##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132634_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5072248 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.49231484738128 31.0 16.0 33.0 10.0 34.0 2 27.809356719150955 31.0 25.0 34.0 16.0 34.0 3 28.007080686906477 31.0 25.0 34.0 16.0 34.0 4 33.85883872397407 35.0 33.0 37.0 28.0 37.0 5 34.9228304688572 35.0 35.0 37.0 32.0 37.0 6 35.34258596977119 37.0 35.0 37.0 33.0 37.0 7 35.61583542445086 37.0 35.0 37.0 33.0 37.0 8 35.71931104315089 37.0 35.0 37.0 35.0 37.0 9 37.52046055319062 39.0 37.0 39.0 35.0 39.0 10-11 37.550499206663396 39.0 37.0 39.0 35.0 39.0 12-13 37.70645214902741 39.0 37.0 39.0 35.0 39.0 14-15 39.17261370106509 40.5 39.0 41.0 36.0 41.0 16-17 39.09672151282824 40.5 39.0 41.0 36.0 41.0 18-19 39.11974858090535 40.0 39.0 41.0 36.0 41.0 20-21 39.086303646824845 40.0 39.0 41.0 35.5 41.0 22-23 38.95948049070156 40.0 38.5 41.0 35.0 41.0 24-25 38.85028995033366 40.0 38.5 41.0 35.0 41.0 26-27 38.917720505779684 40.0 39.0 41.0 35.0 41.0 28-29 38.87033835884996 40.0 39.0 41.0 35.0 41.0 30-31 38.76410094695685 40.0 38.5 41.0 35.0 41.0 32-33 38.65681212748272 40.0 38.0 41.0 35.0 41.0 34-35 38.56643050576392 40.0 38.0 41.0 34.5 41.0 36-37 38.27441718149428 40.0 37.5 41.0 34.0 41.0 38-39 37.968474333273925 40.0 37.0 41.0 33.5 41.0 40-41 37.81106464037248 40.0 36.0 41.0 33.0 41.0 42-43 37.68961848868588 40.0 36.0 41.0 33.0 41.0 44-45 37.56880016513388 40.0 36.0 41.0 33.0 41.0 46-47 37.41893180301909 40.0 36.0 41.0 33.0 41.0 48-49 37.25737739952778 40.0 35.0 41.0 33.0 41.0 50-51 37.00512573517699 40.0 35.0 41.0 32.0 41.0 52-53 36.492250378924695 40.0 35.0 41.0 30.5 41.0 54-55 35.786062806865914 39.5 35.0 41.0 28.5 41.0 56-57 35.22295114513328 39.0 35.0 41.0 25.5 41.0 58-59 34.93750512593233 39.0 35.0 41.0 24.0 41.0 60-61 34.60885262313673 39.0 35.0 41.0 22.5 41.0 62-63 34.3323275991237 38.0 35.0 41.0 22.0 41.0 64-65 34.044906025888324 38.0 34.0 40.0 21.0 41.0 66-67 33.697117333379595 37.0 34.0 40.0 19.5 41.0 68-69 33.378434571811155 37.0 34.0 40.0 18.5 41.0 70-71 33.04406862598201 36.0 34.0 39.0 17.5 41.0 72-73 32.76698388958899 36.0 33.5 39.0 16.0 41.0 74-75 32.48643776881572 35.0 33.0 39.0 13.0 40.5 76-77 32.22038433451992 35.0 33.0 37.5 9.5 40.0 78-79 31.88638656863781 35.0 33.0 37.0 7.0 39.0 80-81 31.541129100943017 35.0 33.0 36.5 2.0 39.0 82-83 31.189611194089878 35.0 33.0 36.0 2.0 38.5 84-85 30.8845614410021 35.0 33.0 36.0 2.0 37.0 86-87 30.705886817836983 35.0 33.0 35.5 2.0 37.0 88-89 30.535718580794946 35.0 33.0 35.0 2.0 36.5 90-91 30.32888188826729 35.0 33.0 35.0 2.0 36.0 92-93 30.166006472869622 35.0 33.0 35.0 2.0 36.0 94-95 29.976680458053313 35.0 32.5 35.0 2.0 36.0 96-97 29.795719866221052 35.0 32.0 35.0 2.0 36.0 98-99 29.643087640825133 35.0 32.0 35.0 2.0 35.5 100 29.498924342816046 35.0 32.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 3.9996520314399397 1101 2 4.10442253031697 1101 3 3.432448004502966 1101 4 1.938306916137492 1101 5 1.0664646113179828 1101 6 0.8599026859406607 1101 7 0.4760591134584047 1101 8 0.5109329172089332 1101 9 0.5987202558864908 1101 10-11 0.4395443133538066 1101 12-13 0.42557390093312364 1101 14-15 0.50460547469023 1101 16-17 0.518421173578453 1101 18-19 0.46785114660855953 1101 20-21 0.512857937673715 1101 22-23 0.4813041349160443 1101 24-25 0.5051882385424022 1101 26-27 0.46611388190762426 1101 28-29 0.5107801362841613 1101 30-31 0.5408009522321393 1101 32-33 0.5100824666370869 1101 34-35 0.5392967470533421 1101 36-37 0.5299895338411531 1101 38-39 0.49423474253924127 1101 40-41 0.48741584017580664 1101 42-43 0.4962005529188218 1101 44-45 0.47829402282953737 1101 46-47 0.4933978835375967 1101 48-49 0.5382400192490806 1101 50-51 0.5143366643957421 1101 52-53 0.47067393254514656 1101 54-55 0.4822928076726143 1101 56-57 0.4233633540127997 1101 58-59 