##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132633_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4237410 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.492204436200414 31.0 16.0 33.0 10.0 34.0 2 27.802107183397407 31.0 25.0 34.0 16.0 34.0 3 28.013443117375946 31.0 25.0 34.0 16.0 34.0 4 33.843237968476025 35.0 33.0 37.0 28.0 37.0 5 34.88085741054087 35.0 35.0 37.0 32.0 37.0 6 35.29101243448238 37.0 35.0 37.0 33.0 37.0 7 35.56669498585221 37.0 35.0 37.0 33.0 37.0 8 35.66731470402911 37.0 35.0 37.0 35.0 37.0 9 37.458843255667965 39.0 37.0 39.0 35.0 39.0 10-11 37.493649304646 39.0 37.0 39.0 34.5 39.0 12-13 37.64968188114909 39.0 37.0 39.0 35.0 39.0 14-15 39.10864018350832 40.5 39.0 41.0 36.0 41.0 16-17 38.99677739468213 40.5 38.5 41.0 35.5 41.0 18-19 38.90320490582691 40.0 38.0 41.0 35.0 41.0 20-21 38.81276912076009 40.0 38.0 41.0 35.0 41.0 22-23 38.66125581428278 40.0 38.0 41.0 35.0 41.0 24-25 38.5197066132378 40.0 38.0 41.0 34.0 41.0 26-27 38.57032585942828 40.0 38.0 41.0 34.0 41.0 28-29 38.50851805702068 40.0 38.0 41.0 34.0 41.0 30-31 38.37887896144107 40.0 38.0 41.0 34.0 41.0 32-33 38.2501487937207 40.0 38.0 41.0 34.0 41.0 34-35 38.14382240566761 40.0 38.0 41.0 33.5 41.0 36-37 37.82682794442832 40.0 37.5 41.0 33.0 41.0 38-39 37.50680238636337 40.0 35.0 41.0 33.0 41.0 40-41 37.33006754125751 40.0 35.0 41.0 33.0 41.0 42-43 37.202351436372695 40.0 35.0 41.0 33.0 41.0 44-45 37.06475677359519 40.0 35.0 41.0 33.0 41.0 46-47 36.9003971765772 40.0 35.0 41.0 32.0 41.0 48-49 36.71544481180721 40.0 35.0 41.0 31.5 41.0 50-51 36.44200254400684 39.0 35.0 41.0 31.0 41.0 52-53 35.91147634994017 39.0 35.0 41.0 29.0 41.0 54-55 35.20892384735015 39.0 35.0 41.0 27.0 41.0 56-57 34.66907603937311 39.0 35.0 41.0 23.5 41.0 58-59 34.37710972032444 38.0 35.0 40.5 22.5 41.0 60-61 34.01779695615954 37.5 34.0 40.0 21.5 41.0 62-63 33.71038299338511 37.0 34.0 40.0 20.5 41.0 64-65 33.388230428492875 36.5 34.0 40.0 19.5 41.0 66-67 33.011956242138474 36.0 33.5 39.5 17.5 41.0 68-69 32.682212129579156 35.0 33.0 39.0 14.5 41.0 70-71 32.33781378247562 35.0 33.0 39.0 12.0 41.0 72-73 32.07630132557388 35.0 33.0 37.5 9.0 40.0 74-75 31.815451655610385 35.0 33.0 37.0 7.0 40.0 76-77 31.567473291468136 35.0 33.0 37.0 2.0 39.0 78-79 31.259021902530083 35.0 33.0 36.0 2.0 39.0 80-81 30.949667131573296 35.0 33.0 36.0 2.0 38.0 82-83 30.625691991098336 35.0 32.5 35.5 2.0 37.0 84-85 30.3552107301394 35.0 32.0 35.0 2.0 37.0 86-87 30.20788665717974 35.0 32.0 35.0 2.0 36.5 88-89 30.059841034971832 35.0 32.0 35.0 2.0 36.0 90-91 29.869014799134376 35.0 32.0 35.0 2.0 36.0 92-93 29.719318522399295 35.0 32.0 35.0 2.0 36.0 94-95 29.522769451150587 35.0 32.0 35.0 2.0 35.5 96-97 29.33263314147085 35.0 32.0 35.0 2.0 35.0 98-99 29.180464835831323 35.0 32.0 35.0 2.0 35.0 100 29.027920828996958 35.0 31.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 4.060608523381681 1101 2 4.161803829042221 1101 3 3.532313004864701 1101 4 1.9663692333774208 1101 5 1.1276389924899775 1101 6 0.9017943971974418 1101 7 0.48986630089430605 1101 8 0.5334761865718392 1101 9 0.6358429081972403 1101 10-11 0.43421393591255253 1101 12-13 0.4415951778859579 1101 14-15 0.5433267316389347 1101 16-17 0.5012314619954523 1101 18-19 0.4732028062956175 1101 20-21 0.5334403638163749 1101 22-23 0.5093157966852857 1101 24-25 0.49980086627453346 1101 26-27 0.4560686688409419 1101 28-29 0.46710909798927247 1101 30-31 0.5498729105598557 1101 32-33 0.47187016099415047 1101 34-35 0.42370583603053547 1101 36-37 0.30950847793023684 1101 38-39 0.36702481535603937 1101 40-41 0.3363569449677186 1101 42-43 0.32520416892869974 1101 44-45 0.3339667347728934 1101 46-47 0.2883125239312392 1101 48-49 0.21559992533151728 1101 50-51 0.1865541306625218 1101 52-53 0.2082667168507939 1101 54-55 0.1783882335490503 1101 56-57 0.23538612407057968 1101 58-59 0.22557595560376598 1101 60-61 0.21177678051979143 1101 62-63 