117
1107	42-43	0.22683913235440656
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2216	78-79	-1.0462754075227174
2216	80-81	-0.8816991401327599
2216	82-83	-0.9437305476841651
2216	84-85	-0.9862504611830474
2216	86-87	-1.0010469917372085
2216	88-89	-1.0070947077592045
2216	90-91	-1.1036861851845643
2216	92-93	-1.1683888348438103
2216	94-95	-1.1836507259888904
2216	96-97	-1.1526824175436339
2216	98-99	-1.1897210889132595
2216	100	-1.2789224074584382
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3628.0
3	101.0
4	238.0
5	429.0
6	760.0
7	1228.0
8	1827.0
9	2796.0
10	4314.0
11	7265.0
12	7614.0
13	9887.0
14	10632.0
15	12506.0
16	14017.0
17	19291.0
18	27601.0
19	47492.0
20	85238.0
21	91419.0
22	44172.0
23	29265.0
24	27277.0
25	28825.0
26	32762.0
27	39116.0
28	49177.0
29	61367.0
30	76005.0
31	93736.0
32	118236.0
33	154194.0
34	214750.0
35	341449.0
36	509325.0
37	926900.0
38	1348160.0
39	517895.0
40	3139.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.141561193507748	38.787692062169185	27.33428630612753	16.736460438195536
2	19.11340156751515	37.14639389111933	26.085239605052752	17.654964936312773
3	20.303191958718607	25.379055422614915	22.544505343300454	31.773247275366025
4	30.53726677481798	26.518135556310767	24.03402636525583	18.910571303615427
5	17.70100428358143	36.99583608406159	24.989055845306765	20.314103787050218
6	32.874019792900974	24.019248984992352	25.44187129658691	17.66485992551977
7	19.08659000558822	23.940283004842453	38.03504583186107	18.938081157708258
8	32.02956144731512	23.956488605132158	25.581941135363124	18.432008812189604
9	31.7314772081491	25.376100440911653	25.400979405253754	17.491442945685495
10-11	17.530644941320897	32.520734652650376	32.00810711774076	17.94051328828797
12-13	17.411719462783587	25.563548832169324	31.937338047510966	25.087393657536122
14-15	17.62287841358025	39.37784055827187	24.75598973657105	18.24329129157683
16-17	24.78699275367428	25.249419171870933	24.845241762091426	25.118346312363354
18-19	17.99886302125711	32.28894731360569	24.9280776336499	24.7841120314873
20-21	24.93353690436788	32.21203404570437	25.05664245181287	17.79778659811488
22-23	24.60175023010524	25.3126943354325	32.11298152127514	17.97257391318712
24-25	17.74082604038189	32.15009319633984	25.07305374263507	25.036027020643203
26-27	18.054025882768656	28.140686560795736	35.89338945651585	17.91189809991976
28-29	24.217560339025457	25.10963452204707	32.738351967861355	17.934453171066114
30-31	22.40664351797992	26.15645478396288	33.10685251288956	18.33004918516764
32-33	19.64419037273625	27.425803490808814	33.67281937266332	19.257186763791612
34-35	19.723628011926827	27.079680070916268	33.88163832702071	19.31505359013619
36-37	19.61451066719664	27.033077083159995	34.36512658022563	18.987285669417737
38-39	20.076065528326232	26.78840468585573	33.897440169436535	19.238089616381497
40-41	19.413054212946147	27.42270568920538	33.99854702027342	19.165693077575057
42-43	19.52223622891835	27.05733394929666	33.8288171308184	19.591612690966596
44-45	19.525005575643092	27.947228527629303	33.36753934127428	19.16022655545332
46-47	19.98861064472555	27.031142824993275	33.2301414361286	19.750105094152577
48-49	19.526368450702407	27.806574140414135	33.22956300002982	19.437494408853638
50-51	20.167433285607363	27.785977353984443	32.82873015933808	19.217859201070116
52-53	20.017366724992268	27.605023859099852	32.79571029804483	19.581899117863045
54-55	20.11947435634762	28.492873220873843	31.252353314827136	20.135299107951397
