FastQCFastQC Report
Thu 26 May 2016
SRR2132624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2132624_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3966755
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT2490286.277877005259967No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA376200.9483822419080584No Hit
TACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT173230.43670456078078934No Hit
AATGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT89700.22612941812640308No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGGAGGCATTCAGGCAGCGAGA88490.22307906588634793No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78170.19706283851662126No Hit
ATCGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76280.1922982387367004No Hit
AACGAGTGGAATCACTGCTGAGTACATGGGAACGAGTGGAATCACTGCTG72020.18155898208989463No Hit
ACGAGTGGAATCACTGCTGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43270.10908160448527826No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGAGT43550.089.71371
AACGAGT613850.084.473361
ACGAGTG672500.083.602732
CGAGTGG704700.082.326773
GTGGAAT743250.080.662026
GAGTGGA744950.080.518834
ATCGAGT20850.080.051261
TGGAATC753200.079.826297
AGTGGAA754050.079.578285
GAATCAC762950.079.231149
GGAATCA759750.079.193748
TCGAGTG24850.073.007912
GACGAGT12000.061.904431
ACGATTG5950.048.9760482
ATGAGTG40650.048.3308832
TGAGTGG44350.047.472123
CGAGTTG6450.042.9879263
AATGAGT43300.042.0212481
GAGTACT417300.041.14991420-21
AGTACTT422600.041.14537420-21
Produced by FastQC (version 0.11.5)