0.2038983435276549 1101 64-65 0.18570014907137988 1101 66-67 0.20063388825078476 1101 68-69 0.24566674728031757 1101 70-71 0.23874129941428635 1101 72-73 0.2223961673350061 1101 74-75 0.2675391506056535 1101 76-77 0.2531645656453172 1101 78-79 0.2709227928858944 1101 80-81 0.2088664976514636 1101 82-83 0.21583078468521322 1101 84-85 0.2228575053774975 1101 86-87 0.23306728938796084 1101 88-89 0.2062896097587199 1101 90-91 0.2556267326663715 1101 92-93 0.24785279555592155 1101 94-95 0.2768277206787886 1101 96-97 0.3414496382377301 1101 98-99 0.32128085404911033 1101 100 0.38762535160482514 1102 1 2.8835055341103697 1102 2 3.5178559016838413 1102 3 4.203120327929508 1102 4 2.149779887848254 1102 5 1.1618712791749601 1102 6 0.9290868304923805 1102 7 0.5182779087481677 1102 8 0.5525033679794333 1102 9 0.6680559791251994 1102 10-11 0.4518099339262278 1102 12-13 0.36632903134994876 1102 14-15 0.48725589247885637 1102 16-17 0.5325054833258349 1102 18-19 0.47746621892876107 1102 20-21 0.5335542608726698 1102 22-23 0.47964744605098986 1102 24-25 0.5915441397417638 1102 26-27 0.5221992046253945 1102 28-29 0.5111898149818543 1102 30-31 0.5058613278431849 1102 32-33 0.4657726765993715 1102 34-35 0.47736679183016406 1102 36-37 0.5055448860498828 1102 38-39 0.5236759645701099 1102 40-41 0.44884833323907003 1102 42-43 0.4379101150693785 1102 44-45 0.4650506911200907 1102 46-47 0.5272872818784009 1102 48-49 0.5455721749755611 1102 50-51 0.5205796588236353 1102 52-53 0.4651549675675568 1102 54-55 0.318797531806851 1102 56-57 0.4282843286290401 1102 58-59 0.4222434780741011 1102 60-61 0.435267806767051 1102 62-63 0.37391840192213266 1102 64-65 0.3905804219985072 1102 66-67 0.37475099580807836 1102 68-69 0.38699957114667427 1102 70-71 0.4264685682791267 1102 72-73 0.40925594869001003 1102 74-75 0.47243452732361746 1102 76-77 0.4539100416047539 1102 78-79 0.5240043980841023 1102 80-81 0.4892750157412742 1102 82-83 0.4629347203362464 1102 84-85 0.48747739128528167 1102 86-87 0.4349291736015033 1102 88-89 0.4260898251243077 1102 90-91 0.46150021407007813 1102 92-93 0.5098359377644393 1102 94-95 0.5634640896546408 1102 96-97 0.5650894730083245 1102 98-99 0.5387438453261701 1102 100 0.6142296029647731 1103 1 2.6123998917454543 1103 2 3.284757559329563 1103 3 2.982341656331311 1103 4 1.7099357808350533 1103 5 0.8972050991270635 1103 6 0.7545096013390236 1103 7 0.43799752264927605 1103 8 0.488153378490793 1103 9 0.617385837091895 1103 10-11 0.4368958706232746 1103 12-13 0.40807570656787817 1103 14-15 0.5039899801555876 1103 16-17 0.5360605594775265 1103 18-19 0.4624384297343411 1103 20-21 0.4506377421020673 1103 22-23 0.44115415382803036 1103 24-25 0.4271045320178217 1103 26-27 0.38417177730783436 1103 28-29 0.3673086655217048 1103 30-31 0.387260841420364 1103 32-33 0.38390946942351434 1103 34-35 0.3438823852185493 1103 36-37 0.331952286391747 1103 38-39 0.36628813676971816 1103 40-41 0.3552522321494962 1103 42-43 0.3708787668457987 1103 44-45 0.32864946544592755 1103 46-47 0.35488433273808795 1103 48-49 0.36234634410841693 1103 50-51 0.32585476532076285 1103 52-53 0.3042001590220167 1103 54-55 0.26430308753822374 1103 56-57 0.3242296947244441 1103 58-59 0.3155901112876123 1103 60-61 0.30787101053579846 1103 62-63 0.34443892611339066 1103 64-65 0.3935280563006103 1103 66-67 0.45658473199512883 1103 68-69 0.4965033532872809 1103 70-71 0.502494412160452 1103 72-73 0.41543218848392627 1103 74-75 0.472146652608874 1103 76-77 0.4836562828347404 1103 78-79 0.4220199786793266 1103 80-81 0.460085749998143 1103 82-83 0.43006254930909193 1103 84-85 0.4789937450867008 1103 86-87 0.486640266957874 1103 88-89 0.48940454787566523 1103 90-91 0.5618656771151329 1103 92-93 0.5846960542344242 1103 94-95 0.574669444894429 1103 96-97 0.5338301864286272 1103 98-99 0.5800780292284671 1103 100 0.6008609837328862 1104 1 2.5669135537042074 1104 2 3.3269344497306435 1104 3 