##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2132626_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7432165
Sequences flagged as poor quality	0
Sequence length	100
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	26.601141255609907	31.0	22.0	33.0	16.0	34.0
2	27.896806650552026	31.0	25.0	34.0	16.0	34.0
3	28.095241157859117	31.0	25.0	34.0	16.0	34.0
4	33.89551308400715	35.0	33.0	37.0	28.0	37.0
5	34.95982866365319	35.0	35.0	37.0	32.0	37.0
6	35.35730719110784	37.0	35.0	37.0	33.0	37.0
7	35.63203481085256	37.0	35.0	37.0	33.0	37.0
8	35.73710527147877	37.0	35.0	37.0	35.0	37.0
9	37.510272444166674	39.0	37.0	39.0	35.0	39.0
10-11	37.703286390977595	39.0	37.0	39.0	35.0	39.0
12-13	37.71372735938989	39.0	37.0	39.0	35.0	39.0
14-15	39.14030084100663	40.5	39.0	41.0	36.0	41.0
16-17	39.10213174761324	40.5	39.0	41.0	36.0	41.0
18-19	39.17799221357437	40.5	39.0	41.0	36.0	41.0
20-21	39.16268718468979	40.5	39.0	41.0	36.0	41.0
22-23	39.10417099189805	40.0	39.0	41.0	36.0	41.0
24-25	38.97370799760232	40.0	39.0	41.0	35.0	41.0
26-27	38.994562418891405	40.0	39.0	41.0	35.0	41.0
28-29	38.94168717998053	40.0	39.0	41.0	35.0	41.0
30-31	38.845526572674316	40.0	39.0	41.0	35.0	41.0
32-33	38.75468070205653	40.0	38.5	41.0	35.0	41.0
34-35	38.67924454045355	40.0	38.0	41.0	35.0	41.0
36-37	38.53804026148505	40.0	38.0	41.0	34.5	41.0
38-39	38.20512643354931	40.0	37.0	41.0	34.0	41.0
40-41	38.04572900359451	40.0	37.0	41.0	33.0	41.0
42-43	37.93368406110467	40.0	37.0	41.0	33.0	41.0
44-45	37.80630839062373	40.0	37.0	41.0	33.0	41.0
46-47	37.69801275940456	40.0	36.5	41.0	33.0	41.0
48-49	37.56430669932651	40.0	36.0	41.0	33.0	41.0
50-51	37.348060020196	40.0	36.0	41.0	33.0	41.0
52-53	36.88775874862843	40.0	35.5	41.0	31.5	41.0
54-55	36.16617513201066	40.0	35.0	41.0	29.0	41.0
56-57	35.60785989008586	40.0	35.0	41.0	27.0	41.0
58-59	35.36887905475726	39.0	35.0	41.0	26.0	41.0
60-61	35.092423001911285	39.0	35.0	41.0	26.0	41.0
62-63	34.841866064598946	39.0	35.0	41.0	25.0	41.0
64-65	34.569431316446824	38.5	35.0	40.5	24.5	41.0
66-67	34.21724988613681	37.5	34.5	40.0	23.0	41.0
68-69	33.90130365781707	37.0	34.0	40.0	22.5	41.0
70-71	33.6044822067325	36.5	34.0	39.5	22.0	41.0
72-73	33.27860186096514	36.0	34.0	39.0	22.0	41.0
74-75	32.9809157224039	36.0	34.0	39.0	22.0	40.5
76-77	32.725539462592664	35.0	34.0	37.5	21.5	40.0
78-79	32.39533850499821	35.0	34.0	37.0	20.5	39.0
80-81	32.06757714609404	35.0	33.0	37.0	20.0	39.0
82-83	31.7144509708813	35.0	33.0	36.0	18.5	39.0
84-85	31.389887737960606	35.0	33.0	36.0	17.5	37.0
86-87	31.23298345771387	35.0	33.0	36.0	15.5	37.0
88-89	31.08515984238778	35.0	33.0	35.0	11.5	36.5
90-91	30.890081288561277	35.0	33.0	35.0	6.5	36.0
92-93	30.737714246118056	35.0	33.0	35.0	2.0	36.0
94-95	30.561520229973368	35.0	33.0	35.0	2.0	36.0
96-97	30.37435942285996	35.0	33.0	35.0	2.0	36.0
98-99	30.233493403335366	35.0	33.0	35.0	2.0	35.5
100	30.15469597351512	35.0	33.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	3.9406012484773925
1101	2	4.077800391467228
1101	3	3.4905050765281658
1101	4	1.9642790386202833
1101	5	1.099946741336872
1101	6	0.8468154976365057
1101	7	0.4865751140209724
1101	8	0.5344643640020337
1101	9	0.6229654165878031
1101	10-11	0.4223230724705118
1101	12-13	0.41905571874281833
1101	14-15	0.5338455390135195
1101	16-17	0.5335011087409853
1101	18-19	0.4501797779765937
1101	20-21	0.48947281782560736
1101	22-23	0.4937794714953654
1101	24-25	0.54274952238422
1101	26-27	0.469011724241426
1101	28-29	0.44042594272588786
1101	30-31	0.4778758999165831
