FastQCFastQC Report
Thu 26 May 2016
SRR2132625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3912782
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT1585804.052870821834695No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG672611.7190070900959982No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT108220.2765807039594846No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA101000.25812835982173293No Hit
GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT64180.16402651617186953No Hit
AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC57470.14687759246490092No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT56980.1456252865608153No Hit
ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA52530.13425230437064983No Hit
CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA51190.13082763108192585No Hit
CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC49900.1275307441099453No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT49260.12589507925562937No Hit
CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT48470.12387605545108313No Hit
CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG46380.11853458741120768No Hit
TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG46200.11807455667093131No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45490.11625999097317459No Hit
CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT44750.1143687534853718No Hit
TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT43560.11132743914687811No Hit
TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA42610.10889949912875288No Hit
TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT42150.1077238650147133No Hit
TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT40340.10309800034860106No Hit
TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA39950.10210126707800231No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA39310.10046560222368636No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT35100.083.0561
ATCGAGT19600.071.250321
TCGAGTG22550.065.661622
GTGGAAT1104500.045.186276
TGGAATC1111400.045.0447047
GAATCAC1119550.044.867339
GGAATCA1117600.044.8278778
TGAGTGG40900.044.8160323
AGTGGAA1115200.044.7766575
GACGAGT12100.044.6888241
ATGAGTG39400.044.6193282
GAGTGGA1185000.042.147114
CGAGTGG1149950.041.998843
ACGAGTG1125400.041.671652
AATGAGT40400.041.2010541
AACGAGT1070950.040.7660981
AGTACTT280550.038.97928220-21
GAGTACT272900.038.94402320-21
GTACTTT274500.037.9380622-23
CATGGGG162000.037.81322524-25
Produced by FastQC (version 0.11.5)