0.3910790857194826 1101 60-61 0.3545043637456473 1101 62-63 0.35647826114569625 1101 64-65 0.375127671763849 1101 66-67 0.29870987348885336 1101 68-69 0.31684793428538427 1101 70-71 0.3854051139261472 1101 72-73 0.3360113306266186 1101 74-75 0.32755401643700566 1101 76-77 0.31557446600040606 1101 78-79 0.3083648144102362 1101 80-81 0.2950449417374763 1101 82-83 0.2981971723262582 1101 84-85 0.3218738839777586 1101 86-87 0.37466037997484136 1101 88-89 0.4234840549647245 1101 90-91 0.4752542547832448 1101 92-93 0.444827484804744 1101 94-95 0.4835250347706648 1101 96-97 0.4744004858617359 1101 98-99 0.48006991512926334 1101 100 0.5255838647733775 1102 1 2.9323640454391473 1102 2 3.487466387788725 1102 3 4.211732357321171 1102 4 2.1874280294893467 1102 5 1.1303317516083453 1102 6 0.8964958095049766 1102 7 0.47037990708497546 1102 8 0.5166550496606916 1102 9 0.603032431765314 1102 10-11 0.43327440575467335 1102 12-13 0.3815071991101249 1102 14-15 0.5168772987541459 1102 16-17 0.6007588084117188 1102 18-19 0.4839309481238345 1102 20-21 0.4612678021808563 1102 22-23 0.3911714967498625 1102 24-25 0.4450251194447432 1102 26-27 0.38439221759248454 1102 28-29 0.4133566850795134 1102 30-31 0.4584021245581411 1102 32-33 0.41171073785962875 1102 34-35 0.43366657465610103 1102 36-37 0.4203372903801821 1102 38-39 0.4347752760458192 1102 40-41 0.4368190229192308 1102 42-43 0.500674941876909 1102 44-45 0.4622054376529121 1102 46-47 0.5165644812904802 1102 48-49 0.4757587129950167 1102 50-51 0.5042109571890094 1102 52-53 0.4795417543825664 1102 54-55 0.48518637344547955 1102 56-57 0.46194480571779195 1102 58-59 0.35284285630456225 1102 60-61 0.393593999057785 1102 62-63 0.36744612628331197 1102 64-65 0.37369643921121565 1102 66-67 0.3083044945047675 1102 68-69 0.3174640918629663 1102 70-71 0.3582861613549895 1102 72-73 0.34323948688079753 1102 74-75 0.3668471968348683 1102 76-77 0.3450692444390526 1102 78-79 0.38537500410803815 1102 80-81 0.3637636072455628 1102 82-83 0.3518046139092128 1102 84-85 0.38159660005501905 1102 86-87 0.4421267556312145 1102 88-89 0.44076236267199675 1102 90-91 0.4182918042848698 1102 92-93 0.4274154946515516 1102 94-95 0.44113105112323936 1102 96-97 0.4312247807533254 1102 98-99 0.48513115839471865 1102 100 0.5140326831919992 1103 1 2.6810477538366086 1103 2 3.3025316767608253 1103 3 3.082901854741465 1103 4 1.7184051455380427 1103 5 0.9183903789246202 1103 6 0.7598142499799039 1103 7 0.40977850044937014 1103 8 0.46581020258801686 1103 9 0.5551416532395024 1103 10-11 0.4115331629018044 1103 12-13 0.3989948343863503 1103 14-15 0.496870877881058 1103 16-17 0.5058147538019924 1103 18-19 0.4717182910282389 1103 20-21 0.45238852847650435 1103 22-23 0.427741637468948 1103 24-25 0.4565976137267782 1103 26-27 0.3966880381189881 1103 28-29 0.43756724453846374 1103 30-31 0.4636776706226087 1103 32-33 0.42718330048964503 1103 34-35 0.4295201078273294 1103 36-37 0.4201901453719259 1103 38-39 0.46417701927247634 1103 40-41 0.47304256521193366 1103 42-43 0.436514481128782 1103 44-45 0.380291288057677 1103 46-47 0.42662001275654404 1103 48-49 0.47669332357057925 1103 50-51 0.5046197388939646 1103 52-53 0.5323083834090525 1103 54-55 0.44605971440443426 1103 56-57 0.358078318233197 1103 58-59 0.3040434636626159 1103 60-61 0.35545583645725287 1103 62-63 0.36689307053831755 1103 64-65 0.35101014303026545 1103 66-67 0.3579569748446332 1103 68-69 0.3776875718146542 1103 70-71 0.3584183520892452 1103 72-73 0.3099426916132586 1103 74-75 0.3343863265541671 1103 76-77 0.35235841189108186 1103 78-79 0.35295577864100025 1103 80-81 0.3637209844097704 1103 82-83 0.4105179014820486 1103 84-85 0.41049093908620193 1103 86-87 0.4036276713123321 1103 88-89 0.40174133002195944 1103 90-91 0.41618252602230044 1103 92-93 0.4276040951788538 1103 94-95 0.40463441981007264 1103 96-97 