##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132632_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4505673
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.58465472305691	31.0	23.0	33.0	16.0	34.0
2	27.904141956151722	31.0	25.0	34.0	16.0	34.0
3	28.081439997975885	31.0	25.0	34.0	16.0	34.0
4	33.914838471411485	35.0	33.0	37.0	28.0	37.0
5	34.93838500929828	35.0	35.0	37.0	32.0	37.0
6	35.35104611453161	37.0	35.0	37.0	33.0	37.0
7	35.598570291275024	37.0	35.0	37.0	33.0	37.0
8	35.71350184534031	37.0	35.0	37.0	35.0	37.0
9	37.51567457292174	39.0	37.0	39.0	35.0	39.0
10-11	37.51922254455661	39.0	37.0	39.0	34.5	39.0
12-13	37.711172670542226	39.0	37.0	39.0	35.0	39.0
14-15	39.18303736201007	41.0	39.0	41.0	36.0	41.0
16-17	39.086600603283905	40.5	39.0	41.0	36.0	41.0
18-19	39.06863858074033	40.0	38.5	41.0	35.5	41.0
20-21	39.011218079962745	40.0	39.0	41.0	35.0	41.0
22-23	38.858769489041926	40.0	38.5	41.0	35.0	41.0
24-25	38.73790841013096	40.0	38.0	41.0	34.5	41.0
26-27	38.823017116421894	40.0	38.0	41.0	35.0	41.0
28-29	38.77566325829682	40.0	38.0	41.0	35.0	41.0
30-31	38.65081109969587	40.0	38.0	41.0	35.0	41.0
32-33	38.53138110555294	40.0	38.0	41.0	34.5	41.0
34-35	38.4415433610029	40.0	38.0	41.0	34.0	41.0
36-37	38.10579751349022	40.0	37.5	41.0	34.0	41.0
38-39	37.767528291556005	40.0	36.0	41.0	33.0	41.0
40-41	37.58965319942215	40.0	35.0	41.0	33.0	41.0
42-43	37.48255066446234	40.0	35.0	41.0	33.0	41.0
44-45	37.365673119198846	40.0	35.0	41.0	33.0	41.0
46-47	37.212668784441306	40.0	35.0	41.0	33.0	41.0
48-49	37.03550646041113	40.0	35.0	41.0	32.5	41.0
50-51	36.758167536791944	40.0	35.0	41.0	31.5	41.0
52-53	36.18342786971003	39.0	35.0	41.0	30.0	41.0
54-55	35.38860365588005	39.0	35.0	41.0	27.0	41.0
56-57	34.79187504286263	39.0	35.0	41.0	23.5	41.0
58-59	34.48578092551324	39.0	35.0	41.0	22.0	41.0
60-61	34.117810036369704	38.0	34.5	40.5	20.0	41.0
62-63	33.813813053011174	37.5	34.0	40.0	18.0	41.0
64-65	33.489657260968556	37.0	34.0	40.0	16.5	41.0
66-67	33.10260620333522	36.5	33.5	40.0	12.0	41.0
68-69	32.78185123509851	36.0	33.0	39.0	9.0	41.0
70-71	32.42418191466625	35.5	33.0	39.0	7.0	41.0
72-73	32.16968708115303	35.0	33.0	38.5	6.0	40.5
74-75	31.89923281161327	35.0	33.0	37.5	2.0	40.0
76-77	31.63364540657966	35.0	33.0	37.0	2.0	39.5
78-79	31.308006928154796	35.0	33.0	36.5	2.0	39.0
80-81	30.979710356255325	35.0	33.0	36.0	2.0	39.0
82-83	30.632173151491465	35.0	32.5	36.0	2.0	37.5
84-85	30.337893362434425	35.0	32.0	35.5	2.0	37.0
86-87	30.18348013715154	35.0	32.0	35.0	2.0	37.0
88-89	30.032283634431526	35.0	32.0	35.0	2.0	36.0
90-91	29.820765177588342	35.0	32.0	35.0	2.0	36.0
92-93	29.65976170485519	35.0	32.0	35.0	2.0	36.0
94-95	29.45235672895037	35.0	32.0	35.0	2.0	36.0
96-97	29.255710967040883	35.0	32.0	35.0	2.0	35.5
98-99	29.101736965820646	35.0	32.0	35.0	2.0	35.0
100	28.932551252609766	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	4.02765744224191
1101	2	4.097337416413616
1101	3	3.519980664233124
1101	4	1.9583858201157796
1101	5	1.1393802089238392
1101	6	0.8993512665131504
1101	7	0.5390202243563209
1101	8	0.5817847864322658
1101	9	0.7237492545648934
1101	10-11	0.5230498305738323
1101	12-13	0.47972827588959177
1101	14-15	0.605865738569598
1101	16-17	0.5975099997700752
1101	18-19	0.5531405111214696
1101	20-21	0.5394771971775469
1101	22-23	0.5263018828220041
1101	24-25	0.5698645422129118
1101	26-27	0.48555157325671416
1101	28-29	0.48251984891339106