1.7689882574531346 1104 4 1.157670905286487 1104 5 0.6708050306641908 1104 6 0.6762184776921316 1104 7 0.3844646167728243 1104 8 0.42716452878151756 1104 9 0.4518765027570524 1104 10-11 0.374410440332845 1104 12-13 0.35483110605909474 1104 14-15 0.458340161569474 1104 16-17 0.53961473962827 1104 18-19 0.37935814148166713 1104 20-21 0.4186241382858782 1104 22-23 0.3788751296492663 1104 24-25 0.4071749294931237 1104 26-27 0.36466192550415144 1104 28-29 0.3812417821286189 1104 30-31 0.29663717972143644 1104 32-33 0.3662984153042359 1104 34-35 0.3399161196265794 1104 36-37 0.29438286507563305 1104 38-39 0.26154717598560495 1104 40-41 0.3452317014641295 1104 42-43 0.31548310713389327 1104 44-45 0.24301367511674954 1104 46-47 0.135170080287061 1104 48-49 0.24040890160369344 1104 50-51 0.33990210027445755 1104 52-53 0.3371682661889466 1104 54-55 0.30842790155014654 1104 56-57 0.38062829099936124 1104 58-59 0.31469873577439245 1104 60-61 0.3343813977053358 1104 62-63 0.3412241304836101 1104 64-65 0.377739499102951 1104 66-67 0.4349502463306365 1104 68-69 0.4528421353967431 1104 70-71 0.3914429047508108 1104 72-73 0.33156303505968054 1104 74-75 0.4221576951541586 1104 76-77 0.44750975321010245 1104 78-79 0.48120201406162266 1104 80-81 0.46425736559655917 1104 82-83 0.4587966398221681 1104 84-85 0.4434954028766356 1104 86-87 0.45297467611038655 1104 88-89 0.42148311692532303 1104 90-91 0.4469232850427254 1104 92-93 0.46565838139428806 1104 94-95 0.43197813813479513 1104 96-97 0.48849257752714337 1104 98-99 0.5052795590865848 1104 100 0.41312956041920756 1105 1 1.502506461684309 1105 2 2.7606987063207313 1105 3 1.6141758833125515 1105 4 1.031188855158419 1105 5 0.5818997882012198 1105 6 0.5390918299678589 1105 7 0.25448663129149907 1105 8 0.3565597521980166 1105 9 0.43242668089377645 1105 10-11 0.2967171610057875 1105 12-13 0.3045533764328425 1105 14-15 0.35692264046743816 1105 16-17 0.4377553983698519 1105 18-19 0.34250479553681856 1105 20-21 0.35117401190158404 1105 22-23 0.2735575592711754 1105 24-25 0.3551550193456734 1105 26-27 0.2625360834954762 1105 28-29 0.2687227473220659 1105 30-31 0.26498713699517396 1105 32-33 0.2636112416504517 1105 34-35 0.2580784967998113 1105 36-37 0.2716349253926822 1105 38-39 0.2023401122823998 1105 40-41 0.09506333561684954 1105 42-43 0.19143087720383534 1105 44-45 0.207256927831871 1105 46-47 0.18109185391651295 1105 48-49 0.07716215219057432 1105 50-51 0.0850321865560133 1105 52-53 0.03284778231455476 1105 54-55 0.08793784074284616 1105 56-57 0.11903341107970533 1105 58-59 0.03208371852776537 1105 60-61 0.005043736297530188 1105 62-63 -0.3201740938197162 1105 64-65 -0.22702889714259555 1105 66-67 -0.12343353843670712 1105 68-69 -0.028420961967796643 1105 70-71 0.002948065816767098 1105 72-73 -0.046250823463836355 1105 74-75 -0.06985517799175867 1105 76-77 -0.1015902229952701 1105 78-79 -0.09340978444990355 1105 80-81 -0.09299011793292777 1105 82-83 -0.14520430783574767 1105 84-85 -0.1458998860379026 1105 86-87 -0.05336948180556078 1105 88-89 -0.0018418515010338865 1105 90-91 0.020312603048441957 1105 92-93 -0.08504497332549832 1105 94-95 -0.07569607857147531 1105 96-97 -0.06860087345861032 1105 98-99 -0.10589333402097623 1105 100 -0.05853773789977268 1106 1 2.0023354213204954 1106 2 2.931272354996082 1106 3 1.1500258085364052 1106 4 0.835710784288473 1106 5 0.5723797265618771 1106 6 0.5213285680156687 1106 7 0.27953292696681586 1106 8 0.40131147315664606 1106 9 0.37528551615030636 1106 10-11 0.28344757951724375 1106 12-13 0.21042759118878962 1106 14-15 0.2120645954173952 1106 16-17 0.31691709173787075 1106 18-19 0.1196134779147755 1106 20-21 0.23463122646964507 1106 22-23 0.23042313747897225 1106 24-25 0.3029241758889185 1106 26-27 0.23538756671699446 1106 28-29 0.23432878068988572 1106 30-31 0.20581261740029788 1106 32-33 0.16094397070118305 1106 34-35 0.1803713486899312 1106 36-37 0.2518962372596718 