##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132631_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6706682
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.496847621521344	31.0	16.0	33.0	16.0	34.0
2	27.828548453616854	31.0	25.0	34.0	16.0	34.0
3	28.025352924143412	31.0	25.0	34.0	16.0	34.0
4	33.84805467144558	35.0	33.0	37.0	28.0	37.0
5	34.89565809143776	35.0	35.0	37.0	32.0	37.0
6	35.316381036106975	37.0	35.0	37.0	33.0	37.0
7	35.589913760634545	37.0	35.0	37.0	33.0	37.0
8	35.692785940946656	37.0	35.0	37.0	35.0	37.0
9	37.48948377155798	39.0	37.0	39.0	35.0	39.0
10-11	37.52419214150902	39.0	37.0	39.0	34.5	39.0
12-13	37.67832484080802	39.0	37.0	39.0	35.0	39.0
14-15	39.13860288888007	40.0	39.0	41.0	36.0	41.0
16-17	39.06304003976929	40.5	39.0	41.0	36.0	41.0
18-19	39.07165279343795	40.0	39.0	41.0	35.5	41.0
20-21	39.03061991011353	40.0	39.0	41.0	35.0	41.0
22-23	38.90452767851525	40.0	38.5	41.0	35.0	41.0
24-25	38.7914235384949	40.0	38.0	41.0	34.5	41.0
26-27	38.85836438942535	40.0	38.5	41.0	35.0	41.0
28-29	38.81618608128431	40.0	39.0	41.0	35.0	41.0
30-31	38.71737112330658	40.0	38.0	41.0	35.0	41.0
32-33	38.616478386779036	40.0	38.0	41.0	35.0	41.0
34-35	38.534333445360915	40.0	38.0	41.0	34.0	41.0
36-37	38.25752503249744	40.0	37.5	41.0	34.0	41.0
38-39	37.960766143377604	40.0	37.0	41.0	33.5	41.0
40-41	37.808582843200256	40.0	36.0	41.0	33.0	41.0
42-43	37.698991393956064	40.0	36.0	41.0	33.0	41.0
44-45	37.59362252452107	40.0	36.0	41.0	33.0	41.0
46-47	37.44977426989978	40.0	36.0	41.0	33.0	41.0
48-49	37.29386632615055	40.0	35.0	41.0	33.0	41.0
50-51	37.045828921067084	40.0	35.0	41.0	32.0	41.0
52-53	36.546874445515684	40.0	35.0	41.0	30.5	41.0
54-55	35.858346795032176	39.5	35.0	41.0	28.5	41.0
56-57	35.32744895314852	39.0	35.0	41.0	26.5	41.0
58-59	35.054141824526646	39.0	35.0	41.0	25.0	41.0
60-61	34.7277460001831	39.0	35.0	41.0	24.5	41.0
62-63	34.44768851423103	38.0	35.0	40.5	23.0	41.0
64-65	34.15520811930549	37.5	34.0	40.0	22.5	41.0
66-67	33.809823173366496	37.0	34.0	40.0	21.5	41.0
68-69	33.488799603142056	36.5	34.0	40.0	20.5	41.0
70-71	33.15017917951082	36.0	34.0	39.0	20.0	41.0
72-73	32.874671782559545	35.5	34.0	39.0	20.0	41.0
74-75	32.59977638719116	35.0	33.0	38.5	19.5	40.0
76-77	32.341260551789986	35.0	33.0	37.0	18.5	40.0
78-79	32.01299323570135	35.0	33.0	37.0	17.0	39.0
80-81	31.682587604421975	35.0	33.0	36.5	12.0	39.0
82-83	31.347010339837194	35.0	33.0	36.0	7.5	38.0
84-85	31.053283352334283	35.0	33.0	36.0	4.5	37.0
86-87	30.893645173574654	35.0	33.0	35.5	2.0	37.0
88-89	30.739948382821787	35.0	33.0	35.0	2.0	36.5
90-91	30.54409885543999	35.0	33.0	35.0	2.0	36.0
92-93	30.387667702151376	35.0	33.0	35.0	2.0	36.0
94-95	30.201021160687205	35.0	33.0	35.0	2.0	36.0
96-97	30.026961170963524	35.0	32.5	35.0	2.0	36.0
98-99	29.878539641509768	35.0	32.0	35.0	2.0	35.0
100	29.73633206405194	35.0	32.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.9727882030611497
1101	2	4.140313460915575
1101	3	3.53851750417288
1101	4	1.9764888863819081
1101	5	1.1196395197609732
1101	6	0.8911714654614045
1101	7	0.47373663448802006
1101	8	0.546606513099313
1101	9	0.6490999333481682
1101	10-11	0.4148369808145489
1101	12-13	0.44344150846158215
1101	14-15	0.5312303379634713
1101	16-17	0.5578314892867695
1101	18-19	0.5311151582063616
1101	20-21	0.5428259526668882
1101	22-23	0.4859114171559398
1101	24-25	0.4966150493532737
1101	26-27	0.4371875751950185
