##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132627_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	9515400
Sequences flagged as poor quality	0
Sequence length	100
%GC	41
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.542172478298337	31.0	19.0	33.0	16.0	34.0
2	27.877342413350988	31.0	25.0	34.0	16.0	34.0
3	28.08649862328436	31.0	25.0	34.0	16.0	34.0
4	33.89353553187465	35.0	33.0	37.0	28.0	37.0
5	34.969769321310714	35.0	35.0	37.0	32.0	37.0
6	35.3661023183471	37.0	35.0	37.0	33.0	37.0
7	35.65465949933792	37.0	35.0	37.0	35.0	37.0
8	35.74687716753893	37.0	35.0	37.0	35.0	37.0
9	37.52172530844736	39.0	37.0	39.0	35.0	39.0
10-11	37.71952445509385	39.0	37.0	39.0	35.0	39.0
12-13	37.72732381192593	39.0	37.0	39.0	35.0	39.0
14-15	39.15000514954705	40.0	39.0	41.0	36.0	41.0
16-17	39.11667181621372	40.5	39.0	41.0	36.0	41.0
18-19	39.19513288984173	40.5	39.0	41.0	36.0	41.0
20-21	39.18499632175211	40.5	39.0	41.0	36.0	41.0
22-23	39.13100347857158	40.0	39.0	41.0	36.0	41.0
24-25	39.01902542194758	40.0	39.0	41.0	35.0	41.0
26-27	39.039301500725145	40.0	39.0	41.0	36.0	41.0
28-29	38.99155122222923	40.0	39.0	41.0	35.0	41.0
30-31	38.90420823086785	40.0	39.0	41.0	35.0	41.0
32-33	38.82300360468294	40.0	38.5	41.0	35.0	41.0
34-35	38.752018622443615	40.0	38.0	41.0	35.0	41.0
36-37	38.620483531958726	40.0	38.0	41.0	35.0	41.0
38-39	38.30881586691048	40.0	37.5	41.0	34.5	41.0
40-41	38.15443538894844	40.0	37.0	41.0	33.5	41.0
42-43	38.046836023708934	40.0	37.0	41.0	33.0	41.0
44-45	37.92549524980558	40.0	37.0	41.0	33.0	41.0
46-47	37.819295352796516	40.0	37.0	41.0	33.0	41.0
48-49	37.693586344241965	40.0	37.0	41.0	33.0	41.0
50-51	37.50073817180571	40.0	36.5	41.0	33.0	41.0
52-53	37.09125733022259	40.0	36.0	41.0	32.0	41.0
54-55	36.44796424743048	40.0	36.0	41.0	30.5	41.0
56-57	35.901982470521475	40.0	36.0	41.0	28.0	41.0
58-59	35.65932730100679	40.0	35.0	41.0	27.5	41.0
60-61	35.39121818315573	39.0	35.0	41.0	26.5	41.0
62-63	35.148286567038696	39.0	35.0	41.0	26.0	41.0
64-65	34.89167481135843	39.0	35.0	41.0	26.0	41.0
66-67	34.56036708913971	38.0	35.0	40.0	25.5	41.0
68-69	34.2446395317065	37.0	34.0	40.0	25.0	41.0
70-71	33.94670392206318	37.0	34.0	40.0	25.0	41.0
72-73	33.61552693528385	36.5	34.0	39.0	24.0	41.0
74-75	33.31346233474157	36.0	34.0	39.0	24.0	41.0
76-77	33.054370651785526	35.5	34.0	38.5	24.0	40.0
78-79	32.71958871933917	35.0	34.0	37.0	23.5	39.5
80-81	32.38272484603905	35.0	34.0	37.0	22.5	39.0
82-83	32.028603789646255	35.0	33.0	36.5	21.0	39.0
84-85	31.711559261828192	35.0	33.0	36.0	20.0	37.0
86-87	31.536263951068793	35.0	33.0	36.0	20.0	37.0
88-89	31.374587720957607	35.0	33.0	35.5	20.0	37.0
90-91	31.184021323328498	35.0	33.0	35.0	18.5	36.0
92-93	31.02852034596549	35.0	33.0	35.0	17.0	36.0
94-95	30.856539872207158	35.0	33.0	35.0	9.0	36.0
96-97	30.68511160855035	35.0	33.0	35.0	2.0	36.0
98-99	30.544271444185213	35.0	33.0	35.0	2.0	36.0
100	30.464547155137986	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.9729353395697906
1101	2	4.099366593647151
1101	3	3.517969053658323
1101	4	1.9640572719867961
1101	5	1.0828960651838244
1101	6	0.8438216320637366
1101	7	0.4872518068833074
1101	8	0.5320448132026527
1101	9	0.6112831626085224
1101	10-11	0.4331383491014691
1101	12-13	0.39973419569653146
1101	14-15	0.5250666731720912
1101	16-17	0.49008186938204545
1101	18-19	0.46377013661783906
1101	20-21	0.4855540708335795
1101	22-23	0.4530787227465467
1101	24-25	0.49127891943126656
