FastQCFastQC Report
Thu 26 May 2016
SRR2132632_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132632_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4505673
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2786286.183937449521969No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA581041.2895742766951797No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG545991.2117834561007867No Hit
TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT234950.5214537317732556No Hit
AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT106360.23605796514749294No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA103940.2306869584188644No Hit
ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT97370.21610534097791828No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91590.20327706870871456No Hit
AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG72030.15986513002608044No Hit
ACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT49330.10948419914183741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT62300.089.887931
ATCGAGT25950.084.435721
TCGAGTG31450.076.8219152
AGTGGTA1295500.059.2530985
GTGGTAT1318050.058.167746
GGTATCA1323650.058.080768
TGGTATC1324750.058.0077067
GTATCAA1328950.057.9410679
ATGAGTG47600.057.3735662
GAGTGGT1358700.056.4554334
CGAGTGG1322250.056.0938723
ACGAGTG1283500.055.940722
AACGAGT1200400.054.6379971
TGAGTGG54250.053.627233
AATGAGT48400.051.8768461
GACGAGT17850.050.0487331
GAGTACT462850.041.32450520-21
AGTACTT472400.041.00639720-21
ACGATTG7800.040.978592
GTACTTT469300.040.0808322-23