56-57	20.373052550232618	28.99309736843404	30.366451802403045	20.267398278930298
58-59	21.151009927779196	29.227490746379015	29.5342037705515	20.08729555529029
60-61	20.784005420634617	29.498918703879195	29.481611518566492	20.235464356919696
62-63	20.18971883674439	29.692463680372168	29.492368886433134	20.625448596450312
64-65	20.749728088934386	29.613036580487538	29.536144956375864	20.10109037420221
66-67	20.7384666898422	28.9714345636612	29.983609574442262	20.30648917205434
68-69	21.12882525670435	29.09389511185679	29.694229275512363	20.0830503559265
70-71	20.05799154563606	29.534288765502907	30.073721344438475	20.33399834442256
72-73	19.93431760231448	29.225796235893363	30.01533878591515	20.82454737587701
74-75	20.22328493381197	29.841860067831384	29.455462324029174	20.47939267432747
76-77	20.67103087071179	29.08618337694952	29.44092142851315	20.80186432382554
78-79	20.46669711273378	29.70129346093821	29.078314147409056	20.753695278918947
80-81	20.646716958615006	29.562692928553457	29.239301209581324	20.551288903250214
82-83	20.620541310412175	29.45861404349037	29.368132075671248	20.552712570426205
84-85	20.53950012731709	29.778631437974013	28.92538533564975	20.756483099059142
86-87	20.501715448138793	29.994697833948326	29.02924833536764	20.474338382545238
88-89	21.47604557996261	29.54152006469809	28.480194166797023	20.502240188542277
90-91	21.031571001741238	30.127962290100037	28.415946288493267	20.424520419665466
92-93	20.685190286708007	30.176615839271477	28.35480701386829	20.783386860152227
94-95	20.857997955080464	30.332979822057148	28.32682935034673	20.482192872515657
96-97	20.740943982734816	29.684435579243544	28.742177267689616	20.832443170332024
98-99	21.296854311389882	29.95153466835017	27.949220943590745	20.8023900766692
100	20.82520065857794	29.874785179538637	28.502955965809225	20.797058196074197
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	43834.0
1	25196.5
2	4767.5
3	2540.0
4	1941.5
5	1681.5
6	1554.0
7	1567.5
8	1548.0
9	1475.0
10	1571.0
11	2629.0
12	17138.0
13	19455.5
14	8007.5
15	8767.5
16	10646.5
17	12806.0
18	15302.0
19	18030.5
20	21013.5
21	24619.5
22	29223.0
23	35111.5
24	41466.0
25	48214.5
26	58608.5
27	69820.5
28	77161.5
29	83306.0
30	90573.0
31	98917.5
32	108360.0
33	119191.5
34	131553.0
35	141023.0
36	147794.0
37	153736.5
38	157212.0
39	159794.0
40	164048.0
41	167899.5
42	169602.5
43	171028.5
44	172035.0
45	172907.0
46	174739.5
47	177666.5
48	176971.5
49	176240.0
50	190455.0
51	178222.5
52	139595.0
53	120002.5
54	108857.5
55	99122.5
56	87059.5
57	74597.5
58	70062.5
59	57721.0
60	39067.5
61	28591.0
62	20254.5
63	14006.0
64	9856.5
65	7350.0
66	5558.0
67	3996.5
68	3010.0
69	2241.5
70	1630.0
71	1228.5
72	930.0
73	644.0
74	438.5
75	312.0
76	225.0
77	158.0
78	112.5
79	86.5
80	68.0
81	44.5
82	29.5
83	27.5
84	24.5
85	18.0
86	9.5
87	7.0
88	6.0
89	6.0
90	7.5
91	4.5
92	2.0
93	1.0
94	2.0
95	1.5
96	0.0
97	0.0
98	1.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026530846994772192
2	0.01281216301342074
3	0.00497579286841969
4	0.0
5	0.003948402438098216
6	6.244922223522688E-4
7	3.827532975707454E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0010173179751222445
26-27	0.003827532975707454
28-29	8.057964159384114E-4
30-31	7.352892295438003E-4
32-33	0.005136952151607373
34-35	0.008722746202533303
36-37	0.005318256345193515
38-39	0.005771516829158871
40-41	0.008863760575322524
42-43	0.010757382152777792
44-45	0.009669556991260937
46-47	0.012620786364635369
48-49	0.016700130720323576
50-51	0.06188516474406999