1101	26-27	0.41996626057810715
1101	28-29	0.4604499248599794
1101	30-31	0.502960667317403
1101	32-33	0.4501043027231333
1101	34-35	0.44668062311213674
1101	36-37	0.3935032318095608
1101	38-39	0.43988621751426393
1101	40-41	0.43640573768269064
1101	42-43	0.47092341803534055
1101	44-45	0.4716078665158818
1101	46-47	0.4635025059719666
1101	48-49	0.49058966149028294
1101	50-51	0.5550434590969999
1101	52-53	0.5607543311762413
1101	54-55	0.4507527775981188
1101	56-57	0.4655657016642749
1101	58-59	0.41444629625026863
1101	60-61	0.4424480264341426
1101	62-63	0.4471559356047692
1101	64-65	0.40941047096542604
1101	66-67	0.40812672375208336
1101	68-69	0.39869513033312387
1101	70-71	0.397972005914049
1101	72-73	0.3752943009144829
1101	74-75	0.3643384378970609
1101	76-77	0.35919807525444014
1101	78-79	0.3599244433474098
1101	80-81	0.3169765736717878
1101	82-83	0.32782620616063696
1101	84-85	0.2935303263489111
1101	86-87	0.2904078718237173
1101	88-89	0.29209810195875363
1101	90-91	0.3114020676793885
1101	92-93	0.30646968872893865
1101	94-95	0.3358010280762542
1101	96-97	0.3331828445554308
1101	98-99	0.3670548874042616
1101	100	0.35821632649401636
1102	1	3.0178567505698695
1102	2	3.5708615491358735
1102	3	4.300779529388485
1102	4	2.1703433193420665
1102	5	1.1337632182705661
1102	6	0.8863615712193962
1102	7	0.4863404074612845
1102	8	0.5149339776881092
1102	9	0.618331555549446
1102	10-11	0.42279853295598
1102	12-13	0.3762765264068335
1102	14-15	0.483369988718934
1102	16-17	0.500403364863125
1102	18-19	0.4462444796428926
1102	20-21	0.48733928442699437
1102	22-23	0.39072353697951456
1102	24-25	0.4791517336891218
1102	26-27	0.4205393605653853
1102	28-29	0.4269538722766697
1102	30-31	0.42205705310512087
1102	32-33	0.4191138906090117
1102	34-35	0.4017929465907102
1102	36-37	0.4416282598322425
1102	38-39	0.4721365829085471
1102	40-41	0.43835540820033714
1102	42-43	0.43033630295432346
1102	44-45	0.46398910881568156
1102	46-47	0.4555742384525061
1102	48-49	0.477188138386623
1102	50-51	0.4842321389858597
1102	52-53	0.4544386360947428
1102	54-55	0.415343352581651
1102	56-57	0.4091414126680206
1102	58-59	0.37758517310773243
1102	60-61	0.38281505756189205
1102	62-63	0.32652464712788287
1102	64-65	0.3018603728511806
1102	66-67	0.2990271136132492
1102	68-69	0.3195200193204073
1102	70-71	0.33384581901280797
1102	72-73	0.3185189480861581
1102	74-75	0.3227598509351566
1102	76-77	0.326516037250002
1102	78-79	0.33791881542808255
1102	80-81	0.32915979404557305
1102	82-83	0.353177189544823
1102	84-85	0.33883005692359447
1102	86-87	0.3587134763809736
1102	88-89	0.3566485602649756
1102	90-91	0.3273898507567168
1102	92-93	0.37923299625673224
1102	94-95	0.3981692464002222
1102	96-97	0.39184030240664214
1102	98-99	0.4164443845149002
1102	100	0.42808913088359546
1103	1	2.7146533308009815
1103	2	3.3423985338742135
1103	3	3.131185283609984
1103	4	1.751748484465523
1103	5	0.9133565804653827
1103	6	0.7301481672083767
1103	7	0.3765001121491096
1103	8	0.4578466329263904
1103	9	0.5157522053185488
1103	10-11	0.3426421513203408
1103	12-13	0.3282715535758669
1103	14-15	0.3963281026687753
1103	16-17	0.4529856519216793
1103	18-19	0.37358709092890763
1103	20-21	0.37998656665076425
1103	22-23	0.3582414815246864
1103	24-25	0.36506109237005546
1103	26-27	0.3068938134362895
1103	28-29	0.3174597042881828
1103	30-31	0.3241836480402256
1103	32-33	0.3163880032423094
1103	34-35	0.2882505602868264
