FastQCFastQC Report
Thu 26 May 2016
SRR2132634_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132634_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5072248
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2678515.280715769418214No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG457130.901237478924532No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA279390.5508208589169931No Hit
TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT223890.44140191883362173No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA101620.20034509353643593No Hit
AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT100310.19776241224798158No Hit
ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT93360.18406040083213596No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57670.11369712206500944No Hit
AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG56210.11081871391146489No Hit
ACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT54160.10677711342189894No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT59600.086.6165851
ATCGAGT29400.079.799131
TCGAGTG34800.073.3487552
AGTGGTA1201550.060.644415
TGGTATC1227100.059.510167
GTGGTAT1224750.059.5021446
GGTATCA1235050.059.1727568
GTATCAA1245700.058.802679
GAGTGGT1253450.058.1446274
ACGAGTG1171800.057.999852
CGAGTGG1215100.057.9377443
AACGAGT1095150.056.89651
GACGAGT15400.053.427181
ATGAGTG54400.049.9460452
TGAGTGG57250.049.9135973
AATGAGT56250.044.132281
GAGTACT471250.040.4978320-21
AGTACTT484050.039.9948820-21
GTACTTT480950.038.90931722-23
CATGGGG236650.037.73108724-25