1101	28-29	0.4261208483440839
1101	30-31	0.5017958853166604
1101	32-33	0.44183381162066127
1101	34-35	0.46043056900122536
1101	36-37	0.3979183796724257
1101	38-39	0.44596070751093464
1101	40-41	0.3655542479008673
1101	42-43	0.37255837545104953
1101	44-45	0.395103875818144
1101	46-47	0.3622924828023386
1101	48-49	0.3900572051508959
1101	50-51	0.4311082895115206
1101	52-53	0.33834730819038583
1101	54-55	0.2882905011963146
1101	56-57	0.29490548076528
1101	58-59	0.21404296963420677
1101	60-61	0.23575088760149754
1101	62-63	0.2244528215967989
1101	64-65	0.24313148125719408
1101	66-67	0.17910815327884677
1101	68-69	0.15333367451621882
1101	70-71	0.19890541399588813
1101	72-73	0.20082961278009037
1101	74-75	0.2196717630706928
1101	76-77	0.19852465569611155
1101	78-79	0.13847044924537144
1101	80-81	0.08882785473640809
1101	82-83	0.10338854509947737
1101	84-85	0.14484852179926833
1101	86-87	0.1274994873933828
1101	88-89	0.14619747573995312
1101	90-91	0.13100429567298022
1101	92-93	0.13685571044051414
1101	94-95	0.16667953855241535
1101	96-97	0.16121502381059827
1101	98-99	0.22057881216695918
1101	100	0.1898543112197295
1102	1	3.015133172381635
1102	2	3.5585717686597746
1102	3	4.31395698071945
1102	4	2.2089801548823544
1102	5	1.157152497309717
1102	6	0.8995349624737017
1102	7	0.5226915949112296
1102	8	0.5197295532879522
1102	9	0.6533544955419828
1102	10-11	0.445840812017849
1102	12-13	0.3784405382223568
1102	14-15	0.5023090052390344
1102	16-17	0.5266387685817406
1102	18-19	0.4666658040406375
1102	20-21	0.5098984860438591
1102	22-23	0.42336040771068184
1102	24-25	0.4843024782681127
1102	26-27	0.4447423754133055
1102	28-29	0.43331692580672865
1102	30-31	0.45300505085776877
1102	32-33	0.4287850785997378
1102	34-35	0.40912486313192886
1102	36-37	0.43462527445292665
1102	38-39	0.4482132832194381
1102	40-41	0.4626512764419388
1102	42-43	0.4664748099268792
1102	44-45	0.4312019247877359
1102	46-47	0.4622973318201318
1102	48-49	0.501955827829434
1102	50-51	0.5133293156717329
1102	52-53	0.56222699120665
1102	54-55	0.43108861188304815
1102	56-57	0.4230847569258671
1102	58-59	0.3278240350431503
1102	60-61	0.3747867094263597
1102	62-63	0.32729450286497297
1102	64-65	0.3615190395053176
1102	66-67	0.3134472678786864
1102	68-69	0.3197344163418805
1102	70-71	0.3370133699488491
1102	72-73	0.37629303815936765
1102	74-75	0.35817418858163563
1102	76-77	0.3475188744296034
1102	78-79	0.3738121560002945
1102	80-81	0.377274353166122
1102	82-83	0.3681882876353626
1102	84-85	0.36065259046066345
1102	86-87	0.3438490291677354
1102	88-89	0.35397624132902195
1102	90-91	0.37397673138123366
1102	92-93	0.41845289063864044
1102	94-95	0.42088017339470696
1102	96-97	0.4205292899172015
1102	98-99	0.47023816104276506
1102	100	0.5037958952374346
1103	1	2.6986530215496707
1103	2	3.345191723211123
1103	3	3.1187054183415235
1103	4	1.7741607285626202
1103	5	0.9296248899493378
1103	6	0.7786607115603346
1103	7	0.40608527804910466
1103	8	0.4830061364034677
1103	9	0.548919010527328
1103	10-11	0.39402799723127657
1103	12-13	0.3591819370537266
1103	14-15	0.4434285463278229
1103	16-17	0.4973749923625803
1103	18-19	0.4516193252796228
1103	20-21	0.47452925895196785
1103	22-23	0.4115749972275822
1103	24-25	0.4243292766239577
1103	26-27	0.3739051259823114
1103	28-29	0.4078064149361609
1103	30-31	0.43159163548686763
1103	32-33	0.39992014484013083
1103	34-35	0.372237775927303
1103	36-37	0.3806194449789615