1103	36-37	0.2818111548943989
1103	38-39	0.337983742960283
1103	40-41	0.31293215745834857
1103	42-43	0.30745515755265984
1103	44-45	0.29723592053007764
1103	46-47	0.2940020430521386
1103	48-49	0.2984096169834274
1103	50-51	0.33936934144666253
1103	52-53	0.27903016034252204
1103	54-55	0.2338127997071595
1103	56-57	0.2909992358972815
1103	58-59	0.21639322716529819
1103	60-61	0.2063907859448122
1103	62-63	0.21582344931209008
1103	64-65	0.2744920907335171
1103	66-67	0.25347198325211906
1103	68-69	0.2697052275040974
1103	70-71	0.2885400422221025
1103	72-73	0.24500482758639208
1103	74-75	0.2554222698644537
1103	76-77	0.24400488184749491
1103	78-79	0.19745853805041946
1103	80-81	0.20226905489422364
1103	82-83	0.2272679656621719
1103	84-85	0.223958734978023
1103	86-87	0.24970412793034313
1103	88-89	0.24684076021130252
1103	90-91	0.23627503478235212
1103	92-93	0.22614120763108048
1103	94-95	0.2617819897982656
1103	96-97	0.21988942568752634
1103	98-99	0.23705464589213676
1103	100	0.25812692384113234
1104	1	2.4925947959744903
1104	2	3.282187433250023
1104	3	1.690298048637782
1104	4	1.1290188045944802
1104	5	0.6143452547108978
1104	6	0.589495177717744
1104	7	0.3222171199494568
1104	8	0.40974284733840705
1104	9	0.45743501507192974
1104	10-11	0.3368474749938315
1104	12-13	0.31054142952499575
1104	14-15	0.42030548548279256
1104	16-17	0.4482930225872579
1104	18-19	0.36169255763067554
1104	20-21	0.3884745137596397
1104	22-23	0.35608348307504656
1104	24-25	0.40657642316585907
1104	26-27	0.3642409784584828
1104	28-29	0.37531403895489035
1104	30-31	0.33163507655939384
1104	32-33	0.3046463052188031
1104	34-35	0.35710012738369556
1104	36-37	0.36490087221432077
1104	38-39	0.3377422350196255
1104	40-41	0.32359588802503936
1104	42-43	0.3075838390641934
1104	44-45	0.2461022851069501
1104	46-47	0.26551746707820456
1104	48-49	0.2728939051905783
1104	50-51	0.2998687789178405
1104	52-53	0.3329781619667429
1104	54-55	0.2769666523199774
1104	56-57	0.27946953279587206
1104	58-59	0.2328819487385374
1104	60-61	0.21600324141251548
1104	62-63	0.2468092536095412
1104	64-65	0.2526116019504272
1104	66-67	0.2551263304603708
1104	68-69	0.2738273559378612
1104	70-71	0.22314910992782444
1104	72-73	0.14964173254129065
1104	74-75	0.21816710173109755
1104	76-77	0.21263423550763605
1104	78-79	0.1957469165838006
1104	80-81	0.23071124672932086
1104	82-83	0.2288707096246796
1104	84-85	0.19746961316787903
1104	86-87	0.18898312607440815
1104	88-89	0.1849010250826275
1104	90-91	0.20982109700339535
1104	92-93	0.21494694047258633
1104	94-95	0.21977182603723833
1104	96-97	0.20093621860815247
1104	98-99	0.24127641787752552
1104	100	0.24856274620862706
1105	1	1.461274076883754
1105	2	2.754413413066107
1105	3	1.518717975721671
1105	4	0.9935610686011458
1105	5	0.6033378531903821
1105	6	0.5701695202092765
1105	7	0.2433006033516918
1105	8	0.33895435853932554
1105	9	0.4346369885602286
1105	10-11	0.2561362963399745
1105	12-13	0.2621783783212024
1105	14-15	0.33710953917505293
1105	16-17	0.37200476675899097
1105	18-19	0.306818535661904
1105	20-21	0.33320882134311347
1105	22-23	0.28646712871139357
1105	24-25	0.35196596117908996
1105	26-27	0.30593991346243854
1105	28-29	0.2736990133703756
1105	30-31	0.30089308563586314
1105	32-33	0.2799022560732354
1105	34-35	0.25210291504863847
1105	36-37	0.25984594558602936
1105	38-39	0.2055338783187537
1105	40-41	0.1386873361171581
1105	42-43	0.25726114394878863