1101	30-31	0.5151913381480995
1101	32-33	0.5166250981844911
1101	34-35	0.5654488421133763
1101	36-37	0.4954125230753377
1101	38-39	0.4665413346974532
1101	40-41	0.4626126815180811
1101	42-43	0.5145127836333998
1101	44-45	0.49012469197665354
1101	46-47	0.4739955558309248
1101	48-49	0.4859754893902135
1101	50-51	0.5918900731372005
1101	52-53	0.5427608100160697
1101	54-55	0.47612261460088234
1101	56-57	0.41039171657509144
1101	58-59	0.3809195373465286
1101	60-61	0.3521796775813897
1101	62-63	0.3974971302928054
1101	64-65	0.4103336797639088
1101	66-67	0.3540207792801837
1101	68-69	0.4280696012481755
1101	70-71	0.4199091762658469
1101	72-73	0.39906119333726053
1101	74-75	0.39391015164797594
1101	76-77	0.3894718658695808
1101	78-79	0.42831763134757495
1101	80-81	0.39577986967931267
1101	82-83	0.3917398642632719
1101	84-85	0.3480822544433657
1101	86-87	0.3734102503499983
1101	88-89	0.35084547401521604
1101	90-91	0.3336911070837374
1101	92-93	0.3629960962404546
1101	94-95	0.2910912655564921
1101	96-97	0.33542572183137054
1101	98-99	0.3217903193757685
1101	100	0.2738104501049605
1102	1	2.8712260767348177
1102	2	3.5028804548031047
1102	3	4.225538515239325
1102	4	2.1170103577025685
1102	5	1.1175267477090642
1102	6	0.8904928672681791
1102	7	0.48134479556422605
1102	8	0.5319513444403228
1102	9	0.6471688040419039
1102	10-11	0.4405780179435297
1102	12-13	0.36811543412878933
1102	14-15	0.5284374213172427
1102	16-17	0.5582288276458272
1102	18-19	0.41791469814300086
1102	20-21	0.470744874672242
1102	22-23	0.4104884482617521
1102	24-25	0.4977660648418194
1102	26-27	0.46656731958413644
1102	28-29	0.4540749267913995
1102	30-31	0.45744355557798855
1102	32-33	0.4958683560692805
1102	34-35	0.4605774297921954
1102	36-37	0.43219427697759016
1102	38-39	0.45176279150561527
1102	40-41	0.45188301234629336
1102	42-43	0.46133046406769296
1102	44-45	0.4370728630483782
1102	46-47	0.4641612454708195
1102	48-49	0.44811433052772287
1102	50-51	0.46317012272876923
1102	52-53	0.39094707715641164
1102	54-55	0.3260256795761052
1102	56-57	0.3792413629474112
1102	58-59	0.31558542686258306
1102	60-61	0.26675170800384507
1102	62-63	0.22214662848982414
1102	64-65	0.21933405577019016
1102	66-67	0.24603968980853352
1102	68-69	0.24077503521035482
1102	70-71	0.33896535997243404
1102	72-73	0.32623713111345864
1102	74-75	0.23761170255923858
1102	76-77	0.19936798963132674
1102	78-79	0.22441513529474122
1102	80-81	0.18976914482076168
1102	82-83	0.18401452170206412
1102	84-85	0.17911966289214476
1102	86-87	0.19342208529503324
1102	88-89	0.17879524204914787
1102	90-91	0.19103342804800505
1102	92-93	0.22289939703843586
1102	94-95	0.2785665696736821
1102	96-97	0.26063280573399794
1102	98-99	0.2446844569858797
1102	100	0.2638410431942724
1103	1	2.6847046917029225
1103	2	3.2963828315772403
1103	3	3.0190642510224563
1103	4	1.6457954270747024
1103	5	0.8558391299294144
1103	6	0.7205811258934887
1103	7	0.35082877735015217
1103	8	0.42817418866182777
1103	9	0.5152453502065981
1103	10-11	0.3547980832678732
1103	12-13	0.36948008513965647
1103	14-15	0.4053938213144974
1103	16-17	0.4259735088890153
1103	18-19	0.3893290857352767
1103	20-21	0.4123436803327536
1103	22-23	0.37743391137382076
1103	24-25	0.3427737386082512
1103	26-27	0.32456945312078034
1103	28-29	0.3886442803228789
1103	30-31	0.39183356181731455
1103	32-33	0.36162958382040955
1103	34-35	0.3231094505973644
1103	36-37	0.3434757850062411