1103	38-39	0.3768843782028881
1103	40-41	0.4300359523618127
1103	42-43	0.417080344987653
1103	44-45	0.41147825237384694
1103	46-47	0.4032826103871443
1103	48-49	0.45613271502752184
1103	50-51	0.3947283739517786
1103	52-53	0.34593906758802717
1103	54-55	0.3837843697082164
1103	56-57	0.3657083502571439
1103	58-59	0.3788417047336168
1103	60-61	0.3941033913190779
1103	62-63	0.35981303003580223
1103	64-65	0.40845444007408105
1103	66-67	0.4257983111271102
1103	68-69	0.3854876492013659
1103	70-71	0.413592385647533
1103	72-73	0.4467940511232982
1103	74-75	0.4453187519551207
1103	76-77	0.42507288563524526
1103	78-79	0.396799797505178
1103	80-81	0.4314533289496083
1103	82-83	0.46712077552994913
1103	84-85	0.45102756374095065
1103	86-87	0.4006461370184198
1103	88-89	0.42917967857334816
1103	90-91	0.42875048987836095
1103	92-93	0.44919836350082676
1103	94-95	0.4884171226624545
1103	96-97	0.4531709920575899
1103	98-99	0.4746781455408211
1103	100	0.4933800939800044
1104	1	2.545160990643147
1104	2	3.344933085423154
1104	3	1.602508529257424
1104	4	1.1167364896828005
1104	5	0.6370710924530485
1104	6	0.6198622310328901
1104	7	0.37680614222563236
1104	8	0.4254323096623196
1104	9	0.46448473590942996
1104	10-11	0.40602841743404383
1104	12-13	0.3617831383529051
1104	14-15	0.48461277953150983
1104	16-17	0.5241176764067248
1104	18-19	0.3879681796338872
1104	20-21	0.43087446870090673
1104	22-23	0.3976382421188802
1104	24-25	0.44247587000013766
1104	26-27	0.4248332997980313
1104	28-29	0.4157209499963912
1104	30-31	0.3819288438319006
1104	32-33	0.37794174899610056
1104	34-35	0.4114762937799057
1104	36-37	0.40594618364061574
1104	38-39	0.4200814567745539
1104	40-41	0.3896203623553589
1104	42-43	0.37148599007520033
1104	44-45	0.32478154048880725
1104	46-47	0.322775679808025
1104	48-49	0.401875885748197
1104	50-51	0.43494438823228165
1104	52-53	0.40417408060265103
1104	54-55	0.36706249738475094
1104	56-57	0.35286323962695576
1104	58-59	0.36893869544821456
1104	60-61	0.3620399618041432
1104	62-63	0.35687567681756605
1104	64-65	0.32288891465373126
1104	66-67	0.3006567391070689
1104	68-69	0.2792766518762946
1104	70-71	0.26035487491246556
1104	72-73	0.2621171742602826
1104	74-75	0.3120752804554243
1104	76-77	0.31276079017015945
1104	78-79	0.3001587071562106
1104	80-81	0.34110475443885946
1104	82-83	0.2688717713690707
1104	84-85	0.3042243306605137
1104	86-87	0.30577626515272627
1104	88-89	0.3479816446631787
1104	90-91	0.31505941750664235
1104	92-93	0.33228701905296276
1104	94-95	0.34142350128794874
1104	96-97	0.3877699108589887
1104	98-99	0.43121620128619753
1104	100	0.3925055920089413
1105	1	1.3979042220879663
1105	2	2.7341736703256387
1105	3	1.5875954047482033
1105	4	1.0447177880765466
1105	5	0.6445581580470332
1105	6	0.5636622591117018
1105	7	0.25403016316745664
1105	8	0.33763080198748696
1105	9	0.464747447982532
1105	10-11	0.318232444281044
1105	12-13	0.29403821735566993
1105	14-15	0.39122498447231635
1105	16-17	0.407136900238271
1105	18-19	0.32793487856834247
1105	20-21	0.34627140689172364
1105	22-23	0.30679574318580194
1105	24-25	0.3830797244137756
1105	26-27	0.3344817484944045
1105	28-29	0.2624172974404715
1105	30-31	0.25440268369584373
1105	32-33	0.21707683524959265
1105	34-35	0.22834705228612506
1105	36-37	0.23787942258242367
1105	38-39	0.17607411261640493
1105	40-41	0.14097124266167071
1105	42-43	0.20764005085288773
1105	44-45	0.2646487068872503