1106 38-39 0.29443209256882596 1106 40-41 0.23103641890477888 1106 42-43 0.2100407889569098 1106 44-45 0.14449963023720613 1106 46-47 0.17178151927063823 1106 48-49 0.11971175097038866 1106 50-51 0.19994266177905473 1106 52-53 0.2370194522869511 1106 54-55 0.16810643973052208 1106 56-57 0.13611507718208316 1106 58-59 0.15530772316062524 1106 60-61 0.04580776370035977 1106 62-63 0.25364438410288415 1106 64-65 0.03776658944428135 1106 66-67 -0.03765902400905219 1106 68-69 0.13738606183175506 1106 70-71 0.21310527081557495 1106 72-73 0.2422587119014139 1106 74-75 0.24835866729359068 1106 76-77 0.22541658234072415 1106 78-79 0.25412276475638507 1106 80-81 0.21632298936726357 1106 82-83 0.2516567098958298 1106 84-85 0.25020425557847403 1106 86-87 0.2826684797929957 1106 88-89 0.263013814287536 1106 90-91 0.28051698368022926 1106 92-93 0.2632617810854754 1106 94-95 0.29543711515091076 1106 96-97 0.35121495387848256 1106 98-99 0.4114708412507433 1106 100 0.369227325377512 1107 1 1.5566547748082726 1107 2 0.9197422294377802 1107 3 0.5701698580288941 1107 4 0.42814770320539 1107 5 0.24364119885122903 1107 6 0.38032494865331046 1107 7 0.22390920767442424 1107 8 0.24966653863360477 1107 9 0.30397889518911825 1107 10-11 0.18952401967061405 1107 12-13 0.15955518441391092 1107 14-15 0.22170331769449803 1107 16-17 0.24546394480166356 1107 18-19 0.15597138820434964 1107 20-21 0.2082169081198728 1107 22-23 0.1495871365455841 1107 24-25 0.14124176805934496 1107 26-27 0.20308543165934623 1107 28-29 0.21426483206373348 1107 30-31 0.182479124372243 1107 32-33 0.16218109622542443 1107 34-35 0.2060016449151334 1107 36-37 0.19514596433653963 1107 38-39 0.2237317726389776 1107 40-41 0.17640140269879367 1107 42-43 0.024567048506490607 1107 44-45 0.0646993527691535 1107 46-47 0.005648957050269132 1107 48-49 -0.0639252345811201 1107 50-51 -0.02460127925460398 1107 52-53 0.002138359880369478 1107 54-55 -0.11715914047773168 1107 56-57 -0.048116778780809 1107 58-59 -0.07779932336573125 1107 60-61 -0.10108369514002646 1107 62-63 -0.04273282890611796 1107 64-65 -0.1022915673641478 1107 66-67 -0.03334665185210639 1107 68-69 -0.0386282030354792 1107 70-71 0.017106047004766367 1107 72-73 -0.07391790602314785 1107 74-75 0.0055858020005779 1107 76-77 -0.03643430645145784 1107 78-79 0.028717087044491052 1107 80-81 0.032286501371054044 1107 82-83 0.031647468854384186 1107 84-85 0.04513759001692108 1107 86-87 0.02807633068889004 1107 88-89 0.02197721918049922 1107 90-91 0.04307759556723667 1107 92-93 0.053257839115499195 1107 94-95 0.07303662029159241 1107 96-97 0.11889643796824245 1107 98-99 0.14055599454218992 1107 100 0.13978559139162527 1108 1 1.602887586143762 1108 2 -0.526808196370272 1108 3 0.321462900480487 1108 4 0.1992910578998277 1108 5 0.12565142254545236 1108 6 0.14617500976420672 1108 7 0.06807452702796013 1108 8 0.05556995708055723 1108 9 0.08545166828621831 1108 10-11 0.09671770362945864 1108 12-13 0.10262500446913236 1108 14-15 0.12938632622232404 1108 16-17 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-0.4035721157127057 2215 14-15 -0.4911131227971808 2215 16-17 -0.47961198285671713 2215 18-19 -0.6019856966371293 2215 20-21 -0.4985311625189297 2215 22-23 -0.5341438053658933 2215 24-25 -0.5838667779742863 2215 26-27 -0.7183127222806434 2215 28-29 -0.649219625992707 2215 30-31 -0.6490277006666503 2215 32-33 -0.6363255785419994 2215 34-35 -0.6825952439244389 2215 36-37 -0.7239330979694358 2215 38-39 -0.8051159449873921 2215 40-41 -0.8125245864733515 2215 42-43 -0.8422778385885437 2215 44-45 -0.8854313887288399 2215 46-47 -0.8747269906089059 2215 48-49 -0.845441115987569 2215 50-51 -0.8391419980448163 2215 52-53 -0.7891416184828586 2215 54-55 -0.9458253782526 2215 56-57 -1.0145525913714195 2215 58-59 -1.0261108118037683 2215 60-61 -0.9849640296930176 2215 62-63 -0.9341585227986755 2215 64-65 -1.0041873492649955 2215 66-67 -0.9951774910748554 