##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2132625_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3912782 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.49510527292346 31.0 16.0 33.0 10.0 34.0 2 27.774103438423097 31.0 25.0 34.0 16.0 34.0 3 27.99650427751917 31.0 25.0 34.0 16.0 34.0 4 33.84732755364342 35.0 33.0 37.0 28.0 37.0 5 34.93993046379788 35.0 35.0 37.0 32.0 37.0 6 35.33845458295402 37.0 35.0 37.0 33.0 37.0 7 35.63715254261546 37.0 35.0 37.0 33.0 37.0 8 35.72454023761099 37.0 35.0 37.0 35.0 37.0 9 37.49363854157988 39.0 37.0 39.0 35.0 39.0 10-11 37.68741217885382 39.0 37.0 39.0 35.0 39.0 12-13 37.68787847112362 39.0 37.0 39.0 35.0 39.0 14-15 39.11274139474165 40.0 39.0 41.0 36.0 41.0 16-17 39.06925417771805 40.5 39.0 41.0 35.5 41.0 18-19 39.137553536077405 40.5 39.0 41.0 36.0 41.0 20-21 39.11308271199367 40.0 39.0 41.0 35.5 41.0 22-23 39.05368609853552 40.0 39.0 41.0 35.5 41.0 24-25 38.88061895602669 40.0 38.5 41.0 34.5 41.0 26-27 38.89891476192642 40.0 39.0 41.0 35.0 41.0 28-29 38.83653932163867 40.0 39.0 41.0 35.0 41.0 30-31 38.72543308060608 40.0 38.5 41.0 35.0 41.0 32-33 38.63596323536552 40.0 38.0 41.0 35.0 41.0 34-35 38.543185896888716 40.0 38.0 41.0 34.0 41.0 36-37 38.38132702000776 40.0 38.0 41.0 34.0 41.0 38-39 38.105264617348986 40.0 37.0 41.0 34.0 41.0 40-41 37.97952288167345 40.0 37.0 41.0 33.0 41.0 42-43 37.87627754370165 40.0 37.0 41.0 33.0 41.0 44-45 37.75809334637094 40.0 37.0 41.0 33.0 41.0 46-47 37.643261367487376 40.0 37.0 41.0 33.0 41.0 48-49 37.512543377065214 40.0 36.0 41.0 33.0 41.0 50-51 37.307936143644085 40.0 36.0 41.0 33.0 41.0 52-53 36.893784780240765 40.0 35.5 41.0 31.5 41.0 54-55 36.28682494961386 40.0 35.0 41.0 29.5 41.0 56-57 35.88805164714007 39.0 35.0 41.0 28.0 41.0 58-59 35.70715925395282 39.0 35.0 41.0 28.0 41.0 60-61 35.431976404512184 39.0 35.0 41.0 28.0 41.0 62-63 35.18291397271813 39.0 35.0 41.0 27.5 41.0 64-65 34.88148279152787 38.0 35.0 40.0 26.5 41.0 66-67 34.473169601577595 37.0 34.5 40.0 26.0 41.0 68-69 34.15548221188914 37.0 34.0 40.0 26.0 41.0 70-71 33.87335251491139 36.0 34.0 39.0 26.0 41.0 72-73 33.53763434814411 36.0 34.0 39.0 26.0 41.0 74-75 33.241385285456744 35.5 34.0 38.5 26.0 40.0 76-77 32.97054883200751 35.0 34.0 37.5 25.5 40.0 78-79 32.64104568054137 35.0 34.0 37.0 25.0 39.0 80-81 32.31611983494097 35.0 33.0 36.5 24.5 39.0 82-83 31.93753370364104 35.0 33.0 36.0 23.0 38.0 84-85 31.618501490755172 35.0 33.0 36.0 22.0 37.0 86-87 31.484726979422824 35.0 33.0 35.5 21.5 37.0 88-89 31.335317301091653 35.0 33.0 35.0 20.5 36.5 90-91 31.137898942491557 35.0 33.0 35.0 20.0 36.0 92-93 30.993650681279966 35.0 33.0 35.0 19.5 36.0 94-95 30.795981478140106 35.0 33.0 35.0 15.5 36.0 96-97 30.571048042032498 35.0 33.0 35.0 4.5 36.0 98-99 30.419092604699166 35.0 33.0 35.0 2.0 35.0 100 30.36312526483714 35.0 33.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 4.057562845025938 1101 2 4.1961698214315355 1101 3 3.4470862236660444 1101 4 1.972340437727361 1101 5 1.0742418073046167 1101 6 0.8662553875779935 1101 7 0.5044762675430476 1101 8 0.5558712032476691 1101 9 0.6588404630895823 1101 10-11 0.46143078392044146 1101 12-13 0.46612751465585234 1101 14-15 0.5730946683486948 1101 16-17 0.565247764186914 1101 18-19 0.5102129347481039 1101 20-21 0.5242281869567833 1101 22-23 0.49918098034743963 1101 24-25 0.5506269751063044 1101 26-27 0.47584629972998016 1101 28-29 0.4922616311496384 1101 30-31 0.5390943119015787 1101 32-33 0.5239945682780132 1101 34-35 0.4782947257544592 1101 36-37 0.4530206232847789 1101 38-39 0.47815480094232754 1101 40-41 0.48321567778583585 1101 42-43 0.505073566086736 1101 44-45 0.5593294600359258 1101 46-47 0.5251684854064251 1101 48-49 0.5304901650604421 1101 50-51 0.577469653927416 1101 52-53 0.633587506378646 1101 54-55 0.6157829648665682 1101 56-57 0.6917959886939897 1101 58-59 0.6149771934500876 1101 60-61 0.5976292801367222 1101 62-63 0.6018739940077751 1101 64-65 0.5910949322580592 1101 66-67 0.5276833169373205 1101 68-69 0.5895480283181627 