1105	44-45	0.28949151499747927
1105	46-47	0.2933364609636655
1105	48-49	0.26046237559624075
1105	50-51	0.26326611581712456
1105	52-53	0.23306209721964422
1105	54-55	0.26767723445581026
1105	56-57	0.26365440735330026
1105	58-59	0.266955575032668
1105	60-61	0.22984445582927293
1105	62-63	-0.07994312377311985
1105	64-65	0.07140838986525466
1105	66-67	0.1695468366834163
1105	68-69	0.23775104953990223
1105	70-71	0.26066181529206744
1105	72-73	0.17260233369994893
1105	74-75	0.17174948603712892
1105	76-77	0.19799870097581618
1105	78-79	0.15922650956123618
1105	80-81	0.16356140046188017
1105	82-83	0.17025964313499742
1105	84-85	0.16723143338392177
1105	86-87	0.1796927688117762
1105	88-89	0.17868784363124135
1105	90-91	0.17185718681364648
1105	92-93	0.17873330576143687
1105	94-95	0.14220352237480327
1105	96-97	0.13800162939300975
1105	98-99	0.1477679900641924
1105	100	0.17902025204933736
1106	1	1.8671674016673343
1106	2	2.8778353758702337
1106	3	1.2486913022158248
1106	4	0.8319595946261629
1106	5	0.5281641935469708
1106	6	0.5142385391122417
1106	7	0.2732655622376896
1106	8	0.3474854519664916
1106	9	0.37263156670235986
1106	10-11	0.2713655570906539
1106	12-13	0.2048898975495561
1106	14-15	0.19548183993817503
1106	16-17	0.2927771637799239
1106	18-19	0.06167499226386752
1106	20-21	0.20544832265726853
1106	22-23	0.19674086687558656
1106	24-25	0.2651364821345936
1106	26-27	0.21013821818320366
1106	28-29	0.2336101312293266
1106	30-31	0.23235962729818027
1106	32-33	0.19731562703092465
1106	34-35	0.2196972092619447
1106	36-37	0.24671298333711178
1106	38-39	0.27424596345094443
1106	40-41	0.1800141889804081
1106	42-43	0.18451199108500305
1106	44-45	0.16302636325693243
1106	46-47	0.16403684945394303
1106	48-49	0.13933874712223115
1106	50-51	0.28403180413128126
1106	52-53	0.2538258147057988
1106	54-55	0.24266242853264686
1106	56-57	0.2589925248463061
1106	58-59	0.24545706060085593
1106	60-61	0.07947302556986813
1106	62-63	0.1726841747688752
1106	64-65	0.039352004513808936
1106	66-67	-0.08069235620964577
1106	68-69	0.247850461011204
1106	70-71	0.3470463025589652
1106	72-73	0.3765573684680916
1106	74-75	0.3888630352856026
1106	76-77	0.30687777866253896
1106	78-79	0.29557678832146195
1106	80-81	0.3030343969866749
1106	82-83	0.31872705418789593
1106	84-85	0.2990678431804419
1106	86-87	0.33510879394520643
1106	88-89	0.27257212887269233
1106	90-91	0.31143847711759776
1106	92-93	0.31047229352224903
1106	94-95	0.3244794160142561
1106	96-97	0.3433798844075362
1106	98-99	0.41748864320181767
1106	100	0.4171027278738215
1107	1	1.6100955697991388
1107	2	0.8768548280240402
1107	3	0.5923746938058301
1107	4	0.38661610854499173
1107	5	0.22155373953543034
1107	6	0.3479483983521945
1107	7	0.16072454889580712
1107	8	0.20622665858982714
1107	9	0.28657123991344235
1107	10-11	0.15315624488908952
1107	12-13	0.14753626293406086
1107	14-15	0.20217524876520088
1107	16-17	0.21184046757219477
1107	18-19	0.1819987004276271
1107	20-21	0.20135220795975783
1107	22-23	0.1353595745200593
1107	24-25	0.1244854249098637
1107	26-27	0.17276660779118913
1107	28-29	0.222917640712339
1107	30-31	0.2207110273145929
1107	32-33	0.14575858644774087
1107	34-35	0.19453557371395647
1107	36-37	0.16527624417470577
1107	38-39	0.18617506589149713
1107	40-41	0.23691554292604167
1107	42-43	0.23334621907159914
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2216	68-69	-1.0694918076131898
2216	70-71	-1.032493135241289