2215 68-69 -1.0034604398022928 2215 70-71 -1.0327551474429555 2215 72-73 -1.091136943507287 2215 74-75 -1.0360745389708121 2215 76-77 -1.0654716017850383 2215 78-79 -1.0345002455923407 2215 80-81 -1.039975730471376 2215 82-83 -1.1417733502932172 2215 84-85 -1.1231706066451785 2215 86-87 -1.174111794909095 2215 88-89 -1.157402392842883 2215 90-91 -1.1967917310204932 2215 92-93 -1.172999218984227 2215 94-95 -1.2367223557492828 2215 96-97 -1.2630604868476318 2215 98-99 -1.2228227495571709 2215 100 -1.34713453670998 2216 1 -3.374826165844105 2216 2 -4.371987765182904 2216 3 -5.838284967656456 2216 4 -3.3087392790246177 2216 5 -1.6110447271336383 2216 6 -0.8915930286468878 2216 7 -0.6416014234915508 2216 8 -0.4532609694701435 2216 9 -0.2927333129636409 2216 10-11 -0.43885388963101946 2216 12-13 -0.4149011225984083 2216 14-15 -0.589997621456881 2216 16-17 -0.5287311031110775 2216 18-19 -0.5025901310356673 2216 20-21 -0.43125549164312815 2216 22-23 -0.5102528003061906 2216 24-25 -0.5095655343208918 2216 26-27 -0.684648548756968 2216 28-29 -0.6417916514354403 2216 30-31 -0.8636017476808178 2216 32-33 -0.9617297500953654 2216 34-35 -0.9542009148972852 2216 36-37 -0.9406635669454815 2216 38-39 -1.0056940483487082 2216 40-41 -1.0773884945767875 2216 42-43 -1.1431152135023126 2216 44-45 -1.223948778643809 2216 46-47 -1.1737936637696365 2216 48-49 -1.2023040281777284 2216 50-51 -1.097098126406351 2216 52-53 -1.0646247913712017 2216 54-55 -1.074658876651256 2216 56-57 -1.011507676054002 2216 58-59 -1.0617087435615318 2216 60-61 -1.0629772165452422 2216 62-63 -1.0265135257039688 2216 64-65 -1.0723501860192073 2216 66-67 -1.0491665492331315 2216 68-69 -0.978248301323358 2216 70-71 -0.9824848225876863 2216 72-73 -1.028879547292327 2216 74-75 -1.1072595947080544 2216 76-77 -1.0742694269969029 2216 78-79 -1.0920036408206428 2216 80-81 -1.003127961388298 2216 82-83 -0.9868127644984774 2216 84-85 -0.9954016744073861 2216 86-87 -1.0466738626288006 2216 88-89 -1.0748405527913754 2216 90-91 -1.0852097022976004 2216 92-93 -1.1706417253559387 2216 94-95 -1.189479841727536 2216 96-97 -1.2337078062754436 2216 98-99 -1.3185883818513169 2216 100 -1.3465901301217365 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4036.0 3 140.0 4 285.0 5 689.0 6 1581.0 7 2371.0 8 3313.0 9 4702.0 10 6508.0 11 9545.0 12 10604.0 13 12602.0 14 13415.0 15 15267.0 16 16196.0 17 20076.0 18 26905.0 19 42464.0 20 72318.0 21 75578.0 22 39056.0 23 28491.0 24 29017.0 25 30672.0 26 33583.0 27 39476.0 28 48640.0 29 59691.0 30 73135.0 31 90347.0 32 113949.0 33 150939.0 34 213101.0 35 386550.0 36 521886.0 37 828312.0 38 925154.0 39 285564.0 40 1252.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 17.307716820626656 39.481803104630316 26.656496038364814 16.55398403637821 2 19.25460648969306 37.66701763056648 24.660772266912883 18.417603612827577 3 20.783633192130296 24.40782873249329 21.307710516546337 33.500827558830075 4 31.99086234279902 26.113120986640425 22.89188442940381 19.004132241156743 5 17.487610382003094 37.38076194823159 23.963818704588412 21.167808965176906 6 34.79434280769166 22.964850578201037 24.515270921777603 17.725535692329693 7 19.62568565023702 23.066072812084577 38.14555195507812 19.16268958260028 8 33.922938776280795 23.10333434810415 24.366322824555567 18.607404051059493 9 33.45430345423266 25.068001444278465 24.23022553871351 17.247469562775375 10-11 17.48399376033945 32.80522772165072 31.6128484144796 18.09793010353022 12-13 17.267540785527007 25.287085743414018 31.4422961195636 26.003077351495367 14-15 17.650958958420357 40.40849245175709 23.42737662864816 18.513171961174397 16-17 25.654114187675965 24.676830894343478 23.600395052638287 26.068659865342276 18-19 18.16341822009199 32.60476328700786 23.723795903629814 25.508022589270336 20-21 25.843215549120806 32.459863454327056 24.001689711403902 17.69523128514824 22-23 25.168935741408077 