1101 70-71 0.6050065000870362 1101 72-73 0.5515439607142554 1101 74-75 0.6571031902728208 1101 76-77 0.6232771922041849 1101 78-79 0.6130064857714359 1101 80-81 0.6066211721433774 1101 82-83 0.594927083377474 1101 84-85 0.6075870757626731 1101 86-87 0.5884918330625162 1101 88-89 0.5947074193469142 1101 90-91 0.6367494148846546 1101 92-93 0.6420305230841379 1101 94-95 0.672783453057022 1101 96-97 0.7658525458169372 1101 98-99 0.7897056088014835 1101 100 0.7737124294688087 1102 1 2.9225672433636696 1102 2 3.5255567841518314 1102 3 4.244874921454741 1102 4 2.173365705419293 1102 5 1.098899798629276 1102 6 0.8625365171924528 1102 7 0.46210662517340495 1102 8 0.5189312329743672 1102 9 0.5820424529582411 1102 10-11 0.3933991158887693 1102 12-13 0.31649606502440975 1102 14-15 0.4629678748885695 1102 16-17 0.5159044815102973 1102 18-19 0.3952146030831116 1102 20-21 0.43197229470089127 1102 22-23 0.3858282336613499 1102 24-25 0.44876602324534787 1102 26-27 0.44781675503376306 1102 28-29 0.4361206416753163 1102 30-31 0.471375077515674 1102 32-33 0.48065880827559226 1102 34-35 0.4634010441941072 1102 36-37 0.4525216394524634 1102 38-39 0.4850617078492405 1102 40-41 0.4250120195821836 1102 42-43 0.4735587179052203 1102 44-45 0.45228453632432775 1102 46-47 0.4959786895499576 1102 48-49 0.504458072361686 1102 50-51 0.4921646519557399 1102 52-53 0.500172272963411 1102 54-55 0.4200918191754184 1102 56-57 0.38021319377786966 1102 58-59 0.3258471543200443 1102 60-61 0.32211740462484073 1102 62-63 0.3348630436634963 1102 64-65 0.3301415546380113 1102 66-67 0.27311966237366647 1102 68-69 0.29443473320486646 1102 70-71 0.276773721854255 1102 72-73 0.25417689668052645 1102 74-75 0.25463557113853597 1102 76-77 0.20332542225241923 1102 78-79 0.2604038831688342 1102 80-81 0.2031131186353221 1102 82-83 0.2509194393698344 1102 84-85 0.25386062203621407 1102 86-87 0.24124155247890045 1102 88-89 0.18406647537263865 1102 90-91 0.16161367308224328 1102 92-93 0.20522401961096648 1102 94-95 0.2856068825471212 1102 96-97 0.29177847174285887 1102 98-99 0.3141861499486893 1102 100 0.33981792890763884 1103 1 2.6713003268744053 1103 2 3.340935562358432 1103 3 3.113523225906924 1103 4 1.6939689457488782 1103 5 0.863522672032083 1103 6 0.7118753464628682 1103 7 0.4288679992323807 1103 8 0.4873648949086018 1103 9 0.5128729176541569 1103 10-11 0.36906557296914144 1103 12-13 0.3306665935620998 1103 14-15 0.3888230997175697 1103 16-17 0.4412379151675623 1103 18-19 0.33960983721608784 1103 20-21 0.34120512480031806 1103 22-23 0.3496361485525128 1103 24-25 0.38511763941452415 1103 26-27 0.32401983842043336 1103 28-29 0.36199801247857266 1103 30-31 0.3230765620563858 1103 32-33 0.29934255295705725 1103 34-35 0.27836595950109455 1103 36-37 0.3290697597862149 1103 38-39 0.32776667050858777 1103 40-41 0.2908481188276255 1103 42-43 0.35823767932306794 1103 44-45 0.29979641610530905 1103 46-47 0.311237852813683 1103 48-49 0.35185708771965096 1103 50-51 0.3780180303974916 1103 52-53 0.3416084447302552 1103 54-55 0.3288525664766411 1103 56-57 0.2933159128098879 1103 58-59 0.2555066706386242 1103 60-61 0.2513254098875777 1103 62-63 0.2558933144087021 1103 64-65 0.262845101822748 1103 66-67 0.3067701900948876 1103 68-69 0.2933474459146197 1103 70-71 0.32422029769307414 1103 72-73 0.3037011784739079 1103 74-75 0.3488929465817705 1103 76-77 0.39343474999002126 1103 78-79 0.30967607440226175 1103 80-81 0.329348827311172 1103 82-83 0.31113865249668393 1103 84-85 0.3417134484295232 1103 86-87 0.32244044747482903 1103 88-89 0.3828832533228237 1103 90-91 0.3683274119510571 1103 92-93 0.3932525329530492 1103 94-95 0.40682048460450915 1103 96-97 0.40849510693156077 1103 98-99 0.43508874781822016 1103 100 0.4911118074535139 1104 1 2.6452215118465467 1104 2 3.423211188626258 1104 3 1.8182207633571998 1104 4 1.174170271232768 1104 5 0.6696173815937385 1104 6 0.5813413358838773 1104 7 0.36392035442921866 1104 8 0.4265010533580522 1104 9 0.4859362558962701 1104 10-11 0.359255710742616 1104 12-13 