1101	32-33	0.4484241748341802
1101	34-35	0.47935752984168545
1101	36-37	0.4274489716087757
1101	38-39	0.48086200583760075
1101	40-41	0.48674237756331706
1101	42-43	0.5342388145180479
1101	44-45	0.48902102435248906
1101	46-47	0.48932727402634413
1101	48-49	0.4736272440500784
1101	50-51	0.4938849347948562
1101	52-53	0.488236013548196
1101	54-55	0.4701598306245387
1101	56-57	0.5033260775781727
1101	58-59	0.448054899828513
1101	60-61	0.40985175169530663
1101	62-63	0.4319531805350465
1101	64-65	0.42817649122458334
1101	66-67	0.4016911249866695
1101	68-69	0.4634345646493685
1101	70-71	0.45724380291283495
1101	72-73	0.41415848106132813
1101	74-75	0.47174857086073274
1101	76-77	0.43055348203114363
1101	78-79	0.41813933236278444
1101	80-81	0.40839901273970014
1101	82-83	0.35693710931977307
1101	84-85	0.40589049488693973
1101	86-87	0.4088676789572325
1101	88-89	0.4145708890019364
1101	90-91	0.41240780315091286
1101	92-93	0.42298480929344606
1101	94-95	0.4717448981245944
1101	96-97	0.48660613640674555
1101	98-99	0.5061488395916385
1101	100	0.46985755976671584
1102	1	2.974889257946913
1102	2	3.504485537897253
1102	3	4.200272370907111
1102	4	2.1322525085196915
1102	5	1.0899055495234578
1102	6	0.8318360666447617
1102	7	0.4256661821291985
1102	8	0.49113697179816995
1102	9	0.6011473708441386
1102	10-11	0.39522846041223403
1102	12-13	0.3164104380325554
1102	14-15	0.45768166478845984
1102	16-17	0.5316705900272964
1102	18-19	0.4235363874363216
1102	20-21	0.4254934672918651
1102	22-23	0.35512740984379576
1102	24-25	0.41401531523623447
1102	26-27	0.37436750649467143
1102	28-29	0.38521987804286084
1102	30-31	0.40508050290612374
1102	32-33	0.41975877234197156
1102	34-35	0.37255936561350467
1102	36-37	0.3722656123830532
1102	38-39	0.3837718881940617
1102	40-41	0.3918719359310998
1102	42-43	0.37735846365465164
1102	44-45	0.3259953663155102
1102	46-47	0.36567022864557686
1102	48-49	0.409446629811292
1102	50-51	0.48979874361316433
1102	52-53	0.4812425349194527
1102	54-55	0.3962511541889384
1102	56-57	0.4235167512047937
1102	58-59	0.31373249989806595
1102	60-61	0.28187378659003315
1102	62-63	0.278379677787882
1102	64-65	0.30977769747771333
1102	66-67	0.26819660400339274
1102	68-69	0.23448645943559399
1102	70-71	0.2688785380853176
1102	72-73	0.24914783599928825
1102	74-75	0.25668774040902775
1102	76-77	0.22951726666853745
1102	78-79	0.2535653124533397
1102	80-81	0.2773239283240514
1102	82-83	0.2511859673025185
1102	84-85	0.17517868196534891
1102	86-87	0.2249257457513103
1102	88-89	0.24529735366808936
1102	90-91	0.24947625013640007
1102	92-93	0.2599990638111791
1102	94-95	0.27495855893021925
1102	96-97	0.2503606000662657
1102	98-99	0.22633837398973355
1102	100	0.2469812656644308
1103	1	2.595709325338909
1103	2	3.2347504714464748
1103	3	3.114001740771119
1103	4	1.7107995244722503
1103	5	0.92599228290414
1103	6	0.7619260074959584
1103	7	0.3981791877746801
1103	8	0.4992765250247544
1103	9	0.5563481828313215
1103	10-11	0.3695783458654276
1103	12-13	0.3462926354756206
1103	14-15	0.40800483822548017
1103	16-17	0.48167523720705674
1103	18-19	0.4121545089547567
1103	20-21	0.4260935454811161
1103	22-23	0.39793028860835733
1103	24-25	0.426892684686635
1103	26-27	0.3329988416015581
1103	28-29	0.3734860269146978
1103	30-31	0.4094958473876886
1103	32-33	0.3876037101534493
1103	34-35	0.4155111112422034
1103	36-37	0.44070311037713594
1103	38-39	0.4654211664537442
1103	40-41	0.4389128918982621
1103	42-43	0.44026014543125314
1103	44-45	0.42795286614567374
1103	46-47	0.4459531447599687