1103	38-39	0.3582186924429749
1103	40-41	0.3058466730116436
1103	42-43	0.41799347652153784
1103	44-45	0.41874871641826417
1103	46-47	0.3840969745961331
1103	48-49	0.37344402905707597
1103	50-51	0.4141060634935343
1103	52-53	0.28010105469365243
1103	54-55	0.32862598393534626
1103	56-57	0.3254658660408296
1103	58-59	0.319576024831278
1103	60-61	0.3629776365802826
1103	62-63	0.33450972905401954
1103	64-65	0.42530264069972645
1103	66-67	0.5077088276142376
1103	68-69	0.476875796116488
1103	70-71	0.47290545479793167
1103	72-73	0.48379990402379747
1103	74-75	0.521801319284581
1103	76-77	0.4876231100880659
1103	78-79	0.45588842823153897
1103	80-81	0.4541298903300728
1103	82-83	0.37566096585830877
1103	84-85	0.3748993639777112
1103	86-87	0.3925362798847516
1103	88-89	0.399579846816426
1103	90-91	0.43409807087617835
1103	92-93	0.4002470694902449
1103	94-95	0.43602446848031917
1103	96-97	0.4200070184866007
1103	98-99	0.4381878417585945
1103	100	0.396366620252838
1104	1	2.5412525940535247
1104	2	3.353466105734526
1104	3	1.5911775296806496
1104	4	1.098630603900716
1104	5	0.6234300988617605
1104	6	0.5824210728643067
1104	7	0.3562488377689803
1104	8	0.40253138314771775
1104	9	0.4460479426222932
1104	10-11	0.3480327495949709
1104	12-13	0.3425360217785425
1104	14-15	0.38300250934131697
1104	16-17	0.4658331113129677
1104	18-19	0.35647334018631227
1104	20-21	0.39077146196382273
1104	22-23	0.37002513669690984
1104	24-25	0.35494342003766377
1104	26-27	0.34650398054195364
1104	28-29	0.39329325306193397
1104	30-31	0.323416180671785
1104	32-33	0.26827594819766887
1104	34-35	0.31637177121014304
1104	36-37	0.29857399423361386
1104	38-39	0.32444149651810505
1104	40-41	0.3311511425068687
1104	42-43	0.3575325585916005
1104	44-45	0.26118179953152776
1104	46-47	0.2626556332267995
1104	48-49	0.3142390566489084
1104	50-51	0.3847871833620147
1104	52-53	0.42597561589137456
1104	54-55	0.3932921490205743
1104	56-57	0.4575982923987141
1104	58-59	0.3587728322417689
1104	60-61	0.37279242389747935
1104	62-63	0.3878277006245412
1104	64-65	0.33894018208289367
1104	66-67	0.34690511226694554
1104	68-69	0.36701835814918127
1104	70-71	0.32242776192994427
1104	72-73	0.25318234591478017
1104	74-75	0.3060686904159091
1104	76-77	0.34437509747547423
1104	78-79	0.3369781423656377
1104	80-81	0.3729371969036066
1104	82-83	0.3543638038783783
1104	84-85	0.39003030861658416
1104	86-87	0.3898384207534491
1104	88-89	0.4005893080538776
1104	90-91	0.3909330929499255
1104	92-93	0.3784894616585852
1104	94-95	0.37185159120513234
1104	96-97	0.39844635266558015
1104	98-99	0.4140949831366818
1104	100	0.3866710995248326
1105	1	1.326445725377031
1105	2	2.6403302650585836
1105	3	1.5219256636083713
1105	4	0.9243838633668346
1105	5	0.567315137864874
1105	6	0.5425636659874868
1105	7	0.19996807182713638
1105	8	0.3122329765853493
1105	9	0.43549452098759645
1105	10-11	0.29345718125017584
1105	12-13	0.23504015483369756
1105	14-15	0.2992820518173218
1105	16-17	0.3601607406654921
1105	18-19	0.2806285087339617
1105	20-21	0.30212291411923786
1105	22-23	0.24793325702405866
1105	24-25	0.28744688319383727
1105	26-27	0.21813959292597218
1105	28-29	0.24996543706360086
1105	30-31	0.24485679428501328
1105	32-33	0.22536008446343203
1105	34-35	0.21382164224964129
1105	36-37	0.24446074719683253
1105	38-39	0.20065967498085513
1105	40-41	0.15315356118645695
1105	42-43	0.2805725708705751
1105	44-45	0.23912429719113248