0.3218396174925289 1104 14-15 0.45221952731857584 1104 16-17 0.4699371882078367 1104 18-19 0.41000233752092896 1104 20-21 0.425826285946755 1104 22-23 0.40666633167424493 1104 24-25 0.42349875341303544 1104 26-27 0.37953900642230565 1104 28-29 0.3983630454858371 1104 30-31 0.3692676463138014 1104 32-33 0.3810798381150562 1104 34-35 0.42551658447261786 1104 36-37 0.3835531997080679 1104 38-39 0.44119835640447747 1104 40-41 0.45356144879579574 1104 42-43 0.4248122880151044 1104 44-45 0.4471413093602763 1104 46-47 0.4421559140035498 1104 48-49 0.49560745038940013 1104 50-51 0.5143593353362306 1104 52-53 0.5936686794032795 1104 54-55 0.5711147343486687 1104 56-57 0.5634426096656355 1104 58-59 0.49170808763602736 1104 60-61 0.49307413370566167 1104 62-63 0.5102898481685472 1104 64-65 0.5490357960658656 1104 66-67 0.44826321969768657 1104 68-69 0.5028666604121099 1104 70-71 0.47320156215055675 1104 72-73 0.382973127339703 1104 74-75 0.4159496899935675 1104 76-77 0.4418905163658735 1104 78-79 0.4865441656836538 1104 80-81 0.5464505609035939 1104 82-83 0.5193256436095091 1104 84-85 0.5300888744996897 1104 86-87 0.5666846162663681 1104 88-89 0.5367430345290245 1104 90-91 0.5877486618772032 1104 92-93 0.586418590616006 1104 94-95 0.5798334508381622 1104 96-97 0.588886336127576 1104 98-99 0.6633586911186384 1104 100 0.6783220988065928 1105 1 1.5790025580894742 1105 2 2.7477830241801264 1105 3 1.5320233458782297 1105 4 0.9858563100692805 1105 5 0.6275902500061008 1105 6 0.5616558544544645 1105 7 0.2362580171589812 1105 8 0.35115325693803356 1105 9 0.5306749689750774 1105 10-11 0.32248930387999053 1105 12-13 0.3194242561274052 1105 14-15 0.4318605947292298 1105 16-17 0.4236289066833052 1105 18-19 0.3254039022794757 1105 20-21 0.34171381204202333 1105 22-23 0.30808221234206457 1105 24-25 0.38906470589621733 1105 26-27 0.3671270942348528 1105 28-29 0.356246626213256 1105 30-31 0.38004859094497334 1105 32-33 0.34447601266155914 1105 34-35 0.28807065421076317 1105 36-37 0.3282368045474513 1105 38-39 0.3238760041506836 1105 40-41 0.27704244156761604 1105 42-43 0.34581201233075376 1105 44-45 0.35119424148403766 1105 46-47 0.35548479679064116 1105 48-49 0.3183059103258401 1105 50-51 0.38602529075532743 1105 52-53 0.26555921137412497 1105 54-55 0.3528662081355378 1105 56-57 0.4139345261174512 1105 58-59 0.32679175544137706 1105 60-61 0.30479378718875694 1105 62-63 0.001229835496225462 1105 64-65 0.10525157497617954 1105 66-67 0.23018618934700896 1105 68-69 0.3383997077610985 1105 70-71 0.34746151937367387 1105 72-73 0.2858853608118679 1105 74-75 0.23523664720876525 1105 76-77 0.23843693742498573 1105 78-79 0.25942974064584945 1105 80-81 0.2393219159462987 1105 82-83 0.21286515319537003 1105 84-85 0.20966071109466 1105 86-87 0.2666962532067352 1105 88-89 0.18802320728566713 1105 90-91 0.22882201983750505 1105 92-93 0.17330317319783362 1105 94-95 0.16618304909693649 1105 96-97 0.21659884159857867 1105 98-99 0.28615332043771247 1105 100 0.2864400242443885 1106 1 1.8886844051967273 1106 2 2.9174521979187205 1106 3 1.2826366234779982 1106 4 0.8684774616358979 1106 5 0.5587164843760632 1106 6 0.5170582815225657 1106 7 0.2502632850324318 1106 8 0.37223849124216457 1106 9 0.4177661896966356 1106 10-11 0.28488066317640914 1106 12-13 0.2190580905853068 1106 14-15 0.1831881595958862 1106 16-17 0.26163261201858745 1106 18-19 0.0660757041584148 1106 20-21 0.15298522552216554 1106 22-23 0.22889259995007194 1106 24-25 0.2728369044751915 1106 26-27 0.2161943274699638 1106 28-29 0.26787323351810244 1106 30-31 0.30120427713067244 1106 32-33 0.17682352597357465 1106 34-35 0.2292591437613396 1106 36-37 0.21274828727776196 1106 38-39 0.2201949567367123 1106 40-41 0.17132075822462411 1106 42-43 0.1959711125836776 1106 44-45 0.1420472371355146 1106 46-47 0.08711823243696415 1106 48-49 0.044404761462821796 1106 50-51 0.1950456908007041 1106 52-53 0.14189715279427162 1106 54-55 0.05341229172925921 1106 56-57 0.05368959701751663 1106 58-59 0.12155024258231606 1106 60-61 -0.012598680147611674 1106 62-63 0.17011785659587275 1106 64-65 -0.07101627166073854 1106 66-67 -0.11755904251022287 1106 68-69 0.1387916671897642 1106 70-71 0.27090250561099793 1106 72-73 0.2280908302336897 1106 74-75 0.2525379845990159 1106 76-77 0.169169315604762 1106 78-79 0.12297115328965447 1106 80-81 0.14285877066958363 1106 82-83 0.11977355974594062 1106 84-85 0.1494588718622012 1106 86-87 0.21217521473161227 1106 88-89 0.13157987993226428 1106 90-91 0.16241718820368334 1106 92-93 0.19106019913373018 1106 94-95 0.13071100803455948 1106 96-97 0.10807187901710336 1106 98-99 0.17217213341381665 1106 100 0.17409305382538065 1107 1 1.6505298433470799 1107 2 0.9385459427433318 1107 3 0.5623893824580648 1107 4 0.4165745162756451 1107 5 0.2298823825788574 1107 6 0.31368357770384847 1107 7 0.12701817215290845 1107 8 0.19954679777136874 1107 9 0.25198104853494385 1107 10-11 0.17242286851030997 1107 12-13 0.1186691800409676 1107 14-15 0.18107186735445424 1107 16-17 0.24371827393147072 1107 18-19 0.19863512651664195 1107 20-21 0.21466614564381103 1107 22-23 0.15401787435953196 1107 24-25 0.1788867989034344 1107 26-27 0.24410283730586713 1107 28-29 0.20351960239243994 1107 30-31 0.18090766918125212 1107 32-33 0.14496196986375054 1107 34-35 0.12672313573925464 1107 36-37 0.1713953275831912 1107 38-39 0.20761674540996466 1107 40-41 0.20703655605314708 1107 42-43 0.2318095553139159 1107 44-45 0.23533401550452027 1107 46-47 0.2091018361961119 1107 48-49 0.15363227427527448 1107 50-51 0.14780017249128008 1107 52-53 0.2594822191697972 1107 54-55 0.10294387801511817 1107 56-57 0.12067016987132462 1107 58-59 0.05110593314734757 1107 60-61 0.07273550325140121 1107 62-63 0.04061831194518106 1107 64-65 0.02271610255315437 1107 66-67 0.045885961135738285 1107 68-69 0.0693730817897773 1107 70-71 0.05959061598638016 1107 72-73 0.1451193547827927 1107 74-75 0.2278714738655836 1107 76-77 0.1474269719628154 1107 78-79 0.1659293891658109 1107 80-81 0.15075533231557614 1107 82-83 0.16363829174077793 1107 84-85 0.13502347004998327 1107 86-87 0.17905692804423623 1107 88-89 0.20667238924826137 1107 90-91 0.2361817470162748 1107 92-93 0.1451722059255971 1107 94-95 0.11519458200963584 1107 96-97 0.14258263308189356 1107 98-99 0.1324517988760192 1107 100 0.2134323748517879 1108 1 1.3695665522186502 1108 2 -0.7088822605964928 1108 3 0.07153172955484877 1108 4 0.05456306290530932 1108 5 0.05321611789404557 1108 6 -3.407126950847328E-4 1108 7 0.0316470318272124 1108 8 -0.04339472935159705 1108 9 0.03511266066762175 1108 10-11 0.023996294939557572 1108 12-13 0.07841505993309283 1108 14-15 0.05994320072987591 1108 16-17 0.06623672565697802 1108 18-19 0.047783519569556177 1108 20-21 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-0.4911517726047805 2215 18-19 -0.5287034325996345 2215 20-21 -0.42107005245910045 2215 22-23 -0.4743121205966432 2215 24-25 -0.46145863992048675 2215 26-27 -0.6126784995791326 2215 28-29 -0.6547579374385535 2215 30-31 -0.6717734185739985 2215 32-33 -0.7019028978155291 2215 34-35 -0.7617197985737931 2215 36-37 -0.7500108395506047 2215 38-39 -0.7831064603189262 2215 40-41 -0.8234439229914088 2215 42-43 -0.8556940642887412 2215 44-45 -1.0042586378659024 2215 46-47 -0.9237608360719207 2215 48-49 -0.9677826175260691 2215 50-51 -0.9176970814354064 2215 52-53 -1.0349339562604598 2215 54-55 -1.0647357891816043 2215 56-57 -1.0718254759078647 2215 58-59 -1.161402127046884 2215 60-61 -1.180501792112004 2215 62-63 -1.0732177644173149 2215 64-65 -1.1295619991831956 2215 66-67 -1.0476861701968687 2215 68-69 -0.9939177904417704 2215 70-71 -1.0453939003133712 2215 72-73 -1.122139526694724 2215 74-75 -1.1624173106202278 2215 76-77 -1.1426799414000044 2215 78-79 -1.1461497292671297 2215 80-81 -1.151769571541486 2215 82-83 -1.1325945559088666 2215 84-85 -1.0739179586835412 2215 86-87 -1.1318952206578707 2215 88-89 -1.147407244336378 2215 90-91 -1.151342598697557 2215 92-93 -1.1771514039409254 2215 94-95 -1.2551199405326443 2215 96-97 -1.2254622984390835 2215 