##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132630_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6587974
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.552322307282935	31.0	22.0	33.0	16.0	34.0
2	27.866463498489825	31.0	25.0	34.0	16.0	34.0
3	28.04750352688095	31.0	25.0	34.0	16.0	34.0
4	33.87381143277129	35.0	33.0	37.0	28.0	37.0
5	34.899686914368516	35.0	35.0	37.0	32.0	37.0
6	35.31597453177562	37.0	35.0	37.0	33.0	37.0
7	35.56570639167671	37.0	35.0	37.0	33.0	37.0
8	35.68754491137943	37.0	35.0	37.0	35.0	37.0
9	37.48894455260449	39.0	37.0	39.0	35.0	39.0
10-11	37.48590689641459	39.0	37.0	39.0	34.5	39.0
12-13	37.68569942747193	39.0	37.0	39.0	35.0	39.0
14-15	39.15188607301729	40.5	39.0	41.0	36.0	41.0
16-17	39.055073760157526	40.5	39.0	41.0	36.0	41.0
18-19	39.04489566898716	40.0	38.5	41.0	35.5	41.0
20-21	38.98764749223358	40.0	39.0	41.0	35.0	41.0
22-23	38.82344002875543	40.0	38.5	41.0	35.0	41.0
24-25	38.65842890393921	40.0	38.0	41.0	34.5	41.0
26-27	38.76213946806712	40.0	38.0	41.0	35.0	41.0
28-29	38.71504722089067	40.0	38.0	41.0	35.0	41.0
30-31	38.560115446721554	40.0	38.0	41.0	34.5	41.0
32-33	38.444418875970065	40.0	38.0	41.0	34.0	41.0
34-35	38.33420358064558	40.0	38.0	41.0	34.0	41.0
36-37	37.97271384191862	40.0	37.5	41.0	33.5	41.0
38-39	37.65963276418516	40.0	36.0	41.0	33.0	41.0
40-41	37.44143100746906	40.0	35.0	41.0	33.0	41.0
42-43	37.39349768229201	40.0	35.0	41.0	33.0	41.0
44-45	37.28191375679381	40.0	35.0	41.0	33.0	41.0
46-47	37.10754899761292	40.0	35.0	41.0	32.5	41.0
48-49	36.928704029493744	40.0	35.0	41.0	32.0	41.0
50-51	36.644416477660656	40.0	35.0	41.0	31.0	41.0
52-53	36.04003081979376	39.5	35.0	41.0	29.5	41.0
54-55	35.2764491784576	39.0	35.0	41.0	27.0	41.0
56-57	34.76015236247137	39.0	35.0	41.0	23.5	41.0
58-59	34.4780370262542	39.0	35.0	41.0	22.0	41.0
60-61	34.07250370144145	38.0	34.0	40.0	19.5	41.0
62-63	33.770059657187474	37.5	34.0	40.0	18.0	41.0
64-65	33.40286687530947	37.0	34.0	40.0	15.5	41.0
66-67	32.94962191714782	36.0	33.5	40.0	11.5	41.0
68-69	32.64364044241826	36.0	33.0	39.0	9.0	41.0
70-71	32.25505747290441	35.0	33.0	39.0	7.0	41.0
72-73	32.05248480033467	35.0	33.0	38.5	4.5	40.5
74-75	31.800557652474037	35.0	33.0	37.0	2.0	40.0
76-77	31.527604844827863	35.0	33.0	37.0	2.0	39.5
78-79	31.19390900753403	35.0	33.0	36.5	2.0	39.0
80-81	30.845241723783367	35.0	32.5	36.0	2.0	39.0
82-83	30.469909034249376	35.0	32.0	36.0	2.0	37.5
84-85	30.193629407159165	35.0	32.0	35.0	2.0	37.0
86-87	30.073668171732308	35.0	32.0	35.0	2.0	37.0
88-89	29.920627646678632	35.0	32.0	35.0	2.0	36.0
90-91	29.682931049818958	35.0	32.0	35.0	2.0	36.0
92-93	29.533925908025743	35.0	32.0	35.0	2.0	36.0
94-95	29.287293331758747	35.0	31.0	35.0	2.0	36.0
96-97	29.046608183335273	35.0	31.0	35.0	2.0	35.0
98-99	28.90266484051091	35.0	31.0	35.0	2.0	35.0
100	28.70115137066418	35.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.93818060611995
1101	2	4.094507972509842
1101	3	3.409357375314478
1101	4	1.9098722634792153
1101	5	1.0866040186543344
1101	6	0.8868722835276799
1101	7	0.5020979345475709
1101	8	0.5259381635533131
1101	9	0.6325065425122745
1101	10-11	0.4684646748535002
1101	12-13	0.4296305197410959
1101	14-15	0.5244813547097777
1101	16-17	0.5416930390215668
1101	18-19	0.50691162130245
1101	20-21	0.4969852151508576
1101	22-23	0.5109435679301697
1101	24-25	0.5290196986346842
1101	26-27	0.4861299596675508
1101	28-29	0.4762823670713914
1101	30-31	0.5332534536902216
1101	32-33	0.5054859964571676
1101	34-35	0.5274784808513004
1101	36-37	0.45341611136339566
1101	38-39	0.5186744016501805
1101	40-41	0.4961754011088573
1101	42-43	0.5368137329454115
1101	44-45	0.5147478315795126
1101	46-47	0.5421148228563766
