FastQCFastQC Report
Thu 26 May 2016
SRR2132626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132626_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7432165
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT3867525.20375960436831No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG854191.1493151726313935No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT271640.36549242380921304No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT142120.19122288054692005No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA139600.18783221308999465No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118680.1596842911856774No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA87300.11746240832920152No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86930.11696457223433548No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT82800.086.36871
ATCGAGT35550.080.941151
TCGAGTG45250.069.598472
GTGGAAT1803700.059.4720086
GAATCAC1822800.059.2395259
TGGAATC1816950.059.1716167
AGTGGAA1820250.058.9727865
GGAATCA1829200.058.849858
CGAGTGG1825250.056.6213043
ACGAGTG1774950.056.5055052
GAGTGGA1903050.056.4168054
AACGAGT1662500.055.668461
GACGAGT21750.049.287081
ATGAGTG81500.048.5044862
TGAGTGG87300.048.399653
AATGAGT82300.044.1036951
GAGTACT651550.041.01959220-21
AGTACTT666050.040.95212620-21
GTACTTT661750.039.8369522-23
CATGGGG335450.038.7787824-25