98-99 -1.3269453558886966 2215 100 -1.3505098320083562 2216 1 -3.323935376524087 2216 2 -4.356244094832146 2216 3 -6.042417121349686 2216 4 -3.310208097532847 2216 5 -1.5373436327574268 2216 6 -0.7410757577165654 2216 7 -0.6056387041552185 2216 8 -0.3338269780159919 2216 9 -0.21631524952768189 2216 10-11 -0.3684671081435482 2216 12-13 -0.33753603232969454 2216 14-15 -0.5217505006020247 2216 16-17 -0.45407056660948086 2216 18-19 -0.43177523279453567 2216 20-21 -0.4005649889291547 2216 22-23 -0.4703469092063628 2216 24-25 -0.4455577720497885 2216 26-27 -0.5822674640256835 2216 28-29 -0.5896428409118712 2216 30-31 -0.7259800550021538 2216 32-33 -0.8591703272310554 2216 34-35 -0.8314593378445139 2216 36-37 -0.851674755273315 2216 38-39 -0.9592605162690049 2216 40-41 -1.0250947210053738 2216 42-43 -1.071246116069112 2216 44-45 -1.0941018164729002 2216 46-47 -1.1690258708407129 2216 48-49 -1.2250501413136021 2216 50-51 -1.1617351085491663 2216 52-53 -1.1620208751937966 2216 54-55 -1.118430972237121 2216 56-57 -1.0902288319822802 2216 58-59 -1.0856273693448628 2216 60-61 -1.0524391953324326 2216 62-63 -1.064784853604614 2216 64-65 -1.1049476782656953 2216 66-67 -1.095468732635318 2216 68-69 -1.0399632216399084 2216 70-71 -1.0727498137790406 2216 72-73 -1.1113006873983196 2216 74-75 -1.1541602749083992 2216 76-77 -1.0583671767884084 2216 78-79 -1.0596653361233095 2216 80-81 -1.014643487368005 2216 82-83 -1.0410204231382636 2216 84-85 -1.0418775904577018 2216 86-87 -1.0610679582327478 2216 88-89 -1.0721134406907176 2216 90-91 -1.230661625749633 2216 92-93 -1.2115016556868312 2216 94-95 -1.1835558472275594 2216 96-97 -1.2646991326220665 2216 98-99 -1.3398391456016157 2216 100 -1.4061985807805542 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1984.0 3 59.0 4 114.0 5 211.0 6 439.0 7 745.0 8 1335.0 9 2122.0 10 3541.0 11 6284.0 12 6410.0 13 8297.0 14 8414.0 15 8942.0 16 9995.0 17 12032.0 18 16499.0 19 26543.0 20 47565.0 21 56538.0 22 28770.0 23 20212.0 24 20957.0 25 23088.0 26 26501.0 27 30946.0 28 38902.0 29 48923.0 30 60368.0 31 75696.0 32 96633.0 33 127019.0 34 176114.0 35 273012.0 36 458849.0 37 816833.0 38 1023752.0 39 345717.0 40 2421.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 16.942489014527233 37.279411783502916 27.51978203145032 18.25831717051954 2 19.863462403085222 34.3153911647001 26.809989650665884 19.01115678154879 3 21.060210367476003 24.44863775379021 21.187977657720655 33.30317422101313 4 29.939132821608766 26.047144972554054 23.919937272252838 20.09378493358434 5 18.235746047291926 34.442040834895366 24.83728352818076 22.484929589631946 6 33.309777820827996 22.29167619749112 25.831852488139397 18.566693493541486 7 20.62429972847866 22.703748343883408 36.56322071740517 20.108731210232754 8 32.41489047945937 22.63264347464285 25.299237217918098 19.65322882797968 9 32.25263565734959 24.76067533552223 25.102529377442796 17.88415962968538 10-11 18.237522560674222 37.89262984751004 24.890563798340924 18.97928379347482 12-13 18.15464035563443 24.335613381987546 31.45678701241214 26.05295924996588 14-15 18.41178476081724 30.96341682209742 24.554409113515653 26.070389303569684 16-17 25.49209232714728 23.985529988637243 31.285872813767803 19.236504870447675 18-19 18.85052630072414 24.18748348361856 31.33113472715832 25.630855488498977 20-21 25.71095195183376 30.98765788638365 24.6891597845216 18.61223037726099 22-23 25.146059760037744 24.27656332502041 31.53223205381746 19.045144861124385 24-25 18.552422394425612 30.935348420503683 24.565289813393417 25.946939371677292 26-27 18.800692724425563 27.83108043695004 34.36198378241747 19.00624305620693 28-29 26.6359347601682 24.01115273273643 30.50101680065979 18.85189570643558 30-31 23.94934219118306 25.86075364135267 30.88308696437786 19.306817203086414 32-33 20.413158093486498 26.807648126184546 31.271321239216142 21.507872541112814 34-35 21.083216368641526 26.613866740890202 31.650319724790165 20.652597165678106 36-37 21.398435286598954 25.707473659149798 32.67181694839098 