FastQCFastQC Report
Thu 26 May 2016
SRR2132630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132630_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6587974
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3631175.511815923985128No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG1427552.1669029052027224No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA733921.1140298975071852No Hit
TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT314460.4773242881650717No Hit
AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG209310.3177152793863485No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA153950.23368337519243396No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150050.22776349754871525No Hit
AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT140330.21300934095975485No Hit
ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT131500.19960613080743794No Hit
GTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTT100330.15229264717802468No Hit
TACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG100160.15203460122945234No Hit
ACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACT96580.1466004571359875No Hit
ACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTC93330.14166722576622193No Hit
CCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGAT88690.13462408928754122No Hit
ACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAGA80330.12193430028715961No Hit
ACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT77390.11747162329420244No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGTAACGAGTGGTATCAACGCA76030.11540725570562362No Hit
ATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTA74020.11235624184309168No Hit
TGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTC72230.10963916979635925No Hit
CACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCT71610.10869806104274243No Hit
GCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCC71480.1085007317879518No Hit
CCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATA69980.10622385577113691No Hit
TCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCG69280.10516131362995665No Hit
AACGAGTGGTATCAACGCAGAGTACATGGAAACGAGTGGTATCAACGCAG68700.10428092157012156No Hit
TTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTG66960.10163974539061629No Hit
GAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAGAGT66940.10160938704372542No Hit
TCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGT66140.10039505316809083No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT92800.087.844911
ATCGAGT38950.082.5589451
TCGAGTG49350.075.3360062
TGAGTGG79900.056.703163
ATGAGTG76050.056.550342
GACGAGT26900.055.9259261
AATGAGT75350.052.7216451
AGTGGTA2412800.046.945
GTGGTAT2443900.046.310846
TGGTATC2453850.046.2203757
GTATCAA2486750.045.782739
GGTATCA2478950.045.7789238
GAGTGGT2587050.043.7838174
CGAGTGG2536200.043.0784873
ACGAGTG2470500.042.7153052
TTCGAGT11050.041.694551
AACGAGT2350300.040.995871
AGTACTT652900.039.5740420-21
GAGTACT644350.039.3479320-21
ACGATTG12100.039.23282
Produced by FastQC (version 0.11.5)