1105	46-47	0.23481096337323493
1105	48-49	0.18975732322731886
1105	50-51	0.20250516673102936
1105	52-53	0.04275875384146133
1105	54-55	0.11510139107043216
1105	56-57	0.14723617974861725
1105	58-59	0.105113138077364
1105	60-61	0.06937632882452505
1105	62-63	-0.20934503482705225
1105	64-65	-0.0501263661096516
1105	66-67	0.05088657359400628
1105	68-69	0.12495644349716883
1105	70-71	0.13347075389428653
1105	72-73	0.06651810969280803
1105	74-75	0.06598976381171084
1105	76-77	0.011467916312671633
1105	78-79	0.05410380920299218
1105	80-81	0.06644209889833519
1105	82-83	0.12190190176173843
1105	84-85	0.059332874131854396
1105	86-87	0.07514408363154956
1105	88-89	0.019465415400265584
1105	90-91	0.045112510812064244
1105	92-93	0.051008620551936446
1105	94-95	0.06428435805769084
1105	96-97	0.034685703576592175
1105	98-99	0.0657261337481323
1105	100	0.1016061709604763
1106	1	1.7992534818007222
1106	2	2.8179268644539874
1106	3	1.1542801844221984
1106	4	0.7793770348202145
1106	5	0.5140373655577832
1106	6	0.49869173871773853
1106	7	0.238002596846961
1106	8	0.3356005308215799
1106	9	0.3344317946247344
1106	10-11	0.25085209706722367
1106	12-13	0.16636432556433078
1106	14-15	0.1683336614311699
1106	16-17	0.2790002862385208
1106	18-19	0.09620493858962931
1106	20-21	0.20456403951131108
1106	22-23	0.21601078895560022
1106	24-25	0.26783599974472594
1106	26-27	0.2179031137112588
1106	28-29	0.22186481191735652
1106	30-31	0.25154065620470334
1106	32-33	0.24329909630651514
1106	34-35	0.25812767286752347
1106	36-37	0.2604194696858855
1106	38-39	0.28476569914161587
1106	40-41	0.1892700043791109
1106	42-43	0.20061446698988306
1106	44-45	0.16247176103576066
1106	46-47	0.11961356606682472
1106	48-49	0.06921796781666956
1106	50-51	0.22584205056848106
1106	52-53	0.2575566559073934
1106	54-55	0.13133231581092275
1106	56-57	0.13354799259354877
1106	58-59	0.11757766102432754
1106	60-61	-0.0516179018902676
1106	62-63	0.07633717205331436
1106	64-65	-0.059041829488457154
1106	66-67	-0.22185681241919752
1106	68-69	0.01202612445666773
1106	70-71	0.15659741647563408
1106	72-73	0.09302373621390814
1106	74-75	0.18290972653998594
1106	76-77	0.0950988447682839
1106	78-79	0.10249320846521925
1106	80-81	0.14175729141635074
1106	82-83	0.14192049271161622
1106	84-85	0.06739109297040713
1106	86-87	0.05317252499951053
1106	88-89	0.06078164354162041
1106	90-91	0.0796862349160854
1106	92-93	0.10379330267137377
1106	94-95	0.01761881446210012
1106	96-97	0.06977285645962894
1106	98-99	0.14741827736494528
1106	100	0.17372638253462114
1107	1	1.5734970322399526
1107	2	0.969152039803717
1107	3	0.5090181040820134
1107	4	0.3787274949596764
1107	5	0.21210574460805987
1107	6	0.3571811213945253
1107	7	0.17528001187434228
1107	8	0.23709561688045966
1107	9	0.2841598373972474
1107	10-11	0.18958908340330538
1107	12-13	0.1692400318741889
1107	14-15	0.22219426513181162
1107	16-17	0.25261349510827813
1107	18-19	0.18264959467779107
1107	20-21	0.2497426720221938
1107	22-23	0.1914341224109819
1107	24-25	0.20369512187745187
1107	26-27	0.23876931721509465
1107	28-29	0.27066021312613486
1107	30-31	0.22895057424512544
1107	32-33	0.14672118124649103
1107	34-35	0.17182655150077153
1107	36-37	0.24262819478709474
1107	38-39	0.26296352674151535
1107	40-41	0.29437869886608325
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2216	76-77	-1.069322084654388
2216	78-79	-1.0819277578909627
2216	80-81	-0.9893758649849289