1103	48-49	0.48945640159219295
1103	50-51	0.5364787213200088
1103	52-53	0.5169591209450033
1103	54-55	0.4376525931465949
1103	56-57	0.4558967166533847
1103	58-59	0.41282536177403983
1103	60-61	0.4911701376151356
1103	62-63	0.4535054536568879
1103	64-65	0.5357819530955226
1103	66-67	0.566180062025019
1103	68-69	0.5164569348512558
1103	70-71	0.4907966335961049
1103	72-73	0.4605204852306741
1103	74-75	0.49260521283749625
1103	76-77	0.5187662029427855
1103	78-79	0.4667443604093009
1103	80-81	0.5104548197072063
1103	82-83	0.5289962101113446
1103	84-85	0.5231559323059756
1103	86-87	0.484595005971034
1103	88-89	0.4944430747811275
1103	90-91	0.5389719260723957
1103	92-93	0.5424169218391555
1103	94-95	0.5670689166152947
1103	96-97	0.5395961995396839
1103	98-99	0.567219451955399
1103	100	0.5937990777393622
1104	1	2.583178825211874
1104	2	3.3290160973341614
1104	3	1.7126849566675055
1104	4	1.1198426973618112
1104	5	0.6021820600441856
1104	6	0.5626161168470389
1104	7	0.3075643739066578
1104	8	0.3720631372167915
1104	9	0.46882852045238366
1104	10-11	0.3396715590257955
1104	12-13	0.3251548091281222
1104	14-15	0.41155032406292236
1104	16-17	0.5011253888016469
1104	18-19	0.38428201172266085
1104	20-21	0.4009983326278004
1104	22-23	0.3589693434989343
1104	24-25	0.4042377356837008
1104	26-27	0.3806821532368545
1104	28-29	0.3914280194843798
1104	30-31	0.3284146966948711
1104	32-33	0.27925183049070057
1104	34-35	0.36724016386129676
1104	36-37	0.35500110244074534
1104	38-39	0.3461561511781568
1104	40-41	0.3344080305169328
1104	42-43	0.3368135914377035
1104	44-45	0.2573080704838233
1104	46-47	0.31725291473713924
1104	48-49	0.30523918945689843
1104	50-51	0.3260740018445105
1104	52-53	0.343358074832949
1104	54-55	0.25213649316248166
1104	56-57	0.24258152677937517
1104	58-59	0.26686488600088154
1104	60-61	0.22287513162437733
1104	62-63	0.2429584823513693
1104	64-65	0.2405834979631436
1104	66-67	0.22476994792377525
1104	68-69	0.2734779063501023
1104	70-71	0.29877628158841674
1104	72-73	0.2157331993006011
1104	74-75	0.25001723328872316
1104	76-77	0.2490502113481341
1104	78-79	0.23521057738077644
1104	80-81	0.2420020235174576
1104	82-83	0.22629822990340642
1104	84-85	0.2243936694498494
1104	86-87	0.27535552323058354
1104	88-89	0.2734278091686697
1104	90-91	0.24236968324519026
1104	92-93	0.26039114894905424
1104	94-95	0.25750946749916537
1104	96-97	0.29094929903134314
1104	98-99	0.34005276099814097
1104	100	0.29666893203935274
1105	1	1.4446779893662018
1105	2	2.679494541198686
1105	3	1.4374932033622763
1105	4	0.9500210417121195
1105	5	0.6095732008720702
1105	6	0.5648392163941836
1105	7	0.29227668496698556
1105	8	0.3490882418290937
1105	9	0.475629772961355
1105	10-11	0.29194748427746475
1105	12-13	0.3107495134898457
1105	14-15	0.36436686695935094
1105	16-17	0.44438201908396735
1105	18-19	0.3378040725434772
1105	20-21	0.37599023603213055
1105	22-23	0.31441541030292086
1105	24-25	0.3462449571335142
1105	26-27	0.32863108015107656
1105	28-29	0.27946391174941
1105	30-31	0.2794612872602471
1105	32-33	0.2723736738154443
1105	34-35	0.28586235773362034
1105	36-37	0.307127961035782
1105	38-39	0.3107687397116905
1105	40-41	0.25301584165600133
1105	42-43	0.33448370079707956
1105	44-45	0.3592315935379773
1105	46-47	0.38178195164619666
1105	48-49	0.36920281743098826
1105	50-51	0.3764324403041499
1105	52-53	0.23005467669634072
1105	54-55	0.31737670085868075
1105	56-57	0.3366415602799293
1105	58-59	0.34130064724005393
1105	60-61	0.26576264784227277