20.222274105860258 38-39 22.697673146626357 25.77393838856321 31.556344289087452 19.97204417572298 40-41 20.09954770560788 28.224846747447152 31.459201491835394 20.216404055109578 42-43 20.08978817642895 25.793288852032003 32.59698798483449 21.519934986704556 44-45 20.119397476484213 26.982797091766397 31.154462721579524 21.743342710169873 46-47 21.43385228901155 25.751688264153515 32.301914194607804 20.51254525222713 48-49 20.323213444256048 26.001149988363366 32.208173199136496 21.46746336824409 50-51 21.638338543764423 27.068080835203332 31.088564542217277 20.205016078814964 52-53 21.412033413126345 26.14592600042761 31.804682249286216 20.637358337159828 54-55 20.661734731041676 27.741665058611996 29.659624878117974 21.936975332228357 56-57 20.934461824358042 28.063118720941272 29.934416714364414 21.06800274033627 58-59 23.40731071830762 27.300625022044716 28.428509429515948 20.86355483013172 60-61 21.92647427578639 28.616096430430993 28.389242497013832 21.06818679676878 62-63 20.696548098091483 28.53367591040274 28.196151273404666 22.573624718101108 64-65 21.74260882678644 28.62330273154068 28.377785488398604 21.25630295327428 66-67 22.03159577366654 27.322998388210678 29.535885955042684 21.109519883080097 68-69 23.063552893354515 27.434574671467626 28.783004034195585 20.718868400982274 70-71 20.440620238965135 29.55254291177193 29.01667450029021 20.99016234897272 72-73 20.316232879749542 27.38466941049868 30.018929603930015 22.28016810582176 74-75 20.513416863291937 28.433317453372208 28.6023316962661 22.45093398706976 76-77 21.902548847869852 27.292919049180504 29.516276319041364 21.288255783908287 78-79 20.94421674717962 27.68368945986358 29.12621371629922 22.24588007665758 80-81 22.04948331247398 28.781014605466847 28.10281510552908 21.066686976530097 82-83 21.832436219101186 27.86538353901661 29.014363624793017 21.287816617089188 84-85 20.80933054585789 29.11114717563651 27.631455195080683 22.448067083424913 86-87 20.970264700823485 29.136514227691624 28.678576257099536 21.214644814385355 88-89 23.31008785930448 28.01373106726168 27.54559281198078 21.130588261453056 90-91 22.011922560858732 29.311277234681487 27.465503801674014 21.211296402785766 92-93 21.16140917264624 29.191129339771066 27.07685666705173 22.57060482053096 94-95 21.988427083109706 29.535204853049873 27.06184592139147 21.414522142448956 96-97 22.294246276774103 28.337677267262602 28.063278483393717 21.304797972569578 98-99 23.398072966970954 28.665760034063897 26.856486158858694 21.079680840106455 100 21.187597037907878 30.663352735590472 26.844221811885426 21.304828414616225 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 11700.0 1 7312.5 2 2366.5 3 1606.0 4 1366.5 5 1291.5 6 1195.0 7 1193.5 8 1185.5 9 1200.5 10 1311.5 11 2110.5 12 14545.5 13 16923.0 14 7159.0 15 7096.5 16 8172.5 17 9752.5 18 11298.0 19 12771.5 20 14744.5 21 17114.5 22 20237.5 23 24463.5 24 28786.0 25 33298.5 26 39390.0 27 45862.0 28 51169.5 29 55817.0 30 61106.0 31 66596.0 32 71228.5 33 76075.0 34 81384.0 35 85869.0 36 90518.5 37 95582.5 38 99321.5 39 102681.0 40 106307.0 41 109064.0 42 110905.0 43 113232.0 44 115594.5 45 118068.0 46 122111.0 47 129320.0 48 135874.0 49 162222.5 50 245884.5 51 244129.5 52 150030.0 53 109217.0 54 98222.0 55 90799.0 56 83542.0 57 76607.0 58 70331.5 59 60037.0 60 47128.0 61 37262.0 62 29064.0 63 22087.5 64 15948.5 65 11040.5 66 7315.5 67 4806.5 68 3193.0 69 2067.5 70 1361.0 71 940.5 72 603.5 73 403.0 74 293.5 75 215.5 76 160.5 77 112.0 78 84.5 79 58.5 80 34.5 81 34.5 82 27.0 83 18.5 84 14.0 85 12.5 86 8.5 87 6.5 88 7.0 89 4.5 90 4.5 91 2.5 92 2.0 93 2.0 94 3.0 95 2.