1101	48-49	0.5257479798725839
1101	50-51	0.5851972705922108
1101	52-53	0.5016076878788027
1101	54-55	0.4567701838792999
1101	56-57	0.4641571067996537
1101	58-59	0.4450669686024611
1101	60-61	0.44085078366912
1101	62-63	0.43658833911447203
1101	64-65	0.47498031964560994
1101	66-67	0.41187611509052857
1101	68-69	0.45129398137883214
1101	70-71	0.45275733713007327
1101	72-73	0.4313675680577447
1101	74-75	0.39075965606675567
1101	76-77	0.44329338544073593
1101	78-79	0.502608434089602
1101	80-81	0.4816231048501507
1101	82-83	0.4827663499961865
1101	84-85	0.4582181178752016
1101	86-87	0.43158399931738245
1101	88-89	0.4842018622219264
1101	90-91	0.4799412166646633
1101	92-93	0.4969524526984834
1101	94-95	0.5130128997289205
1101	96-97	0.5226301186639084
1101	98-99	0.6141778421104611
1101	100	0.5724829535165554
1102	1	2.9902964298003205
1102	2	3.557148034655466
1102	3	4.21627691795932
1102	4	2.109578429801374
1102	5	1.1070537412565074
1102	6	0.8644013560979857
1102	7	0.44756488492564017
1102	8	0.49166544853521543
1102	9	0.5479167369932654
1102	10-11	0.41133205385077787
1102	12-13	0.34630518595847803
1102	14-15	0.4810257014057697
1102	16-17	0.5410425705362698
1102	18-19	0.44116003515628677
1102	20-21	0.45977913457258524
1102	22-23	0.36019972917706866
1102	24-25	0.41906768206580125
1102	26-27	0.3680368122182074
1102	28-29	0.38327457428223966
1102	30-31	0.37733419143749813
1102	32-33	0.3761618169991223
1102	34-35	0.3651545072556317
1102	36-37	0.3777717143155144
1102	38-39	0.39188405980994645
1102	40-41	0.3270944629713881
1102	42-43	0.3915010078677241
1102	44-45	0.3948228769351374
1102	46-47	0.40807734087318437
1102	48-49	0.3882450482026343
1102	50-51	0.43001718802065625
1102	52-53	0.3632940044735733
1102	54-55	0.27609398529354223
1102	56-57	0.2811903519947023
1102	58-59	0.2171517306472026
1102	60-61	0.23199400161341543
1102	62-63	0.1612121489219831
1102	64-65	0.1368723175026645
1102	66-67	0.13118833241029648
1102	68-69	0.14553897549699002
1102	70-71	0.19333402695696833
1102	72-73	0.1723747306968093
1102	74-75	0.21506405932519357
1102	76-77	0.20222659708584345
1102	78-79	0.23543769232141898
1102	80-81	0.22650844389532665
1102	82-83	0.2342953560523604
1102	84-85	0.23570943616288176
1102	86-87	0.26675879004643477
1102	88-89	0.2637561975211149
1102	90-91	0.26128735819393256
1102	92-93	0.27694665410002983
1102	94-95	0.27843937976654587
1102	96-97	0.22066828713764508
1102	98-99	0.2798019434857011
1102	100	0.30180921780331715
1103	1	2.6162097434812814
1103	2	3.2913721389781614
1103	3	3.1171736261081584
1103	4	1.7179935286782424
1103	5	0.9100396510967599
1103	6	0.7455787712638511
1103	7	0.4178530512994598
1103	8	0.4559027770333657
1103	9	0.5416681747617886
1103	10-11	0.3794630308397373
1103	12-13	0.3786685339801963
1103	14-15	0.44003050331257043
1103	16-17	0.5047151706720854
1103	18-19	0.44486943557636494
1103	20-21	0.4360644690342994
1103	22-23	0.4079008877026524
1103	24-25	0.4264077810245652
1103	26-27	0.37826647052137474
1103	28-29	0.41057395989167844
1103	30-31	0.40697171486024786
1103	32-33	0.40154595090318423
1103	34-35	0.37365880097045334
1103	36-37	0.37517221282603685
1103	38-39	0.3864814015917659
1103	40-41	0.39454077972145285
1103	42-43	0.3922890104550234
1103	44-45	0.39188298510164543
1103	46-47	0.41324429354650505
1103	48-49	0.3525114192012708
1103	50-51	0.3748427727897621
1103	52-53	0.299556824548759
1103	54-55	0.1972307791799892
1103	56-57	0.2791022432803629
1103	58-59	0.27363189575847713
1103	60-61	0.27608265905684704
1103	62-63	0.3290197779133379
1103	64-65	0.37841464241423495
1103	66-67	0.39091958939720683
1103	68-69	0.40200385493305646
1103	70-71	0.3886202436705446
1103	72-73	0.3463323515788588
