FastQCFastQC Report
Thu 26 May 2016
SRR2132629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132629_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5006780
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4480968.949784092770203No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1328432.6532621764886812No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT284140.5675104558219055No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT156080.3117372842425671No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152210.3040077654700226No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT132890.26542009035747527No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG122320.24430871737923376No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA98540.1968131214073716No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA68470.13675456081553414No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57660.11516383783589453No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGGTTAAGCGACTAAGCGTAC52740.10533716280723339No Hit
GACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT50640.10114285029499998No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT68050.088.918481
AACGAGT1025150.084.335871
ACGAGTG1111250.083.722452
CGAGTGG1155950.083.0334243
GTGGAAT1200800.082.473436
TGGAATC1210150.082.018727
GAATCAC1214700.081.966839
GGAATCA1212800.081.835638
GAGTGGA1211950.081.8201754
AGTGGAA1218300.081.3937155
ATCGAGT34750.080.636761
TCGAGTG39700.075.6537552
ATGAGTG56250.057.4056132
TGAGTGG59500.056.3966943
GACGAGT18100.054.8081931
AATGAGT55650.053.9855231
CGATTGT8050.046.121523
ACGATTG12150.045.261582
AGTACTT670350.043.31395320-21
GAGTACT664850.043.2340320-21
Produced by FastQC (version 0.11.5)