1105	62-63	0.012276290949351676
1105	64-65	0.14699541016337037
1105	66-67	0.27501320886678116
1105	68-69	0.3494035000692648
1105	70-71	0.310113232763257
1105	72-73	0.2442772964733777
1105	74-75	0.28130887759218126
1105	76-77	0.22884632264676696
1105	78-79	0.2526072151883767
1105	80-81	0.2574393818988767
1105	82-83	0.26244119396351806
1105	84-85	0.2796320459409358
1105	86-87	0.3169949903870446
1105	88-89	0.29035550819195066
1105	90-91	0.2583872888882226
1105	92-93	0.28077416646247144
1105	94-95	0.2895202344469219
1105	96-97	0.27183825974667286
1105	98-99	0.29708174502331275
1105	100	0.30694172216357885
1106	1	1.9929538198130103
1106	2	2.9329941149499916
1106	3	1.2581329682928626
1106	4	0.8919201218438033
1106	5	0.5279054924018922
1106	6	0.5056917904114187
1106	7	0.25916715321002926
1106	8	0.34594234222206666
1106	9	0.36306001328256343
1106	10-11	0.2433530167682889
1106	12-13	0.2170397617610078
1106	14-15	0.18842080869691102
1106	16-17	0.28863727377287773
1106	18-19	0.09701800101365876
1106	20-21	0.21904377762361804
1106	22-23	0.2416700960028706
1106	24-25	0.27123039925943715
1106	26-27	0.20771920926232212
1106	28-29	0.23516483059785287
1106	30-31	0.23739467289645688
1106	32-33	0.15341126489600043
1106	34-35	0.15856122897118752
1106	36-37	0.19335593887924318
1106	38-39	0.20804368867074174
1106	40-41	0.1562233091882561
1106	42-43	0.17037033667573098
1106	44-45	0.1141078157577553
1106	46-47	0.11962000613328172
1106	48-49	0.022732274654231333
1106	50-51	0.15227441705679468
1106	52-53	0.1432296745840418
1106	54-55	0.09870101423118172
1106	56-57	0.18451786516031632
1106	58-59	0.1452143593698878
1106	60-61	-0.009048043448053988
1106	62-63	0.11167588658290128
1106	64-65	-0.0868715790017518
1106	66-67	-0.2223414932103367
1106	68-69	0.0863890818223112
1106	70-71	0.1942968431875869
1106	72-73	0.17154021583473877
1106	74-75	0.2228749592965258
1106	76-77	0.1894372827429649
1106	78-79	0.12183826756130856
1106	80-81	0.1348167829620337
1106	82-83	0.19899430000910812
1106	84-85	0.12195160838515307
1106	86-87	0.1701204269859211
1106	88-89	0.18153715327158793
1106	90-91	0.1866137861094721
1106	92-93	0.2011783789473398
1106	94-95	0.19008111512554748
1106	96-97	0.19283479225532218
1106	98-99	0.22761981275468912
1106	100	0.21280351013393783
1107	1	1.6510524711553494
1107	2	0.9651214967128432
1107	3	0.5654571657695442
1107	4	0.4426321870466623
1107	5	0.28298941478158923
1107	6	0.3982259146201059
1107	7	0.21042212758259637
1107	8	0.2826589270783586
1107	9	0.3680300445450939
1107	10-11	0.2384671680770154
1107	12-13	0.22771079636000735
1107	14-15	0.29375446768997193
1107	16-17	0.34904732735686395
1107	18-19	0.26250949541469737
1107	20-21	0.2833116152019599
1107	22-23	0.24298134109798042
1107	24-25	0.22414385123744296
1107	26-27	0.2338818356909158
1107	28-29	0.3015761250777871
1107	30-31	0.27466688421464625
1107	32-33	0.20265510324185954
1107	34-35	0.20443150324918946
1107	36-37	0.19891089967484987
1107	38-39	0.2150662029200987
1107	40-41	0.25354520305268125
1107	42-43	0.23565915388903136
1107	44-45	0.260375588517455
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2216	74-75	-1.244038305144059
2216	76-77	-1.161357847764819
2216	78-79	-1.128172947001346
2216	80-81	-1.061893574459745
2216	82-83	-1.1167364511023656
2216	84-85	-1.0993774463998633
2216	86-87	-1.0397316263599663
2216	88-89	-1.0566467886757138
2216	90-91	-1.1034060337909573
2216	92-93	-1.1174705154649267
2216	94-95	-1.1220210794157417
2216	96-97	-1.1768484316296828
2216	98-99	-1.262858910953014