2216	72-73	-1.06915959488245
2216	74-75	-1.122557535478748
2216	76-77	-1.0373222751512792
2216	78-79	-1.0541155757978196
2216	80-81	-0.9860215559979189
2216	82-83	-0.9574750451936183
2216	84-85	-0.963317359108327
2216	86-87	-0.9876168996939931
2216	88-89	-1.0117559879827063
2216	90-91	-1.0680946852294468
2216	92-93	-1.0625944914484258
2216	94-95	-1.1045068780764105
2216	96-97	-1.138394320670514
2216	98-99	-1.2177350781749077
2216	100	-1.2073241955450271
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4896.0
3	118.0
4	213.0
5	352.0
6	650.0
7	1177.0
8	2036.0
9	3460.0
10	5849.0
11	10574.0
12	11201.0
13	14865.0
14	16482.0
15	18536.0
16	22829.0
17	29656.0
18	43756.0
19	74284.0
20	142434.0
21	177398.0
22	82822.0
23	50460.0
24	46577.0
25	50037.0
26	57048.0
27	67515.0
28	86435.0
29	108849.0
30	135597.0
31	169681.0
32	217973.0
33	285552.0
34	393499.0
35	592954.0
36	999678.0
37	1879777.0
38	2690509.0
39	1013756.0
40	5915.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	16.75022973888082	37.90446604815697	27.916338255523453	17.42896595743876
2	19.35673839803563	35.54844952237129	27.29567743019083	17.799134649402255
3	20.378819917972553	25.402244320044932	22.191131686170184	32.02780407581233
4	29.908169914034094	26.48539210122538	24.456365470710637	19.15007251402989
5	17.916705727650424	36.11987385018827	25.27095010465541	20.692470317505894
6	32.65755859956506	23.469262822058916	25.95706641334835	17.916112165027677
7	19.561072382554	23.614544590106977	37.627216505333	19.19716652200602
8	31.66328267860521	23.554837421443136	25.923481934548203	18.85839796540345
9	31.546177774975305	25.20474178699792	25.607678079744417	17.641402358282363
10-11	17.788826533829372	38.538952645185695	25.443622969081698	18.22859785190323
12-13	17.724078861634823	24.95557727473359	32.04329297769931	25.27705088593228
14-15	17.937926939487568	31.599118271433674	25.247325388317886	25.215629400760868
16-17	24.90862706770078	24.706959245013348	31.960358996994348	18.424054690291527
18-19	18.220742165331988	24.793981335519263	31.969454778569478	25.01582172057927
20-21	25.060533451037266	31.61177669882506	25.215787039956282	18.11190281018139
22-23	24.7615864808626	24.884807785274397	32.03456502091347	18.319040712949533
24-25	18.15890008377555	31.44643843054346	25.1784164808564	25.216245004824593
26-27	18.366409860693565	27.732606847660655	35.52416884002631	18.376814451619467
28-29	24.633419620304746	24.642462911078912	32.39096042953371	18.33315703908262
30-31	22.819081799408323	25.78762210533506	32.66563500028901	18.727661094967605
32-33	19.825318396287763	26.95711883394817	33.22068208411608	19.996880685647998
34-35	20.21220114824531	26.625862378199745	33.46232625854285	19.69961021501209
36-37	20.137844875591686	26.238314575820226	34.09896595323639	19.5248745953517
38-39	20.844056041217236	26.21320306123457	33.436146261413995	19.506594636134196
40-41	19.68275662664886	27.63943858024281	33.210029285127476	19.46777550798085
42-43	19.775736400801144	26.240637333956666	33.78223960689415	20.201386658348035
44-45	19.736864162972882	26.980664438457218	33.01658397634646	20.265887422223443
46-47	20.394514494412853	26.380866158958916	33.53279658127869	19.691822765349542
48-49	19.86591495964867	26.528553798566357	33.44442969396182	20.161101547823147
50-51	20.484519504049974	27.16866670119308	32.68857271223295	19.658241082524
52-53	20.480671216635333	26.772150012856795	32.84439852352604	19.902780246981834
