FastQCFastQC Report
Thu 26 May 2016
SRR2132627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132627_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9515400
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT4503244.732580868907245No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG821100.8629169556718582No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT324600.3411312188662589No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA174460.1833448935409967No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT165970.17442251508081635No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT135900.14282111104104925No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT95650.1005212602728209No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT92050.086.924441
ATCGAGT43250.073.370891
TCGAGTG50500.069.430592
GTGGAAT1934850.062.588266
TGGAATC1941000.062.5352137
GAATCAC1957950.062.2506569
GGAATCA1955400.062.089118
AGTGGAA1958850.061.8363465
ACGAGTG1884100.060.3516542
CGAGTGG1943950.060.250113
AACGAGT1758250.059.625271
GAGTGGA2038050.059.461014
GACGAGT28700.050.6154021
ATGAGTG96300.042.0711482
TGAGTGG102050.041.953233
GAGTACT773550.040.89181520-21
AGTACTT790400.040.73057620-21
GTACTTT780550.039.73950222-23
CATGGGG364850.038.11977424-25
AATGAGT101850.037.0185551