0.3710092196260568 1103 98-99 0.35873504756600383 1103 100 0.37210116392357406 1104 1 2.514454760707597 1104 2 3.3262233697745813 1104 3 1.7514086771265696 1104 4 1.1665315169041435 1104 5 0.6407939512572653 1104 6 0.5833410350856312 1104 7 0.3425141518338535 1104 8 0.3825910130748582 1104 9 0.44641788890700695 1104 10-11 0.35483095288040545 1104 12-13 0.3364680806843765 1104 14-15 0.42118107189507725 1104 16-17 0.4565477319820559 1104 18-19 0.3777820462466437 1104 20-21 0.37572717295050495 1104 22-23 0.33519465828677397 1104 24-25 0.38779894826513583 1104 26-27 0.37322448411389786 1104 28-29 0.3853688211850823 1104 30-31 0.3235364368743632 1104 32-33 0.29613858817410943 1104 34-35 0.33252259458392786 1104 36-37 0.3301807070480649 1104 38-39 0.30792164066364336 1104 40-41 0.297985926987856 1104 42-43 0.37036197177805263 1104 44-45 0.2941969832965725 1104 46-47 0.27824445932118635 1104 48-49 0.3276617629927401 1104 50-51 0.4064898433646249 1104 52-53 0.35146842290770763 1104 54-55 0.37217838701059947 1104 56-57 0.42844166121527394 1104 58-59 0.41929645303846996 1104 60-61 0.3749441327318266 1104 62-63 0.3870059144683111 1104 64-65 0.35472764158206616 1104 66-67 0.35878391903297313 1104 68-69 0.32512505924200497 1104 70-71 0.3072660542201504 1104 72-73 0.2590146630209489 1104 74-75 0.3114299144498247 1104 76-77 0.30736406775108094 1104 78-79 0.34364413674210326 1104 80-81 0.3956492425106326 1104 82-83 0.36068084267447986 1104 84-85 0.35458704838450217 1104 86-87 0.313022095216585 1104 88-89 0.3500222279042262 1104 90-91 0.37179596069950804 1104 92-93 0.3504874728617544 1104 94-95 0.3685061888068901 1104 96-97 0.3836546435136228 1104 98-99 0.3606421883966 1104 100 0.4239936475474444 1105 1 1.4267310264055055 1105 2 2.659257674096356 1105 3 1.485630901314913 1105 4 0.9303339349129303 1105 5 0.5698514333482265 1105 6 0.47561198143535677 1105 7 0.19189558585743072 1105 8 0.2847658480259625 1105 9 0.4102718772867391 1105 10-11 0.2553388847529021 1105 12-13 0.24633416194055258 1105 14-15 0.3189121394759127 1105 16-17 0.395684406609071 1105 18-19 0.2849793910408849 1105 20-21 0.31392325480533145 1105 22-23 0.2823741895978955 1105 24-25 0.3469351101800626 1105 26-27 0.27787321856636993 1105 28-29 0.2658887946309889 1105 30-31 0.2503968076126242 1105 32-33 0.24602057528068855 1105 34-35 0.2495538591690547 1105 36-37 0.19146114328172104 1105 38-39 0.16949662378885222 1105 40-41 0.0771850477836793 1105 42-43 0.156166358333941 1105 44-45 0.18000438374664895 1105 46-47 0.15712404050000828 1105 48-49 0.11952616938941674 1105 50-51 0.19905048477731668 1105 52-53 0.13383295210385882 1105 54-55 0.16650718497952965 1105 56-57 0.10720946574739543 1105 58-59 0.0778642683467794 1105 60-61 0.12409718709398021 1105 62-63 -0.2156246216009876 1105 64-65 -0.1361881249131187 1105 66-67 -0.001012190230142096 1105 68-69 0.08302877394773844 1105 70-71 0.09459396110119656 1105 72-73 0.048184729176711016 1105 74-75 -0.0355486789132442 1105 76-77 -0.026536038938225204 1105 78-79 -0.03307801178062775 1105 80-81 0.07947861498126585 1105 82-83 0.04901666323963383 1105 84-85 0.054354423174146405 1105 86-87 0.09436511478783416 1105 88-89 0.08747246165287947 1105 90-91 0.12498453025600043 1105 92-93 0.04180745444230993 1105 94-95 0.05282297193600982 1105 96-97 0.03251296162127204 1105 98-99 0.06358176637439428 1105 100 0.11537876043146511 1106 1 1.8099064828708649 1106 2 2.842208273985065 1106 3 1.1881437793163876 1106 4 0.8382790028742875 1106 5 0.5566435500263367 1106 6 0.5293347244903472 1106 7 0.2947991969043642 1106 8 0.38253778293144336 1106 9 0.3900730207686678 1106 10-11 0.27609012048785075 1106 12-13 0.20813986438143672 1106 14-15 0.19727603941962002 1106 16-17 0.2668675801130931 1106 18-19 0.07069021930056607 1106 20-21 0.219343364012083 1106 22-23 0.24075497819041658 1106 24-25 0.29354326586801704 