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026196194932403594 2 0.011577440296954953 3 0.005111452669737287 4 0.0 5 0.003782474975605592 6 9.96733270598771E-4 7 3.5780168688161006E-4 8 0.0 9 2.5557263348686434E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 8.050537954836226E-4 26-27 0.00293908528509894 28-29 4.8558800362504223E-4 30-31 4.8558800362504223E-4 32-33 0.004178612557510232 34-35 0.007066583315911799 36-37 0.004613086034437901 38-39 0.004613086034437901 40-41 0.0073093773177243205 42-43 0.00898337806706328 44-45 0.007769408058000676 46-47 0.010363470287892348 48-49 0.014529304213728238 50-51 0.060238469712853925 52-53 0.009392294280642264 54-55 0.0047792082462043625 56-57 0.0031563220235627746 58-59 0.007156033737632202 60-61 0.014644311898797327 62-63 0.012139700090626055 64-65 0.010976844608260823 66-67 0.009200614805527115 68-69 0.00587817057019788 70-71 0.004114719399138516 72-73 0.0032585510769575204 74-75 0.002402382754776525 76-77 0.0018656802244541096 78-79 0.00146954264254947 80-81 0.0017634511710593638 82-83 0.0013034204307830082 84-85 0.0010734050606448302 86-87 6.389315837171609E-4 88-89 0.0012523059040856353 90-91 8.178324271579659E-4 92-93 4.600307402763558E-4 94-95 6.644888470658472E-4 96-97 0.0011756341140395758 98-99 0.0034502305520726687 100 1.7890084344080503E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3912782.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.7446112530721 #Duplication Level Percentage of deduplicated Percentage of total 1 87.02307831278476 58.083215320324676 2 9.047311477528162 12.077185749061488 3 1.9865128574634812 3.9776708526188838 4 0.696906984592265 1.8605914306464573 5 0.33851752921020967 1.129711044474296 6 0.207773379446107 0.8320652063920471 7 0.1325118138222999 0.619111465000621 8 0.08957179735479334 0.4782747834947707 9 0.06374075960519383 0.38289169987417654 >10 0.3727566987253468 5.363861193864508 >50 0.022924059025939275 1.0083536585244146 >100 0.014457370490934799 1.890156709011559 >500 0.0011849102954351106 0.5359250045212639 >1k 0.0024080434482037848 4.745310071771549 >5k 1.9111455938125268E-4 0.7203238078356476 >10k+ 1.5289164750500214E-4 6.2953520025835985 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 158580 4.052870821834695 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG 67261 1.7190070900959982 No Hit TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 10822 0.2765807039594846 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 10100 0.25812835982173293 No Hit GTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGT 6418 0.16402651617186953 No Hit AGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTC 5747 0.14687759246490092 No Hit AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 5698 0.1456252865608153 No Hit ACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTGA 5253 0.13425230437064983 No Hit CACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCA 5119 0.13082763108192585 No Hit CCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTC 4990 0.1275307441099453 No Hit ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT 4926 0.12589507925562937 No Hit CCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGAT 4847 0.12387605545108313 No Hit CTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCG 4638 0.11853458741120768 No Hit TTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTG 4620 0.11807455667093131 No Hit ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT 4549 0.11625999097317459 No Hit CCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATT 4475 0.1143687534853718 No Hit TCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGT 4356 0.11132743914687811 No Hit TCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGA 4261 0.10889949912875288 No Hit TACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCAGTGATTCCACT 4215 0.1077238650147133 No Hit TCCACTCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACT 4034 0.10309800034860106 No Hit TCGTTCCCATGTACTCAGCAGTGATTCCACTCGTTCCCATGTACTCAGCA 3995 0.10210126707800231 No Hit AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA 3931 0.10046560222368636 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.5557263348686434E-4 2.5557263348686434E-5 0.0 0.0 0.0 2 3.322444235329236E-4 2.5557263348686434E-5 0.0 0.0 0.0 3 3.833589502302965E-4 5.111452669737287E-5 0.0 0.0 0.0 4 3.833589502302965E-4 1.0222905339474574E-4 