24.893791254563517 31.96378212162618 17.97349088240222 24-25 17.59862206754531 32.46704377227063 24.15276906887609 25.781565091307968 26-27 18.05836545617623 28.300245241636112 35.841351801445725 17.800037500741933 28-29 25.73932512039285 24.584542611020304 31.907313909686604 17.768818358900244 30-31 23.37639335820641 26.073414005047223 32.32157988209458 18.228612754651785 32-33 19.694894277736623 27.369832863170778 32.939648403528096 19.995624455564506 34-35 19.97148715682984 27.052200145478988 33.329273893696495 19.647038803994676 36-37 20.183136018461344 26.584506647524996 33.98348688873976 19.2488704452739 38-39 21.2053485296594 26.530430904912944 33.106326176385174 19.157894389042486 40-41 19.35681970357129 27.579330754331032 33.640013698245404 19.423835843852274 42-43 19.499906595513256 26.890461378041298 33.49339742977759 20.116234596667862 44-45 19.633092416415646 28.290040587612907 32.592334636218446 19.484532359753004 46-47 20.43384328852712 26.775993299779522 32.43844432058482 20.35171909110853 48-49 19.641223248793903 27.83736357163704 32.46465440154328 20.05675877802578 50-51 20.672366299084416 27.89927209670177 32.078927759750705 19.349433844463107 52-53 20.581250211678366 27.402326733216576 32.3131586589994 19.70326439610566 54-55 20.11790197405623 28.684327536059495 30.38898187561303 20.808788614271243 56-57 20.372610209043945 29.276855257062202 29.84510211002768 20.50543242386618 58-59 22.004226771020953 29.008596920421226 28.587866932119248 20.39930937643858 60-61 21.06892576236986 29.842276118227236 28.59755715450525 20.491240964897653 62-63 20.224749333438126 29.76498951403441 28.45813526476665 21.552125887760813 64-65 21.012143403110215 29.707750425162494 28.77215102187101 20.50795514985628 66-67 21.274640318991096 28.61742322102505 29.602133215765413 20.50580324421844 68-69 21.842301305991835 28.693032267520106 29.297935427670346 20.166730998817712 70-71 19.971120188350085 29.50302745953947 30.023805725268666 20.502046626841775 72-73 19.854979551075935 28.946824065384636 29.9774182140388 21.22077816950063 74-75 20.108415659677867 30.293526330309632 28.89244644529787 20.705611564714633 76-77 20.99446985750201 28.922600424843704 28.56692775131846 21.51600196633582 78-79 20.600619586670035 29.883197977222036 28.12322633462825 21.39295610147968 80-81 21.364633722552718 29.920510038461746 28.117014792932572 20.597841446052968 82-83 21.034570402796273 29.50837468580031 28.743121000303727 20.713933911099687 84-85 20.567516228858587 30.040456803400424 27.918451091823943 21.47357587591705 86-87 20.71575983773011 30.20758085470166 28.255610476744504 20.821048830823727 88-89 22.328370762552616 29.436710909297513 27.622630493302008 20.61228783484786 90-91 21.48477692840677 30.152046640096813 27.691920297995175 20.671256133501238 92-93 20.851593862314715 30.22994903355139 27.3672235692892 21.551233534844695 94-95 21.25175830792752 30.60143128418752 27.324538833929 20.822271573955955 96-97 21.41599367435806 29.578653578003173 27.96730697546926 21.03804577216951 98-99 22.23112864232331 29.75421899204865 26.991837665212586 21.022814700415456 100 20.772473504395737 29.76329122497493 28.2490847573784 21.21515051325093 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 107082.0 1 62204.0 2 12610.0 3 6533.0 4 4719.0 5 4047.5 6 3958.5 7 4160.0 8 3884.5 9 3498.5 10 3570.5 11 5311.5 12 28342.0 13 30670.5 14 10385.0 15 9766.0 16 10935.0 17 12048.5 18 13315.0 19 14909.0 20 16738.0 21 18928.5 22 21440.5 23 24788.5 24 28654.5 25 33141.0 26 39507.5 27 46338.0 28 52172.0 29 57269.5 30 62686.0 31 68945.5 32 75731.5 33 82467.5 34 89531.0 35 95688.0 36 99991.5 37 103954.5 38 106079.0 39 106819.5 40 107470.0 41 108098.5 42 108813.5 43 110125.5 44 112961.5 45 116970.0 46 125483.0 47 138365.0 48 146039.0 49 158479.0 50 202007.0 51 196766.0 52 139652.0 53 116804.5 54 111015.5 55 107331.5 56 100163.0 57 90122.5 58 82787.0 59 71101.5 60 57083.5 61 46397.0 62 37396.5 63 29137.0 64 21953.5 65 16479.0 66 12282.5 67 9454.5 68 7324.5 69 5398.5 70 3947.5 71 3015.0 72 2410.0 73 1747.0 74 1154.5 75 733.5 76 510.5 77 368.0 78 231.5 79 154.5 80 100.5 81 73.5 82 52.5 83 37.5 84 30.0 85 25.5 86 18.0 87 10.5 88 9.5 89 8.0 90 8.5 91 8.0 92 4.0 93 3.5 94 3.