2216	100	-1.3385254530729895
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	4167.0
3	79.0
4	203.0
5	340.0
6	607.0
7	1026.0
8	1706.0
9	3000.0
10	5243.0
11	9557.0
12	9726.0
13	13327.0
14	14336.0
15	16087.0
16	19144.0
17	24566.0
18	36126.0
19	62917.0
20	120693.0
21	146495.0
22	65935.0
23	40753.0
24	39393.0
25	43047.0
26	47804.0
27	56832.0
28	71357.0
29	89004.0
30	109954.0
31	137579.0
32	174055.0
33	227745.0
34	315730.0
35	477641.0
36	806042.0
37	1480985.0
38	2015757.0
39	738941.0
40	4266.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	17.614412146574704	39.125272520425824	27.01099852668382	16.24931680631565
2	19.482953742640873	37.17258873002523	25.229268292682928	18.115189234650966
3	20.88234241405767	23.899705938787797	21.718655515645903	33.49929613150863
4	32.164772983376984	25.336466561224086	23.299227075825147	19.199533379573786
5	17.838994214386073	36.892647111458444	24.26716409114138	21.0011945830141
6	34.46550864399724	22.558716291801474	24.957555703681898	18.018219360519392
7	19.54932752863308	22.57078649508392	38.526504755428384	19.35338122085462
8	33.88645165977881	22.838863238369978	24.443900263247652	18.830784838603556
9	33.633793114119506	24.61729522958653	24.090342450685633	17.658569205608327
10-11	17.770595781982774	39.689706835087755	24.3712363759416	18.16846100698787
12-13	17.617470817722698	24.12558790069919	32.05875407771491	26.19818720386321
14-15	17.78059959648366	31.69845529532781	24.406609648736268	26.11433545945226
16-17	25.77800008476669	23.85469106242932	31.97340209750456	18.393906755299433
18-19	18.185118333621496	23.976257254783768	32.01756823213693	25.821056179457802
20-21	25.94979390258424	31.738497463390548	24.318089009057253	17.99361962496796
22-23	25.562632153618765	24.113054540635197	32.12448189726681	18.199831408479223
24-25	18.00563179932194	31.60642177973524	24.310627316729374	26.077319104213448
26-27	18.23135164810028	27.57756102418093	35.92546445116922	18.26562287654957
28-29	25.823693952408334	23.88732981086501	32.088317176614574	18.20065906011209
30-31	23.66219116892581	25.30686157621463	32.41717647118976	18.613770783669803
32-33	20.04949544157936	26.537557444900088	33.08179026185529	20.331156851665252
34-35	20.40425205961366	26.295007173132017	33.42341097952426	19.87732978773006
36-37	20.43541110805068	25.655149149308894	34.26021299605034	19.649226746590085
38-39	21.375605694425776	25.674002614993302	33.39495400752366	19.555437683057267
40-41	19.790504809843355	27.395303874800497	33.23886423941446	19.575327075941686
42-43	19.892628157038587	25.657553633104847	33.94602422621613	20.503793983640435
44-45	19.836691985316246	26.57091721384917	33.02762700585086	20.564763794983733
46-47	20.656696903671413	25.771606111580486	33.73789139936537	19.83380558538273
48-49	19.911566709442933	26.00842938255762	33.59498807145072	20.48501583654873
50-51	20.787185565423837	26.748956815746457	32.75067421752598	19.71318340130372
52-53	20.776888700258855	26.22236533942876	32.967321394245374	20.03342456606701
54-55	20.54149066490988	27.509949212459418	31.000226864649804	20.9483332579809
56-57	20.928445157649506	27.955791990867436	30.53159193128617	20.584170920196893
58-59	22.647460608454985	27.82569140410122	29.140942985737556	20.385905001706238
60-61	21.633218976524166	28.65674608394598	29.093054531221753	20.61698040830811
62-63	20.66792958984602	28.74165550591108	29.071394857271848	21.519020046971054
64-65	21.43054763109451	28.65596505581811	29.17492006760612	20.738567245481264