1103	74-75	0.3369806777697022
1103	76-77	0.33299246072093425
1103	78-79	0.31394376040592675
1103	80-81	0.3577623533359713
1103	82-83	0.32059822465309296
1103	84-85	0.34814741559713624
1103	86-87	0.3160969335835304
1103	88-89	0.34376617759748385
1103	90-91	0.3298123943732634
1103	92-93	0.3731561408474029
1103	94-95	0.35867846139617754
1103	96-97	0.33664430211796414
1103	98-99	0.36069867065101846
1103	100	0.41098456179173226
1104	1	2.3870820636013903
1104	2	3.2546428968967547
1104	3	1.5624409227317813
1104	4	1.1239849196050002
1104	5	0.6451404378955772
1104	6	0.6338477158653149
1104	7	0.3877594725182192
1104	8	0.4444680769582732
1104	9	0.46704029544980585
1104	10-11	0.4038683916403798
1104	12-13	0.3711562576217773
1104	14-15	0.45938500532228943
1104	16-17	0.4938672995569817
1104	18-19	0.4157784464424594
1104	20-21	0.45899423189326427
1104	22-23	0.42791748234161275
1104	24-25	0.46325475989957
1104	26-27	0.42292637757648066
1104	28-29	0.4480679617983583
1104	30-31	0.4079821742383558
1104	32-33	0.3535121497349678
1104	34-35	0.3894459118616851
1104	36-37	0.3998938150102802
1104	38-39	0.43013049325713837
1104	40-41	0.45240703657002257
1104	42-43	0.4596579718958367
1104	44-45	0.41671118934731055
1104	46-47	0.3948281772972422
1104	48-49	0.4628358258052998
1104	50-51	0.4773938106639122
1104	52-53	0.49373574677525767
1104	54-55	0.5138550813995266
1104	56-57	0.6065827547821314
1104	58-59	0.5552394748938241
1104	60-61	0.5449497155637459
1104	62-63	0.5105714407377846
1104	64-65	0.43916940899305246
1104	66-67	0.4948550674982428
1104	68-69	0.4989338439156228
1104	70-71	0.48793730937097024
1104	72-73	0.428695482954474
1104	74-75	0.4807006663405957
1104	76-77	0.47824632071614204
1104	78-79	0.5463800296866026
1104	80-81	0.5943275143413871
1104	82-83	0.5603343250362336
1104	84-85	0.5553100351329583
1104	86-87	0.5773098712035676
1104	88-89	0.5539428337537551
1104	90-91	0.5394891680414737
1104	92-93	0.5194485945082299
1104	94-95	0.5388321321299756
1104	96-97	0.5879357389908328
1104	98-99	0.6049146668044436
1104	100	0.6146648301450526
1105	1	1.3420720459373179
1105	2	2.683144483948631
1105	3	1.4367860901093543
1105	4	0.958392633016274
1105	5	0.6005771259601005
1105	6	0.5655329654940005
1105	7	0.2600477964828869
1105	8	0.3252142339733908
1105	9	0.44832283310994114
1105	10-11	0.3370928573147509
1105	12-13	0.3123516729696618
1105	14-15	0.3728925044731639
1105	16-17	0.42738588675604205
1105	18-19	0.2928229612000237
1105	20-21	0.3566409055279536
1105	22-23	0.29550470158643805
1105	24-25	0.3689800845235709
1105	26-27	0.3598257467665462
1105	28-29	0.33056160314197314
1105	30-31	0.3629834723282528
1105	32-33	0.3407849999971404
1105	34-35	0.35350678058635054
1105	36-37	0.3392454300034018
1105	38-39	0.26098206989444606
1105	40-41	0.22598637912295771
1105	42-43	0.33546735426075003
1105	44-45	0.3713508422450644
1105	46-47	0.4019811245138669
1105	48-49	0.32018261684754634
1105	50-51	0.3170652039502073
1105	52-53	0.28590301154975606
1105	54-55	0.2640394572628679
1105	56-57	0.2685228852556989
1105	58-59	0.21884842179551356
1105	60-61	0.17214883105654621
1105	62-63	-0.18672285076889494
1105	64-65	-0.09156917142711052
1105	66-67	0.04005251595473425
1105	68-69	0.17367620862801658
1105	70-71	0.21324391438351142
1105	72-73	0.13660329340957134
1105	74-75	0.10066785612423601
1105	76-77	0.09343326967345789
1105	78-79	0.011689984273573373
1105	80-81	0.06495245214182788
1105	82-83	0.0371634204736786
1105	84-85	0.06844324938061774
1105	86-87	0.08970570716186899
1105	88-89	0.06999630522959066
1105	90-91	0.09883816515971589
1105	92-93	0.08330657422123622
1105	94-95	0.08113429589683463
1105	96-97	0.06318127323618228
1105	98-99	0.08039060482018101
