FastQCFastQC Report
Thu 26 May 2016
SRR2132631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132631_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6706682
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3436895.124575758922221No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG541610.807567736177144No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA512280.7638352317882374No Hit
TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT299720.44689758661585566No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA174870.26073996053488147No Hit
AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT133780.19947270498288125No Hit
ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT121970.18186340130633896No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119310.17789720759087727No Hit
AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG77000.11481087071073297No Hit
ACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT74550.11115779755175509No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT76350.087.746581
ATCGAGT33550.077.9124151
TCGAGTG40050.074.176742
AGTGGTA1505300.061.849955
GTGGTAT1520900.061.1669246
TGGTATC1530350.060.9207957
GGTATCA1534400.060.8299878
GTATCAA1539850.060.7245569
GAGTGGT1564200.059.536014
ACGAGTG1461300.059.4064562
CGAGTGG1513850.059.339983
AACGAGT1357600.058.4450651
GACGAGT21250.052.4340971
TGAGTGG76450.049.42623
ATGAGTG72850.048.135162
ACGATTG10400.043.3901372
AGTACTT606200.041.1617420-21
GAGTACT596800.040.99110420-21
AATGAGT78000.040.9260251
GTACTTT603600.039.99627722-23
Produced by FastQC (version 0.11.5)