54-55	20.37213359625533	27.78004937013015	31.248964449039597	20.59885258457492
56-57	20.724303815654114	28.2185131066109	30.564964795421222	20.49221828231377
58-59	22.074450307254505	28.219330148609696	29.43154764031821	20.27467190381759
60-61	21.30948239164595	28.8214201251846	29.411669180519134	20.457428302650317
62-63	20.614894837146515	29.00760085245248	29.255377963624042	21.122126346776966
64-65	21.11824894037473	28.96189595263692	29.374489355819623	20.545365751168724
66-67	21.123789556261894	28.17282510058872	30.089250223291096	20.614135119858286
68-69	21.720216845723122	28.348423710766706	29.539512356719012	20.391847086791152
70-71	20.3899399042289	29.430054443630404	29.5719570164054	20.608048635735297
72-73	20.323409566481608	28.281240533126002	30.174938504237453	21.220411396154937
74-75	20.426666050107357	28.780058580140977	29.494638561370945	21.29863680838072
76-77	21.112730915124693	28.268609688812486	29.981682488087625	20.636976907975193
78-79	20.694676513457505	28.455188088088917	29.614054240724546	21.236081157729032
80-81	21.099414816257728	29.063332400266457	29.167843947639028	20.66940883583679
82-83	21.23341586036586	28.50805747311741	29.45870877387978	20.79981789263695
84-85	20.709122403880283	29.207419748178786	28.823101153328867	21.260356694612064
86-87	20.76547003624677	29.417886715437092	29.120545477666038	20.6960977706501
88-89	21.93636635782785	28.756533383119997	28.531205986842934	20.775894272209218
90-91	21.321278242362105	29.59546482744241	28.394973955673148	20.688282974522338
92-93	20.869728203236797	29.600892283305836	28.192254557589948	21.337124955867424
94-95	21.348401928866263	29.751940828539798	28.09399526374833	20.80566197884561
96-97	21.296161619898303	29.03900511851274	28.70535173800748	20.95948152358148
98-99	21.9969773150442	29.236276780664483	27.903997987188824	20.86274791710249
100	21.019393436985833	30.274521763914098	27.900429451927533	20.805655347172536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6837.0
1	4513.5
2	1827.0
3	1361.5
4	1202.5
5	1124.5
6	1133.0
7	1184.0
8	1166.0
9	1208.5
10	1393.5
11	2461.5
12	18265.0
13	22949.5
14	13382.0
15	15650.0
16	19350.0
17	23461.5
18	28273.5
19	33715.0
20	39832.5
21	47466.0
22	56832.5
23	68548.5
24	81584.0
25	95746.0
26	114938.5
27	135157.5
28	151549.0
29	166661.0
30	181201.0
31	195783.5
32	211553.0
33	228755.0
34	246373.5
35	261956.0
36	277427.0
37	291126.5
38	300733.5
39	308421.5
40	315821.5
41	324113.0
42	332739.0
43	334128.0
44	331503.0
45	331665.0
46	329365.5
47	330223.5
48	329371.5
49	341686.0
50	414469.0
51	397150.5
52	283722.5
53	229025.0
54	203467.5
55	180969.0
56	158144.5
57	139241.0
58	129896.0
59	110045.5
60	81186.0
61	62804.0
62	47517.0
63	34856.5
64	24908.0
65	17687.5
66	12286.0
67	8556.0
68	6013.0
69	4206.5
70	2907.5
71	2077.0
72	1551.5
73	1013.5
74	694.5
75	498.5
76	333.0
77	225.5
78	151.5
79	111.0
80	81.0
81	58.5
82	51.5
83	44.5
84	39.5
85	29.0
86	17.0
87	9.0
88	7.0
89	12.5
90	11.5
91	6.5
92	5.0
93	3.0
94	1.5
95	2.0
96	1.5
97	1.5
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025495512537570673
2	0.011623263341530571
3	0.005632973915967799
4	0.0
5	0.0038253778086049985
6	0.0010404186897030078
7	2.837505517371839E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	9.82617651386174E-4
26-27	0.0038831788469218323
28-29	6.147928620972319E-4
30-31	5.254639846984888E-4
32-33	0.005133783130504235