1106 26-27 0.25545101536241077 1106 28-29 0.2662011858228581 1106 30-31 0.2736166659302057 1106 32-33 0.20442048156333215 1106 34-35 0.27180908765392076 1106 36-37 0.27958332240553574 1106 38-39 0.23527164681143375 1106 40-41 0.18192721115776322 1106 42-43 0.20918683134627258 1106 44-45 0.1699582409643483 1106 46-47 0.17964325581238683 1106 48-49 0.11592777267212995 1106 50-51 0.22642457648365877 1106 52-53 0.16839753247462852 1106 54-55 0.1444982492898177 1106 56-57 0.14125502116917232 1106 58-59 0.1683958865656976 1106 60-61 -0.04038897820651499 1106 62-63 0.08746418698456182 1106 64-65 -0.10551521476138248 1106 66-67 -0.1892528990327449 1106 68-69 0.10173584049682205 1106 70-71 0.14219538248111974 1106 72-73 0.09398644374878984 1106 74-75 0.1610330374160185 1106 76-77 0.14353106822171213 1106 78-79 0.13210798300634252 1106 80-81 0.1015597504581649 1106 82-83 0.09911904457165832 1106 84-85 0.12072209459067551 1106 86-87 0.15264783369758916 1106 88-89 0.12416866447286168 1106 90-91 0.09754373001389638 1106 92-93 0.1296162254758393 1106 94-95 0.1467654708052173 1106 96-97 0.115492482100791 1106 98-99 0.10954191371891042 1106 100 0.07470973889925148 1107 1 1.8957645647561883 1107 2 1.1282098874554656 1107 3 0.728918318813971 1107 4 0.5106249914802063 1107 5 0.335651479289659 1107 6 0.41524826548334204 1107 7 0.2194780619918575 1107 8 0.24076109575180737 1107 9 0.2712150531592812 1107 10-11 0.1996507948998385 1107 12-13 0.18903271034238145 1107 14-15 0.2205525642568702 1107 16-17 0.2883081382307253 1107 18-19 0.22178860855162696 1107 20-21 0.2921918092356748 1107 22-23 0.2222550174373339 1107 24-25 0.16315638228874008 1107 26-27 0.2207800802941975 1107 28-29 0.2288262767112812 1107 30-31 0.24562093546568775 1107 32-33 0.17125403267611716 1107 34-35 0.1640927325624233 1107 36-37 0.17318758200013917 1107 38-39 0.2270312902453142 1107 40-41 0.2517143416713452 1107 42-43 0.26818135082603334 1107 44-45 0.2930258342362535 1107 46-47 0.27888158369714233 1107 48-49 0.23303474030407045 1107 50-51 0.2773428220670553 1107 52-53 0.3293856095381855 1107 54-55 0.19487733797764406 1107 56-57 0.2636665278740935 1107 58-59 0.2802916210493933 1107 60-61 0.2900235150890609 1107 62-63 0.2870036829911413 1107 64-65 0.26829435314579797 1107 66-67 0.2163611063968176 1107 68-69 0.22590144189000938 1107 70-71 0.2677233417207674 1107 72-73 0.27409425843379154 1107 74-75 0.32508706731852044 1107 76-77 0.2971343148300676 1107 78-79 0.29448099434359776 1107 80-81 0.28706486679472576 1107 82-83 0.34133379948505294 1107 84-85 0.36473145891786274 1107 86-87 0.3855523598058639 1107 88-89 0.38875572957071114 1107 90-91 0.39789717906201005 1107 92-93 0.3840751289131745 1107 94-95 0.4029837685246491 1107 96-97 0.37206494703023907 1107 98-99 0.4310420650998772 1107 100 0.474561258926709 1108 1 1.3326424898947558 1108 2 -0.6540593965096697 1108 3 0.11683932350863913 1108 4 0.09364356988356093 1108 5 0.07988684246628708 1108 6 0.10320440524041175 1108 7 0.10364316591052614 1108 8 0.03782547547205439 1108 9 0.11645788780229083 1108 10-11 0.07795462698996403 1108 12-13 0.12118500474537797 1108 14-15 0.12770375919097887 1108 16-17 0.16152256400400944 1108 18-19 0.14660407356591776 1108 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-0.32602609750436784 2215 10-11 -0.39930057577240774 2215 12-13 -0.3559375799133946 2215 14-15 -0.5283846042845042 2215 16-17 -0.5225737394369148 2215 18-19 -0.5630754715303397 2215 20-21 -0.46919147071839973 2215 22-23 -0.4649047968171871 2215 24-25 -0.4677529379281893 2215 26-27 -0.6823125254446083 2215 28-29 -0.6661221430222142 2215 30-31 -0.6666159275632566 2215 32-33 -0.731590898361091 2215 34-35 -0.8558023226748759 2215 36-37 -0.8568742417694253 2215 38-39 -0.9054752150223848 2215 40-41 -0.8736667600883763 2215 42-43 -0.9578423351394179 2215 44-45 -1.0448430447065036 