52-53	0.010435063586402428
54-55	0.0058420240155534825
56-57	0.004059199445289747
58-59	0.008773108478529454
60-61	0.01564252292440441
62-63	0.012802090558221511
64-65	0.011150207905547768
66-67	0.009357310880084803
68-69	0.0059931108435419345
70-71	0.004149851542082818
72-73	0.002941156918175201
74-75	0.0021454329574360203
76-77	0.0017828245702637352
78-79	0.0012892742655014583
80-81	0.0016921724734706638
82-83	0.0012187670791068473
84-85	9.87100609524554E-4
86-87	4.834778495630468E-4
88-89	0.0010777527063176252
90-91	6.34564677551499E-4
92-93	2.1152155918383297E-4
94-95	4.734053943638167E-4
96-97	0.0010978976167160856
98-99	0.00353543177492978
100	8.057964159384114E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4964033.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.85332409172163
#Duplication Level	Percentage of deduplicated	Percentage of total
1	87.77221187281422	61.3118076219895
2	9.20509530502212	12.860130112737906
3	1.757244714288972	3.6824815360707706
4	0.46407898146085724	1.2966983798456535
5	0.1964242910224558	0.6860444830139126
6	0.10967833118340896	0.4596837608396307
7	0.08119636174533312	0.39702850404457973
8	0.05090860233065724	0.28449080781279806
9	0.040688772179211324	0.2558021390935808
>10	0.23117311354078243	3.4779406978705145
>50	0.07110657636632645	3.3539236748656442
>100	0.0170523802497058	1.9639271359320045
>500	0.0012966192834143028	0.6211103592907776
>1k	0.0015847549717805066	1.684920510365645
>5k	1.152549070382754E-4	0.6225186824627325
>10k+	1.4406863379784425E-4	7.041491593764413
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	249255	5.0212196413682175	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	41450	0.8350065360161788	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG	25407	0.5118217384936804	No Hit
TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	21052	0.42409065370838595	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA	12683	0.2554978985836718	No Hit
AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9043	0.18217042473327635	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8461	0.17044608688137247	No Hit
ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8372	0.1686531898559095	No Hit
ACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5053	0.10179223224341981	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.252167743445702E-4	0.0	0.0	2.0144910398460285E-5	0.0
2	7.252167743445702E-4	0.0	0.0	2.0144910398460285E-5	0.0
3	7.453616847430306E-4	0.0	0.0	2.0144910398460285E-5	0.0
4	7.453616847430306E-4	0.0	0.0	2.0144910398460285E-5	0.0
5	7.453616847430306E-4	0.0	0.0	2.0144910398460285E-5	0.0
6	7.453616847430306E-4	0.0	0.0	2.0144910398460285E-5	0.0
7	7.453616847430306E-4	0.0	0.0	2.0144910398460285E-5	0.0
8	7.453616847430306E-4	0.0	0.0	2.0144910398460285E-5	0.0
9	7.453616847430306E-4	0.0	0.0	2.0144910398460285E-5	0.0
10-11	7.453616847430306E-4	0.0	0.0	2.0144910398460285E-5	0.0
12-13	7.655065951414908E-4	0.0	0.0	2.0144910398460285E-5	1.0072455199230143E-5
14-15	7.755790503407209E-4	0.0	0.0	3.0217365597690426E-5	2.0144910398460285E-5
16-17	8.158688711376416E-4	0.0	0.0	4.028982079692057E-5	2.0144910398460285E-5
18-19	8.56158691934562E-4	0.0	0.0	4.028982079692057E-5	2.0144910398460285E-5
20-21	8.964485127314827E-4	0.0	0.0	4.028982079692057E-5	3.0217365597690426E-5
22-23	9.468107887276333E-4	0.0	0.0	4.028982079692057E-5	4.028982079692057E-5
24-25	0.0010273904303214744	0.0	0.0	4.028982079692057E-5	4.028982079692057E-5