2216	82-83	-1.0247729731955957
2216	84-85	-1.009367763724935
2216	86-87	-1.007270114712668
2216	88-89	-1.0468020785810772
2216	90-91	-1.0791880692526483
2216	92-93	-1.1737808748307899
2216	94-95	-1.137266737423868
2216	96-97	-1.124751821130026
2216	98-99	-1.2420836191710443
2216	100	-1.2314145203636286
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	3970.0
3	104.0
4	204.0
5	395.0
6	821.0
7	1361.0
8	2060.0
9	3031.0
10	4761.0
11	7991.0
12	8687.0
13	11157.0
14	11734.0
15	13913.0
16	15350.0
17	20361.0
18	29046.0
19	49841.0
20	88572.0
21	93876.0
22	45927.0
23	31239.0
24	30106.0
25	32208.0
26	34886.0
27	40367.0
28	49777.0
29	60959.0
30	74524.0
31	91761.0
32	114905.0
33	151680.0
34	212447.0
35	355720.0
36	530445.0
37	863066.0
38	1060787.0
39	355587.0
40	2047.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.036501270078496	41.498799186403836	25.64208783943686	15.822611704080813
2	18.5759555064063	40.21108974765921	23.910955988082836	17.30199875785166
3	19.683480274281877	23.752496427650318	21.252561904081247	35.311461393986555
4	34.61500645963434	25.02394203929136	22.15986379837152	18.201187702702793
5	17.123631845544203	40.43398301018173	23.185992655235065	19.256392489039005
6	36.573237361275936	22.79381492035386	23.501154225936205	17.131793492434
7	18.325134763710743	22.65785908580844	40.7665329682817	18.250473182199116
8	35.87108962412496	22.874318664492517	23.46899120286803	17.785600508514488
9	35.343155173489066	24.118993988245485	23.65380266166675	16.8840481765987
10-11	16.875803015442976	33.37341613561392	32.309535112734544	17.44124573620855
12-13	16.723850132932416	24.319019600401536	32.2228554979467	26.734274768719345
14-15	16.96638881694255	42.346781490800595	22.942909971495933	17.743919720760918
16-17	26.368535843590955	23.83971273547814	22.994456100120892	26.79729532081001
18-19	17.414002303318505	33.1235533515193	23.144533569124967	26.31791077603723
20-21	26.508115435807262	33.00373773240979	23.37124775810406	17.116899073678894
22-23	26.035888534298874	23.93914294268581	32.64696528132423	17.378003241691086
24-25	17.002972285303876	32.937380038813714	23.42706581818634	26.63258185769607
26-27	17.4017963610012	28.20013772064017	37.1180652081152	17.28000071024343
28-29	26.515679473441704	23.788262078871885	32.455984016462935	17.240074431223473
30-31	23.619854628849023	25.554385786967643	33.039815990604666	17.785943593578672
32-33	19.440835532805757	27.18888613789141	33.79166480317723	19.578613526125604
34-35	20.015006804779137	26.65360350317111	34.10788585284031	19.223503839209442
36-37	19.902211309955167	26.253448894655758	34.98105285867339	18.86328693671568
38-39	21.026161653176594	25.974578123949183	34.060677261186605	18.938582961687626
40-41	19.014839566806685	27.262960968187038	34.722253512623446	18.99994595238283
42-43	19.193591777701027	26.34261162404788	34.55000563791449	19.913790960336605
44-45	19.205695929994594	28.168202355828264	33.505140223010834	19.120961491166312
46-47	20.198139094247917	26.17183390091003	33.37359864935836	20.256428355483695
48-49	19.32382779484863	27.491380252801523	33.34295245140449	19.841839500945355
50-51	20.465827236477182	27.5072338922021	32.9674497891431	19.059489082177617
52-53	20.30556720568508	26.92693358146167	33.31498044868419	19.452518764169056
54-55	19.93664062353475	28.336951760555447	31.03666136526294	20.689746250646863
56-57	20.18628394818541	28.992689470340334	30.536362602271122	20.28466397920314