2215 46-47 -0.997626539609584 2215 48-49 -1.0222248355891779 2215 50-51 -0.9000637178307187 2215 52-53 -1.0020823137660884 2215 54-55 -1.1451212616340385 2215 56-57 -1.0673614710342747 2215 58-59 -1.0598688921338706 2215 60-61 -1.1495815247903707 2215 62-63 -1.0322983331040447 2215 64-65 -1.1069251092147567 2215 66-67 -1.058782608453754 2215 68-69 -1.016632856024053 2215 70-71 -1.0068103943507438 2215 72-73 -1.0991012294475802 2215 74-75 -1.0381124909686363 2215 76-77 -1.017739943375382 2215 78-79 -1.0879320856206824 2215 80-81 -1.0511370093038117 2215 82-83 -1.113496709239751 2215 84-85 -1.0904562922398817 2215 86-87 -1.1118818757234266 2215 88-89 -1.1265853641072532 2215 90-91 -1.1407284326060925 2215 92-93 -1.1566362177331264 2215 94-95 -1.2965997509295661 2215 96-97 -1.3230054330275536 2215 98-99 -1.3315289719171908 2215 100 -1.3321514433967145 2216 1 -3.356160441532449 2216 2 -4.399381149172697 2216 3 -5.993274487893377 2216 4 -3.356838311105246 2216 5 -1.6586420956825378 2216 6 -0.858687217534893 2216 7 -0.6798725830074304 2216 8 -0.3925682030576496 2216 9 -0.31261549018294943 2216 10-11 -0.4367571275371205 2216 12-13 -0.44562488497693664 2216 14-15 -0.6357776938264976 2216 16-17 -0.5489316124495645 2216 18-19 -0.5999609735886224 2216 20-21 -0.5048096891763336 2216 22-23 -0.5429669338294119 2216 24-25 -0.5761638332500425 2216 26-27 -0.7328599591156646 2216 28-29 -0.7056005558579628 2216 30-31 -0.8405863260210467 2216 32-33 -0.9062136320938663 2216 34-35 -0.8699387074238487 2216 36-37 -0.918056318093214 2216 38-39 -1.0290197445869538 2216 40-41 -1.1307199560148646 2216 42-43 -1.2131103123918763 2216 44-45 -1.180060587160014 2216 46-47 -1.2893861650537843 2216 48-49 -1.2228330679895905 2216 50-51 -1.2432600078949605 2216 52-53 -1.2130932361313782 2216 54-55 -1.11560164226848 2216 56-57 -1.1833659041983609 2216 58-59 -1.191731405221546 2216 60-61 -1.1713081384404518 2216 62-63 -1.2492862816136494 2216 64-65 -1.2888415988939244 2216 66-67 -1.3047722657659264 2216 68-69 -1.2663301124216488 2216 70-71 -1.1807304397118301 2216 72-73 -1.1423451600938108 2216 74-75 -1.1937736848100826 2216 76-77 -1.2039257759601227 2216 78-79 -1.1656078363520201 2216 80-81 -1.1541949611182751 2216 82-83 -1.0973219350545556 2216 84-85 -1.1310552596051444 2216 86-87 -1.1756161751041603 2216 88-89 -1.2089474987092572 2216 90-91 -1.256861056600492 2216 92-93 -1.2484773738526478 2216 94-95 -1.2562821579432182 2216 96-97 -1.3272741304853497 2216 98-99 -1.3957486153440648 2216 100 -1.441920769349064 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3740.0 3 81.0 4 169.0 5 355.0 6 645.0 7 1101.0 8 1768.0 9 2730.0 10 4472.0 11 7988.0 12 8574.0 13 11346.0 14 12288.0 15 14847.0 16 16145.0 17 21793.0 18 31674.0 19 52086.0 20 91878.0 21 98099.0 22 47331.0 23 31413.0 24 30042.0 25 31563.0 26 35302.0 27 42535.0 28 53006.0 29 65493.0 30 79963.0 31 99113.0 32 123551.0 33 160911.0 34 224744.0 35 358402.0 36 561876.0 37 951940.0 38 1296100.0 39 494513.0 40 2671.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.572992550309618 39.34612633651019 26.680272272507572 17.400608840672625 2 18.740547858447883 37.278615317211404 26.650021038717153 17.330815785623557 3 19.731132678121053 25.01569417958873 21.86700010883401 33.38617303345621 4 31.570045470962775 25.950130987286112 23.876356203403304 18.60346733834781 5 17.51906919008781 38.10778114437222 24.654124481275446 19.719025184264527 6 34.086892095519126 23.329171430379606 25.154198405859074 17.429738068242195 7 18.824950992965423 23.3820975424818 39.2571631694219 18.53578829513088 8 33.00349273142796 23.154999518950966 25.63413697437507 18.207370775246005 9 32.681682756836814 24.595721660297368 25.565291760182074 17.157303822683748 10-11 17.139027902421176 32.47228842122861 32.54668344292313 17.842000233427072 12-13 17.12928863099754 