0.0 0.0 0.0 5 4.0891621357898295E-4 1.0222905339474574E-4 0.0 0.0 0.0 6 4.0891621357898295E-4 1.2778631674343217E-4 0.0 0.0 0.0 7 4.600307402763558E-4 1.533435800921186E-4 0.0 0.0 0.0 8 4.600307402763558E-4 1.533435800921186E-4 0.0 0.0 0.0 9 4.8558800362504223E-4 1.533435800921186E-4 0.0 0.0 0.0 10-11 5.367025303224151E-4 1.9167947511514825E-4 0.0 0.0 0.0 12-13 5.750384253454448E-4 2.300153701381779E-4 0.0 0.0 0.0 14-15 6.51710215391504E-4 2.5557263348686434E-4 0.0 0.0 0.0 16-17 7.028247420888769E-4 2.811298968355508E-4 0.0 0.0 0.0 18-19 7.922751638092795E-4 2.811298968355508E-4 0.0 0.0 0.0 20-21 8.433896905066523E-4 2.811298968355508E-4 0.0 0.0 0.0 22-23 9.45618743901398E-4 3.066871601842372E-4 0.0 0.0 0.0 24-25 0.0010989623239935167 3.1946579185858045E-4 0.0 0.0 0.0 26-27 0.0012395272724112921 3.322444235329236E-4 0.0 0.0 0.0 28-29 0.0013417563258060377 3.4502305520726684E-4 0.0 0.0 0.0 30-31 0.0015589930642698725 3.833589502302965E-4 0.0 0.0 0.0 32-33 0.0020190238045462282 4.0891621357898295E-4 0.0 0.0 0.0 34-35 0.0025046118081712707 4.216948452533261E-4 0.0 0.0 0.0 36-37 0.0028624134950528805 4.4725210860201256E-4 0.0 0.0 0.0 38-39 0.0035780168688161005 4.600307402763558E-4 0.0 0.0 0.0 40-41 0.004855880036250423 4.8558800362504223E-4 0.0 0.0 0.0 42-43 0.006465987627217668 5.494811619967583E-4 0.0 0.0 0.0 44-45 0.008216660166602689 5.622597936711016E-4 0.0 0.0 1.2778631674343217E-5 46-47 0.01024846260282326 6.133743203684745E-4 0.0 0.0 2.5557263348686434E-5 48-49 0.012778631674343217 6.644888470658472E-4 0.0 0.0 2.5557263348686434E-5 50-51 0.016152190436369826 6.644888470658472E-4 0.0 0.0 2.5557263348686434E-5 52-53 0.020190238045462282 6.772674787401905E-4 0.0 0.0 2.5557263348686434E-5 54-55 0.025007782186689677 7.283820054375633E-4 0.0 0.0 2.5557263348686434E-5 56-57 0.03087317412521321 7.922751638092795E-4 0.0 0.0 2.5557263348686434E-5 58-59 0.03800365059949673 8.178324271579659E-4 0.0 0.0 2.5557263348686434E-5 60-61 0.04855880036250422 8.945042172040251E-4 0.0 0.0 2.5557263348686434E-5 62-63 0.06252584478256136 9.328401122270548E-4 0.0 0.0 2.5557263348686434E-5 64-65 0.08069705902347742 9.711760072500845E-4 0.0 1.2778631674343217E-5 2.5557263348686434E-5 66-67 0.10349413793050571 9.711760072500845E-4 0.0 2.5557263348686434E-5 2.5557263348686434E-5 68-69 0.13357503689190964 0.0010222905339474573 0.0 2.5557263348686434E-5 2.5557263348686434E-5 70-71 0.17258819939367948 0.0010222905339474573 0.0 2.5557263348686434E-5 2.5557263348686434E-5 72-73 0.22345993208923984 0.0010350691656218006 0.0 2.5557263348686434E-5 2.5557263348686434E-5 74-75 0.28648414350710055 0.0010989623239935167 0.0 2.5557263348686434E-5 2.5557263348686434E-5 76-77 0.3648171556708245 0.0011628554823652328 0.0 2.5557263348686434E-5 2.5557263348686434E-5 78-79 0.46391544430535614 0.0012139700090626057 0.0 2.5557263348686434E-5 3.833589502302965E-5 80-81 0.5876253775446728 0.0012523059040856351 0.0 2.5557263348686434E-5 5.111452669737287E-5 82-83 0.7409434003734428 0.0012650845357599784 0.0 2.5557263348686434E-5 5.111452669737287E-5 84-85 0.9289426295663801 0.0013161990624573512 0.0 2.5557263348686434E-5 5.111452669737287E-5 86-87 1.150434652377771 0.0013289776941316945 0.0 2.5557263348686434E-5 5.111452669737287E-5 88 1.3377949499869914 0.001354534957480381 0.0 2.5557263348686434E-5 5.111452669737287E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACGAGT 3510 0.0 83.056 1 ATCGAGT 1960 0.0 71.25032 1 TCGAGTG 2255 0.0 65.66162 2 GTGGAAT 110450 0.0 45.18627 6 TGGAATC 111140 0.0 45.044704 7 GAATCAC 111955 0.0 44.86733 9 GGAATCA 111760 0.0 44.827877 8 TGAGTGG 4090 0.0 44.816032 3 AGTGGAA 111520 0.0 44.776657 5 GACGAGT 1210 0.0 44.688824 1 ATGAGTG 3940 0.0 44.619328 2 GAGTGGA 118500 0.0 42.14711 4 CGAGTGG 114995 0.0 41.99884 3 ACGAGTG 112540 0.0 41.67165 2 AATGAGT 4040 0.0 41.201054 1 AACGAGT 107095 0.0 40.766098 1 AGTACTT 28055 0.0 38.979282 20-21 GAGTACT 27290 0.0 38.944023 20-21 GTACTTT 27450 0.0 37.93806 22-23 CATGGGG 16200 0.0 37.813225 24-25 >>END_MODULE