58-59	22.13638000094999	28.67979315001418	29.066604754317922	20.117222094717903
60-61	21.105159757027298	29.527771725793656	29.11020886910966	20.256859648069387
62-63	20.001114300976468	29.671215730111868	29.04086987395192	21.28680009495975
64-65	21.10104603090924	29.499592131183928	29.17500624289004	20.224355595016785
66-67	21.212788789161173	28.228974377784482	30.263196300551037	20.295040532503304
68-69	21.895867771346573	28.356580552905946	29.778892001835583	19.968659673911898
70-71	19.769518274038273	29.291208155230642	30.670130007336628	20.269143563394458
72-73	19.636073810644657	28.51171305300665	30.584433037718767	21.267780098629927
74-75	19.904076445872406	30.154362369930354	29.331546950694552	20.61001423350269
76-77	20.962258956133446	28.393757037792312	29.206947687487013	21.43703631858723
78-79	20.440815335474888	29.483917311446817	28.763536887550405	21.311730465527884
80-81	21.080157604133085	29.499793867288265	28.86901112339831	20.551037405180338
82-83	20.93377749043249	28.969509809209093	29.485543920438793	20.611168779919627
84-85	20.35316532772878	29.708318948531765	28.501826661726177	21.43668906201328
86-87	20.45037946842239	29.97460962858814	28.92375009571327	20.651260807276206
88-89	22.535438526870557	28.901234214433135	28.004842397903595	20.55848486079271
90-91	21.4439339117562	29.998862537362875	28.08775884904317	20.46944470183775
92-93	20.761458824845406	29.977890002206113	27.83024395570543	21.430407217243047
94-95	21.356136880765547	30.284216862228796	27.828097381861504	20.531548875144153
96-97	21.282473745846993	29.085094938643856	28.69046560845504	20.94196570705411
98-99	22.30049907690969	29.32333196760062	27.520531490496765	20.855637464992927
100	20.780585871081904	29.256511854959545	28.985808316504524	20.977093957454024
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	57184.0
1	32829.0
2	6268.5
3	3383.5
4	2576.5
5	2284.0
6	2141.0
7	2330.0
8	2306.0
9	2164.5
10	2327.0
11	4066.0
12	28221.0
13	31043.0
14	10205.0
15	9917.5
16	11695.0
17	13660.5
18	15606.0
19	17774.5
20	20465.0
21	23606.0
22	27171.0
23	31852.0
24	36715.0
25	41875.5
26	50097.0
27	58799.5
28	64217.0
29	68911.0
30	74792.5
31	80862.5
32	88508.0
33	98264.5
34	108236.5
35	115399.5
36	120232.0
37	124241.0
38	125821.5
39	126131.0
40	127324.5
41	129576.5
42	130692.0
43	131138.0
44	132524.0
45	134109.0
46	140767.0
47	153234.0
48	158761.0
49	169089.0
50	214878.0
51	205483.0
52	139140.5
53	111641.5
54	103604.5
55	98085.0
56	88682.0
57	77871.0
58	73489.0
59	62946.0
60	48311.0
61	38809.5
62	29963.0
63	22471.5
64	16389.5
65	12123.5
66	9312.0
67	7265.0
68	5671.0
69	4483.5
70	3355.0
71	2436.5
72	1928.0
73	1478.5
74	1021.0
75	651.5
76	439.0
77	262.5
78	180.5
79	125.5
80	82.0
81	55.0
82	47.5
83	44.0
84	29.0
85	18.0
86	18.0
87	16.5
88	15.0
89	13.5
90	11.5
91	7.5
92	2.5
93	5.0
94	3.5
95	1.0
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02723233576870758
2	0.012761689541162885
3	0.0052156470298665705
4	0.0
5	0.003351330644722775
6	0.0012428775900958635
7	1.997481841225495E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.0010875178913338807
26-27	0.0038950895903897154
28-29	8.211869791704812E-4
30-31	7.435071297894898E-4
32-33	0.005248938393886995
34-35	0.008511492067888636
36-37	0.00541539521398912
38-39	0.005814891582234219
40-41	0.008578074795929487
42-43	0.01023154587561059
44-45	0.009343776168399261
46-47	0.012317804687557218