1105	100	0.11727171840468387
1106	1	1.894815106302545
1106	2	2.898175599626086
1106	3	1.2026020278034828
1106	4	0.8762977754323558
1106	5	0.5737524644830074
1106	6	0.5461783644720271
1106	7	0.2686942850633116
1106	8	0.3877163455074992
1106	9	0.4014003198404552
1106	10-11	0.2827579749966276
1106	12-13	0.22581824831522823
1106	14-15	0.20444599657514573
1106	16-17	0.3009798405525572
1106	18-19	0.11185760742715445
1106	20-21	0.2403767296646322
1106	22-23	0.2294437418899662
1106	24-25	0.2515061439253685
1106	26-27	0.1861243704905462
1106	28-29	0.23225670254861797
1106	30-31	0.23563032284108942
1106	32-33	0.23877543647702737
1106	34-35	0.29035119022949374
1106	36-37	0.32135635140918595
1106	38-39	0.2949072306421101
1106	40-41	0.22249361308873006
1106	42-43	0.20819090806838858
1106	44-45	0.17780010329725826
1106	46-47	0.1306907188944706
1106	48-49	0.13938279626583494
1106	50-51	0.25201384155839435
1106	52-53	0.21219593863411745
1106	54-55	0.1711065164829364
1106	56-57	0.20315284630335384
1106	58-59	0.25164184643997345
1106	60-61	0.05505763112535078
1106	62-63	0.2081639376616522
1106	64-65	0.015694484664010133
1106	66-67	-0.047434513984526916
1106	68-69	0.18489110310931522
1106	70-71	0.2998335480990022
1106	72-73	0.26048003807704134
1106	74-75	0.2669351453747524
1106	76-77	0.22832334216873917
1106	78-79	0.1637830403229117
1106	80-81	0.14665047061449243
1106	82-83	0.18515870112914357
1106	84-85	0.11745188908088622
1106	86-87	0.19227083620193142
1106	88-89	0.14955541930902783
1106	90-91	0.16341123332789564
1106	92-93	0.19375933257423483
1106	94-95	0.1720312698599571
1106	96-97	0.18382928021859257
1106	98-99	0.21468718499478
1106	100	0.23576499083766578
1107	1	1.7160540841067693
1107	2	0.9752423373341337
1107	3	0.5744509007971708
1107	4	0.44258035059264245
1107	5	0.2899811045492058
1107	6	0.39462767213581174
1107	7	0.1797638269757229
1107	8	0.2604395529790082
1107	9	0.3228475317102806
1107	10-11	0.24232378739464622
1107	12-13	0.17389376254542555
1107	14-15	0.20308364490642106
1107	16-17	0.25843756866323275
1107	18-19	0.17838787316414084
1107	20-21	0.21264499590614605
1107	22-23	0.17054172721216787
1107	24-25	0.1803036277370751
1107	26-27	0.2382881966711068
1107	28-29	0.2962471548339778
1107	30-31	0.2937011587454563
1107	32-33	0.19691155625810097
1107	34-35	0.19444431038682097
1107	36-37	0.17619728544656255
1107	38-39	0.28798558969708665
1107	40-41	0.2638554785405489
1107	42-43	0.23079626795011876
1107	44-45	0.1800215823728024
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2216	90-91	-1.5340136636406214
2216	92-93	-1.6113957616078345
2216	94-95	-1.595597185855329
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2216	98-99	-1.8201721712974148
2216	100	-1.8583700504707288
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	5898.0
3	153.0
4	286.0
5	643.0
6	1203.0
7	2027.0
8	3133.0
9	4865.0
10	8362.0
11	14144.0
12	15357.0
13	19611.0
14	20255.0
15	22928.0
16	24833.0
17	31741.0
18	44149.0
19	72570.0
20	124104.0
21	130241.0
22	67077.0
23	46660.0
24	43524.0
25	45505.0
26	50504.0
27	59315.0
28	73853.0
29	92213.0
30	112803.0
31	138483.0
32	173519.0
33	229185.0
34	323055.0
35	542671.0
36	793838.0
37	1241957.0
38	1498302.0
39	506399.0
40	2608.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.175206212794812	41.491197078046646	25.750473066810564	15.58312364234798
2	18.457793888547652	40.10794310276304	23.86162867414767	17.57263433454164
3	19.75556470406789	23.26901848201892	21.414638307897636	35.56077850601555
4	34.77569887191419	24.842159364927667	22.211077335763623	18.171064427394523
5	16.967546782813926	40.16461515038035	23.31105301994056	19.556785046865162
6	36.953466404428596	22.217526750149936	23.70452406204277	17.124482783378696