1105	46-47	0.2488775637541849
1105	48-49	0.24295216745332482
1105	50-51	0.240508943732749
1105	52-53	0.1625661928427533
1105	54-55	0.2314631708700503
1105	56-57	0.3484556911484731
1105	58-59	0.2953657325521064
1105	60-61	0.1894161313209608
1105	62-63	-0.11451047717973495
1105	64-65	0.02042616668239816
1105	66-67	0.1632596704234004
1105	68-69	0.21971940854545835
1105	70-71	0.2744274248773877
1105	72-73	0.2570952684705432
1105	74-75	0.22315667524632232
1105	76-77	0.2224539466323634
1105	78-79	0.21917131291825953
1105	80-81	0.22495208744672013
1105	82-83	0.22365634010668956
1105	84-85	0.23717022207776495
1105	86-87	0.26761196703291645
1105	88-89	0.20563638341978674
1105	90-91	0.23759032895353016
1105	92-93	0.23410004462626333
1105	94-95	0.21681763921381503
1105	96-97	0.20821208614948006
1105	98-99	0.20574851515045367
1105	100	0.2549277120202227
1106	1	1.912456159563856
1106	2	2.8964165265370276
1106	3	1.3072340484471603
1106	4	0.9133031338690571
1106	5	0.5525175937162601
1106	6	0.5168136119352553
1106	7	0.29537422848441963
1106	8	0.35819228692834315
1106	9	0.3937037165585551
1106	10-11	0.25596804905117665
1106	12-13	0.2067966431276389
1106	14-15	0.18742190866311859
1106	16-17	0.2916077263721206
1106	18-19	0.09978327953605515
1106	20-21	0.22169826490162592
1106	22-23	0.22642138027210734
1106	24-25	0.2581628410834327
1106	26-27	0.20694857484016893
1106	28-29	0.23840099468727516
1106	30-31	0.23644303917370024
1106	32-33	0.14549803283599516
1106	34-35	0.1933324954074962
1106	36-37	0.2371367891474776
1106	38-39	0.2494804459877713
1106	40-41	0.18894925775070703
1106	42-43	0.20271270959469234
1106	44-45	0.1414625010961501
1106	46-47	0.11805111917118438
1106	48-49	0.02645456128133361
1106	50-51	0.0960034310344895
1106	52-53	0.02361334062504028
1106	54-55	0.06758376526206433
1106	56-57	0.1371024889408119
1106	58-59	0.2093184149706957
1106	60-61	0.07215264621437711
1106	62-63	0.20212590581373036
1106	64-65	-0.05008257016156392
1106	66-67	-0.08531135960522818
1106	68-69	0.17370120641930242
1106	70-71	0.2746102221209341
1106	72-73	0.29680723312787194
1106	74-75	0.27405308725505506
1106	76-77	0.21793677213048568
1106	78-79	0.19094810110675908
1106	80-81	0.24461448604268554
1106	82-83	0.22384555575700915
1106	84-85	0.19756414415219226
1106	86-87	0.24844269055376245
1106	88-89	0.21557309589146456
1106	90-91	0.21343135248904588
1106	92-93	0.2460222861376664
1106	94-95	0.1691194700558576
1106	96-97	0.1970161009478133
1106	98-99	0.27456676590707474
1106	100	0.33155791941965873
1107	1	1.5795178107801284
1107	2	0.9767027081339208
1107	3	0.44810264129082356
1107	4	0.3722036561665405
1107	5	0.26922180596556444
1107	6	0.39336233251672326
1107	7	0.1761120033475123
1107	8	0.23031975141677918
1107	9	0.2645943995027338
1107	10-11	0.19091890867590422
1107	12-13	0.17295813298512996
1107	14-15	0.21125846350414434
1107	16-17	0.25805229162300947
1107	18-19	0.171384770701124
1107	20-21	0.2176524010140639
1107	22-23	0.13007560433407406
1107	24-25	0.08612855749451853
1107	26-27	0.13956301489346146
1107	28-29	0.18274417683607425
1107	30-31	0.17429215739502268
1107	32-33	0.1522789937484319
1107	34-35	0.14042975240528932
1107	36-37	0.12074282246280887
1107	38-39	0.17360822432387124
1107	40-41	0.18189376688795988
1107	42-43	0.16508201810403023
1107	44-45	0.15267179737222847
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2216	82-83	-1.1194253809822179