34-35	0.008360131996552956
36-37	0.005270403766525842
38-39	0.005738066712907498
40-41	0.008864577421863506
42-43	0.010677428169073291
44-45	0.009295457889316266
46-47	0.012605880992916745
48-49	0.016678226874330033
50-51	0.06066481703344053
52-53	0.010251802341467515
54-55	0.005648737835508754
56-57	0.003925215965697711
58-59	0.008533535111503458
60-61	0.015238455556256173
62-63	0.012653172751539609
64-65	0.011239674632700675
66-67	0.009605481640288375
68-69	0.006484225571179351
70-71	0.004424406751161275
72-73	0.0032946591840595247
74-75	0.0024171343296130483
76-77	0.0019809992223133025
78-79	0.001586901233789436
80-81	0.0019284528238434537
82-83	0.0014082434789919498
84-85	0.0012348403640414485
86-87	7.356495785778843E-4
88-89	0.0014134981188389348
90-91	8.827794942934611E-4
92-93	3.8358870882989677E-4
94-95	7.303949387308993E-4
96-97	0.0014292620383798895
98-99	0.0036729932530424367
100	1.6814847510351641E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	9515400.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.32921369606741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.11941050419526	62.854882626252305
2	8.977683569891086	12.807422197048693
3	1.702389928376814	3.6429040498566794
4	0.43201875802753836	1.2326223324822367
5	0.1737103648818589	0.6195311868939979
6	0.10082966962684506	0.4315260631030266
7	0.065706987831932	0.32807794434721443
8	0.05203453189678433	0.2969265796192453
9	0.03428248804313648	0.22008086240955818
>10	0.27589458443272785	4.0420993142645
>50	0.04807987714025696	2.2433434688232468
>100	0.015719017746797407	1.8042459669808855
>500	9.139062680889807E-4	0.4444433287543727
>1k	0.0011490367894866384	2.1904723526865806
>5k	8.838742571614188E-5	0.40518649275474145
>10k+	8.838742571614188E-5	6.436235233722763
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	450324	4.732580868907245	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	82110	0.8629169556718582	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	32460	0.3411312188662589	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	17446	0.1833448935409967	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	16597	0.17442251508081635	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	13590	0.14282111104104925	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9565	0.1005212602728209	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.1527839081909323E-4	0.0	0.0	2.1018559387939552E-5	0.0
2	3.3629695020703283E-4	1.0509279693969776E-5	0.0	3.1527839081909326E-5	0.0
3	3.5731550959497237E-4	1.0509279693969776E-5	0.0	6.305567816381865E-5	0.0
4	3.6782478928894214E-4	2.1018559387939552E-5	0.0	9.458351724572798E-5	0.0
5	3.6782478928894214E-4	3.1527839081909326E-5	0.0	9.458351724572798E-5	0.0
6	3.6782478928894214E-4	5.2546398469848874E-5	0.0	1.0509279693969775E-4	0.0
7	3.6782478928894214E-4	7.356495785778842E-5	0.0	1.261113563276373E-4	0.0
8	3.6782478928894214E-4	7.356495785778842E-5	0.0	1.4712991571557685E-4	0.0
9	3.6782478928894214E-4	7.356495785778842E-5	0.0	1.8916703449145596E-4	0.0
10-11	3.6782478928894214E-4	8.407423755175821E-5	0.0	2.4171343296130484E-4	0.0
12-13	3.6782478928894214E-4	8.407423755175821E-5	0.0	2.78495911890199E-4	0.0
14-15	3.9409798852386654E-4	8.407423755175821E-5	0.0	3.310423103600479E-4	0.0
16-17	4.0460726821783637E-4	1.2085671648065242E-4	0.0	3.73079429135927E-4	0.0
18-19	4.098619080648212E-4	1.313659961746222E-4	0.0	4.256258276057759E-4	0.0