26-27	0.001057607795919165	0.0	0.0	4.028982079692057E-5	4.028982079692057E-5
28-29	0.001138187437513006	0.0	0.0	4.028982079692057E-5	4.028982079692057E-5
30-31	0.0012086946239076172	0.0	0.0	4.028982079692057E-5	6.043473119538085E-5
32-33	0.001269129355102998	0.0	0.0	4.028982079692057E-5	6.043473119538085E-5
34-35	0.0015914479214783625	0.0	0.0	4.028982079692057E-5	6.043473119538085E-5
36-37	0.0019036940326544968	0.0	0.0	4.028982079692057E-5	8.057964159384114E-5
38-39	0.0022260125990298616	0.0	0.0	4.028982079692057E-5	1.0072455199230142E-4
40-41	0.0028807221869798206	0.0	0.0	4.028982079692057E-5	1.0072455199230142E-4
42-43	0.003757025789312843	0.0	0.0	4.028982079692057E-5	1.0072455199230142E-4
44-45	0.004854923406028928	0.0	0.0	4.028982079692057E-5	1.0072455199230142E-4
46-47	0.006214704857924998	0.0	0.0	4.028982079692057E-5	1.0072455199230142E-4
48-49	0.00784644260020028	0.0	0.0	4.028982079692057E-5	1.0072455199230142E-4
50-51	0.009961658192038609	0.0	0.0	4.028982079692057E-5	1.0072455199230142E-4
52-53	0.013104264214198415	0.0	0.0	4.028982079692057E-5	1.0072455199230142E-4
54-55	0.01715339120428893	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.0072455199230142E-4
56-57	0.022642879287869358	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.0072455199230142E-4
58-59	0.029341061995357402	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.0072455199230142E-4
60-61	0.03846670640585992	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.0072455199230142E-4
62-63	0.05003995742977535	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.0072455199230142E-4
64-65	0.06461480010306137	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.1079700719153157E-4
66-67	0.0845784063079355	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.208694623907617E-4
68-69	0.11128048504109461	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.3094191758999184E-4
70-71	0.1464534985968063	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.4101437278922198E-4
72-73	0.19149751824776345	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.4101437278922198E-4
74-75	0.2477521805354638	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.4101437278922198E-4
76-77	0.3206868286330893	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.4101437278922198E-4
78-79	0.41160080926134057	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.4101437278922198E-4
80-81	0.5228712218472359	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.4101437278922198E-4
82-83	0.6620221904246003	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.4101437278922198E-4
84-85	0.8294868305670007	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.4101437278922198E-4
86-87	1.0245399255000924	2.0144910398460285E-5	0.0	4.028982079692057E-5	1.4101437278922198E-4
88	1.1950968093886563	2.0144910398460285E-5	0.0	6.043473119538085E-5	1.4101437278922198E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	5415	0.0	86.39198	1
ATCGAGT	2220	0.0	73.4895	1
TCGAGTG	2520	0.0	72.372734	2
AGTGGTA	94645	0.0	68.18762	5
ACGAGTG	90575	0.0	67.07582	2
TGGTATC	96400	0.0	67.03767	7
GTGGTAT	96205	0.0	67.01791	6
GGTATCA	96765	0.0	66.83336	8
CGAGTGG	93705	0.0	66.749146	3
GTATCAA	97455	0.0	66.519295	9
AACGAGT	83650	0.0	66.44676	1
GAGTGGT	97440	0.0	66.28958	4
GACGAGT	1505	0.0	50.296555	1
TGAGTGG	5315	0.0	45.540695	3
ATGAGTG	5105	0.0	43.920418	2
ACGATTG	640	0.0	43.33271	2
GAGTACT	43440	0.0	41.13584	20-21
AGTACTT	44295	0.0	41.036728	20-21
GTACTTT	43805	0.0	40.13888	22-23
AATGAGT	5225	0.0	38.33294	1
>>END_MODULE