2216	84-85	-1.1144459432995006
2216	86-87	-1.1177562178223575
2216	88-89	-1.1184382579542067
2216	90-91	-1.1274947180800226
2216	92-93	-1.1513521256908348
2216	94-95	-1.1659851057154142
2216	96-97	-1.139107976502995
2216	98-99	-1.2013794453323783
2216	100	-1.2740427213737213
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4913.0
3	135.0
4	286.0
5	512.0
6	1034.0
7	1829.0
8	2653.0
9	3876.0
10	6204.0
11	10083.0
12	11305.0
13	14133.0
14	15212.0
15	17713.0
16	19594.0
17	26118.0
18	38345.0
19	65326.0
20	114354.0
21	121109.0
22	59721.0
23	40862.0
24	39116.0
25	41517.0
26	45737.0
27	54883.0
28	69210.0
29	86164.0
30	106196.0
31	132212.0
32	167124.0
33	220149.0
34	307188.0
35	491925.0
36	747823.0
37	1297058.0
38	1713008.0
39	609118.0
40	2937.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.431183082597602	38.970717528602904	27.137415788603164	16.46068360019633
2	19.22770763562746	37.38340501641389	25.433237507355454	17.955649840603193
3	20.569272904066903	24.572592763023597	22.05822904969037	32.799905283219125
4	31.581697179022356	25.89858889984645	23.495522823357362	19.024191097773834
5	17.68894834752766	37.189529580242024	24.531748308639642	20.589773763590678
6	33.93661838895156	23.18527650465645	25.04736369202104	17.830741414370948
7	19.260004786283496	23.1399212574357	38.46412486683023	19.13594908945057
8	33.157647850308095	23.211283910583504	25.015096883973325	18.615971355135073
9	32.79247961845807	24.82640817417736	24.86347568939092	17.517636517973646
10-11	17.5722063458503	32.34702346107956	31.990871193833254	18.08989899923688
12-13	17.434299106473215	24.958220771463445	31.887705425723183	25.71977469634016
14-15	17.68577964483779	39.68150718939708	24.230222932889912	18.402490232875213
16-17	25.362944001221464	24.56982454215065	24.33325450647578	25.733976950152105
18-19	18.089518781418292	32.16179028616535	24.404660903856783	25.344030028559576
20-21	25.526884978294785	32.05649231617065	24.5492555036902	17.86736720184437
22-23	25.05974638427765	24.756690417109382	32.11551554106784	18.068047657545115
24-25	17.786847101510105	31.99585245190083	24.578904433579215	25.638396013009846
26-27	18.10582079851176	27.93030919036015	35.93293233634302	18.03093767478507
28-29	25.184589314031086	24.464109849261444	32.333789845742544	18.01751099096493
30-31	23.099427172389163	25.7705998195073	32.72806027755319	18.401912730550347
32-33	19.898565254387222	27.075007151771363	33.26172739527678	19.764700198564636
34-35	20.099767944764828	26.653881907415894	33.57778264229227	19.668567505527008
36-37	20.05690413728507	26.39015478799138	34.29537440281559	19.257566671907956
38-39	20.778781768790335	26.2058131208858	33.557692967228355	19.45771214309551
40-41	19.540573882268347	27.0691718898581	33.98907480284139	19.40117942503216
42-43	19.61189378208402	26.46930185914905	33.798196398015214	20.120607960751713
44-45	19.61697031006938	27.734736610423443	33.10867012063026	19.53962295887692
46-47	20.372478383749815	26.361837741956045	32.950647508888636	20.3150363654055
48-49	19.819630789599405	27.335228306881326	32.910554549312224	19.93458635420705
50-51	20.557803922691978	27.344970833889082	32.71592754021474	19.3812977032042
52-53	20.40158586033162	26.96627638508902	32.839311525343	19.792826229236358
54-55	20.308417339724304	28.075767166418018	31.02644738146842	20.589368112389256
56-57	20.60821782505965	28.4963078805078	30.4448734844059	20.45060081002665