66-67	21.438448888950788	27.70883740075839	30.030176595419867	20.822537114870958
68-69	22.217719725093062	27.777755725035735	29.491419737815622	20.51310481205558
70-71	20.402942071863116	29.34309987267617	29.510952035751586	20.743006019709124
72-73	20.348842489792176	27.81444879362625	30.254907832382244	21.58180088419933
74-75	20.535113960775227	28.42974563868317	29.34287963636104	21.692260764180563
76-77	21.411633619686597	27.74216648083788	29.988022772542138	20.858177126933384
78-79	20.80643944895796	28.04895597986266	29.608805198071142	21.53579937310824
80-81	21.436973768332564	28.762248422486543	28.98345989927245	20.817317909908446
82-83	21.506285293407092	28.13735712849372	29.428343093846713	20.928014484252472
84-85	20.789047332452558	29.018523594106583	28.566335790650516	21.626093282790343
86-87	20.8918100830039	29.15259514427948	29.079083149957004	20.876511622759615
88-89	22.45696510646852	28.42796555122964	28.227483303966274	20.88758603833557
90-91	21.67951363436249	29.37171627307691	28.16892766839646	20.77984242416414
92-93	21.025320242705444	29.33887853109264	27.933609669305724	21.70219155689619
94-95	21.59514049598119	29.552591109140984	27.823621979014995	21.02864641586283
96-97	21.61683848057446	28.673349773791212	28.579883241174198	21.12992850446013
98-99	22.46244884127459	28.91474038012784	27.6483539964137	20.97445678218387
100	21.122990119424266	30.26476293964687	27.64385525711375	20.968391683815117
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9316.0
1	5798.5
2	1852.0
3	1309.5
4	1158.5
5	1066.5
6	1027.0
7	1081.0
8	1104.0
9	1169.0
10	1303.0
11	2392.0
12	19796.0
13	24044.0
14	11975.0
15	13234.5
16	15848.0
17	19137.5
18	23317.0
19	27831.5
20	32277.0
21	37431.5
22	44182.5
23	52708.5
24	62190.0
25	72573.0
26	86375.0
27	101676.5
28	113354.5
29	123181.5
30	133967.0
31	144056.5
32	155017.5
33	168249.0
34	182810.0
35	193286.5
36	202419.0
37	211764.5
38	217407.0
39	222381.5
40	226927.0
41	231907.5
42	235745.5
43	237252.5
44	237920.0
45	238654.5
46	239557.0
47	243867.0
48	248289.0
49	272552.5
50	360582.0
51	351361.0
52	240548.0
53	191439.0
54	172345.0
55	157447.5
56	142584.0
57	129528.5
58	123124.0
59	106482.0
60	80124.5
61	61428.5
62	47206.5
63	35881.5
64	25572.0
65	18043.5
66	12845.0
67	8656.0
68	5807.5
69	3827.0
70	2455.0
71	1708.0
72	1226.0
73	842.0
74	587.0
75	395.0
76	283.5
77	196.0
78	141.5
79	104.5
80	66.5
81	50.0
82	39.0
83	31.5
84	25.0
85	16.5
86	15.0
87	15.5
88	14.5
89	11.5
90	5.5
91	3.5
92	4.5
93	4.5
94	3.5
95	2.0
96	0.5
97	0.0
98	0.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.026748598826855968
2	0.012311352075741053
3	0.005099456214979081
4	0.0
5	0.0038077733742455937
6	9.553071009591417E-4
7	3.6328579895629335E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	0.0
18-19	0.0
20-21	0.0
22-23	0.0
24-25	0.001103312426459854
26-27	0.0037001331375178022
28-29	8.880319530042726E-4
30-31	9.149420121862204E-4
32-33	0.00497163343386483
34-35	0.008254660654062444
36-37	0.00516673136293395
38-39	0.005462742013935374
40-41	0.008530488760677407
42-43	0.010683293495233219
44-45	0.009236877814203532
46-47	0.012492994975219197
48-49	0.016859152077490207
50-51	0.06278116807148389
52-53	0.010824571305938445
54-55	0.005664567457799982
56-57	0.004023053847701174
58-59	0.008355573375994747
60-61	0.015924027520917525
62-63	0.013354116869041525
64-65	0.011907701188011837
66-67	0.010003814500889042
68-69	0.006545871896008767
70-71	0.004406522191043929
72-73	0.003276299705402127
74-75	0.0023949952671933413
76-77	0.0019442517758957183