48-49	0.016767750344954018
50-51	0.060756739337275475
52-53	0.010597750879835265
54-55	0.00582598870357436
56-57	0.004117032017192548
58-59	0.008411617975827361
60-61	0.016035340336504667
62-63	0.013460808185591807
64-65	0.012162444988795236
66-67	0.010242642996950733
68-69	0.006558398712023708
70-71	0.004361168686675664
72-73	0.003195970945960792
74-75	0.0023525897241100276
76-77	0.0019419962345247868
78-79	0.0014981113809191214
80-81	0.001786636535762804
82-83	0.0013316545608169966
84-85	0.0010986150126740223
86-87	6.214387950479318E-4
88-89	0.001198489104735297
90-91	6.7692440174864E-4
92-93	4.216906109253823E-4
94-95	6.436330377282151E-4
96-97	0.001165197740714872
98-99	0.0034401076154439085
100	1.3316545608169967E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4505673.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.258468625960624
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.45994568331801	50.89426987108556
2	10.813668363215049	13.032301915926741
3	2.5319357988295454	4.577117216901501
4	0.8489304106798266	2.046209860702968
5	0.36700484138137784	1.1057574859977706
6	0.21927460891620792	0.792789128510826
7	0.13739329157656766	0.5795376544918843
8	0.09019743053309918	0.43481272303368157
9	0.0639208168166151	0.34665934812207994
>10	0.3259329671741474	3.8897571954782766
>50	0.11131413560416548	4.79024642532528
>100	0.02559751952717452	2.758977116628376
>500	0.0015423577255516635	0.6552499177005338
>1k	0.003011269512306925	3.8738984491919743
>5k	1.1016839679171506E-4	0.577534234106925
>10k+	2.2033679358343013E-4	9.64488145679558
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	278628	6.183937449521969	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	58104	1.2895742766951797	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG	54599	1.2117834561007867	No Hit
TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	23495	0.5214537317732556	No Hit
AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	10636	0.23605796514749294	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA	10394	0.2306869584188644	No Hit
ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	9737	0.21610534097791828	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9159	0.20327706870871456	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG	7203	0.15986513002608044	No Hit
ACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4933	0.10948419914183741	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.10215765769065E-4	0.0	0.0	0.0	0.0
2	7.10215765769065E-4	0.0	0.0	0.0	0.0
3	7.546042511296315E-4	0.0	0.0	0.0	0.0
4	7.546042511296315E-4	0.0	0.0	0.0	0.0
5	7.546042511296315E-4	0.0	0.0	0.0	0.0
6	7.546042511296315E-4	0.0	0.0	0.0	0.0
7	7.546042511296315E-4	0.0	0.0	0.0	0.0
8	7.767984938099147E-4	0.0	0.0	0.0	0.0
9	7.98992736490198E-4	0.0	0.0	0.0	0.0
10-11	8.211869791704812E-4	2.219424268028328E-5	0.0	0.0	0.0
12-13	8.211869791704812E-4	4.438848536056656E-5	0.0	0.0	0.0
14-15	8.433812218507646E-4	5.54856067007082E-5	0.0	0.0	0.0
16-17	8.988668285514728E-4	7.767984938099147E-5	0.0	0.0	0.0
18-19	9.432553139120393E-4	8.877697072113312E-5	0.0	0.0	0.0
20-21	0.0010320322846331725	1.1097121340141639E-4	0.0	0.0	0.0
22-23	0.0010764207699937391	1.5535969876198294E-4	0.0	0.0	0.0
24-25	0.0010986150126740223	1.997481841225495E-4	0.0	0.0	0.0
26-27	0.0012428775900958635	1.997481841225495E-4	0.0	0.0	0.0
28-29	0.0014426257742184132	2.1084530546269114E-4	0.0	2.219424268028328E-5	0.0