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02426010227945844 2 0.011964855890744582 3 0.005876230999596452 4 0.0 5 0.004554669007719338 6 0.001014770815191355 7 2.1239389155167896E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011091681003254346 26-27 0.0037876910660049417 28-29 7.551782810726363E-4 30-31 7.197792991473566E-4 32-33 0.005262648646224935 34-35 0.008778947517469398 36-37 0.0054632428771348536 38-39 0.005793633375104132 40-41 0.008755348196185878 42-43 0.010891086772344427 44-45 0.009510526477258514 46-47 0.01261383722604138 48-49 0.01672011912937384 50-51 0.06078005196570546 52-53 0.01037190170410699 54-55 0.005805433035745892 56-57 0.0039410866543478215 58-59 0.008719949214260598 60-61 0.015823344920600085 62-63 0.013133022294278818 64-65 0.011811460302401704 66-67 0.010065110527421232 68-69 0.006737606226444928 70-71 0.004578268329002858 72-73 0.0034337012467521434 74-75 0.002525127377336628 76-77 0.00205314095166623 78-79 0.0016283531685628721 80-81 0.0019469440058903908 82-83 0.0013451613131606336 84-85 0.0012035653854595143 86-87 5.899830320879972E-4 88-89 0.0013333616525188737 90-91 6.13582353371517E-4 92-93 3.4219015861103834E-4 94-95 6.253820140132769E-4 96-97 0.001262563688668314 98-99 0.0033511036222598238 100 2.1239389155167896E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4237410.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.63953815757695 #Duplication Level Percentage of deduplicated Percentage of total 1 81.53569542812359 46.99679827833903 2 11.692300112864055 13.47877557010538 3 3.611531244634102 6.2450097894710614 4 1.35090714559328 3.114626558630689 5 0.5864025311982021 1.6899985536349236 6 0.33707283623342293 1.1657233563575442 7 0.19190482662301978 0.7742913903732518 8 0.12362335753259525 0.5700474586934241 9 0.08622415255423979 0.44729282981293567 >10 0.36434117344940936 4.194596873023053 >50 0.0879419319505325 3.5411970544481615 >100 0.026794172795182772 2.831796967068266 >500 0.0015905610610463686 0.6534846648271523 >1k 0.003262689233239845 3.9855769799633185 >5k 2.0391807707748993E-4 0.8001478618891228 >10k+ 2.0391807707748993E-4 9.510635813362695 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 220115 5.19456460432198 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 96210 2.270490700687448 No Hit AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG 43656 1.0302519699533441 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26210 0.6185382108410562 No Hit TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 18388 0.43394431976136366 No Hit AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 8448 0.199367066203176 No Hit ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 7925 0.1870246211718951 No Hit TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 6352 0.14990288879291833 No Hit ACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 6050 0.14277589376529531 No Hit AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA 5263 0.12420322791516515 No Hit AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG 4979 0.11750102067064551 No Hit CGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT 4238 0.10001392359955728 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.663837108044773E-4 0.0 0.0 0.0 2.3599321283519885E-5 2 5.899830320879972E-4 0.0 0.0 0.0 2.3599321283519885E-5 3 6.843803172220767E-4 0.0 0.0 0.0 2.3599321283519885E-5 4 6.843803172220767E-4 0.0 0.0 0.0 2.3599321283519885E-5 5 6.843803172220767E-4 0.0 0.0 0.0 7.079796385055966E-5 6 6.843803172220767E-4 0.0 0.0 0.0 1.1799660641759943E-4 7 6.843803172220767E-4 