78-79	0.0013522304738928697
80-81	0.0018366115391679275
82-83	0.001258045266756053
84-85	0.001022582248914011
86-87	5.987488167983353E-4
88-89	0.0011504050300282624
90-91	8.207568050494034E-4
92-93	3.834683433427541E-4
94-95	6.525689351622307E-4
96-97	0.001251317751960566
98-99	0.0036395855043584208
100	9.41852071368168E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7432165.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.3196907647512
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.88769363133962	58.49252666524224
2	9.338412377902577	12.573180670282527
3	1.981485516142163	4.0017897660457145
4	0.6214772739807819	1.673506316068272
5	0.2906116613487106	0.9781943587312896
6	0.17521911501788254	0.7077417983446335
7	0.1282934616334032	0.6045673315010122
8	0.08875598703418694	0.4780020480529385
9	0.06851407346784369	0.4151111614999813
>10	0.334531936283965	4.261398403880026
>50	0.06507809063999823	2.8684451153214354
>100	0.01699015164256964	1.8944099440637674
>500	0.0011587643585112886	0.522617331918466
>1k	0.0015582114412979237	2.8038780888343227
>5k	9.988534838712476E-5	0.47084548709138513
>10k+	1.198624180645497E-4	7.253785513122043
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	386752	5.20375960436831	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG	85419	1.1493151726313935	No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	27164	0.36549242380921304	No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	14212	0.19122288054692005	No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA	13960	0.18783221308999465	No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	11868	0.1596842911856774	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	8730	0.11746240832920152	No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	8693	0.11696457223433548	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.6328579895629335E-4	0.0	0.0	0.0	1.3455029590973829E-5
2	3.6328579895629335E-4	2.6910059181947657E-5	0.0	0.0	1.3455029590973829E-5
3	3.90195858138241E-4	4.0365088772921486E-5	0.0	0.0	1.3455029590973829E-5
4	4.171059173201887E-4	4.0365088772921486E-5	0.0	0.0	1.3455029590973829E-5
5	4.305609469111625E-4	5.3820118363895314E-5	0.0	0.0	1.3455029590973829E-5
6	4.4401597650213634E-4	5.3820118363895314E-5	0.0	0.0	1.3455029590973829E-5
7	4.4401597650213634E-4	5.3820118363895314E-5	0.0	0.0	1.3455029590973829E-5
8	4.4401597650213634E-4	5.3820118363895314E-5	0.0	0.0	1.3455029590973829E-5
9	4.5747100609311016E-4	5.3820118363895314E-5	0.0	0.0	1.3455029590973829E-5
10-11	4.641985208885971E-4	6.727514795486914E-5	0.0	0.0	1.3455029590973829E-5
12-13	4.70926035684084E-4	6.727514795486914E-5	0.0	1.3455029590973829E-5	3.363757397743457E-5
14-15	4.7765355047957086E-4	6.727514795486914E-5	0.0	1.3455029590973829E-5	4.0365088772921486E-5
16-17	5.112911244570056E-4	6.727514795486914E-5	0.0	1.3455029590973829E-5	5.3820118363895314E-5
18-19	5.382011836389531E-4	6.727514795486914E-5	0.0	1.3455029590973829E-5	5.3820118363895314E-5
20-21	5.852937872073615E-4	6.727514795486914E-5	0.0	1.3455029590973829E-5	5.3820118363895314E-5
22-23	6.592964499577175E-4	8.073017754584297E-5	0.0	1.3455029590973829E-5	5.3820118363895314E-5
24-25	7.198440831170999E-4	8.073017754584297E-5	0.0	1.3455029590973829E-5	5.3820118363895314E-5
26-27	7.602091718900213E-4	8.073017754584297E-5	0.0	1.3455029590973829E-5	5.3820118363895314E-5
28-29	8.543943790268381E-4	8.073017754584297E-5	0.0	1.3455029590973829E-5	5.3820118363895314E-5