58-59	21.925201031528914	28.559286220643042	29.26226655425264	20.2532461935754
60-61	21.163004453024918	29.10334863805112	29.325541316437377	20.408105592486596
62-63	20.369594095632806	29.199734976325033	29.273014648776524	21.15765627926564
64-65	21.111773546212603	29.17589858956996	29.339560488393946	20.372767375823493
66-67	21.187248262549787	28.252288857794106	29.981011835709694	20.579451043946413
68-69	21.72402897423934	28.39836462586373	29.66276298482322	20.21484341507371
70-71	20.146287684948106	29.026167566395227	30.25221749844122	20.575327250215448
72-73	20.037706963896788	28.54164276245261	30.248610536105257	21.172039737545337
74-75	20.26336340981993	29.5054023980422	29.425845400273243	20.805388791864623
76-77	21.039605012273473	28.380755345064422	29.32791324882624	21.251726393835867
78-79	20.70486670315823	29.17592934573291	28.91127107689725	21.207932874211615
80-81	21.008474406945098	29.107822417083252	29.102491804276003	20.781211371695655
82-83	21.062189749633177	28.83327484599266	29.38488986822567	20.719645536148494
84-85	20.66642764819015	29.361570913523995	28.768236994200258	21.203764444085593
86-87	20.824186609785695	29.578873105766206	28.88183965240997	20.71510063203813
88-89	22.11810488773765	28.86621434038866	28.27302163044272	20.742659141430977
90-91	21.524531353780212	29.62604252224086	28.235090894736054	20.61433522924288
92-93	20.910899627988943	29.72189342362971	28.10409748942673	21.263109458954617
94-95	21.265737492368565	29.967552284599336	27.98863155750749	20.778078665524614
96-97	21.22103961375938	29.02675000650479	28.681850771848282	21.07035960788755
98-99	21.961010435424303	29.39103790679863	27.697217098412413	20.95073455936466
100	21.040233856916963	29.238719989741547	28.68363038857614	21.03741576476535
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	54806.0
1	31786.5
2	6495.0
3	3618.0
4	2823.5
5	2452.5
6	2306.5
7	2401.0
8	2299.5
9	2178.0
10	2389.0
11	4004.0
12	26284.5
13	29810.0
14	11927.5
15	12428.5
16	14815.0
17	17797.0
18	20992.5
19	24429.0
20	28350.5
21	32998.0
22	38893.0
23	46652.0
24	54694.5
25	63041.0
26	76227.5
27	89894.0
28	98671.0
29	107163.0
30	116842.5
31	126551.5
32	137664.0
33	151206.5
34	167615.5
35	180597.5
36	190073.0
37	198309.5
38	202977.0
39	204969.5
40	206311.5
41	208412.0
42	210362.0
43	211607.5
44	212397.0
45	214507.5
46	221848.5
47	233655.5
48	237275.5
49	244646.5
50	287438.5
51	271983.5
52	199036.5
53	167316.5
54	153288.5
55	140655.5
56	125523.0
57	111057.0
58	107057.5
59	92449.0
60	68685.0
61	54022.0
62	41056.5
63	30978.5
64	23346.5
65	17059.0
66	12862.0
67	10347.5
68	8206.0
69	6050.0
70	4280.5
71	3159.0
72	2458.0
73	1652.0
74	1153.0
75	790.0
76	547.0
77	402.0
78	246.5
79	135.0
80	87.0
81	67.0
82	49.5
83	33.0
84	27.5
85	24.0
86	19.0
87	16.5
88	10.0
89	11.5
90	10.0
91	6.5
92	7.0
93	4.5
94	3.0
95	4.0
96	3.0
97	1.0
98	0.5
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025064555021395082
2	0.01157055008721153
3	0.005561617503260181
4	0.0
5	0.003667983661667573
6	0.0010139141828999795
7	2.534785457249949E-4
8	0.0
9	1.491050268970558E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.001028824685589685
26-27	0.0034443261213219895
28-29	5.516885995191065E-4
30-31	5.889648562433703E-4
32-33	0.004495516560946233
34-35	0.007753461398646902
36-37	0.004704263598602111
38-39	0.0050099289037410745
40-41	0.007947297933613075
42-43	0.009796200267136566
44-45	0.00849898653313218