20-21	4.20371187758791E-4	1.5763919540954662E-4	0.0	5.044454253105492E-4	0.0
22-23	4.518990268407003E-4	1.6814847510351641E-4	0.0	5.202093448515039E-4	0.0
24-25	5.044454253105492E-4	1.734031149505013E-4	0.0	5.517371839334133E-4	0.0
26-27	5.569918237803981E-4	1.8391239464447107E-4	0.0	5.832650230153225E-4	0.0
28-29	6.358114214851714E-4	1.9967631418542573E-4	0.0	6.305567816381865E-4	0.0
30-31	6.883578199550202E-4	1.9967631418542573E-4	0.0	6.463207011791411E-4	0.0
32-33	8.249784559766274E-4	1.9967631418542573E-4	0.0	6.883578199550202E-4	0.0
34-35	9.405805326102948E-4	2.1544023372638038E-4	0.0	7.46158858271854E-4	0.0
36-37	0.0011560207663366753	2.2069487357336527E-4	0.0	7.881959770477331E-4	0.0
38-39	0.0014134981188389348	2.2594951342035015E-4	0.0	8.039598965886878E-4	0.0
40-41	0.0018391239464447106	2.3120415326733507E-4	0.0	8.617609349055215E-4	0.0
42-43	0.0023015322529793807	2.4171343296130484E-4	0.0	8.932887739874309E-4	0.0
44-45	0.0029163251150766127	2.627319923492444E-4	0.0	9.40580532610295E-4	0.0
46-47	0.003699266452277361	2.8900519158416883E-4	0.0	9.615990919982344E-4	0.0
48-49	0.00466612018412258	3.1527839081909323E-4	0.0	9.668537318452193E-4	0.0
50-51	0.006242512138218047	3.2578767051306306E-4	0.0	9.983815709271285E-4	0.0
52-53	0.008092145364356727	3.3629695020703283E-4	0.0	0.0010614372490909472	0.0
54-55	0.010635391050297413	3.5206086974798746E-4	0.0	0.001124492927254766	0.0
56-57	0.013830212077264224	3.73079429135927E-4	0.0	0.0011350022069487357	0.0
58-59	0.018154780671332787	3.9409798852386654E-4	0.0	0.0011455114866427054	0.0
60-61	0.023892847384240284	4.0460726821783637E-4	0.0	0.0011717846858776298	0.0
62-63	0.03158564012022616	4.571536666876852E-4	0.0	0.0011822939655715997	0.0
64-65	0.042058137335267046	5.044454253105492E-4	0.0	0.0012506042835824032	0.0
66-67	0.05575698341635664	5.044454253105492E-4	0.0	0.001266368203123358	0.0
68-69	0.07520966012989469	5.097000651575342E-4	0.0	0.0012873867625112975	0.0
70-71	0.10002732412720433	5.412279042394434E-4	0.0	0.0013294238812871766	0.0
72-73	0.1313554868949282	5.885196628623074E-4	0.0	0.0013662063602160707	0.0
74-75	0.17390756037581184	6.410660613321563E-4	0.0	0.0014029888391449649	0.0
76-77	0.22751014145490467	6.515753410261261E-4	0.0	0.0014555352376148139	0.0
78-79	0.2969817348718919	6.831031801080354E-4	0.0	0.001487063076696723	0.0
80-81	0.3834783613931101	6.936124598020052E-4	0.0	0.001508081636084663	0.0
82-83	0.49375223322193496	7.251402988839145E-4	0.0	0.0015606280345545117	0.0
84-85	0.6276772390020389	7.303949387308993E-4	0.0	0.0016079197931773755	0.0
86-87	0.790665657775816	7.46158858271854E-4	0.0	0.0016604661916472245	0.0
88	0.9320049603800156	7.566681379658238E-4	0.0	0.0016709754713411943	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	9205	0.0	86.92444	1
ATCGAGT	4325	0.0	73.37089	1
TCGAGTG	5050	0.0	69.43059	2
GTGGAAT	193485	0.0	62.58826	6
TGGAATC	194100	0.0	62.535213	7
GAATCAC	195795	0.0	62.250656	9
GGAATCA	195540	0.0	62.08911	8
AGTGGAA	195885	0.0	61.836346	5
ACGAGTG	188410	0.0	60.351654	2
CGAGTGG	194395	0.0	60.25011	3
AACGAGT	175825	0.0	59.62527	1
GAGTGGA	203805	0.0	59.46101	4
GACGAGT	2870	0.0	50.615402	1
ATGAGTG	9630	0.0	42.071148	2
TGAGTGG	10205	0.0	41.95323	3
GAGTACT	77355	0.0	40.891815	20-21
AGTACTT	79040	0.0	40.730576	20-21
GTACTTT	78055	0.0	39.739502	22-23
CATGGGG	36485	0.0	38.119774	24-25
AATGAGT	10185	0.0	37.018555	1
>>END_MODULE