24.935866700524105 32.28478773119926 25.650056937279093 14-15 17.388197501384 40.15057031911689 24.33129255509589 18.129939624403224 16-17 25.280339210543335 24.473980767501903 24.47624800680093 25.769432015153832 18-19 17.77606300007413 32.32129028391356 24.567450171994746 25.335196544017563 20-21 25.495510077582956 32.20210249972005 24.755936618241066 17.546450804455933 22-23 25.012804973258408 24.581585916146057 32.6085495031 17.797059607495534 24-25 17.41845919112606 32.11293246647731 24.800020188480364 25.668588153916268 26-27 17.811478905354008 27.89461263243856 36.57697677220085 17.716931690006582 28-29 25.098048957348183 24.36028390774753 32.84810115352062 17.69356598138366 30-31 22.824167397527724 25.767014736268496 33.283644310319346 18.125173555884427 32-33 19.5502968496233 27.107711189591903 33.874719354496854 19.46727260628794 34-35 19.80800072241998 26.71898099349309 34.06788894448999 19.405129339596936 36-37 19.736865323535362 26.375454985391865 34.84837894926531 19.039300741807452 38-39 20.573536338505466 26.255143692149176 34.06779864861694 19.10352132072842 40-41 19.219613375408752 27.214386480659403 34.45823955582239 19.107760588109453 42-43 19.32190129244611 26.555474704487487 34.311502000447774 19.811122002618628 44-45 19.356209983459554 27.95360590274594 33.47651695874194 19.213667155052566 46-47 20.098234457400583 26.543050554435823 33.25915945959908 20.099555528564515 48-49 19.394126538868193 27.527318767793336 33.316129085688395 19.762425607650083 50-51 20.293940947274344 27.58081842292434 33.01241140330536 19.112829226495954 52-53 20.21619621133634 27.18976571234837 33.109790524367845 19.484247551947455 54-55 19.963274632287924 28.339081591752002 31.243830061331185 20.453813714628886 56-57 20.19907516757511 28.942278611387025 30.609670165183573 20.248976055854296 58-59 21.645509184869848 28.843863866936825 29.401846053503395 20.108780894689932 60-61 20.80844786343089 29.505952252432643 29.381185998140385 20.30441388599608 62-63 19.95858311056515 29.591120360872132 29.389566709930442 21.060729818632275 64-65 20.744713573522557 29.482420773599532 29.503804271099764 20.269061381778144 66-67 20.90279545460371 28.579219597737985 30.163144259921932 20.35484068773637 68-69 21.429915658546996 28.636069908126917 29.825791597597963 20.108222835728125 70-71 19.759481083869023 29.39141667735436 30.508049680665554 20.341052558111063 72-73 19.65843258239999 28.82468718996989 30.4686058286055 21.04827439902462 74-75 19.888001764937773 29.948176640066748 29.549310955636322 20.614510639359157 76-77 20.640522176111016 28.72602463034229 29.395631071071616 21.237822122475077 78-79 20.348095245136456 29.575961002922806 28.96972206203453 21.106221689906203 80-81 20.800116478940982 29.578829459590356 29.071599948069476 20.549454113399186 82-83 20.8041250080833 29.11162134496698 29.55091928841698 20.533334358532734 84-85 20.20619487590375 29.74642915725588 28.831282412022258 21.216093554818112 86-87 20.337842240462976 29.89189446552934 29.152198559230335 20.61806473477735 88-89 21.88926777469736 29.0702502394433 28.48698055393123 20.55350143192811 90-91 21.140885446470616 29.893570050685014 28.451565338537378 20.513979164306996 92-93 20.582363353701435 29.98205818211857 28.265150120898536 21.170428343281458 94-95 20.978566701848468 30.25491083842996 28.176838387181007 20.58968407254056 96-97 20.964150558388546 29.304875512229938 28.85978133684004 20.87119259254148 98-99 21.699840666615472 29.569439062299914 27.793664893590282 20.937055377494335 100 20.533870742289547 29.44466930678937 29.01988648405566 21.001573466865423 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 28086.0 1 16719.0 2 4123.0 3 2453.5 4 1846.0 5 1610.0 6 1594.0 7 1656.0 8 1598.0 9 1551.5 10 1723.5 11 2824.0 12 17947.0 13 20576.0 14 8927.5 15 9851.5 16 11977.5 17 14383.0 18 17169.0 19 