7	18.33903463953355	22.25503964502538	41.05366999576804	18.35225571967303
8	36.2737922159377	22.424284005978166	23.571298247382273	17.73062553070185
9	35.61846176077805	23.85256833132614	23.839696392244413	16.689273515651397
10-11	16.72364675391858	33.256111818291934	32.54484610898586	17.475395318803628
12-13	16.490578135250686	24.04354510202985	32.54801855623595	26.91785820648351
14-15	16.83196533562519	42.34723755740384	22.93277113722671	17.888025969744266
16-17	26.487058084928687	23.480382588030857	23.043495617924417	26.989063709116035
18-19	17.43003994854867	32.95214128046043	23.258303690937456	26.35951508005344
20-21	26.668077317852195	32.82399110864736	23.519582803453687	16.988348770046755
22-23	25.93512815927932	23.720076915907683	32.99742075484815	17.347374169964848
24-25	16.87093213420819	32.76336029887458	23.531697590466422	26.83400997645082
26-27	17.258248435653293	28.46160758767734	37.021887951152856	17.258256025516513
28-29	27.608706772644332	23.409951746595933	31.773870058405308	17.20747142235443
30-31	23.64587801737786	26.06090357475392	32.592697667033704	17.700520740834516
32-33	19.18520115783313	27.4719871675038	32.92604852063722	20.416763154025855
34-35	20.44345599781888	27.055963541530282	33.257325056674134	19.243255403976704
36-37	20.596742072120218	25.711939767323827	34.98792855453008	18.703389606025876
38-39	22.357576158695107	25.481372196903713	33.46480203946004	18.696249604941144
40-41	18.77398367146371	27.539207470233624	34.759988520551545	18.926820337751117
42-43	18.87389315072278	25.96599445689184	34.615263753061015	20.54484863932437
44-45	18.905657484462715	29.249231105538165	32.71465286654178	19.130458543457337
46-47	20.806226958031264	25.704176227557685	32.411340842380596	21.07825597203046
48-49	19.289913267810714	27.78933230280077	32.50415541609157	20.416599013296953
50-51	21.16574268489008	27.797854359471906	32.240196430959486	18.796206524678528
52-53	20.750012827686675	26.501423797317088	33.371947959365535	19.37661541563071
54-55	19.52382430624883	28.67689278229185	30.39793412193997	21.401348789519357
56-57	19.7592038532241	29.20504483334927	30.781733616890794	20.254017696535833
58-59	23.34207472255893	28.084208800542243	28.571386283128408	20.002330193770412
60-61	21.291831761536802	29.742217069982818	28.833251821714466	20.13269934676591
62-63	19.642549726330977	29.70495300398321	28.604432202435227	22.04806506725059
64-65	21.43555624813987	29.5592413489434	28.68713162233465	20.31807078058208
66-67	21.75146761976371	27.558006104715282	30.385585014467843	20.304941261053163
68-69	23.284844508578736	27.652056920568235	29.266530308766548	19.796568262086485
70-71	19.64235824090767	29.50156287914041	30.691040542867775	20.165038337084148
72-73	19.29663523844401	28.133861838522563	30.75434185734534	21.815161065688084
74-75	19.55274558725186	31.01238475601134	28.755309137708977	20.679560519027813
76-77	21.385854216622953	27.85700114589859	28.52836656854148	22.22877806893698
78-79	20.211521812371007	29.58554108124489	28.17551204078824	22.02742506559585
80-81	21.514592313245654	29.570693148540677	28.39675208246937	20.5179624557443
82-83	21.340532197633507	28.316247187453463	29.785404767625938	20.55781584728709
84-85	20.019229182698258	29.766184340739056	28.102301437779104	22.112285038783583
86-87	20.202819336933047	29.95030914101724	29.298936297460266	20.547935224589448
88-89	23.608756095748706	28.21485117276919	27.75557669029259	20.420816041189514
90-91	21.814210223289024	29.92384241518452	27.902991824884303	20.35895553664215
92-93	20.394619053260662	29.898564021865077	27.59114616636727	22.11567075850699