30-31	0.0010023997045275502	8.073017754584297E-5	0.0	1.3455029590973829E-5	5.3820118363895314E-5
32-33	0.0012109526631876445	8.073017754584297E-5	0.0	1.3455029590973829E-5	5.3820118363895314E-5
34-35	0.0014195056218477388	8.073017754584297E-5	0.0	1.3455029590973829E-5	6.727514795486914E-5
36-37	0.0017693363912130583	8.073017754584297E-5	0.0	1.3455029590973829E-5	6.727514795486914E-5
38-39	0.002112439645782891	8.073017754584297E-5	0.0	1.3455029590973829E-5	6.727514795486914E-5
40-41	0.002516090533512106	9.41852071368168E-5	0.0	1.3455029590973829E-5	6.727514795486914E-5
42-43	0.003188842013060797	1.0764023672779063E-4	0.0	1.3455029590973829E-5	6.727514795486914E-5
44-45	0.003935596155359844	1.0764023672779063E-4	0.0	1.3455029590973829E-5	6.727514795486914E-5
46-47	0.004924540830296421	1.3455029590973827E-4	0.0	1.3455029590973829E-5	6.727514795486914E-5
48-49	0.006027853256756275	1.4127781070522518E-4	0.0	1.3455029590973829E-5	6.727514795486914E-5
50-51	0.0074877239673769354	1.6818786988717285E-4	0.0	1.3455029590973829E-5	6.727514795486914E-5
52-53	0.00943870325806814	1.7491538468265977E-4	0.0	1.3455029590973829E-5	8.073017754584297E-5
54-55	0.01175296834771564	1.7491538468265977E-4	0.0	1.3455029590973829E-5	8.073017754584297E-5
56-57	0.01484762515363962	1.7491538468265977E-4	0.0	1.3455029590973829E-5	8.073017754584297E-5
58-59	0.01865539852788521	1.7491538468265977E-4	0.0	1.3455029590973829E-5	8.073017754584297E-5
60-61	0.02365394202093199	1.7491538468265977E-4	0.0	1.3455029590973829E-5	8.073017754584297E-5
62-63	0.030664012437829354	1.883704142736336E-4	0.0	1.3455029590973829E-5	8.073017754584297E-5
64-65	0.03990761776682837	1.883704142736336E-4	0.0	1.3455029590973829E-5	8.073017754584297E-5
66-67	0.05116947753447347	1.883704142736336E-4	0.0	1.3455029590973829E-5	8.073017754584297E-5
68-69	0.06633329588350098	1.883704142736336E-4	0.0	1.3455029590973829E-5	8.073017754584297E-5
70-71	0.08595745654193629	1.950979290691205E-4	0.0	1.3455029590973829E-5	8.073017754584297E-5
72-73	0.11117890951021675	2.018254438646074E-4	0.0	1.3455029590973829E-5	8.073017754584297E-5
74-75	0.14488375863560618	2.018254438646074E-4	0.0	1.3455029590973829E-5	9.41852071368168E-5
76-77	0.18967555214395804	2.1528047345558126E-4	0.0	1.3455029590973829E-5	1.0764023672779063E-4
78-79	0.2456888403311821	2.2873550304655508E-4	0.0	1.3455029590973829E-5	1.2109526631876445E-4
80-81	0.31704758976691183	2.2873550304655508E-4	0.0	1.3455029590973829E-5	1.3455029590973827E-4
82-83	0.4065840841800471	2.2873550304655508E-4	0.0	1.3455029590973829E-5	1.6146035509168594E-4
84-85	0.5146885194287263	2.2873550304655508E-4	0.0	1.3455029590973829E-5	1.6146035509168594E-4
86-87	0.6477722171130484	2.2873550304655508E-4	0.0	1.3455029590973829E-5	1.6146035509168594E-4
88	0.7643533210040412	2.421905326375289E-4	0.0	1.3455029590973829E-5	1.6146035509168594E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGAGT	8280	0.0	86.3687	1
ATCGAGT	3555	0.0	80.94115	1
TCGAGTG	4525	0.0	69.59847	2
GTGGAAT	180370	0.0	59.472008	6
GAATCAC	182280	0.0	59.239525	9
TGGAATC	181695	0.0	59.171616	7
AGTGGAA	182025	0.0	58.972786	5
GGAATCA	182920	0.0	58.84985	8
CGAGTGG	182525	0.0	56.621304	3
ACGAGTG	177495	0.0	56.505505	2
GAGTGGA	190305	0.0	56.416805	4
AACGAGT	166250	0.0	55.66846	1
GACGAGT	2175	0.0	49.28708	1
ATGAGTG	8150	0.0	48.504486	2
TGAGTGG	8730	0.0	48.39965	3
AATGAGT	8230	0.0	44.103695	1
GAGTACT	65155	0.0	41.019592	20-21
AGTACTT	66605	0.0	40.952126	20-21
GTACTTT	66175	0.0	39.83695	22-23
CATGGGG	33545	0.0	38.77878	24-25
>>END_MODULE