20183.5 20 23434.5 21 27391.0 22 31922.5 23 37582.5 24 44602.5 25 52017.0 26 61852.0 27 73210.5 28 82088.5 29 89299.5 30 97182.5 31 106405.0 32 116316.5 33 126457.5 34 137470.0 35 146519.5 36 153434.5 37 158358.0 38 159879.0 39 159290.0 40 159552.0 41 160582.5 42 160701.0 43 159997.5 44 158770.0 45 159608.5 46 164547.5 47 171985.5 48 173079.0 49 179468.5 50 217271.5 51 206742.0 52 144408.5 53 115268.5 54 104940.0 55 97164.5 56 86487.5 57 74397.0 58 69026.5 59 58687.0 60 43525.0 61 34883.5 62 28104.5 63 21185.5 64 15520.5 65 11806.0 66 8970.0 67 6954.5 68 5453.0 69 4170.5 70 3027.5 71 2289.0 72 1840.5 73 1328.5 74 896.5 75 659.0 76 477.5 77 333.5 78 179.0 79 117.5 80 100.5 81 59.5 82 36.5 83 35.5 84 35.5 85 27.5 86 16.5 87 14.0 88 12.5 89 11.0 90 8.5 91 5.5 92 2.5 93 0.5 94 0.5 95 2.0 96 1.5 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025609946516810693 2 0.012735970323217632 3 0.00599339779916124 4 0.0 5 0.003785303873154467 6 9.66041092627963E-4 7 3.3515711376888514E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.561835304582898E-4 26-27 0.003529007256742967 28-29 7.294596005558089E-4 30-31 6.801717897074433E-4 32-33 0.004642911781916026 34-35 0.008004340481774551 36-37 0.004820347900970141 38-39 0.005106217203890661 40-41 0.007945195108756511 42-43 0.010025140726557535 44-45 0.008654939584972975 46-47 0.011947365349643788 48-49 0.016284692704299948 50-51 0.059115800331529536 52-53 0.01010400122391492 54-55 0.005461089441998893 56-57 0.0038247341218331592 58-59 0.007915622422247493 60-61 0.015072212557430157 62-63 0.012726112761047962 64-65 0.011434772116820787 66-67 0.009443544558546822 68-69 0.006022970485670259 70-71 0.004228894170789756 72-73 0.0031544198942953894 74-75 0.0022573817368551382 76-77 0.0017645036283714835 78-79 0.0014392040767722714 80-81 0.001705358255353445 82-83 0.0012617679577181557 84-85 0.0011040469630033863 86-87 5.618810436713662E-4 88-89 0.0013011982063968481 90-91 7.59032287064828E-4 92-93 3.844449246172506E-4 94-95 4.6330542197463526E-4 96-97 0.0012420528333788095 98-99 0.003519149694573294 100 1.7743611905411566E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 5072248.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.9844084090915 #Duplication Level Percentage of deduplicated Percentage of total 1 86.19726451496031 56.87675505501632 2 9.940598330661294 13.11849000162176 3 2.1738359048510776 4.303178284801215 4 0.6313170946371796 1.666283400327229 5 0.27235192484380477 0.8985490319947905 6 0.16158269126402733 0.6397162975323433 7 0.1065414272063132 0.49210511316882044 8 0.0812512780071305 0.42890540094265067 9 0.055141570246460075 0.3274635502314927 >10 0.27102676972679923 3.6121239980540953 >50 0.08651934447888401 3.8748175183216755 >100 0.018697226745147826 2.163275282701536 >500 0.0014296337234639366 0.6764695531097447 >1k 0.0021444485691849056 2.85979705097117 >5k 1.1913603162099856E-4 0.513724229035048 >10k+ 1.7870404743149785E-4 7.5483462321701005 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 267851 5.280715769418214 No Hit AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG 45713 0.901237478924532 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 27939 0.5508208589169931 No Hit TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 22389 0.44140191883362173 No Hit AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA 10162 0.20034509353643593 No Hit AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10031 0.19776241224798158 No Hit ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 9336 0.18406040083213596 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5767 0.11369712206500944 No Hit AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG 5621 0.11081871391146489 No Hit ACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 5416 0.10677711342189894 