0.0 0.0 0.0 1.651952489846392E-4 8 6.843803172220767E-4 0.0 0.0 0.0 1.651952489846392E-4 9 6.843803172220767E-4 0.0 0.0 0.0 1.651952489846392E-4 10-11 6.961799778638367E-4 0.0 0.0 0.0 1.651952489846392E-4 12-13 7.079796385055966E-4 0.0 0.0 0.0 1.7699490962639914E-4 14-15 7.551782810726363E-4 0.0 0.0 2.3599321283519885E-5 1.8879457026815908E-4 16-17 7.787776023561562E-4 0.0 0.0 2.3599321283519885E-5 1.8879457026815908E-4 18-19 8.141765842814361E-4 0.0 0.0 2.3599321283519885E-5 1.8879457026815908E-4 20-21 8.613752268484759E-4 0.0 0.0 2.3599321283519885E-5 1.8879457026815908E-4 22-23 9.321731906990355E-4 0.0 0.0 2.3599321283519885E-5 1.8879457026815908E-4 24-25 0.0010029711545495952 0.0 0.0 2.3599321283519885E-5 1.8879457026815908E-4 26-27 0.0010265704758331152 0.0 0.0 2.3599321283519885E-5 1.8879457026815908E-4 28-29 0.0010737691184001547 0.0 0.0 2.3599321283519885E-5 1.8879457026815908E-4 30-31 0.0011563667428924744 0.0 0.0 2.3599321283519885E-5 1.8879457026815908E-4 32-33 0.001427758937652953 0.0 0.0 2.3599321283519885E-5 1.8879457026815908E-4 34-35 0.0015339558834287926 0.0 0.0 2.3599321283519885E-5 1.8879457026815908E-4 36-37 0.0018053480781892713 0.0 0.0 2.3599321283519885E-5 1.8879457026815908E-4 38-39 0.002277334503859669 0.0 0.0 2.3599321283519885E-5 1.8879457026815908E-4 40-41 0.0029381154997982256 0.0 0.0 2.3599321283519885E-5 2.1239389155167898E-4 42-43 0.0038230900479302218 0.0 0.0 2.3599321283519885E-5 2.1239389155167898E-4 44-45 0.004861460184405097 0.0 0.0 2.3599321283519885E-5 2.1239389155167898E-4 46-47 0.006017826927297571 0.0 0.0 2.3599321283519885E-5 2.1239389155167898E-4 48-49 0.007598981453293402 0.0 0.0 2.3599321283519885E-5 2.1239389155167898E-4 50-51 0.009604923762392595 0.0 0.0 3.539898192527983E-5 2.1239389155167898E-4 52-53 0.0121064518184457 0.0 0.0 4.719864256703977E-5 2.1239389155167898E-4 54-55 0.015245161549153848 0.0 0.0 4.719864256703977E-5 2.1239389155167898E-4 56-57 0.019611035986605026 0.0 0.0 7.079796385055966E-5 2.1239389155167898E-4 58-59 0.02469668972320356 0.0 0.0 7.079796385055966E-5 2.1239389155167898E-4 60-61 0.031847284072110085 0.0 0.0 7.079796385055966E-5 2.1239389155167898E-4 62-63 0.04089762378433996 0.0 0.0 7.079796385055966E-5 2.1239389155167898E-4 64-65 0.0537002555806495 0.0 0.0 7.079796385055966E-5 2.1239389155167898E-4 66-67 0.06925220830648911 0.0 0.0 7.079796385055966E-5 2.1239389155167898E-4 68-69 0.09022020526689653 0.0 0.0 7.079796385055966E-5 2.1239389155167898E-4 70-71 0.1194479646765359 0.0 0.0 7.079796385055966E-5 2.1239389155167898E-4 72-73 0.15911842375413282 0.0 0.0 7.079796385055966E-5 2.1239389155167898E-4 74-75 0.20768582695561677 0.0 0.0 7.079796385055966E-5 2.1239389155167898E-4 76-77 0.27067241546133136 0.0 0.0 7.079796385055966E-5 2.1239389155167898E-4 78-79 0.34845577841181286 0.0 0.0 7.079796385055966E-5 2.1239389155167898E-4 80-81 0.44825730811981845 0.0 0.0 7.079796385055966E-5 2.1239389155167898E-4 82-83 0.5718941523241792 0.0 0.0 7.079796385055966E-5 2.5959253411871877E-4 84-85 0.7252307423638495 0.0 0.0 7.079796385055966E-5 3.539898192527983E-4 86-87 0.910768606294883 0.0 0.0 7.079796385055966E-5 0.0023599321283519887 88 1.0702764188501939 0.0 0.0 7.079796385055966E-5 0.002407130770919028 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 4940 0.0 86.70769 1 ATCGAGT 2010 0.0 83.7439 1 TCGAGTG 2740 0.0 70.33663 2 AGTGGTA 110250 0.0 55.493843 5 GTGGTAT 113290 0.0 54.02835 6 TGGTATC 113765 0.0 53.938446 7 GGTATCA 113930 0.0 53.888565 8 GTATCAA 114545 0.0 53.689487 9 GAGTGGT 115470 0.0 52.87528 4 ACGAGTG 108195 0.0 52.76411 2 GACGAGT 1435 0.0 52.75215 1 CGAGTGG 111960 0.0 52.68918 3 AACGAGT 100985 0.0 51.79291 1 ATGAGTG 4315 0.0 50.763718 2 AATGAGT 4075 0.0 48.691265 1 TGAGTGG 5140 0.0 46.722836 3 AGTACTT 39845 0.0 39.67554 20-21 GAGTACT 39025 0.0 39.383286 20-21 GTACTTT 39555 0.0 38.297195 22-23 CATGGGG 20955 0.0 37.832767 24-25 >>END_MODULE