94-95	21.558937565963447	30.334571000992877	27.510428542869846	20.59606289017383
96-97	21.689992899859174	28.179377411382006	29.13437101897836	20.996258669780456
98-99	23.351208202569605	28.687225916078507	27.265645937328586	20.6959199440233
100	20.25015312012131	28.526229268066842	30.202740907093467	21.02087670471838
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	77977.0
1	45008.5
2	8761.0
3	4598.0
4	3397.5
5	2864.5
6	2765.5
7	2937.0
8	2784.5
9	2706.5
10	2940.0
11	4828.5
12	32319.0
13	35781.5
14	12546.0
15	12669.5
16	14923.0
17	17357.0
18	19901.5
19	22801.5
20	26372.5
21	30537.0
22	35573.5
23	42592.0
24	50464.5
25	58205.0
26	69749.5
27	82392.0
28	91071.5
29	98810.5
30	107682.5
31	117208.5
32	128245.0
33	140436.0
34	154205.0
35	165544.5
36	172951.0
37	178637.5
38	181137.0
39	180225.0
40	179849.0
41	184014.5
42	185510.5
43	185026.0
44	188069.0
45	192864.5
46	208850.0
47	238437.0
48	253172.0
49	283966.5
50	409709.5
51	389801.0
52	221169.0
53	152597.0
54	134526.5
55	126341.5
56	115052.5
57	101379.0
58	96758.0
59	83180.0
60	60850.0
61	47480.5
62	36803.0
63	27879.5
64	20574.0
65	14699.5
66	11314.5
67	9425.0
68	7340.0
69	5223.0
70	3580.0
71	2542.0
72	1959.5
73	1561.5
74	1106.0
75	739.5
76	519.5
77	315.5
78	223.5
79	183.0
80	127.0
81	77.0
82	62.0
83	46.0
84	30.5
85	25.5
86	21.0
87	20.0
88	15.0
89	7.0
90	7.5
91	10.0
92	6.0
93	2.5
94	2.0
95	1.5
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025728698990008157
2	0.012143338756346033
3	0.0057984442561552305
4	0.0
5	0.0037188974941309728
6	0.0010170046208439801
7	1.517917344543254E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.107504067259525E-4
26-27	0.00364300162690381
28-29	6.830628050444644E-4
30-31	7.893170191624921E-4
32-33	0.004910462609597427
34-35	0.00809808903313826
36-37	0.00486492508926113
38-39	0.005282352359010524
40-41	0.008340955808265181
42-43	0.010549525544575616
44-45	0.00901642902658693
46-47	0.012279951317354928
48-49	0.016522530295353322
50-51	0.05888760338155554
52-53	0.010018254473985477
54-55	0.005517629547414729
56-57	0.0037037183206855397
58-59	0.00818157448708814
60-61	0.014852821216355741
62-63	0.012446922225254684
64-65	0.011073207028443038
66-67	0.009213758281377552
68-69	0.006193102765736476
70-71	0.004439908232789018
72-73	0.0032103951837089825
74-75	0.0023907198176556254
76-77	0.0019884717213516628
78-79	0.0014496110640388076
80-81	0.0018518591603427698
82-83	0.001259871395970901
84-85	0.0010701317279029942
86-87	6.375252847081667E-4
88-89	0.0012674609826936172
90-91	8.348545394987897E-4
92-93	3.8706892285852974E-4
94-95	6.830628050444644E-4
96-97	0.0011612067685755894
98-99	0.0035443369995084985
100	1.366125610088929E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6587974.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.08092199832282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.69980935552455	45.551817483878146
2	11.69213315742085	12.880269488758032
3	3.084161343637623	5.096353511974392
4	1.0117964582235859	2.2292272717437047
5	0.40765580903851645	1.1227028909906855
6	0.22213174064255056	0.73411326478104
7	0.14903904844080784	0.5746445741310674
8	0.10628115306258244	0.4683251121385529
9	0.08309361750155622	0.4119185757745521
>10	0.40073961129861757	3.9986021449791203
>50	0.08079301692277485	3.3360364140395857
>100	0.05566286483203274	5.037801177429668
>500	0.0029393639457776315	1.1056286900477603
>1k	0.002472345662414238	3.114778671227012
>5k	9.88938264845639E-4	3.605764773326895
>10k+	3.021755809250564E-4	10.732015954779785
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	363117	5.511815923985128	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG	142755	2.1669029052027224	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	73392	1.1140298975071852	No Hit
TACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	31446	0.4773242881650717	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGTAACGAGTGGTATCAACGCAG	20931	0.3177152793863485	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGGAGGCATTCAGGCAGCGAGA	15395	0.23368337519243396	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15005	0.22776349754871525	No Hit
AATGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14033	0.21300934095975485	No Hit
ATCGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13150	0.19960613080743794	No Hit
GTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTT	10033	0.15229264717802468	No Hit
TACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAG	10016	0.15203460122945234	No Hit
ACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACT	9658	0.1466004571359875	No Hit
ACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTC	9333	0.14166722576622193	No Hit
CCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGAT	8869	0.13462408928754122	No Hit
ACGAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAGA	8033	0.12193430028715961	No Hit
ACGAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7739	0.11747162329420244	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGGTAACGAGTGGTATCAACGCA	7603	0.11540725570562362	No Hit
ATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTA	7402	0.11235624184309168	No Hit
TGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTC	7223	0.10963916979635925	No Hit
CACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCT	7161	0.10869806104274243	No Hit
GCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCC	7148	0.1085007317879518	No Hit
CCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATA	6998	0.10622385577113691	No Hit
TCGTTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCG	6928	0.10516131362995665	No Hit
AACGAGTGGTATCAACGCAGAGTACATGGAAACGAGTGGTATCAACGCAG	6870	0.10428092157012156	No Hit
TTCCCATGTACTCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTG	6696	0.10163974539061629	No Hit
GAGTGGTATCAACGCAGAGTACATGGGAACGAGTGGTATCAACGCAGAGT	6694	0.10160938704372542	No Hit
TCTGCGTTGATACCACTCGTTCCCATGTACTCTGCGTTGATACCACTCGT	6614	0.10039505316809083	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.071669378173016E-4	1.5179173445432541E-5	0.0	0.0	0.0
2	6.527044581535993E-4	1.5179173445432541E-5	0.0	0.0	6.0716693781730165E-5
3	6.527044581535993E-4	1.5179173445432541E-5	0.0	0.0	6.0716693781730165E-5
4	6.527044581535993E-4	1.5179173445432541E-5	0.0	0.0	7.58958672271627E-5
5	6.527044581535993E-4	1.5179173445432541E-5	0.0	0.0	7.58958672271627E-5
6	6.527044581535993E-4	1.5179173445432541E-5	0.0	0.0	9.107504067259525E-5
7	6.527044581535993E-4	3.0358346890865082E-5	0.0	0.0	1.0625421411802778E-4
8	6.678836315990318E-4	4.5537520336297624E-5	0.0	0.0	1.0625421411802778E-4
9	6.830628050444644E-4	6.0716693781730165E-5	0.0	0.0	1.2143338756346033E-4
10-11	7.134211519353294E-4	7.58958672271627E-5	0.0	0.0	1.3661256100889286E-4
12-13	7.134211519353294E-4	9.107504067259525E-5	0.0	0.0	1.5938132117704167E-4
14-15	7.28600325380762E-4	1.1384380084074406E-4	0.0	0.0	1.8973966806790676E-4
16-17	8.044961926079246E-4	1.3661256100889286E-4	0.0	1.5179173445432541E-5	2.049188415133393E-4
18-19	8.728024731123711E-4	1.517917344543254E-4	0.0	1.5179173445432541E-5	2.2768760168148812E-4
20-21	9.638775137849664E-4	1.5938132117704167E-4	0.0	1.5179173445432541E-5	2.4286677512692066E-4
22-23	0.0011763859420210218	1.7456049462247423E-4	0.0	1.5179173445432541E-5	3.035834689086508E-4
24-25	0.0012978193295844823	2.049188415133393E-4	0.0	1.5179173445432541E-5	3.035834689086508E-4