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 3.9430248678692367E-4 1.9715124339346182E-5 0.0 0.0 0.0 2 3.9430248678692367E-4 1.9715124339346182E-5 0.0 0.0 0.0 3 4.534478598049622E-4 1.9715124339346182E-5 0.0 0.0 0.0 4 4.534478598049622E-4 1.9715124339346182E-5 0.0 0.0 0.0 5 4.534478598049622E-4 3.9430248678692364E-5 0.0 0.0 0.0 6 4.534478598049622E-4 5.914537301803855E-5 0.0 0.0 0.0 7 4.534478598049622E-4 9.857562169673092E-5 0.0 0.0 0.0 8 4.534478598049622E-4 9.857562169673092E-5 0.0 0.0 0.0 9 4.534478598049622E-4 9.857562169673092E-5 0.0 0.0 0.0 10-11 4.633054219746353E-4 9.857562169673092E-5 0.0 0.0 0.0 12-13 4.731629841443084E-4 9.857562169673092E-5 0.0 0.0 0.0 14-15 4.731629841443084E-4 9.857562169673092E-5 0.0 0.0 0.0 16-17 4.731629841443084E-4 1.0843318386640402E-4 0.0 0.0 0.0 18-19 5.027356706533276E-4 1.3800587037542328E-4 0.0 0.0 0.0 20-21 5.618810436713662E-4 1.5772099471476946E-4 0.0 0.0 0.0 22-23 6.013112923500586E-4 1.9715124339346184E-4 0.0 0.0 0.0 24-25 6.505991031984241E-4 2.0700880556313492E-4 0.0 0.0 0.0 26-27 7.097444762164625E-4 2.267239299024811E-4 0.0 0.0 0.0 28-29 7.688898492345011E-4 2.464390542418273E-4 0.0 0.0 0.0 30-31 8.378927844222128E-4 2.9572686509019274E-4 0.0 0.0 0.0 32-33 0.001064616714324694 3.0558442725986585E-4 0.0 0.0 0.0 34-35 0.0012913406442271748 3.3515711376888514E-4 0.0 0.0 0.0 36-37 0.001488491887620637 3.548722381082313E-4 0.0 0.0 0.0 38-39 0.002020800244782984 3.8444492461725056E-4 0.0 0.0 0.0 40-41 0.0025531086019453305 4.041600489565968E-4 0.0 0.0 0.0 42-43 0.0031544198942953894 4.33732735465616E-4 0.0 0.0 0.0 44-45 0.003933167305699563 4.4359029763528914E-4 0.0 0.0 0.0 46-47 0.005175220139078373 4.534478598049622E-4 0.0 0.0 0.0 48-49 0.006653854464529338 4.928781084836545E-4 0.0 0.0 0.0 50-51 0.008645082022803303 4.928781084836545E-4 0.0 0.0 0.0 52-53 0.011060184754373209 5.224507949926738E-4 0.0 0.0 0.0 54-55 0.01415545927565056 5.323083571623469E-4 0.0 0.0 0.0 56-57 0.018965949614451028 5.520234815016931E-4 0.0 0.0 0.0 58-59 0.024269318061735153 5.815961680107124E-4 0.0 0.0 0.0 60-61 0.03209622242445559 6.407415410287509E-4 0.0 0.0 0.0 62-63 0.041953784594128685 6.998869140467895E-4 0.0 0.0 0.0 64-65 0.055409356955732444 7.688898492345011E-4 0.0 1.9715124339346182E-5 0.0 66-67 0.0730149629907686 7.984625357435205E-4 0.0 1.9715124339346182E-5 0.0 68-69 0.09757015035542427 8.674654709312322E-4 0.0 1.9715124339346182E-5 0.0 70-71 0.13167731546249314 9.167532817795975E-4 0.0 1.9715124339346182E-5 0.0 72-73 0.17414369328944485 9.266108439492706E-4 0.0 1.9715124339346182E-5 0.0 74-75 0.2307852455163864 9.364684061189438E-4 0.0 1.9715124339346182E-5 0.0 76-77 0.30109923647266457 9.75898654797636E-4 0.0 1.9715124339346182E-5 0.0 78-79 0.3912860727630037 0.0010153289034763285 0.0 1.9715124339346182E-5 0.0 80-81 0.5050324826388615 0.0010449015899853477 0.0 1.9715124339346182E-5 0.0 82-83 0.6448521444535047 0.0010646167143246938 0.0 2.9572686509019273E-5 0.0 84-85 0.8120265412889907 0.001074474276494367 0.0 3.9430248678692364E-5 0.0 86-87 1.0099861047803658 0.0011139045251730593 0.0 3.9430248678692364E-5 0.0 88 1.1806007908130676 0.0011237620873427324 0.0 3.9430248678692364E-5 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 5960 0.0 86.616585 1 ATCGAGT 2940 0.0 79.79913 1 TCGAGTG 3480 0.0 73.348755 2 AGTGGTA 120155 0.0 60.64441 5 TGGTATC 122710 0.0 59.51016 7 GTGGTAT 122475 0.0 59.502144 6 GGTATCA 123505 0.0 59.172756 8 GTATCAA 124570 0.0 58.80267 9 GAGTGGT 125345 0.0 58.144627 4 ACGAGTG 117180 0.0 57.99985 2 CGAGTGG 121510 0.0 57.937744 3 AACGAGT 109515 0.0 56.8965 1 GACGAGT 1540 0.0 53.42718 1 ATGAGTG 5440 0.0 49.946045 2 TGAGTGG 5725 0.0 49.913597 3 AATGAGT 5625 0.0 44.13228 1 GAGTACT 47125 0.0 40.49783 20-21 AGTACTT 48405 0.0 39.99488 20-21 GTACTTT 48095 0.0 38.909317 22-23 CATGGGG 23665 0.0 37.731087 24-25 >>END_MODULE