46-47	0.011771841873522556
48-49	0.015618751567466596
50-51	0.06023843086641054
52-53	0.009975126299413033
54-55	0.005173944433327836
56-57	0.0035188786347705166
58-59	0.007753461398646902
60-61	0.015141615481396016
62-63	0.012912495329285033
64-65	0.011689834108729175
66-67	0.009878208031929948
68-69	0.006694815707677805
70-71	0.0045849795770844665
72-73	0.003407049864597725
74-75	0.002527330205905096
76-77	0.0021545676386624563
78-79	0.0016252447931779084
80-81	0.002027828365799959
82-83	0.001528326525694822
84-85	0.0013046689853492383
86-87	6.784278723816039E-4
88-89	0.0013792214987977662
90-91	7.529803858301318E-4
92-93	3.9512832127719784E-4
94-95	6.933383750713096E-4
96-97	0.001274847979969827
98-99	0.0036754389130124257
100	2.534785457249949E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6706682.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.42174980791069
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.88089015395215	54.68175468970655
2	10.620821292417476	13.684237841092987
3	2.5188217162615	4.868007072471916
4	0.7774892855276635	2.003488809223775
5	0.32428755523430275	1.0445585874561636
6	0.1817494424059759	0.7025170263843031
7	0.13122498809893393	0.5917620346298905
8	0.0896459980247424	0.4620121644824328
9	0.06498974280149158	0.3768077655754341
>10	0.3049336996989346	3.651073810822218
>50	0.07199128111233127	3.3547336917884016
>100	0.029668272292584705	3.0895560383827356
>500	0.0012699672427316042	0.5535618325571653
>1k	0.001962618947854376	2.691808533597816
>5k	6.926890402369526E-5	0.3003657597417288
>10k+	1.847170773965207E-4	7.943754342086469
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	343689	5.124575758922221	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG	54161	0.807567736177144	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	51228	0.7638352317882374	No Hit
TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	29972	0.44689758661585566	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA	17487	0.26073996053488147	No Hit
AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13378	0.19947270498288125	No Hit
ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12197	0.18186340130633896	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11931	0.17789720759087727	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG	7700	0.11481087071073297	No Hit
ACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7455	0.11115779755175509	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.7276256724263953E-4	0.0	0.0	0.0	0.0
2	3.8767306993234507E-4	0.0	0.0	0.0	0.0
3	4.1749407531175626E-4	0.0	0.0	0.0	0.0
4	4.1749407531175626E-4	0.0	0.0	0.0	0.0
5	4.324045780014618E-4	1.491050268970558E-5	0.0	0.0	0.0
6	4.324045780014618E-4	4.473150806911674E-5	0.0	0.0	0.0
7	4.324045780014618E-4	4.473150806911674E-5	0.0	1.491050268970558E-5	0.0
8	4.324045780014618E-4	4.473150806911674E-5	0.0	1.491050268970558E-5	0.0
9	4.62225583380873E-4	4.473150806911674E-5	0.0	1.491050268970558E-5	0.0
10-11	5.069570914499897E-4	5.964201075882232E-5	0.0	1.491050268970558E-5	0.0
12-13	5.218675941396953E-4	5.964201075882232E-5	0.0	1.491050268970558E-5	0.0
14-15	5.36778096829401E-4	5.964201075882232E-5	0.0	2.236575403455837E-5	0.0
16-17	5.591438508639593E-4	5.964201075882232E-5	0.0	2.982100537941116E-5	0.0
18-19	5.740543535536648E-4	7.45525134485279E-5	0.0	2.982100537941116E-5	0.0