26-27	0.0015103277578205378	2.2768760168148812E-4	0.0	1.5179173445432541E-5	3.415314025222322E-4
28-29	0.0016697090789975794	2.2768760168148812E-4	0.0	1.5179173445432541E-5	3.4912098924494845E-4
30-31	0.0018366799868973376	2.7322512201778573E-4	0.0	1.5179173445432541E-5	3.4912098924494845E-4
32-33	0.0022009801495877185	2.8840429546321826E-4	0.0	1.5179173445432541E-5	3.4912098924494845E-4
34-35	0.0026184074193371133	2.9599388218593453E-4	0.0	1.5179173445432541E-5	3.4912098924494845E-4
36-37	0.0030130659289183594	3.339418157995159E-4	0.0	1.5179173445432541E-5	3.4912098924494845E-4
38-39	0.0036885391472401075	3.339418157995159E-4	0.0	1.5179173445432541E-5	3.4912098924494845E-4
40-41	0.00472072294152952	3.415314025222322E-4	0.0	1.5179173445432541E-5	3.4912098924494845E-4
42-43	0.005775675495987082	3.794793361358135E-4	0.0	1.5179173445432541E-5	3.7188974941309726E-4
44-45	0.007384667881202931	4.1742726974939486E-4	0.0	3.0358346890865082E-5	3.794793361358135E-4
46-47	0.009084735307091377	4.5537520336297625E-4	0.0	3.794793361358135E-5	3.9465850958124606E-4
48-49	0.011308484216847242	4.7814396353112505E-4	0.0	4.5537520336297624E-5	4.250168564721111E-4
50-51	0.014010377090134236	4.933231369765576E-4	0.0	4.5537520336297624E-5	4.326064431948274E-4
52-53	0.017327026487961243	5.236814838674227E-4	0.0	4.5537520336297624E-5	4.705543768084088E-4
54-55	0.021675859680077667	5.312710705901389E-4	0.0	4.5537520336297624E-5	4.857335502538413E-4
56-57	0.027246616334551412	5.61629417481004E-4	0.0	4.5537520336297624E-5	4.857335502538413E-4
58-59	0.03441118620079557	5.843981776491528E-4	0.0	4.5537520336297624E-5	4.857335502538413E-4
60-61	0.04387540084402276	5.919877643718691E-4	0.0	4.5537520336297624E-5	4.857335502538413E-4
62-63	0.05575310406507372	5.995773510945853E-4	0.0	4.5537520336297624E-5	4.857335502538413E-4
64-65	0.07242742609488137	6.45114871430883E-4	0.0	4.5537520336297624E-5	4.857335502538413E-4
66-67	0.09396667321395014	6.527044581535993E-4	0.0	5.31271070590139E-5	4.857335502538413E-4
68-69	0.12134990210951045	6.527044581535993E-4	0.0	6.0716693781730165E-5	4.933231369765576E-4
70-71	0.1587741542392244	6.830628050444644E-4	0.0	6.0716693781730165E-5	5.009127236992739E-4
72-73	0.2077573469476352	6.830628050444644E-4	0.0	6.0716693781730165E-5	5.009127236992739E-4
74-75	0.26948345576348665	7.210107386580458E-4	0.0	6.0716693781730165E-5	5.009127236992739E-4
76-77	0.3450226124146817	7.58958672271627E-4	0.0	6.0716693781730165E-5	5.009127236992739E-4
78-79	0.439277689924095	7.741378457170596E-4	0.0	6.0716693781730165E-5	5.009127236992739E-4
80-81	0.5603771356717558	8.12085779330641E-4	0.0	6.0716693781730165E-5	5.160918971447064E-4
82-83	0.7079414703215283	8.500337129442223E-4	0.0	6.0716693781730165E-5	5.160918971447064E-4
84-85	0.882380531556439	8.652128863896549E-4	0.0	6.830628050444643E-5	5.160918971447064E-4
86-87	1.0842938967275826	8.728024731123711E-4	0.0	7.58958672271627E-5	5.160918971447064E-4
88	1.2568659196287053	8.9557123328052E-4	0.0	7.58958672271627E-5	5.160918971447064E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	9280	0.0	87.84491	1
ATCGAGT	3895	0.0	82.558945	1
TCGAGTG	4935	0.0	75.336006	2
TGAGTGG	7990	0.0	56.70316	3
ATGAGTG	7605	0.0	56.55034	2
GACGAGT	2690	0.0	55.925926	1
AATGAGT	7535	0.0	52.721645	1
AGTGGTA	241280	0.0	46.94	5
GTGGTAT	244390	0.0	46.31084	6
TGGTATC	245385	0.0	46.220375	7
GTATCAA	248675	0.0	45.78273	9
GGTATCA	247895	0.0	45.778923	8
GAGTGGT	258705	0.0	43.783817	4
CGAGTGG	253620	0.0	43.078487	3
ACGAGTG	247050	0.0	42.715305	2
TTCGAGT	1105	0.0	41.69455	1
AACGAGT	235030	0.0	40.99587	1
AGTACTT	65290	0.0	39.57404	20-21
GAGTACT	64435	0.0	39.34793	20-21
ACGATTG	1210	0.0	39.2328	2
>>END_MODULE