FastQCFastQC Report
Thu 26 May 2016
SRR2132633_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132633_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4237410
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2201155.19456460432198No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA962102.270490700687448No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG436561.0302519699533441No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262100.6185382108410562No Hit
TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT183880.43394431976136366No Hit
AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT84480.199367066203176No Hit
ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT79250.1870246211718951No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63520.14990288879291833No Hit
ACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT60500.14277589376529531No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA52630.12420322791516515No Hit
AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG49790.11750102067064551No Hit
CGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT42380.10001392359955728No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT49400.086.707691
ATCGAGT20100.083.74391
TCGAGTG27400.070.336632
AGTGGTA1102500.055.4938435
GTGGTAT1132900.054.028356
TGGTATC1137650.053.9384467
GGTATCA1139300.053.8885658
GTATCAA1145450.053.6894879
GAGTGGT1154700.052.875284
ACGAGTG1081950.052.764112
GACGAGT14350.052.752151
CGAGTGG1119600.052.689183
AACGAGT1009850.051.792911
ATGAGTG43150.050.7637182
AATGAGT40750.048.6912651
TGAGTGG51400.046.7228363
AGTACTT398450.039.6755420-21
GAGTACT390250.039.38328620-21
GTACTTT395550.038.29719522-23
CATGGGG209550.037.83276724-25
Produced by FastQC (version 0.11.5)