30-31	0.0015757912303001127	2.2194242680283278E-4	0.0	2.219424268028328E-5	0.0
32-33	0.0016756653223613874	2.2194242680283278E-4	0.0	2.219424268028328E-5	0.0
34-35	0.0017422480504022373	2.2194242680283278E-4	0.0	2.219424268028328E-5	0.0
36-37	0.001908704870504362	2.4413666948311608E-4	0.0	2.219424268028328E-5	0.0
38-39	0.0021750357826677614	2.552337908232577E-4	0.0	2.219424268028328E-5	0.0
40-41	0.002652212000293852	2.77428033503541E-4	0.0	2.219424268028328E-5	0.0
42-43	0.00345120473678405	2.885251548436826E-4	0.0	2.219424268028328E-5	0.0
44-45	0.004627499598839064	2.885251548436826E-4	0.0	2.219424268028328E-5	0.0
46-47	0.005970251280996201	2.885251548436826E-4	0.0	2.219424268028328E-5	0.0
48-49	0.007401779933874473	2.996222761838242E-4	0.0	2.219424268028328E-5	0.0
50-51	0.009654495565923227	3.107193975239659E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
52-53	0.012672912570441753	3.99496368245099E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
54-55	0.016534710796811042	3.99496368245099E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
56-57	0.021417444186473365	4.660790962859488E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
58-59	0.02742098683148999	5.326618243267987E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
60-61	0.03518897176958914	5.992445523676486E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
62-63	0.04588659674148568	5.992445523676486E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
64-65	0.06069015660923463	5.992445523676486E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
66-67	0.08019889592520363	5.992445523676486E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
68-69	0.10528948727526387	6.103416737077902E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
70-71	0.13936874691083884	6.214387950479318E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
72-73	0.1857547141126309	6.214387950479318E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
74-75	0.2459565973828993	6.436330377282151E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
76-77	0.3221272382616315	6.436330377282151E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
78-79	0.41762906451489046	6.436330377282151E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
80-81	0.535580367239256	6.436330377282151E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
82-83	0.6830056242430376	6.436330377282151E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
84-85	0.8603265261371609	6.436330377282151E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
86-87	1.07335796450386	6.436330377282151E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
88	1.2530203590007531	6.436330377282151E-4	0.0	2.219424268028328E-5	2.219424268028328E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	6230	0.0	89.88793	1
ATCGAGT	2595	0.0	84.43572	1
TCGAGTG	3145	0.0	76.821915	2
AGTGGTA	129550	0.0	59.253098	5
GTGGTAT	131805	0.0	58.16774	6
GGTATCA	132365	0.0	58.08076	8
TGGTATC	132475	0.0	58.007706	7
GTATCAA	132895	0.0	57.941067	9
ATGAGTG	4760	0.0	57.373566	2
GAGTGGT	135870	0.0	56.455433	4
CGAGTGG	132225	0.0	56.093872	3
ACGAGTG	128350	0.0	55.94072	2
AACGAGT	120040	0.0	54.637997	1
TGAGTGG	5425	0.0	53.62723	3
AATGAGT	4840	0.0	51.876846	1
GACGAGT	1785	0.0	50.048733	1
GAGTACT	46285	0.0	41.324505	20-21
AGTACTT	47240	0.0	41.006397	20-21
ACGATTG	780	0.0	40.97859	2
GTACTTT	46930	0.0	40.08083	22-23
>>END_MODULE