20-21	5.964201075882232E-4	8.946301613823348E-5	0.0	2.982100537941116E-5	0.0
22-23	6.336963643124872E-4	8.946301613823348E-5	0.0	2.982100537941116E-5	0.0
24-25	7.08248877761015E-4	9.691826748308628E-5	0.0	2.982100537941116E-5	0.0
26-27	7.902566425543958E-4	1.0437351882793906E-4	0.0	2.982100537941116E-5	0.0
28-29	8.051671452441013E-4	1.0437351882793906E-4	0.0	2.982100537941116E-5	0.0
30-31	9.020854127271877E-4	1.0437351882793906E-4	0.0	2.982100537941116E-5	0.0
32-33	9.915484288654211E-4	1.0437351882793906E-4	0.0	2.982100537941116E-5	0.0
34-35	0.0010884666963485072	1.0437351882793906E-4	0.0	2.982100537941116E-5	0.0
36-37	0.0014612292635911468	1.0437351882793906E-4	0.0	2.982100537941116E-5	0.0
38-39	0.0017519840660404056	1.0437351882793906E-4	0.0	2.982100537941116E-5	0.0
40-41	0.0022589411574903953	1.1182877017279185E-4	0.0	2.982100537941116E-5	0.0
42-43	0.0029299137785271465	1.491050268970558E-4	0.0	2.982100537941116E-5	0.0
44-45	0.0038170886885646287	1.6401552958676137E-4	0.0	2.982100537941116E-5	0.0
46-47	0.004637166336498435	1.7892603227646697E-4	0.0	2.982100537941116E-5	0.0
48-49	0.005785275043605764	1.7892603227646697E-4	0.0	5.218675941396953E-5	0.0
50-51	0.007201772799127796	1.7892603227646697E-4	0.0	1.1182877017279185E-4	0.0
52-53	0.009326519432410842	1.7892603227646697E-4	0.0	4.398598293463146E-4	0.0
54-55	0.012152059692110048	1.7892603227646697E-4	0.0	6.784278723816039E-4	0.0
56-57	0.015760401343018798	1.7892603227646697E-4	0.0	7.306146317955734E-4	0.0
58-59	0.020308104663378998	1.7892603227646697E-4	0.0	7.306146317955734E-4	0.0
60-61	0.026876181098194306	1.7892603227646697E-4	0.0	7.306146317955734E-4	0.0
62-63	0.0358001169579831	1.7892603227646697E-4	0.0	7.306146317955734E-4	0.0
64-65	0.04764651134495418	2.0874703765587813E-4	0.0	7.306146317955734E-4	0.0
66-67	0.06404060905228547	2.0874703765587813E-4	0.0	7.306146317955734E-4	0.0
68-69	0.08554900918218578	2.0874703765587813E-4	0.0	7.306146317955734E-4	0.0
70-71	0.11405043507355798	2.0874703765587813E-4	0.0	7.306146317955734E-4	0.0
72-73	0.15051406940123296	2.0874703765587813E-4	0.0	7.306146317955734E-4	0.0
74-75	0.19784746018970334	2.0874703765587813E-4	0.0	7.306146317955734E-4	0.0
76-77	0.26123200712364175	2.0874703765587813E-4	0.0	7.306146317955734E-4	0.0
78-79	0.3392288466934916	2.0874703765587813E-4	0.0	7.455251344852791E-4	0.0
80-81	0.4373697753971338	2.0874703765587813E-4	0.0	7.455251344852791E-4	0.0
82-83	0.55759315858423	2.236575403455837E-4	1.491050268970558E-5	7.455251344852791E-4	0.0
84-85	0.701166388983405	2.311127916904365E-4	1.491050268970558E-5	7.455251344852791E-4	0.0
86-87	0.8754179786666492	2.4602329438014206E-4	1.491050268970558E-5	7.455251344852791E-4	0.0
88	1.0268415887319542	2.534785457249949E-4	1.491050268970558E-5	7.455251344852791E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	7635	0.0	87.74658	1
ATCGAGT	3355	0.0	77.912415	1
TCGAGTG	4005	0.0	74.17674	2
AGTGGTA	150530	0.0	61.84995	5
GTGGTAT	152090	0.0	61.166924	6
TGGTATC	153035	0.0	60.920795	7
GGTATCA	153440	0.0	60.829987	8
GTATCAA	153985	0.0	60.724556	9
GAGTGGT	156420	0.0	59.53601	4
ACGAGTG	146130	0.0	59.406456	2
CGAGTGG	151385	0.0	59.33998	3
AACGAGT	135760	0.0	58.445065	1
GACGAGT	2125	0.0	52.434097	1
TGAGTGG	7645	0.0	49.4262	3
ATGAGTG	7285	0.0	48.13516	2
ACGATTG	1040	0.0	43.390137	2
AGTACTT	60620	0.0	41.16174	20-21
GAGTACT	59680	0.0	40.991104	20-21
AATGAGT	7800	0.0	40.926025	1
GTACTTT	60360	0.0	39.996277	22-23
>>END_MODULE